Expression, Microencapsulation of Recombinant Human Epidermal Growth Factor, and Release Study in Gastric Ulcer Representing Condition

Recombinant human epidermal growth factor (rhEGF) has been studied and expressed in various expression systems. It has been also commercialized and clinically used, yet limited to topical diseases. However, being naturally expressed in different tissues, the rhEGF is potential to be applied not only for external wound and skin disorders, but also to regenerates internal damaged epidermal cells such found in gastric ulcer. In the recent study, chitosan microparticles were developed to facilitate delivery of the rhEGF and to overcome gastric degradation that majorly interfere protein, particularly rhEGF oral administration. The rhEGF was expressed in E. coli BL21(DE3) and purified using Ni-NTA chromatography. The refolded rhEGF showed proliferation activity on MC7 cells. rhEGF loaded chitosan microparticles were stable in the gastric and specifically released the loaded rhEGF in the high oxidative environment in acidic pH representing gastric ulcer condition.

Use of Triamcinolone Acetonide in External Dacryocystorhinostomy

Purpose: To study the effect of Triamcinolone Acetonide injection into the ostium through the external wound after one week of external Dacryocystorhinostomy. Study Design: Quasi experimental study. Place and Duration of Study: Khalid Eye Clinic, Karachi, from July 2018 to June 2019. Methods: This study included forty patients with age ranging between 18 to 36 years. All patients had obstruction of the nasolacrimal duct. Patients were categorized into two groups. Both groups underwent external dacryocystorhinostomy with silicone intubation, with the difference being that patients in group A were injected with 20 units of Triamcinolone acetonide 40 mg/ml into the ostium through wound using 27 gauge needle, one week after the surgery. Group B did not receive any injection. Both groups were followed for a period of four months, at which time the silicone tube was removed and patency of lacrimal system was ascertained via syringing with balanced salt solution through the lacrimal puncta. Main outcome measure was the success rate of procedure. Results: The mean age of the patients was 27.1 ± 5.48 years. Success rate was 100% among the patients of group A and 85% in the patients of group B at the end of the four months followup period. However, the results were statistically insignificant (p = 0.115). Conclusion: Although statistically insignificant but clinically Triamcinolone Acetonide proved helpful in reducing fibrosis and ostium granuloma formation in patients with Ex-DCR. Thus preventing failure of external Dacryocystorhinostomy. Key Words: Triamcinolone acetonide, external dacryocystorhinostomy, DCR.

Polymeric Materials for Hemostatic Wound Healing

Hemorrhage is one of the greatest threats to life on the battlefield, accounting for 50% of total deaths. Nearly 86% of combat deaths occur within the first 30 min after wounding. While external wound injuries can be treated mostly using visual inspection, abdominal or internal hemorrhages are more challenging to treat with regular hemostatic dressings because of deep wounds and points of injury that cannot be located properly. The need to treat trauma wounds from limbs, abdomen, liver, stomach, colon, spleen, arterial, venous, and/or parenchymal hemorrhage accompanied by severe bleeding requires an immediate solution that the first responders can apply to reduce rapid exsanguinations from external wounds, including in military operations. This necessitates the development of a unique, easy-to-use, FDA-approved hemostatic treatment that can deliver the agent in less than 30 s and stop bleeding within the first 1 to 2 min at the point of injury without application of manual pressure on the wounded area.

An Atypical Type of Monteggia Equivalent: A Case Report

Introduction:Management of Mmonteggia fractures has always been debatable; Nnumerous variants have been described in the literature since its first description in 1814. We report a new variant of classical Mmonteggia fracture in a 33-year-old male. Case Presentation: A 33-year old male was brought to the emergency room with an alleged history of assault and was managed with an external wound. Antero-Pposterior and lateral radiographs showed segmental ulna fracture and radial head dislocation. The Ppatient was managed surgically with open reduction internal fixation with reconstruction plate for segmental ulna and closed reduction of radial head was attempted after achieving an anatomical reduction of the ulna. Close attention was paid to the early post-operative mobilization of the elbow joint with the help of a Hinged elbow brace and terminal functional outcome re re-evaluated at 6six months with a DASH score of 11 and MAYO score of 92. Conclusion: We report a rare type 1 monteggia equivalent not described in the literature. Despite the variability of the monteggia equivalents, the principle of management remains the same. Early mobilization of the elbow is of utmost importance to avoid post-operative stiffness. Keywords: Atypical, Equivalent, Monteggia, Segmental.

Treatment of phalangeal osteomyelitis caused by a cat bite

In situation of a cat bite, although an external wound is small, prophylactic antibiotics should be used early and a closed observation is needed for sufficient periods. If symptoms continue, deep infection should be considered.

Penggunaan Alkohol untuk Kepentingan Medis Tinjauan Istihsan

<div><table cellspacing="0" cellpadding="0" align="center"><tbody><tr><td align="left" valign="top"><p>This research discusses analytical studies regarding the use of alkohol in the medical world, in which alkohol in the Qur'an is haram. However, this thesis describes using the istihsan method which can change the law of origin from alkohol if it is used in the medical world, such as disinfectants and as a washing machine for medical devices. The purpose of this research is to examine the method of istihsan which can change the law of origin of alkohol.</p><p>The research method used in this research is literary qualitative which requires a lot of library data. This is where the study in this study discusses the use of alkohol in the medical world. So the source of this research is able to present an analysis of the use of alkohol for medical purposes using the istihsan method.</p></td></tr></tbody></table></div>The results of research on the use of alkohol for medical purposes istihsan review. Alkohol is used in medical use only outside the body, as a disinfectant and also as a detergent for medical devices and as an external wound wash.

Surface Modification of Film Chitosan Materials With Aldehydes for Wettability and Biodegradation Control

Chitosan is one of prospective polymer for use in regenerative medicine which has unique properties such as biocompatibility, biodegradability, antimicrobial, anti-inflammatory, and antitumor potency. In this article, we study the peculiarities of the surface modification of chitosan films with carbonyl-containing compounds, which differed both in molecular characteristics and in their hydrophilic and hydrophobic properties. The potential for controlling the biodegradation of the resulting materials has been established, which can be used in the creation of wound dressings. Both the destruction time and lyophilic properties of the surface depend on the length of the modifier's hydrocarbon radical. The contact angle and water absorption of obtained film materials correlate with hydrophobicity, which estimated by the calculated value of the hydrophilic–lipophilic balance (HLB). The cytotoxicity of modified chitosan films was studied, and it was found that they are non-toxic (cytotoxic index of <50%) for human skin cell cultures, which shows their potential for use in the creation of materials for skin protection and external wound healing.

C-Reactive Protein Triggers Cell Death in Ischemic Cells

C-reactive protein (CRP) is the best-known acute phase protein. In humans, almost every type of inflammation is accompanied by an increase of CRP concentration. Until recently, the only known physiological function of CRP was the marking of cells to initiate their phagocytosis. This triggers the classical complement pathway up to C4, which helps to eliminate pathogens and dead cells. However, vital cells with reduced energy supply are also marked, which is useful in the case of a classical external wound because an important substrate for pathogens is disposed of, but is counterproductive at internal wounds (e.g., heart attack or stroke). This mechanism negatively affects clinical outcomes since it is established that CRP levels correlate with the prognosis of these indications. Here, we summarize what we can learn from a clinical study in which CRP was adsorbed from the bloodstream by CRP-apheresis. Recently, it was shown that CRP can have a direct effect on blood pressure in rabbits. This is interesting in regard to patients with high inflammation, as they often become tachycardic and need catecholamines. These two physiological effects of CRP apparently also occur in COVID-19. Parts of the lung become ischemic due to intra-alveolar edema and hemorrhage and in parallel CRP increases dramatically, hence it is assumed that CRP is also involved in this ischemic condition. It is meanwhile considered that most of the damage in COVID-19 is caused by the immune system. The high amounts of CRP could have an additional influence on blood pressure in severe COVID-19.

Management of Chronic Non-Healing Wound using Ayurvedic drugs - A Case Study

Sushruta in 1st part of his compendium described about wound healing its types etc. as Vranitopasaniya adhyaya. Ayurveda described so many compound medicines and oil for oral administration and topical application to accelerate the wound healing. But this is not scientifically validated. So there is always a search for finding a newer and better medicine for wound healing. I am presenting a case of 42 year old married male with complain of superficial external wound with mild pain, mal odour and not healing since 45 days in spite of taking allopathic conventional treatment in his lower limb. The study was done in my outpatient department of Central Research institute for cardiovascular disease, Punjabi Bagh, New Delhi. On examination it was found that surface area of superficial wound is 22.5 sq. cm. having full thickness, skin loss involving necrosis of subcutaneous tissue extended down to underlying fascia. Necessary laboratory investigations were done prior to initiate the treatment. The wound was irrigated with Panchvalkala kasaya and dressing with Jatyadi Tail was done daily followed by oral intake of Triphala guggulu 500mg twice in a day and Amalaki churna 6gm twice in a day was administered orally for 28th days. Periodic follow up was done on 0, 7, 14, and in 28 days. This regimen was found to be effective in faster wound epithelialization, and reducing wound exudates. There was no evidence of any allergic reaction as well as no evidence showing any adverse event on the wound.