The purpose of this paper is, through content analysis of 19 publications in the Greek and international literature in scientific texts, books, journal articles, and conferences, to investigate the conceptual content of pedagogical differentiation in higher education, as it emerges from the descriptions and discussion of authors, researchers, and experts. From the analysis, twelve dimensions or characteristics of pedagogical differentiation emerged that presented the highest frequency of occurrence in four broad categories. These are a. modification of the supportive learning context, meeting the needs of learners, and continuous improvement of the learning for all the learners who joined the category entitled "processes", b. student-centered teaching and learning, flexible learning context / flexible grouping and the possibility of learning option / multiple options as dimensions of a more general category called "context", c. the success and active participation of the learner in his learning, the development of life skills as well as the development of procedural knowledge skills that were included in the category called "learning outcomes" and d. the modification of "learning" products, the alternative / modern forms of assessment and the continuous assessment that were dimensions of the category "assessment". The results of the research show that the dimension with the highest frequency is a modification of the supportive learning context and follows in order of frequency of occurrence, the modification of learning "products" and meeting the needs of learners. Finally, the dimensions with the lowest frequency of occurrence include the continuous assessment and the development of procedural knowledge skills.