Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

Effect of growth regulator treatment on bud sprouting of hardwood cutting in different ornametal plants

The hardwood cuttings of Calliandra haematocephala, Cassia biflora, Pyrostegia venusta and Clerodendrum splendens were treated with different growth regulator concentrations and combinations (T1: NAA100 mg/l, T2: NAA 300 mg/l, T3: NAA 500 mg/l, T4: IBA100 mg/l, T5: IBA 300 mg/l, T6: IBA 500 mg/l, T7: NAA 100 mg/l + IBA 50 mg/l, T8: NAA 50 mg/l + IBA100 mg/l, T9: NAA 100 mg/l + IBA100 mg/l and T10: Control) for 12 h and planted either in polybags containing soil or in sand beds for callusing. The treatment of hardwood cuttings with T6: IBA (500 mg/l) for 12h resulted in the maximum (33.33%) mean per cent sprouting 60 days after direct planting in all the four genotypes. Among the genotypes, the per cent sprouting was significantly more in C haematocephala (47.33%), followed by P venusta (8.66%), C splendens (7.33%) and C biflora (7.33%), irrespective of the growth regulator treatment. The hard-wood cuttings, planted in the sand beds for callusing (2 weeks) exhibited the maximum (28.33%) mean per cent sprouting with T6: IBA (500 mg/l, 12h), 60 days after transplanting in the polybags in all the four genotypes. Among the geno-types, the per cent sprouting was significantly more in C haematocephala (48.67%), followed by P venusta (6.67%) and C splendens (2.67%), irrespective of the growth regulator treatment, however, the cuttings failed to exhibit sproutingin C biflora. The treatment of cutting with IBA 500 mg/l increase the sprouting percentage in C haematocephala, P venusta and C splendens which otherwise were difficult to propagate through cutting.

Pengaruh Asal Bahan Stek dan Jenis Zat Pengatur Tumbuh (Zpt) pada Pertumbuhan Stek Daun Lidah Mertua (Sansevieria Trifasciata)

This research aims to study the growth response of plant leaf cuttings mother in-law�s tongue (Sansevieria parva) origin of cuttings and various plant growth regulator treatment. Research was using complete randomized block design (RAKL) with 6 replications. The first factor was the origin of cuttings, i.e. the top/tip leaves, the middle leave, and the bottom/leaves base. The second factor was the various plant growth regulator (PGR) treatment i.e. onion extracts, water coconut and Rootone-F. Parameters observed and measured is the experiment root length, number of roots, root wet weight and root dry weight. The results showed that plant growth regulator treatment significant effect on root length, number of roots, root wet weight, and root dry weight. The treatment combination of origin of cuttings and synthetic plant growth regulator by cutting material from the middle to give the best results on the number of root and root wet weight.

Analysis of Cutting Growth Characteristics in Robusta Coffee(Coffea canephora Pierre.)

Development of Robusta coffee clones needs special characteristics for rootstock. This research was aimed to study the characteristics of cutting growth of Robusta coffee as influenced by genetic factor. The research was conducted at Indonesian Coffee and Cocoa Research Institute using 269 progenies originated from reciprocal crossing populations among three parentals, namely BP 409, BP 961, and Q 121. BP 308 an easy cutting-propagated genotype was used as control. The research was arranged in three replications of randomized completely block design with 10 cuttings per replication. Each cutting was single planted in plastic polybag of 15 cm x 25 cm without any growth-regulator treatment. Cluster analysis procedure showed root growth characteristics which could be divided into three groups namely easy (85.3%, 3.82 and 6.68 cm), moderate (57.6%, 1.73 and 4.01 cm) and difficult (25.1%, 0.58 dan 1.44 cm) based on proportion of rooted cuttings, number of primary root and length of root, respectively. On the other side, growth of sprout showed good homogenous characteristic, mainly indicated by proportion of sprouted cuttings which generally achieved up to 98% in average. Study on shoot-root ratio of cuttings resulted in two groups of progenies, namely the first which tended to be dominant on sprout growth and the second which tended to balance their root and sprout growth. Based on proportion of sprouted cuttings which almost achieved up to 100% and various proportion of rooted cutting, it could be indicated that growth of cuttings was started and dominated by the growth of sprout. Key words: Coffee canephora, progeny, genetic variation, cutting, rooting, sprouting

Impact of Augeo, Configure and Florel on Hydrangea Branching

Hydrangeas are an increasingly popular group of shrubs which are prized for their showy inflorescence. Nursery crop producers grow numerous hydrangea varieties for the landscape and florist markets. Hydrangeas are typically hand-pruned during propagation and production to promote compaction and to increase the number of branches which has potential for increasing blooms and marketability. Identifying a plant growth regulator (PGR) treatment that effectively improves the architecture of hydrangeas became an IR-4 Project research priority for 2011. Research evaluating Augeo (dikegulac sodium), Configure (benzyladenine) and Florel (ethephon) shows worthwhile results. Augeo (800 and 1600 ppm) significantly increased branching compared to untreated pots of florist varieties Hydrangea macrophylla ‘Merritt's Supreme’ and landscape varieties H. macrophylla ‘Nikko Blue’ and H. paniculata ‘Limelight’. Configure (300 ppm and 600 ppm) and Florel (500 and 1000 ppm) did not significantly improve branching in five of six trials compared to the control. Significant chlorosis on Augeo treated plants was observed initially but this diminished by 6 weeks after treatment (WAT) for four out of six trials. Little to no crop injury was observed with Configure or Florel treatments to hydrangea liners. This research shows promise for identifying a plant growth regulator treatment to meet the demand for improved branching on hydrangea.

Effect of gibberellin, auxin and kinetin treatments combined with foliar applied NPK on the yield of Capsicum annuum L. fruits and their capsaicin content

The Wrocław version of hydroponic culture was applied. Under optimal conditions of root fertilization the plants were sprayed with growth regulators sueh as gibberellins, auxins and kinetins, and .their mixtures. Each growth regulator treatment was applied with or without NPK added. The influence of these treatments on the fresh and dry weight of the fruit, percentage of ripe fruits and content and yield of capsaicin was studied. The highest yield of fruits and capsaicin. was obtained from plants sprayed with gibberellic acid and kinetin (in concentrations of 10 and 5 mg/l, respectively) together with NPK foliar application. No influence of ,growth regulators and foliar-applied NPK was noted on capsaicin content and dry weight of fruits.