scholarly journals Biodegradation of Indanthrene Blue RS dye in immobilized continuous upflow packed bed bioreactor using corncob biochar

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Swati Sambita Mohanty ◽  
Arvind Kumar
Keyword(s):  
Removal Efficiency ◽  
Loading Rate ◽  
Textile Wastewater ◽  
Dye Decolorization ◽  
Packed Bed ◽  
Aerobic Biodegradation ◽  
Packed Bed Bioreactor ◽  
Initial Substrate ◽  
Decolorization Efficiency ◽  
Inoculum Volume

AbstractThe current study describes the aerobic biodegradation of Indanthrene Blue RS dye by a microbial consortium immobilized on corn-cob biochar in a continuous up-flow packed bed bioreactor. The adsorption experiments were performed without microbes to monitor the adsorption effects on initial dye decolorization efficiency. The batch experiments were carried out to estimate the process parameters, and the optimal values of pH, temperature, and inoculum volume were identified as 10.0, 30 °C, and 3.0 × 106 CFU mL−1, respectively. During the continuous operation, the effect of flow rate, initial substrate concentration, inlet loading rate of Indanthrene Blue RS on the elimination capacity, and its removal efficiency in the bioreactor was studied. The continuous up-flow packed bed bioreactor was performed at different flow rates (0.25 to 1.25 L h−1) under the optimal parameters. The maximum removal efficiency of 90% was observed, with the loading rate varying between 100 and 300 mg L−1 day−1. The up-flow packed bed bioreactor used for this study was extremely useful in eliminating Indanthrene Blue RS dye using both the biosorption and biodegradation process. Therefore, it is a potential treatment strategy for detoxifying textile wastewater containing anthraquinone-based dyes.

Biosorption and Biodegradation of Anthraquinone Based Dye (Indanthrene Blue RS) by the Immobilized Microbial Consortium in a Continuous Packed bed Bioreactor Using Corn-Cob Biochar

2021 ◽  
Author(s):  
Swati Sambita Mohanty ◽  
Arvind Kumar
Keyword(s):  
Removal Efficiency ◽  
Loading Rate ◽  
Microbial Consortium ◽  
Textile Wastewater ◽  
Dye Decolorization ◽  
Packed Bed ◽  
Aerobic Biodegradation ◽  
Corn Cob ◽  
Packed Bed Bioreactor ◽  
Inoculum Volume

Abstract The current study describes the aerobic biodegradation of Indanthrene Blue RS dye by a microbial consortium immobilized on corn-cob biochar in a continuous up-flow packed bed bioreactor. The adsorption experiments were performed without microbes to monitor the adsorption effects on initial dye decolorization efficiency. The batch experiments were carried out to estimate the process parameters, and the optimal values of pH, temperature, and inoculum volume were identified to be 10.0, 30 ºC, and 3.0 × 106 CFU mL-1, respectively. During the continuous operation, the effect of flow rate, initial substrate concentration, inlet loading rate of Indanthrene Blue RS on the elimination capacity, and its removal efficiency in the bioreactor was studied. The continuous up-flow packed bed bioreactor was performed at different flow rates (0.25 to 1.25 L h-1) under the optimal parameters. The maximum removal efficiency of 90% was observed, with the loading rate varying between 100 to 300 mg L-1 d-1. The up-flow packed bed bioreactor used for this study was extremely useful in eliminating Indanthrene Blue RS dye using both the biosorption and biodegradation process. Therefore, it is a potential treatment strategy for detoxifying textile wastewater containing anthraquinone based dyes.

scholarly journals Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 743
Author(s):  
Marcela Levio-Raiman ◽  
Gabriela Briceño ◽  
Bárbara Leiva ◽  
Sebastián López ◽  
Heidi Schalchli ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Alginate Bead ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Continuous Treatment ◽  
Packed Bed Bioreactor ◽  
Inoculum Concentration ◽  
Pesticide Removal ◽  
Pesticide Metabolites ◽  
Fungal Consortium

This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).

scholarly journals 4-chlorophenol removal by air lift packed bed bioreactor and its modeling by kinetics and numerical model (artificial neural network)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elahe Azizi ◽  
Fariba Abbasi ◽  
Mohammad Ali Baghapour ◽  
Mohammad Reza Shirdareh ◽  
Mohammad Reza Shooshtarian
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Removal Efficiency ◽  
Packed Bed ◽  
Packed Bed Bioreactor ◽  
First Order ◽  
Artificial Neural ◽  
High Concentrations ◽  
Artificial Neural Network Ann ◽  
Air Lift

Abstract4-chlorophenol (4-CP) is a hazardous contaminant that is hardly removed by some technologies. This study investigated the biodegradation, and physical 4-CP removal by a mixed microbial consortium in the Airlift packed bed bioreactor (ALPBB) and modeling by an artificial neural network (ANN) for first the time. The removal efficiency of ALPBB was investigated at 4-CP(1-1000 mg/L) and hydraulic retention time (HRT)(6-96 hr) by HPLC. The results showed that removal efficiency decreased from 85 at 1 to 0.03% at 1000 mg/L, with increasing 4-CP concentration and HRT decreasing. BOD5/COD increased with increasing exposure time and concentration decreasing, from 0.05 at 1000 to 0.96 at 1 mg/L. With time increasing, the correlation between COD and 4-CP removal increased (R2 = 0.5, HRT = 96 h). There was a positive correlation between the removal of 4-CP and SCOD by curve fitting was R2 = 0.93 and 0.96, respectively. Moreover, the kinetics of 4-CP removal follows the first-order and pseudo-first-order equation at 1 mg/L and other concentrations, respectively. 4-CP removal modeling has shown that the 2:3:1 and 2:4:1 were the best structures (MSE: physical = 0.126 and biological = 0.9)(R2allphysical = 0.999 and R2testphysical = 0.999) and (R2allbiological = 0.71, and R2testbiological = 0.997) for 4-CP removal. Also, the output obtained by the ANN prediction of 4-CP was correlated to the actual data (R2physical = 0.9997 and R2biological = 0.59). Based on the results, ALPBB with up-flow submerged aeration is a suitable option for the lower concentration of 4-CP, but it had less efficiency at high concentrations. So, physical removal of 4-CP was predominant in biological treatment. Therefore, the modification of this reactor for 4-CP removal is suggested at high concentrations.

Decolorization of Textile Wastewater Using Modified Facultative Anaerobic Baffled Reactor

2012 ◽  
Vol 627 ◽  
pp. 386-389
Author(s):  
Zheng Qin Liu ◽  
Lei Tan ◽  
Yue Li ◽  
Dan Li Xi
Keyword(s):  
Wastewater Treatment ◽  
Removal Efficiency ◽  
Textile Wastewater ◽  
Treatment System ◽  
Biological Degradation ◽  
Anaerobic Conditions ◽  
Anaerobic Baffled Reactor ◽  
Decolorization Efficiency ◽  
Facultative Anaerobic ◽  
Commercially Important

The study deals with a wastewater treatment system which is combination of modified facultative anaerobic baffled reactor (MFABR) and an aerobic unit. The efficient decolorization and biological degradation of the wastewater that contains commercially important cationic red 2GL (azo) in the MFABR under facultative anaerobic conditions are the research focuses. The results indicate that over 95.2% decolorization and about 54.6% COD removal efficiency can be obtained in MFABR. High dye concentration (500 mg/L) still results in over 90.6% decolorization efficiency. The main characteristics of the process are highly efficient decolorization in MFABR and greatly reducing COD in aerobic unit.

scholarly journals EFFECIENCY OF IMMOBILIZED POLYPHENOL OXIDASE ON SOME TEXTILE DYES DEGRADATION USING BATCH OPERATION SYSTEM BY PACKED BED BIOREACTOR

2019 ◽  
Vol 50 (3) ◽  
Author(s):  
S. I. Hussein
Keyword(s):  
Solanum Lycopersicum ◽  
Polyphenol Oxidase ◽  
Removal Efficiency ◽  
Gel Filtration ◽  
Packed Bed ◽  
Textile Dyes ◽  
Textile Dye ◽  
Operation System ◽  
Packed Bed Bioreactor ◽  
Batch Operation

In the current study, three types of common plants, namely Tomato (Solanum lycopersicum), Cucumber (Cucumis sativus) and Orange (Citrus sinensis) were obtained and screened for their polyphenol oxidase (PPO) activity, Among the three plants, Solanum lycopersicum was chosen with maximum enzymatic activity, it had the highest productivity of the enzyme (23733 U/mg protein).The PPO from Solanum lycopersicum was purified using two steps: concentration by sucrose and gel filtration by using Sephacryl S-200. The results showed an increase in the final purification folds by 2.4 times with an enzyme yield of 32.6%. The immobilization studies showed that PPO was more stable when immobilized on chitosan by covalent linkage with immobilization ratio of 62%, in comparison with agar-agar by entrapment method (36%). The removal efficiency of crude and partial purified PPO was studied with textile dyes, including yellow, red, black and blue dyes at optimum conditions: pH 5, temperature 40oC after 3 hrs. Maximum removal efficiency of dyes observed with crude PPO were 53.9, 81.4, 86.5 and 79.6% respectively. However, purified PPO displayed removal efficiency reached 60.3, 84.3, 84.6 and 77.5% respectively. The potential of immobilized PPO on chitosan was evaluated by decolorization of black textile dye in packed bed bioreactor in batch operation. The results indicated that immobilized PPO in batch operation has the ability to remove 99% of the dye after 2 hrs, and the results showed a positive relationship between the degradation rate and incubation time in batch operation.

Nitrate and phosphate removal in sulphur-coral stone autotrophic denitrification packed-bed reactorsA paper submitted to the Journal of Environmental Engineering and Science.

2009 ◽  
Vol 36 (5) ◽  
pp. 923-932 ◽  
Author(s):  
Yun-Jie Ruan ◽  
Guo-Zhi Luo ◽  
Hong-Xin Tan ◽  
Xuan Che ◽  
Yan Jiang ◽  
...  
Keyword(s):  
Water Temperature ◽  
Removal Efficiency ◽  
Loading Rate ◽  
Removal Rate ◽  
Packed Bed ◽  
Pilot Scale ◽  
Phosphate Removal ◽  
Environmental Engineering ◽  
Nitrite Accumulation ◽  
Autotrophic Denitrification

The removal effect on nitrate and phosphate of a sulphur-based autotrophic denitrifying bioreactor in composite packing of sulphur with coral stone were investigated on a pilot scale. The results showed that the optimal influent loading rate of the bioreactor (the ratio of sulphur volume to coral stone volume was 1:1) was in the range of 0.080–0.244 kg NO3–-N/(m3·d) when the water temperature was 29 ± 1 °C. In this range, the removal efficiency of nitrates was higher than 95% and no nitrite accumulation occurred in the effluent. The maximum nitrate volumetric removal rate of 0.594 kg NO3–-N / (m3·d) was achieved at an influent loading rate of 0.716 kg NO3–-N/(m3·d) with obvious nitrite accumulation in the effluent. Along with the autotrophic denitrifying reaction, an influent loading rate between 0.070–0.210 kg PO43–-P/ (m3·d) achieved the removal efficiency and volumetric removal rate of phosphates were 50.5%–89.2% and 0.045–0.179 kg PO43–-P/(m3·d), respectively. The phosphates related to the resistance ability of the bioreactor (the ratio of sulphur volume to coral stone volume was 1:1) was stronger than that of bioreactors (the ratio of sulphur volume to coral stone volume was 1:2 and 1:4).

Treatment of landfill leachate by a pilot-scale modified Ludzack-Ettinger and sulfur-utilizing denitrification process

2004 ◽  
Vol 50 (6) ◽  
pp. 141-148
Author(s):  
J.-H. Bae ◽  
I.-S. Lee ◽  
M.-S. Jang ◽  
K.-H. Ahn ◽  
S.-H. Lee
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Landfill Leachate ◽  
Loading Rate ◽  
Removal Rate ◽  
Packed Bed ◽  
Pilot Scale ◽  
Packed Bed Reactor ◽  
Treatment System ◽  
Flow Mode

Nitrogen removal efficiency of a pilot-scale system consisted of Modified Ludzack-Ettinger (MLE) followed by sulfur-utilizing denitrification (SUDNR) process was evaluated with a landfill leachate. For SUDNR, a down-flow mode sulfur packed bed reactor (SPBR) filled with sulfur and limestone particles was used. Although total nitrogen removal efficiency of the MLE process was about 80% at the recycle ratio of 4, effluent contained 350-450 mg/L NO3--N. Up to a loading rate of 1.2 kg NO3--N/m3-day, the SPBR could achieve complete removal of nitrate, and nitrate removal rate was kept to that level even at higher loading rate. When a COD/N ratio of MLE process was maintained at 2 instead of 4, more organics with molecular weight less than 500 were utilized for heterotrophic denitrification although denitrification was not complete with the lack of electron donors. Clogging in the SPBR, mainly by the accumulation of nitrogen gas in the pores, could easily be removed by introducing the effluent in an upward direction for 1 min at 1 hr intervals. The proposed treatment system could achieve nitrate free effluent with a slight increase in chemical cost. Furthermore, depending on further COD removal requirement after biological treatment, the proposed treatment system can be an economical solution.

Anaerobic Treatment of Polyethylene Terephthalate (PET) Wastewater from Lab to Full Scale

1999 ◽  
Vol 40 (8) ◽  
pp. 229-236 ◽  
Author(s):  
F. Fdz-Polanco ◽  
M. D. Hidalgo ◽  
M. Fdz-Polanco ◽  
P. A. García Encina
Keyword(s):  
Polyethylene Terephthalate ◽  
Loading Rate ◽  
Treatment Plant ◽  
Textile Wastewater ◽  
Anaerobic Treatment ◽  
Cod Removal ◽  
Organic Loading Rate ◽  
Foam Formation ◽  
Organic Loading ◽  
Start Up

In the last decade Polyethylene Terephthalate (PET) production is growing. The wastewater of the “Catalana de Polimers” factory in Barcelona (Spain) has two main streams of similar flow rate, esterification (COD=30,000 mg/l) and textile (COD=4000 mg/l). In order to assess the anaerobic treatment viability, discontinuous and continuous experiments were carried out. Discontinuous biodegradability tests indicated that anaerobic biodegradability was 90 and 75% for esterification and textile wastewater. The textile stream revealed some tendency to foam formation and inhibitory effects. Nutrients, micronutrients and alkali limitations and dosage were determined. A continuous lab-scale UASB reactor was able to treat a mixture of 50% (v) esterification/textile wastewater with stable behaviour at organic loading rate larger than 12 g COD/l.d (0.3 g COD/g VSS.d) with COD removal efficiency greater than 90%. The start-up period was very short and the recuperation after overloading accidents was quite fast, in spite of the wash-out of solids. From the laboratory information an industrial treatment plant was designed and built, during the start-up period COD removal efficiencies larger than 90% and organic loading rate of 0.6 kg COD/kg VSS.d (5 kg COD/m3.d) have been reached.

Three-dimensional CFD simulation of anaerobic reactions in a continuous packed-bed bioreactor

2021 ◽  
Author(s):  
Sasan Zarei ◽  
Seyyed Mohammad Mousavi ◽  
Teimour Amani ◽  
Mehrdad Khamforoush ◽  
Arezou Jafari
Keyword(s):  
Cfd Simulation ◽  
Three Dimensional ◽  
Packed Bed ◽  
Packed Bed Bioreactor

Toxicity and recalcitrant compound removal from bleaching pulp plant effluents by an integrated system: anaerobic packed-bed bioreactor and ozone

2010 ◽  
Vol 61 (1) ◽  
pp. 199-205 ◽  
Author(s):  
T. R. Chaparro ◽  
C. M. Botta ◽  
E. C. Pires
Keyword(s):  
Pulp Mill ◽  
Packed Bed ◽  
Ultraviolet Absorption ◽  
Integrated System ◽  
Packed Bed Bioreactor ◽  
Cellulose Pulp ◽  
Adsorbable Organic Halides ◽  
Organic Halides ◽  
Before And After ◽  
Absorption Measurements

Effluents originated in cellulose pulp manufacturing processes are usually toxic and recalcitrant, specially the bleaching effluents, which exhibit high contents of aromatic compounds (e.g. residual lignin derivates). Although biological processes are normally used, their efficiency for the removal of toxic lignin derivates is low. The toxicity and recalcitrance of a bleached Kraft pulp mill were assessed through bioassays and ultraviolet absorption measurements, i.e. acid soluble lignin (ASL), UV280, and specific ultraviolet absorption (SUVA), before and after treatment by an integrated system comprised of an anaerobic packed-bed bioreactor and oxidation step with ozone. Furthermore, adsorbable organic halides (AOX) were measured. The results demonstrated not only that the toxic recalcitrant compounds can be removed successfully using integrated system, but also the ultraviolet absorption measurements can be an interesting control-parameter in a wastewater treatment.

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