scholarly journals A click chemistry-based microRNA maturation assay optimized for high-throughput screening

2016 ◽  
Vol 52 (53) ◽  
pp. 8267-8270 ◽  
Author(s):  
Daniel A. Lorenz ◽  
Amanda L. Garner
Keyword(s):  
Click Chemistry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Diels Alder ◽  
Inverse Electron Demand ◽  
Electron Demand

A catalytic enzyme-linked click chemistry assay (cat-ELCCA) for Dicer-catalyzed pre-microRNA maturation was optimized to employ inverse-electron demand Diels–Alder (IEDDA) chemistry affording high-throughput screening capability.

scholarly journals Modular Strategy for the Construction of Radiometalated Antibodies for Positron Emission Tomography Based on Inverse Electron Demand Diels–Alder Click Chemistry

2011 ◽  
Vol 22 (10) ◽  
pp. 2048-2059 ◽  
Author(s):  
Brian M. Zeglis ◽  
Priya Mohindra ◽  
Gabriel I. Weissmann ◽  
Vadim Divilov ◽  
Scott A. Hilderbrand ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Click Chemistry ◽  
Diels Alder ◽  
Emission Tomography ◽  
Inverse Electron Demand ◽  
Positron Emission ◽  
Electron Demand

scholarly journals Specific Binding of Liposomal Nanoparticles through Inverse Electron-Demand Diels-Alder Click Chemistry

ChemistryOpen ◽  
2017 ◽  
Vol 6 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Christian Brand ◽  
Pasquale Iacono ◽  
Carlos Pérez-Medina ◽  
Willem J. M. Mulder ◽  
Moritz F. Kircher ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Specific Binding ◽  
Diels Alder ◽  
Inverse Electron Demand ◽  
Liposomal Nanoparticles ◽  
Electron Demand

scholarly journals A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates

2019 ◽  
Author(s):  
Mathis Baalmann ◽  
Laura Neises ◽  
Sebastian Bitsch ◽  
Hendrik Schneider ◽  
Lukas Deweid ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Protein Level ◽  
Product Formation ◽  
Diels Alder ◽  
Side Product ◽  
Efficient Technology ◽  
Inverse Electron Demand ◽  
Protein Conjugates ◽  
Scientific Disciplines ◽  
Electron Demand

A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA<sub>inv</sub>). With the aim of precisely generating branched protein chimeras, we systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. We demonstrate the efficiency and versatility of our conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technology enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines.

scholarly journals Establishment of the In Vivo Efficacy of Pretargeted Radioimmunotherapy Utilizing Inverse Electron Demand Diels-Alder Click Chemistry

2016 ◽  
Vol 16 (1) ◽  
pp. 124-133 ◽  
Author(s):  
Jacob L. Houghton ◽  
Rosemery Membreno ◽  
Dalya Abdel-Atti ◽  
Kristen M. Cunanan ◽  
Sean Carlin ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Diels Alder ◽  
In Vivo Efficacy ◽  
Inverse Electron Demand ◽  
Pretargeted Radioimmunotherapy ◽  
Electron Demand

scholarly journals A kit-based aluminium-[18F]fluoride approach to radiolabelled microbubbles

2021 ◽  
Author(s):  
Jin Teh ◽  
Marta Costa-Braga ◽  
Louis Allott ◽  
Chris Barnes ◽  
Javier Hernandez-Gil ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Radiochemical Yield ◽  
Diels Alder ◽  
Inverse Electron Demand ◽  
Electron Demand ◽  
18F Fluoride

The production of 18F-labelled microbubbles (MBs) via the aluminium-[18F]fluoride ([18F]AlF) radiolabelling method and facile inverse-electron-demand Diels-Alder (IEDDA) ‘click’ chemistry is reported. An [18F]AlF-NODA-labelled tetrazine was synthesised in excellent radiochemical yield...

scholarly journals Inverse electron demand Diels–Alder click chemistry for pretargeted PET imaging and radioimmunotherapy

2021 ◽  
Author(s):  
Samantha M. Sarrett ◽  
Outi Keinänen ◽  
Eric J. Dayts ◽  
Guillaume Dewaele-Le Roi ◽  
Cindy Rodriguez ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Pet Imaging ◽  
Diels Alder ◽  
Inverse Electron Demand ◽  
Electron Demand

scholarly journals Pretargeted Nuclear Imaging and Radioimmunotherapy Based on the Inverse Electron-Demand Diels–Alder Reaction and Key Factors in the Pretargeted Synthetic Design

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Lin Qiu ◽  
Wujian Mao ◽  
Hongyan Yin ◽  
Hui Tan ◽  
Dengfeng Cheng ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Single Photon ◽  
Photon Emission ◽  
Influence Factors ◽  
Nuclear Imaging ◽  
Alder Reaction ◽  
Diels Alder ◽  
Emission Computed Tomography ◽  
Inverse Electron Demand ◽  
Electron Demand

The exceptional speed and biorthogonality of the inverse electron-demand Diels–Alder (IEDDA) click chemistry between 1,2,4,5-tetrazines and strained alkene dienophiles have made it promising in the realm of pretargeted imaging and therapy. During the past 10 years, the IEDDA-pretargeted strategies have been tested and have already proven capable of producing images with high tumor-to-background ratios and improving therapeutic effect. This review will focus on recent applications of click chemistry ligations in the pretargeted imaging studies of single photon emission computed tomography (SPECT), positron emission tomography (PET), and pretargeted radioimmunotherapy investigations. Additionally, the influence factors of stability, reactivity, and pharmacokinetic properties of TCO tag modified immunoconjugates and radiolabeled Tz derivatives were also summarized in this article, which should be carefully considered in the system design in order to develop a successful pretargeted methodology. We hope that this review will not only equip readers with a knowledge of pretargeted methodology based on IEDDA click chemistry but also inspire synthetic chemists and radiochemists to develop pretargeted radiopharmaceutical components in a more innovative way with various influence factors considered.

scholarly journals A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates

2019 ◽  
Author(s):  
Mathis Baalmann ◽  
Laura Neises ◽  
Sebastian Bitsch ◽  
Hendrik Schneider ◽  
Lukas Deweid ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Protein Level ◽  
Product Formation ◽  
Diels Alder ◽  
Side Product ◽  
Efficient Technology ◽  
Inverse Electron Demand ◽  
Protein Conjugates ◽  
Scientific Disciplines ◽  
Electron Demand

A highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA<sub>inv</sub>). With the aim of precisely generating branched protein chimeras, we systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. We demonstrate the efficiency and versatility of our conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technology enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines.

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