NATURE OF VARIABILITY OF CANDAHARIA LEVANDERI (SIMROTH, 1902) IN THE FERGHANA AND SURKHAN - SHERABAD VALLEYS, UZBEKISTAN

The variability of Candaharia levanderi (Simroth, 1902)(Gastropoda, Stylommatophora, Parmacellidae) in two biotopes (southern and northern slopes, the Kampirtepa gorges, the Kugitang Tau ridge) has been investigated using polymerase chain reaction (PCR) with the implementation of primers, the 18S DNA of the region is amplified, the variability (sharply differing in color) of two populations of C. levanderi is studied. The first population is in the suburbs of Namangan, (Namangan Region); the second population is in Kampirtepa gorges, Kugitang Tau ridge (Surkhandarya Region). It is established that, most often, the variability of morphological signs is observed on the coloration of mollusks. The development of body coloration is an adaptive feature that reflects the adaptability to certain biotopes on the one hand, and landscape and climatic conditions on the other.

MOLECULAR CHARACTERIZATION OF ANISAKID NEMATODES HYSTEROTHYLACIUM SPECIES FROM JAPANESE THREADFIN BREAM NEMIPTERUS JAPONICUS (BLOCH, 1791) (PERCIFORMES, NEMIPERIDAE) FROM IRAQI MARINE WATER FISH

The present study provides a new insight into valuable information on the diverse structure of the Anisakid population and discusses the limited species richness in the Nemipterus japonicus (Bloch,1791) (Perciformes, Nemiperidae). The fishing area consists of various locations in the Arabian Gulf (29°58 0 33 00 N48°28 0 20 E). A total of 315 marine fish were examined, (n=287) were infected. Larval stages (n= 763) encysted within the mesenteries peritoneum and viscera of fish organs were isolated, with a prevalence of 91.11% of infection and, the intensity was 2.65. Molecular analysis was carried out on thirty individuals who have examined the morphology and showed some appearance differences, by amplifying internal transcribed spacers ITS and ITS-1 of nuclear rDNA (rDNA) by PCR using the primer sets NC5/NC2 and SS1/NC13R of thirteen DNA products. Evolutionary analyses were conducted in MEGA X. based on the identity percentage in the GenBank database showed that they belong to anisakid nematodes, in particular, they belong to eleven distinct taxa within the Hysterothylacium Ward & Magath, 1917 (Rhabditida, Raphidascarididae) and one identified species H. amoyense (Hsü, 1933) Deardorff & Overstreet, 1980. The current study records eleven species that belong to a genus of Hysterothylacium; some of the alignment of sequences polymorphisms reveals new different individuals of larvae species that may be adopted as new species if their adult stage is detected, and N. japonicus fish considered as a new host record. The current study provides some insights on the systematic taxonomy of these parasites, in addition, it supports similar studies that have been published elsewhere.

A MULTIVARIATE MORPHOMETRIC ANALYSIS OF THE GENUS LOTUS L., 1753 (FABACEAE, LOTEAE) FROM EGYPT

This study aims at examining and confirming the patterns of phenetic relationships and the levels of variations within and among the species of Lotus L., 1753 in Egypt by using morphometric analysis techniques. We have evaluated 24 morphological characters from about 300 herbarium specimens representing 19 species of Lotus that are currently recognized. Based on numerical analyses of macromorphological characters (cluster analysis, principal coordinate analysis and principal component analysis), 19 species of Lotus were recognized from Egypt. These species were clustered in six species-specific groups: (I) Lotus halophilus Boiss. & Spruner, L. angustissimus L., L. glinoides Delile and L. schimperi Steud. ex Boiss., (II) Lotus glaber Mill. and L. palustris Willd., (III) Lotus polyphyllos E.D. Clarke, L. creticus L. and L. cytisoides L., (IV) Lotus gebelia Vent., L. lanuginosus Vent. and L. arenarius Brot., (V) Lotus edulis L., L. tetragonolobus L. and L. conjugatus L. and (VI) Lotus ornithopodioides L., L. peregrinus L., L. arabicus L. and L. hebranicus Hochst. ex Brand. As a result of this study, we proposed that some characters, not previously examined in detail, showed significant characters in species delimitation: pod length, seed dimensions, features of upper and lower leaflets, calyx, length of corolla, length of style, numbers of flowers and ovules.

NEW RECORD OF THE LAND SNAIL POLYGYRA CEREOLUS (MEGERLE VON MÜHLFELD, 1818) (GASTROPODA, STYLOMMATOPHORA, POLYGYRIDAE) FOR MALACOFAUNA OF IRAQ

In this study, the specimens of land snails Polygyra cereolus (Megerle von Mühlfeldt, 1818) (Gastropoda, Stylommatophora, Polygyridae) are collected between March and April 2021 from gardens and nurseries in Baghdad province, this species was recorded as a new record to Iraq molluscan fauna. Description of the most important characteristics, measurements of the shell are presented with digital photographs, subsequently this study represents the first record of the Polygyridae in Iraq.

A SURVEY OF RUNNING CRAB SPIDERS PHILODROMIDAE (ARANEAE) OF ARMENIA

Twelve species of philodromid crab or running crab spiders (Philodromidae) have been recorded in Armenia. Nine species are new to the spider fauna of this country: Philodromus cespitum (Walckenaer, 1802); Philodromus emarginatus (Schrank, 1803), Philodromus rufus Walckenaer, 1826; Rhysodromus histrio (Latreille, 1819), Thanatus atratus Simon, 1875; Thanatus formicinus (Clerck, 1757); Thanatus imbecillus L. Koch, 1878; Thanatus vulgaris Simon, 1870 and Thanatus pictus L. Koch, 1881.

FIRST PHOTOGRAPHIC RECORDS AND NEW DISTRIBUTION RANGE OF THE ENDANGERED LONG-TAILED NESOKIA NESOKIA BUNNII (KHAJURIA, 1981)

In the 1970s, the world knew the long-tailed nesokia Nesokia bunnii (Khajuria, 1981) (Rodentia, Muridae) from the Mesopotamian marshes of Garden of Eden in Southern Iraq. This distinct rodent was known from only five voucher specimens collected at the confluence of Tigris and Euphrates Rivers in southern Iraq while its occurrence in Southwestern Iran had never been reported. In the 1990s, a large extent of its natural habitat was catastrophically desiccated and the animal was last seen in the 1970s. Since then, the status of this elusive rodent was shrouded in mystery. In 2007, an extraordinary photograph of a carcass of this species came to the light from Hawizeh Marsh which was interpreted as concrete evidence of the species’ persistence in the marshes of southern Iraq after the desiccation in the last century. In 2021, after more than 40 years, exclusive photographic records of living N. bunnii were obtained for the first time from Central Marshes in southern Iraq and from Edhe’am Marsh in southwestern Iran. The new distribution range is highlighted in this note. Furthermore, the first photographs of living N. bunnii are provided along with notes on its ecology and behavior.

NEW RECORD OF THE GENUS LARRA FABRICIUS, 1793 (HYMENOPTERA, CRABRONIDAE) FROM VIETNAM

The genus Larra Fabricius, 1793 (Hymenoptera: Crabronidae) is recorded for the first time from Vietnam. Three species and two subspecies belonging to this genus as follows: L. amplipennis (F. Smith, 1873); L. carbonaria (F. Smith, 1858); L. fenchihuensis Tsuneki, 1967; L. polita polita (F. Smith, 1858) and L. polita luzonensis Rohwer, 1919 are presented. Keys to both sexes of the three species and two subspecies reported here are provided.

NUMERICAL TAXONOMY OF GENUS FICUS L. 1753 (MORACEAE), WITH ADDITION NEW RECORD SPECIES TO EGYPT

The taxonomy of Ficus L., 1753 species is confusing because of the intense morphological variability and the ambiguity of the taxa. This study handled 36 macro-morphological characteristics to clarify the taxonomic identity of the taxa. The study revealed that Ficus is represented in the Egyptian gardens with forty-one taxa; 33 species, 4 subspecies and 4 varieties, and classified into five subgenera: Ficus Corner, 1960; Terega Raf., 1838; Sycomorus Raf., 1838; Synoecia (Miq.) Miq., 1867, and Spherosuke Raf.,1838; out of them seven were misidentified. Amongst, four new Ficus taxa were recently introduced to Egypt namely: F. lingua subsp. lingua Warb. ex De Wild. & T. Durand, 1901; F. pumila L., 1753; F. rumphii Blume, 1825, and F. sur Forssk., 1775. The application of the multivariate analyses in plant systematics namely the two-way clustering analysis and the principal component analysis revealed that the qualitative characters as the presence or absence of lateral peduncular or ostiolar bracts and the leaf margin delimit the differentiation of subgenera within genus Ficus. Whereas the qualitative characters of the leaf as leaf arrangement, lamina shape, length, ratio of length to width, base, apex, number of lateral veins, stipules and figs either pedunculate or sessile, shape, and width are significantly separating the species within the different sections. Seven different identification keys of the studied taxa based on the examined characters are provided. In addition, a diagrammatic key for all the studied taxa is given.

ACCUMULATION OF SOME HEAVY METALS IN LARVAE OF CONTRACAECUM SP. AND THEIR HOST TIGRIS CATFISH SILURUS TRIOSTEGUS HECKEL, 1843 IN BAGHDAD, IRAQ

This study was achieved to investigate the accumulation of some heavy metals included: Cadmium, Lead and Nickel in the tissues (gill, intestine, liver, muscles and skin) of Silurus triostegus Heckel, 1843 (Siluriformes, Siluridae) and its larval stage of the nematode Contracaecum sp. (Rhabditida, Anisakidae). As well as to assess the infection patterns of Contracaecum among S. triostegus specimens which were purchased fresh from the local market in Baghdad. One hundred and nine nematodes specimens in larval stage were recovered from the fish host; the overall prevalence of Contracaecum sp. was 38.6%. The sex of the host was not significantly (P ˃ 0.05) associated with the infection of this nematode. Results showed that the overall mean intensity of Contracaecum sp. was 6.41; mean intensity did not differ significantly (P ˃ 0.05) between the fish sexes.The lead (Pb) was the only element detected in all fish tissues investigated as well as in the parasite, while the cadmium and nickel elements were not detected in all specimens. Skin and muscles of the fish, as well the parasite Contracaecum sp. contained the lowest lead levels compared to other fish tissues (gill, intestine and liver), although no significant differences were noticed among all investigated tissues and the parasite regarding the concentration of Pb.

MORPHOLOGY AND MOLECULAR IDENTIFICATION OF THE LARVAL STAGE OF TWO SPECIES FROM THE GENUS CHRYSOBOTHRIS ESCHSCHOLTZ, 1829 (COLEOPTERA, BUPRESTIDAE)

The genus of Chrysobothris Eschscholtz, 1829 is one of the most diverse and widespread genera of the family Buprestidae of some 700 described species distributed throughout the world. In Iraq, particularly in the Kurdistan region, about 4 species had been recorded so far, many of these species are sympatric, share larval host plants, and are difficult to reliably separate morphologically. The current study investigates species limits and relationships among the recognized species occurring within the Erbil Province; mitochondrial cytochrome C oxidase (COX I) molecular analysis confirmed the monophyly of two Chrysobothris species, Ch. affinis (Fabricius, 1794) and Ch. chrysostigma (Linnaeus, 1758). Implications of the resultant larval morphology and molecular techniques are discussed. Diagnostic characteristics that are depended to identifying the species within Chrysobothris in larval stage were illustrated and then compared with the molecular data.