Tartary buckwheat grains were hydrothermally treated to establish the conditions under which rutin remains in the grain. Tartary buckwheat grains were soaked in water at the temperatures 51 °C, 61 °C, 70 °C, 75 °C, 80 °C, 85 °C, 90 °C, 93 °C, 97 °C and 99 °C, and a control group at 21 °C. During 20 minutes soaking at 51 °C or 61 °C the concentration of rutin decreased. This effect was mostly pronounced by soaking at 70 °C and 75 °C, where instead of missing amount of rutin, some quercetin appeared. After soaking at 80 °C, 85 °C, 90 °C, 93 °C, 97 °C and 99 °C concentration of rutin was not significantly different in comparison to the concentration of rutin after soaking 20 minutes at 21°C. It is suggested that exposure to water at 21°C is similar to natural conditions, where rutin degrading enzymes remain mainly inactive and in grain separated from its potential substrate. Further is suggested that at the soaking temperatures 51 °C, 61 °C, 70 °C and 75 °C, grain structures are partly degraded and rutin degrading enzymes got contact to the substrate. By soaking at 80 °C, 85 °C, 90 °C, 93 °C, 97 °C and 99 °C, rutin degrading enzymes lose their activity. Thus wet treatment of Tartary buckwheat grains for 20 minutes at temperature at 80 °C or above, this threshold is enough to preserve the content of rutin in the samples. This is of importance for nutritional quality of Tartary buckwheat food products.