Differential proteomic analysis of developmental stages of Acca sellowiana somatic embryos

2009 ◽  
Vol 31 (3) ◽  
pp. 501-514 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Andrea Villarino ◽  
Daniela Seixas ◽  
Eliane Dumas-Gaudot ◽  
Hernán Terenzi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Somatic Embryos ◽  
Developmental Stages ◽  
Acca Sellowiana

Comparative proteomic analysis of off-type and normal phenotype somatic plantlets derived from somatic embryos of Feijoa (Acca sellowiana (O. Berg) Burret)

Plant Science ◽  
2013 ◽  
Vol 210 ◽  
pp. 224-231 ◽  
Author(s):  
Hugo Pacheco de Freitas Fraga ◽  
Sarah Zanon Agapito-Tenfen ◽  
Clarissa Alves Caprestano ◽  
Rubens Onofre Nodari ◽  
Miguel Pedro Guerra
Keyword(s):  
Proteomic Analysis ◽  
Somatic Embryos ◽  
Normal Phenotype ◽  
Comparative Proteomic Analysis ◽  
Acca Sellowiana

scholarly journals Integrated transcriptomic and proteomic analysis reveals the complex molecular mechanisms underlying stone cell formation in Korla pear

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aisajan Mamat ◽  
Kuerban Tusong ◽  
Juan Xu ◽  
Peng Yan ◽  
Chuang Mei ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Cell Formation ◽  
Parenchyma Cell ◽  
Lignin Biosynthesis ◽  
Cell Content ◽  
Stone Cell ◽  
Fruit Samples

AbstractKorla pear (Pyrus sinkiangensis Yü) is a landrace selected from a hybrid pear species in the Xinjiang Autonomous Region in China. In recent years, pericarp roughening has been one of the major factors that adversely affects fruit quality. Compared with regular fruits, rough-skin fruits have a greater stone cell content. Stone cells compose sclerenchyma tissue that is formed by secondary thickening of parenchyma cell walls. In this work, we determined the main components of stone cells by isolating them from the pulp of rough-skin fruits at the ripening stage. Stone cell staining and apoptosis detection were then performed on fruit samples that were collected at three different developmental stages (20, 50 and 80 days after flowering (DAF)) representing the prime, late and stationary stages of stone cell differentiation, respectively. The same batches of samples were used for parallel transcriptomic and proteomic analysis to identify candidate genes and proteins that are related to SCW biogenesis in Korla pear fruits. The results showed that stone cells are mainly composed of cellulose (52%), hemicellulose (23%), lignin (20%) and a small amount of polysaccharides (3%). The periods of stone cell differentiation and cell apoptosis were synchronous and primarily occurred from 0 to 50 DAF. The stone cell components increased abundantly at 20 DAF but then decreased gradually. A total of 24,268 differentially expressed genes (DEGs) and 1011 differentially accumulated proteins (DAPs) were identified from the transcriptomic and proteomic data, respectively. We screened the DEGs and DAPs that were enriched in SCW-related pathways, including those associated with lignin biosynthesis (94 DEGs and 31 DAPs), cellulose and xylan biosynthesis (46 DEGs and 18 DAPs), S-adenosylmethionine (SAM) metabolic processes (10 DEGs and 3 DAPs), apoplastic ROS production (16 DEGs and 2 DAPs), and cell death (14 DEGs and 6 DAPs). Among the identified DEGs and DAPs, 63 significantly changed at both the transcript and protein levels during the experimental periods. In addition, the majority of these identified genes and proteins were expressed the most at the prime stage of stone cell differentiation, but their levels gradually decreased at the later stages.

Proteomic analysis and immunodetection of antigens from early developmental stages of Trichinella spiralis

2016 ◽  
Vol 231 ◽  
pp. 22-31 ◽  
Author(s):  
Rosa Ma. Bermúdez-Cruz ◽  
R. Fonseca–Liñán ◽  
Lucia Elhy Grijalva-Contreras ◽  
Guillermo Mendoza-Hernández ◽  
M. Guadalupe Ortega-Pierres
Keyword(s):  
Proteomic Analysis ◽  
Developmental Stages ◽  
Trichinella Spiralis ◽  
Early Developmental Stages ◽  
Early Developmental

scholarly journals Proteomic Analysis of Cell Walls of Two Developmental Stages of Alfalfa Stems

2012 ◽  
Vol 3 ◽  
Author(s):  
Julian C. Verdonk ◽  
Ronald D. Hatfield ◽  
Michael L. Sullivan
Keyword(s):  
Proteomic Analysis ◽  
Cell Walls ◽  
Developmental Stages

scholarly journals Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1486 ◽  
Author(s):  
Zhiyong Zhan ◽  
Yicun Chen ◽  
Jay Shockey ◽  
Xiaojiao Han ◽  
Yangdong Wang
Keyword(s):  
Proteomic Analysis ◽  
Developmental Stages ◽  
Tung Tree ◽  
Vernicia Fordii

scholarly journals Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina

2021 ◽  
Author(s):  
◽  
Ramavati Pal
Keyword(s):  
Insecticide Resistance ◽  
Proteomic Analysis ◽  
Developmental Stages ◽  
Adult Stage ◽  
Glutathione Transferases ◽  
Lucilia Cuprina ◽  
Main Body ◽  
Post Translational Modification ◽  
2D Page ◽  
Gst Activity

<p>The glutathione transferases are a family of multifunctional enzymes involved in detoxification of xenobiotic and endogenous electrophilic compounds. Interest in insect GSTs has primarily focused on their role in insecticide resistance. The sheep blowfly, Lucilia cuprina is a major economic problem for the sheep meat and wool industries in Australasia and hence this thesis has attempted the study of the Lucilia cuprina GST family, using proteomics, with a view to eventually determining their role in insecticide resistance. Combinations of different affinity matrices (glutathione-Sepharose matrix (GSH) followed by dinitrophenyl-glutathione-Sepharose matrix (DNP-GSH)) and two-dimensional electrophoresis has successfully isolated members from major four insect GST classes: Sigma, Delta, Epsilon and Omega. Drosophila melanogaster has been used as a model insect throughout as a basis for comparison. To characterise Lucilia GSTs, the whole metazoan fragmentation database was used for sequence alignment with Lucilia peptides. This approach is broad and speculative but predicts a possible classification of the GSTs based on % similarity and % identity. This method of characterisation yielded match scores that provided a basis for classification, which must at present be regarded as tentative and in need of confirmation. In D. melanogaster and L. cuprina, GSH affinity-purified extracts showed the presence of only Sigma and Delta GSTs. In D. melanogaster, the DNP-GSH affinity-purified GSTs showed mostly the presence of Epsilon and Omega GSTs whereas in L. cuprina no Omega GSTs were detected. In both species, the migration pattern of Delta GST on 2D PAGE gel indicated possible post-translational modification. The results from analysis of LC-MS/MS data by the software PEAKS suggested deamidation at asparagine and glutamine residues in a limited number of the matched peptides of Delta GST. GST activity was present in all developmental stages of L. cuprina. The number of isoenzymes and their extent of expression vary as the insect develops. Delta GSTs were present in all developmental stages. The Sigma GST started expressing from the larval stage and was abundantly present in adult stage. The DNP-GSH affinity matrix purified GSTs which have been tentatively classified as Mu-like GSTs were present in egg, larvae and pupae but totally absent in adult stage. The GST families were characterised by proteomics in the main body sections of L. cuprina. Higher GST activity towards 1-chloro-2, 4-dinitrobenzene (CDNB) was found in the thorax (65.2 %) followed by the abdomen (19.6%) and the head (15.2%). The cytosolic GSTs of a resistant strain (PY81) of L. cuprina had significantly higher (2.26- and 2.6- fold) activity than the susceptible strains (NSW and CSIRO) towards CDNB and 2, 3-dichloro, 4-nitrobenzene (DCNB) respectively. The proteomic analysis of DNP-GSH purified extract from susceptible and resistant strains showed quantitatively higher expression of GSTs on 2D PAGE gel of the PY81 strain. The in vitro interaction of purified GSTs and model insecticides studied by high performance liquid chromatography revealed that Delta and DNP-GSH affinity-purified GSTs catalyse the conjugation of the insecticides to reduced glutathione but Sigma GST had almost no activity.</p>

scholarly journals Somatic Embryogenesis in Centella asiatica, a Valuable Medicinal Plant

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 859C-859
Author(s):  
Nirmal Joshee* ◽  
Bipul K. Biswas ◽  
Anand K. Yadav
Keyword(s):  
Somatic Embryos ◽  
Bacterial Contamination ◽  
Developmental Stages ◽  
Basal Medium ◽  
Centella Asiatica ◽  
Perennial Herb ◽  
Culture Techniques ◽  
Nodular Callus ◽  
Further Development

Centella asiatica L. (Apiaceae family), also called `Indian Pennywort,' is a prostrate, faintly aromatic, and stoloniferous perennial herb with long petiolated leaves. In the Ayurvedic medicine, it is reputed as a nervine tonic along with antibacterial, antifeedant, antileprotic and wound healing properties. Centella contains glycosides, indocentelloside, brahmoside, and asiaticoside. Its leaves are rich in carotenoids and vitamins B and C. In vitro culture techniques which offer a viable tool for mass propagation of plants have recently become increasingly popular for conservation of rare, endangered and threatened medicinal plants germplasm. Centella tissue culture has been reported to experience high incidences of microbial contamination which drastically reduces survival of explants. Thus, the main purpose of this study was to develop an efficient micropropagation technique for Centella asiatica to reduce explant contamination and rapidly disseminate superior clones for research and production. Here we present induction and further development of somatic embryos, using Centella stolons as explants. Somatic embryos were induced in response to 2,4-D shock on MS medium. Initially, somatic embryos appeared as highly nodular callus and eventually developed into somatic embryos that exhibited globular, heart shaped and cotyledonary stages. After auxin shock, cultures were regularly transferred to MS basal medium where somatic embryos completed various developmental stages and then germinated to give rise to new plantlets. In this presentation, we will demonstrate complete protocols for the successful sterilization of Centella explants prepared from plants that had abundance of fungal and bacterial contamination.

scholarly journals MORPHOLOGICAL CHANGES DURING THE DEVELOPMENT OF SOMATIC EMBRYOS OF SAGO (Metroxylon sagu Rottb.)

2016 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Pauline D. Kasi ◽  
Sumaryono Sumaryono
Keyword(s):  
Developmental Stage ◽  
Somatic Embryos ◽  
Large Scale ◽  
Developmental Stages ◽  
Morphological Changes ◽  
Petri Dish ◽  
Synthetic Seed ◽  
Globular Embryos ◽  
Metroxylon Sagu

Development of somatic embryos of sago (Metroxylon sagu Rottb.) on agar-solidified medium are highly varied producing heterogeneous seedlings. Understanding of this phenomenon may help in improving the cultural procedures and conditions of sago<br />somatic embryogenesis to obtain uniform seedlings in a large scale. This experiment was conducted at the laboratory for plant cell culture and micropropagation, Indonesian Biotechnology Research Institute for Estate Crops from January to March 2006 to examine morphological changes i.e. color and development stages of sago during their somatic embryo development on an agar-solidified medium. Twenty single globular somatic embryos of sago with specific color (yellowish, greenish, and reddish) were cultured in a Petri dish supplemented with a solid medium. The medium was a micronutrients-modified MS (MMS) with half strength of macronutrients containing 0.01 mg l-1 ABA, 2 mg l-1 kinetin, 20 g l-1 sucrose, 0.5 g l-1 activated charcoal, and 2 g l-1 gelrite. Parameter observed was the percentage of embryo’s number based on color and developmental stage. The result showed that at the end of 6-week culture passage, most originally greenish (80.8%) and reddish (95.8%) embryos remained unchanged in their colors, whereas almost half of the originally yellowish embryos turned to greenish and only 30%<br />remained yellowish. At the same time, single globular embryos have changed gradually into the next developmental stages, although not all of the embryos were germinated. The initial color of embryo affected the rate of the developmental stage changes. Yellowish and greenish globular embryos developed more rapidly into cotyledon or germinant stages at 58% and 55% respectively, in 6 weeks than the reddish ones (41%). Therefore, the yellowish and greenish embryos are the best sources of material for in vitro mass propagation and synthetic seed production of sago.

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