Vapor-phase chromatography in glass-capillary column of high polar and involatile hydrocarbon-containing ether derivatives of disulpho-benzoic acid

The paper deals with the results of capillary columns implementation in vapor-phase chromatography. Glass and narrow-bore columns covered with liquid stationary phase and polyphenyl ether were used, the mixtures of aliphatic alcohols, acids, amines and ether derivatives of disulpho-benzoic acid, which are semi-products of additive synthesis analyzed. The usage of water vapor as a moving carrier of nitrogen enabled to decrease the analysis time and improve division efficiency. The retention time, height values of equivalent theoretic plates and coefficient of analyzed components asymmetry were calculated. Obtained results justify the practicability of glass capillary application for the analysis of disulpho-benzoic acid derivatives via vapor-phase chromatography method.

Trace-Level Detection of Atrazine Using Immuno-Chemiluminescence: Dipstick and Automated Flow Injection Analyses Formats

A sensitive chemiluminescence (CL)-based immunoassay technique based on both dipstick and flow injection analytical formats is reported for the detection of atrazine. In the dipstick-based immunoassay technique, antibody (anti-atrazine) was first immobilized on the nitrocellulose membranes. The dipstick was then treated with atrazine and atrazine-horseradish peroxidase conjugate (atra-HRP) to facilitate the competitive binding. The dipstick was further treated with urea-hydrogen peroxide (U-H2O2) and luminol to generate photons. The number of photons generated was inversely proportional to the atrazine concentration. In the flow injection analysis (FIA) format, the antibody was immobilized on protein-A sepharose matrix and packed in a glass capillary column, which functioned as an immunoreactor. Competitive binding of antigen and antibody occurred. The CL signals generated during the biochemical reactions were correlated with atrazine concentrations in the analytical samples. By using dipstick technique, it was possible to detect atrazine concentration down to 0.1 ng/mL; with the FIA format, the detection of atrazine was down to 0.01 ng/mL.