The current exploration is being fetched out to evaluate the consequence of dairy effluent on morphological aspect and biochemical constituents in Moth bean (Vigna aconitifolia (Jacq.) Marechal). For this, experimentation work dairy effluents were amassed from the outlet of milk processing plants. The diverse concentrations used for the effluent were Control (normal water), 5, 25, 50, 75 and 100%. The growth parameters viz., Shoot Length (SL), Roots Length (RL), Number of Leaves (NL), Total Leaf Area (TLA), Fresh Weight (FW) and Dry Weight (DW), and the biochemical issue like viz., chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll, carotenoid, reducing, non-reducing sugar, total sugars, starch, amino acid, and protein content were analysed