scholarly journals Inverse electron demand Diels–Alder (iEDDA)-initiated conjugation: a (high) potential click chemistry scheme

2013 ◽  
Vol 42 (12) ◽  
pp. 5131 ◽  
Author(s):  
Astrid-Caroline Knall ◽  
Christian Slugovc
2016 ◽  
Vol 52 (53) ◽  
pp. 8267-8270 ◽  
Author(s):  
Daniel A. Lorenz ◽  
Amanda L. Garner

A catalytic enzyme-linked click chemistry assay (cat-ELCCA) for Dicer-catalyzed pre-microRNA maturation was optimized to employ inverse-electron demand Diels–Alder (IEDDA) chemistry affording high-throughput screening capability.


2011 ◽  
Vol 22 (10) ◽  
pp. 2048-2059 ◽  
Author(s):  
Brian M. Zeglis ◽  
Priya Mohindra ◽  
Gabriel I. Weissmann ◽  
Vadim Divilov ◽  
Scott A. Hilderbrand ◽  
...  

ChemistryOpen ◽  
2017 ◽  
Vol 6 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Christian Brand ◽  
Pasquale Iacono ◽  
Carlos Pérez-Medina ◽  
Willem J. M. Mulder ◽  
Moritz F. Kircher ◽  
...  

2019 ◽  
Author(s):  
Mathis Baalmann ◽  
Laura Neises ◽  
Sebastian Bitsch ◽  
Hendrik Schneider ◽  
Lukas Deweid ◽  
...  

A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA<sub>inv</sub>). With the aim of precisely generating branched protein chimeras, we systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. We demonstrate the efficiency and versatility of our conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technology enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines.


2016 ◽  
Vol 16 (1) ◽  
pp. 124-133 ◽  
Author(s):  
Jacob L. Houghton ◽  
Rosemery Membreno ◽  
Dalya Abdel-Atti ◽  
Kristen M. Cunanan ◽  
Sean Carlin ◽  
...  

2021 ◽  
Author(s):  
Jin Teh ◽  
Marta Costa-Braga ◽  
Louis Allott ◽  
Chris Barnes ◽  
Javier Hernandez-Gil ◽  
...  

The production of 18F-labelled microbubbles (MBs) via the aluminium-[18F]fluoride ([18F]AlF) radiolabelling method and facile inverse-electron-demand Diels-Alder (IEDDA) ‘click’ chemistry is reported. An [18F]AlF-NODA-labelled tetrazine was synthesised in excellent radiochemical yield...


2021 ◽  
Author(s):  
Samantha M. Sarrett ◽  
Outi Keinänen ◽  
Eric J. Dayts ◽  
Guillaume Dewaele-Le Roi ◽  
Cindy Rodriguez ◽  
...  

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Lin Qiu ◽  
Wujian Mao ◽  
Hongyan Yin ◽  
Hui Tan ◽  
Dengfeng Cheng ◽  
...  

The exceptional speed and biorthogonality of the inverse electron-demand Diels–Alder (IEDDA) click chemistry between 1,2,4,5-tetrazines and strained alkene dienophiles have made it promising in the realm of pretargeted imaging and therapy. During the past 10 years, the IEDDA-pretargeted strategies have been tested and have already proven capable of producing images with high tumor-to-background ratios and improving therapeutic effect. This review will focus on recent applications of click chemistry ligations in the pretargeted imaging studies of single photon emission computed tomography (SPECT), positron emission tomography (PET), and pretargeted radioimmunotherapy investigations. Additionally, the influence factors of stability, reactivity, and pharmacokinetic properties of TCO tag modified immunoconjugates and radiolabeled Tz derivatives were also summarized in this article, which should be carefully considered in the system design in order to develop a successful pretargeted methodology. We hope that this review will not only equip readers with a knowledge of pretargeted methodology based on IEDDA click chemistry but also inspire synthetic chemists and radiochemists to develop pretargeted radiopharmaceutical components in a more innovative way with various influence factors considered.


2019 ◽  
Author(s):  
Mathis Baalmann ◽  
Laura Neises ◽  
Sebastian Bitsch ◽  
Hendrik Schneider ◽  
Lukas Deweid ◽  
...  

A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA<sub>inv</sub>). With the aim of precisely generating branched protein chimeras, we systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. We demonstrate the efficiency and versatility of our conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technology enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines.


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