Development of a cost-effective medium for submerged production of fungal aryl alcohol oxidase using a genetically modified Aspergillus nidulans strain

2020 ◽  
Vol 305 ◽  
pp. 123038 ◽  
Author(s):  
Enshi Liu ◽  
Mengxing Li ◽  
Asmaa Abdella ◽  
Mark R. Wilkins
Keyword(s):  
Aspergillus Nidulans ◽  
Genetically Modified ◽  
Alcohol Oxidase ◽  
Cost Effective ◽  
Effective Medium

Qualitative and Quantitative PCR-Based Detection Methods for Authorized Genetically Modified Cotton Events in India

2014 ◽  
Vol 97 (5) ◽  
pp. 1299-1309 ◽  
Author(s):  
Rashmi Chhabra ◽  
Gurinder Jit Randhawa ◽  
Rajesh K Bhoge ◽  
Monika Singh
Keyword(s):  
Genetically Modified ◽  
Cost Effective ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Bt Cotton ◽  
Pcr Assay ◽  
Genetically Modified Cotton ◽  
Detection Assay ◽  
Endogenous Reference ◽  
The Cost

Abstract Qualitative diagnostics for all five commercialized genetically modified (GM) cotton events for insect resistance in India is being reported for the first time in this paper. The cost-effective and robust multiplex PCR (MPCR)-based detection assay, distinguishing the insect resistant transgenic Bt cotton events, viz., MON531, MON15985, Event 1, GFM-cry1A, and MLS-9124, has been developed. This decaplex PCR assay targets nine transgenic elements, viz., sequences of four transgenes, three transgene constructs, and two event-specific sequences along with one endogenous reference gene. The LOD of the qualitative MPCR assay was up to 0.1%. A quantitative detection method for four widely commercially cultivated GM cotton events, namely, MON531, MON15985, Event 1, and GFM-cry1A, covering 99.5% of the total area under GM cultivation in the country, is also reported. A construct-specific real-time PCR assay has been developed for quantification of these GM cotton events with LOQ <0.05% and LOD <0.025%. The developed assays will be of great use to screen for the presence/absence of authorized GM cotton events in unknown samples and to check the authenticity of GM cotton seed samples.

scholarly journals Development of a cost-effective medium for Photorhabdus temperata bioinsecticide production from wastewater and exploration of performance kinetic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sahar Keskes ◽  
Wafa Jallouli ◽  
Imen Ben Atitallah ◽  
Fatma Driss ◽  
Emna Sahli ◽  
...  
Keyword(s):  
Biomass Production ◽  
Chloride Concentration ◽  
Cost Effective ◽  
Culture Conditions ◽  
Effective Medium ◽  
Inoculum Size ◽  
Operating Conditions ◽  
Optimum Operating ◽  
Optimized Conditions ◽  
Photorhabdus Temperata

AbstractThis study investigates the optimization of the culture conditions for enhancing Photorhabdus temperata biopesticide production using wastewater (WS4) as a raw material. Box-Behnken design (BBD) was used to evaluate the effects of carbon to nitrogen ratio (C/N), sodium chloride concentration and inoculum size on P. temperata biomass production and insecticidal activity. For an enhanced biopesticide production, the optimum operating conditions were as follows: inoculum size = 4%; C/N ratio = 12.5 and [NaCl] = 4 g/L for two responses. 1.95 and 2.75 fold improvements in oral toxicity and biomass production were respectively obtained in the cost-effective medium developed in this study (WS4 I) using the three variables at their optimal values. Under the optimized conditions, WS4 I-grown cells exhibited higher membrane integrity according to flow cytometry analysis since dead cells presented only 9.2% compared to 29.2% in WS4. From batch fermentations carried out in WS4 I and WS4, P. temperata kinetic parameters in terms of biomass production and substrate consumption rates were modeled. The obtained results showed that the maximum specific growth rate in WS4 I was of 0.43 h−1 while that obtained in WS4 was of 0.14 h−1. In addition, the efficiency of P. temperata to metabolize organic carbon was enhanced by optimizing the culture conditions. It reached 72.66% instead of 46.18% in the control fermentation after 10 h of incubation. Under the optimized conditions, P. temperata cells showed the highest specific consumption rate resulting in a toxin synthesis improvement.

scholarly journals Aspergillus nidulans: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industrial Applications

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Amit Kumar
Keyword(s):  
Aspergillus Nidulans ◽  
Low Cost ◽  
Model Organism ◽  
Alcohol Oxidase ◽  
Industrial Applications ◽  
Genetically Engineered ◽  
Cell Factory ◽  
Industrial Enzymes ◽  
Fungal Cell ◽  
Potential Resource

Aspergillus nidulans is a filamentous fungus that is a potential resource for industrial enzymes. It is a versatile fungal cell factory that can synthesize various industrial enzymes such as cellulases, β-glucosidases, hemicellulases, laccases, lipases, proteases, β-galactosidases, tannases, keratinase, cutinases, and aryl alcohol oxidase. A. nidulans has shown the potential to utilize low-cost substrates such as wheat bran, rice straw, sugarcane bagasse, rice bran, coir pith, black gram residue, and chicken feathers to produce enzymes cost-effectively. A. nidulans has also been known as a model organism for the production of heterologous enzymes. Several studies reported genetically engineered strains of A. nidulans for the production of different enzymes. Native as well as heterologous enzymes of A. nidulans have been employed for various industrial processes.

scholarly journals Development of a Cost-effective Medium for Photorhabdus Temperata Bioinsecticide Production From Wastewater and Exploration of Performance Kinetic

2020 ◽  
Author(s):  
Sahar Keskes ◽  
Wafa Jallouli ◽  
Imen Ben Atitallah ◽  
Mohamed Chamkha ◽  
Slim Tounsi
Keyword(s):  
Biomass Production ◽  
Chloride Concentration ◽  
Cost Effective ◽  
Culture Conditions ◽  
Effective Medium ◽  
Inoculum Size ◽  
Raw Material ◽  
Oral Toxicity ◽  
Operation Conditions ◽  
Photorhabdus Temperata

Abstract This study investigates the optimization of culture conditions for enhancing Photorhabdus temperata biopesticide production using wastewater (WS4) as a raw material. Box-Behnken design (BBD) was used to evaluate the effect of carbon to nitrogen ratio (C/N), NaCl concentration and inoculum size on Photorhabdus temperata biomass production and insecticidal activity. Modelling results suggest that the selected variables had contributed significantly to the responses. For enhanced biopesticide production the optimum operation conditions were as follow: inoculum size=4 %; C/N ratio=12.5 and sodium chloride concentration= 4 g/L for two responses. 1.95 and 2.75 folds improvement in oral toxicity and biomass production, respectively, were obtained when using the three variables at their optimum values. From batch fermentations carried out in the cost-effective medium developed in this study (WS4 I) and WS4 used as control, P. temperata kinetic parameters in term of biomass production and substrate consumption were modeled. The obtained results showed that the maximum specific growth rate was of 0.38 h-1 compared to 0.16 h-1 obtained in WS4. In addition, the efficiency of P. temperata to metabolize organic carbon was enhanced by optimizing culture conditions reaching 70 % instead of 47.2 % in the control fermentation. Under the optimized conditions, P. temperata cells showed higher specific consumption rate resulting in P. temperata toxin synthesis improvement.

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