scholarly journals Phenolic profile and antioxidant activity of different grape (Vitis vinifera L.) varieties

2019 ◽  
Vol 12 ◽  
pp. 04005 ◽  
Author(s):  
C. Di Lorenzo ◽  
F. Colombo ◽  
S. Biella ◽  
F. Orgiu ◽  
G. Frigerio ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mediterranean Area ◽  
Alcoholic Beverages ◽  
Vitis Vinifera L ◽  
Ionization Mass ◽  
Table Grapes ◽  
Grape Varieties

In the last years, the interest in non-alcoholic grape products has considerably increased. Table grapes are largely produced in the Mediterranean area and their consumption has raised worldwide. Beside the positive pattern of nutrients, table grapes could provide benefits on human health. Among the health-promoting substances contained in table grapes, flavonoids (mainly anthocyanins, flavan-3-ols and flavonols) seem to be the most interesting. The aim of this study was the characterization of the phenolic pattern of sixteen grape varieties, and the evaluation of the associated antioxidant activity. The methods were: 1) Folin-Cocalteau's assay for the quantification of total polyphenol content; 2) DPPH (1,1,-diphenil-2—picrylhydrazyl) assay and 3) ORAC (Oxygen Radical Absorbance Capacity) spectrophotometric assays for the assessment of radical scavenging activity; 4) High Performance Liquid Chromatography (HPLC) method, coupled with electrospray ionization mass spectrometer (ESI-MS) and DAD detector was developed in order to obtain the phenolic pattern of grape samples. Data obtained in this study underline that some table grape varieties can show interesting phenolic pattern independently from the presence or not of seeds. This observation suggests that selected varieties of seedless grapes could represent an interesting source of healthy compounds, satisfying consumers' preferences and reducing concerns versus alcoholic beverages.

scholarly journals Phenolic profile and biological activity of table grapes (Vitis vinifera L.)

2019 ◽  
Vol 15 ◽  
pp. 04005
Author(s):  
C. Di Lorenzo ◽  
F. Colombo ◽  
E. Sangiovanni ◽  
S. Biella ◽  
L. Regazzoni ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Array Detector ◽  
Vitis Vinifera L ◽  
Positive Effects ◽  
Table Grapes ◽  
Anti Inflammatory ◽  
Grape Varieties

Table grapes are largely produced and consumed in the Mediterranean area. Furthermore, in the last years, the commercial interest in table grapes and other non-alcoholic grape products has notably increased worldwide. In addition to the nutritional aspects, polyphenol compounds in fresh grapes could exert positive effects on human health. The aim of this study was the characterization of the phenolic pattern of sixteen grape varieties and the evaluation of the associated antioxidant capacity and anti-inflammatory activity at gastric level. The methods used were: 1) Folin-Cocalteau's assay for the quantification of total polyphenol content; 2) High-Performance Liquid Chromatography (HPLC) coupled with Diode-Array Detector (DAD) to perform the quantitative analysis of grapes phenolic fraction 3) DPPH (1,1,-diphenil-2-picrylhydrazyl) spectrophotometric assay for the assessment of radical scavenging activity; 4) assessment of IL-8 release from human gastric epithelial cells to evaluate the anti-inflammatory activity of grape extracts. Some grapes, including seedless varieties, showed that the phenolic pattern was highly correlated with the biological activities, and, in particular, with peel and seeds portion. These results suggest that selected grape varieties could represent, also for consumers who do not drink wine, a source of healthy compounds potentially able to counteract oxidative stress and gastric inflammation.

scholarly journals Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate

2017 ◽  
Vol 39 (1) ◽  
pp. 43 ◽  
Author(s):  
Mayara Neves Santos Guedes ◽  
Rafael Pio ◽  
Luana Aparecida Castilho Maro ◽  
Fabíola Fonseca Lage ◽  
Celeste Maria Patto de Abreu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Phenolic Contents

Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence of phenolic compounds. The results showed significant differences in the levels of phenolic compounds in the blackberry cultivars tested. Antioxidant activity was evaluated using the ABTS free radical and ranged from 2.7 ± 0.1 to 19 ± 2 μmole of Trolox equivalents per gram of sample (b.u.). These results are in good correlation with the phenolic contents of the blackberries tested. The Xavante blackberry cultivar had the highest levels of polyphenols that could be individually identified. Catechin polyphenols were found to be the main component in the blackberry varieties tested. 

scholarly journals Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Idaira Jerez-Martel ◽  
Sara García-Poza ◽  
Gara Rodríguez-Martel ◽  
Milagros Rico ◽  
Cristina Afonso-Olivares ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Arthrospira Platensis ◽  
Chlorogenic Acids ◽  
Nostoc Commune ◽  
Reverse Phase ◽  
Phenolic Profile ◽  
Methanolic Extracts

Aqueous and methanolic extracts of several microalgae (Ankistrodesmus sp., Spirogyra sp., Euglena cantabrica, and Caespitella pascheri) and cyanobacteria (Nostoc sp., Nostoc commune, Nodularia spumigena, Leptolyngbya protospira, Phormidiochaete sp., and Arthrospira platensis) were screened for their radical scavenging activity against the stable radical 1,1-diphenyl-2-picrylhydrazyl. Despite the fact that water was a more efficient solvent to extract greater amount of extractable substances, it seems that methanol was more efficient to extract a selected group of compounds with a higher antioxidant activity. In addition, the identification of 4 simple phenolics (gallic, syringic, protocatechuic, and chlorogenic acids) and the flavonoids (+) catechin and (-) epicatechin was carried out by using reverse phase high performance liquid chromatography. The strain Euglena cantabrica showed the highest concentration of phenolic compounds, particularly gallic and protocatechuic acids (5.87 and 2.97 mg per gram of dried biomass, resp.). Aqueous and methanolic extracts of microalgae Euglena cantabrica also exhibited the highest antioxidant activity, probably due to the presence of the high contents of phenolics.

scholarly journals Chromatographic and Spectroscopic Fingerprinting of Ficus carica and Evaluation of In Vitro Antioxidant Activity

2021 ◽  
Vol 25 (03) ◽  
pp. 677-682
Author(s):  
Amara Javaid
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Ficus Carica ◽  
In Vitro Antioxidant Activity

The present study was conducted to evaluate the in vitro antioxidant activity of Ficus carica, commonly known as fig. Methanol and ethanol extracts of F. carica leaves were subjected to 2, 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity assay where ascorbic acid being positive control had an IC50 value of 3.98±0.26 while methanol and ethanol fractions showed an IC50 of 101.76±1.12 and 93.12±1.17 respectively exhibiting their high antioxidant potential. DPPH assay was also performed on high performance liquid chromatography (HPLC) elutions. Most active antioxidant components in ethanol extract were eluted between 17–18 min, and those in methanol were eluted over 14–15 min and upon ultra-high performance liquid chromatography-mass spectrometery (Orbitrap Liquid Chromatography-Mass Spectrometry) were identified to be 13-Docosenamide, (Z)- for ethanol and ficusin for methanol fraction. Thus, it is concluded that these two components are most probable determinants of antioxidant potential of F. carica leaf extracts. © 2021 Friends Science Publishers

HPTLC analysis and in vitro antioxidant activity of aqueous bark extract of Erythrina variegata L.

2016 ◽  
Vol 63 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Kanakasabapathy Devaki
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Erythrina Variegata ◽  
High Antioxidant Activity ◽  
Layer Chromatography

Erythrina variegata L. is an important medicinal plant used in the preparations of Ayurvedic formulations used against several ailments. This study was carried out to investigate the presence of secondary metabolites using phytochemical screening, high-performance thin-layer chromatography (HPTLC) fingerprinting analysis and the antioxidant potential of the aqueous bark extract of E. variegata L. The secondary metabolites and the free radical scavenging activity were analyzed using standard protocols. The results obtained in the present study revealed that E. variegata has high antioxidant activity against free radicals based on phytoconstituents.

scholarly journals Antioxidant and Chelating Activity of NontoxicJatropha curcasL. Protein Hydrolysates Produced byIn VitroDigestion Using Pepsin and Pancreatin

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Santiago Gallegos Tintoré ◽  
Cristina Torres Fuentes ◽  
Javier Solorza Feria ◽  
Manuel Alaiz ◽  
Julio Girón Calle ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Cultures ◽  
Positive Impact ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Protein Hydrolysates ◽  
Ionization Mass ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Functional Components

The antioxidant and metal chelating activities inJ. curcasprotein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. Peptidic fractions with higher radical scavenging activity were analysed by matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant activity was determined by measuring inhibition of the oxidative degradation ofβ-carotene and by measuring the reactive oxygen species (ROS) in Caco-2 cell cultures. Cu2+and Fe2+chelating activities were also determined. The hydrolysates inhibited the degradation ofβ-carotene and the formation of ROS in Caco-2 cells. The lower molecular weight peptidic fractions from FPLC had stronger antioxidant activity in cell cultures compared with the hydrolysates, which correlated with a higher content in antioxidant and chelating amino acids. These fractions were characterized by a large presence of peptides with different molecular masses. The hydrolysates exhibited both Cu2+and Fe2+chelating activity. It was concluded thatJ. curcasis a good source of antioxidant and metal chelating peptides, which may have a positive impact on the economic value of this crop, as a potential source of food functional components.

scholarly journals Chemical characterization, fatty acid profile and antioxidant activity of Gustavia macarenensis fruit mesocarp and its oil from the Amazonian region of Ecuador as an unconventional source of vegetable oil

2019 ◽  
Vol 70 (2) ◽  
pp. 298
Author(s):  
J. J. Reyes Mera ◽  
R. Abreu-Naranjo ◽  
J. M. Alvarez-Suarez ◽  
D. Viafara
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Total Phenolic ◽  
Linear Discriminant ◽  
Chromatography Analysis

To our knowledge, this study is the first to report on the nutritional characterization, bioactive compounds and antioxidant activity of Amazonian G. macarenensis fruit. The fatty acid profile was determined using a high performance liquid chromatography analysis. The total phenolic content and antioxidant activity were determined using Folin Ciocalteu’s method and by radical scavenging activity, respectively. Moreover, a cluster analysis was carried out in order to classify the G. macarenensis fruit oil according to its fatty acid profile. Seven Fisher linear discriminant functions were obtained from the discriminant analysis. These models allow one to classify new fruits on the basis of their fatty acid profile. A high value for total lipids was obtained (53.57%). Its main components were palmitic and oleic acid. The TPC value (156.49 ± 2.62 mg GAE/Kg of oil) obtained from the G. macarenensis fruit pulp oil is higher than what was reported for some olive oil and Brazilian mango oil varieties.

scholarly journals β-ecdysone content and antioxidant capacity in different organs of Brazilian ginseng

2021 ◽  
Vol 51 (5) ◽  
Author(s):  
Lucas Dutra Zani da Silva Souza ◽  
Stéphane Raquel Almeida Velande da Fonseca ◽  
Ariana Ferrari ◽  
Daniele Fernanda Felipe
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Dpph Radical ◽  
Brazilian Ginseng ◽  
Dpph Radical Scavenging ◽  
Β Carotene

ABSTRACT: Plants that contain antioxidant compounds have attracted increasing interest for their vital role in the attenuation of oxidative damage caused by free radicals and in the treatment of various diseases. The present study investigated the β-ecdysone content and the antioxidant activity of Brazilian ginseng (Pfaffia glomerata) extracts obtained from inflorescences, stems, and roots. The P. glomerata extracts were tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, β-carotene bleaching test, and phosphomolybdenum method. The β-ecdysone content of P. glomerata extracts was measured by high-performance liquid chromatography (HPLC). The P. glomerata inflorescences showed the strongest DPPH radical scavenging activity and the strongest antioxidant activity in the β-carotene bleaching assay and phosphomolybdenum test. The roots showed the lowest antioxidant capacity in all of the assays. The concentration of β-ecdysone in the plant organs followed the following decreasing order: inflorescences > stems > roots. The present study showed that P. glomerata inflorescence extract had high antioxidant capacity that could be attributed to the presence of β-ecdysone.

scholarly journals Spectrum-Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Yangyin Tongnao Prescription

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Li Yu ◽  
Yangyang Zhang ◽  
Xixi Zhao ◽  
Yu He ◽  
Haofang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Linear Regression Analysis ◽  
Radical Scavenging ◽  
Multiple Linear Regression Analysis ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Effect Relationship ◽  
Spectrum Effect

Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.

scholarly journals DETERMINATION OF PHYSICOCHEMICAL PROPERTIES, ANTIOXIDANT CONSTITUENTS BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF CUCURBITA MAXIMA SEEDS

2018 ◽  
Vol 11 (3) ◽  
pp. 280
Author(s):  
Pinki Pal ◽  
Shiv Bhadra Singh ◽  
Aaditya Singh
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Layer Chromatography

 Objective: The present study is designed to evaluate preliminary phytochemical constituents, physiochemical properties evaluation, free radical scavenging activity, and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis of Cucurbita maxima seed oil.Methods: Total phenol content, total flavonoid content, total tannin content, 1, 1-diphenyl, 2-picryl-hydrazyl (DPPH) radical scavenging method, and HPTLC fingerprinting analysis for compounds responsible for antioxidant activity were used.Result: Phytochemical screening shows the presence of constituents such as alkaloids, glycosides, carbohydrates, fixed oil and fats, phytosterols, saponin, phenolic compounds, tannins, proteins, amino acids, gums, and mucilage. Physicochemical studies show that the oil contains an acid value, saponification value, iodine value of 9.53 mg KOH/g, 183 mg KOH/g, and 116.51 mg I2/100 g, respectively. Total phenol content was 3.9498±3.2 mg gallic acid equivalent/g, total flavonoid content was 56.115±4.6 mg rutin equivalent/g, and total tannin content was 19.4±0.40 mg tannic acid equivalent/g. The radical scavenging activity by DPPH Model shows that the methanolic extract has maximum antioxidant activity with IC50 value of 238.31. HPTLC fingerprinting also proofs the presence of phenol and flavonoid compounds in the seed oil.Conclusion: The results in the paper show that the C. maxima seed oil is an natural antioxidant and can be used for the treatment of various disorders.

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