In conventional transmission line protection, a distance relay is used to provide the primary as well as backup protection. The voltage and current phasors measurement needed by the distance relay for determining the impedance may be affected by the power disturbances such as power swing. Consequently, this power swing may cause mal-operation of Zone three distance relays which in turn may affect on the reliability of the whole protective scheme. Many power swing blocking functions (PSB) have been developed to mitigate these effects. In this paper, a new model of Mho distance relay with dual-quadrilateral power swing detection characteristic has been developed and implemented first in PC using LabVIEW, then tested using Power System Simulink Model under different faults and power swing conditions. Finally, the relay prototype has been realized using acquisition card NI USB-6009, which acquires real-time signals of the currents and the voltages, processes them digitally and outputs tripping or blocking signal to the circuit breaker. The obtained results show that the relay provides good discrimination between a fault and power swing condition.