Automated in situ preparation of azomethine H and the subsequent determination of boron in aqueous solution

CdS, a bright yellow precipitate is well known for its optical, electrical, electro-optical and photochemical properties. CdS has also been prepared in the interlayer spaces of layered materials such as laponite and montmorillonite (bentonite). Several properties of intercalated CdS have been studied, but for the best of our knowledge there is no reports on its use. In this work, we applied CdS-intercalated bentonite / carbon composite as an electrode for determination of S2- ion in aqueous solution. CdS- intercalated bentonite was prepared by the in situ solid-solid reaction between Cd(II)-bentonite and Na2S. To construct a potentiometric electrode, CdS-intercalated bentonite was mixed with artificial graphite, polytetrafluoroethylene (PTFE) and carboxymethylcellulose (CMC) in an appropriate ratio. The electrode was used to determine S2- in aqueous solution, graphs of log [S2-] vs measured voltage (mv) gave linear relation up to 3 orders of ion concentrations. Apart from the reproducibility and good precision, the electrode is good for its selectivity, durability and low cost.

Download Full-text

Genome multiplication is a generalised phenomenon in placentomal and interplacentomal trophoblast giant cells in cattle

Reproduction Fertility and Development ◽

10.1071/rd03101 ◽

2004 ◽

Vol 16 (3) ◽

pp. 301 ◽

Cited By ~ 7

Author(s):

Karl Klisch ◽

Preben D. Thomsen ◽

Vibeke Dantzer ◽

Rudolf Leiser

Keyword(s):

Dna Content ◽

Nuclear Dna ◽

In Situ Hybridisation ◽

Nuclear Dna Content ◽

Giant Cells ◽

Crown Rump Length ◽

Subsequent Determination ◽

Trophoblast Giant Cells

The frequency of polyploidisation in bovine binucleate trophoblast giant cells (TGC) from placentomes (PL) and the interplacentomal allantochorion (AL) of six male fetuses with a crown–rump length between 3.5 and 103 cm was determined by in situ hybridisation with a chromosome-7-specific probe, using a probe specific for the Y chromosome to distinguish between maternal and fetal nuclei. The results showed that polyploid nuclei were essentially always of fetal origin. The frequency of tetraploid nuclei varied between 3% and 15% in both the placentomal and interplacentomal samples, with mean frequencies of 8.8% and 10.0% respectively. Octoploid nuclei were observed with a mean frequency of 1.1% in the interplacentomal samples, but were absent in samples from placentomes. Subsequent determination of nuclear DNA content by cytophotometric measurement of Feulgen-stained nuclei revealed that the frequency of nuclei with an 8C DNA content was several fold higher (AL 5.4%; PL 7.8%) than the frequency of octoploidy, suggesting that tetraploid TGC cells are arrested in the G2 phase of the cell cycle.

Download Full-text

In situ preparation and fate of cis-4-hydroxycyclophosphamide and aldophosphamide: proton and phosphorus-31 NMR evidence for equilibration of cis- and trans-4-hydroxycyclophosphamide with aldophosphamide and its hydrate in aqueous solution

Journal of Medicinal Chemistry ◽

10.1021/jm00370a010 ◽

1984 ◽

Vol 27 (4) ◽

pp. 490-494 ◽

Cited By ~ 22

Author(s):

Richard F. Borch ◽

Thomas R. Hoye ◽

Todd A. Swanson

Keyword(s):

Aqueous Solution ◽

In Situ Preparation ◽

Cis And Trans

Download Full-text