scholarly journals RESPONSE OF POTASSIUM RETENTIVITY AND SURVIVAL OF YEAST TO FAR-ULTRAVIOLET, NEAR-ULTRAVIOLET AND VISIBLE, AND X-RADIATION

1958 ◽  
Vol 41 (4) ◽  
pp. 693-702 ◽  
Author(s):  
A. K. Bruce
Keyword(s):  
Yeast Cell ◽  
Dose Response ◽  
X Rays ◽  
Anaerobic Conditions ◽  
Aerobic Conditions ◽  
Differential Effects ◽  
Near Ultraviolet ◽  
X Irradiation ◽  
Far Ultraviolet ◽  
Control Survival

Potassium retentivity and survival of yeast were studied after exposure to various kinds and conditions of irradiation. The radiations used were: 2537 A ultraviolet, 3500 to 4900 A long-ultraviolet and short visible, and 250 kvp1 x-rays. Both potassium retentivity and survival are decreased by these radiations. The dose-response of survival is about 16 times as sensitive as is potassium retentivity after 2537 A irradiation. Potassium retentivity is about twice as sensitive as survival after irradiation of 3500 to 4900 A. Survival after x-irradiation under aerobic conditions is five times as sensitive as potassium retentivity. Survival of cells irradiated with x-rays under anaerobic conditions was about half as sensitive as under aerobic conditions. The response of potassium retentivity to x-radiation at 25°C. under anaerobic conditions is only slightly affected below 160 kr, at which dose the slope abruptly increases to that obtained under aerobic conditions; lowering the temperature to 0°C. moves this point to about 300 kr. These differential effects are indicative of interaction of radiations with the yeast cell at sites that independently control survival and the retention of potassium.

FINE STRUCTURE MAPPING IN YEAST WITH SUNLAMP RADIATION

Genetics ◽  
1974 ◽  
Vol 76 (4) ◽  
pp. 723-733
Author(s):  
Christopher W Lawrence ◽  
Roshan Christensen
Keyword(s):  
Fine Structure ◽  
Dose Response ◽  
Gamma Ray ◽  
X Rays ◽  
Structure Mapping ◽  
Mapping Procedure ◽  
X Ray ◽  
Linear Dose ◽  
Near Ultraviolet ◽  
Ionizing Radiations

ABSTRACT The X-ray mapping procedure of Manney and Mortimer (1964) is the most widely applicable and convenient method for fine structure analysis in yeast, but suffers the disadvantage that suitable X-ray machines or gamma ray sources are very expensive. Although many other recombinogens are known, none gives a linear dose-response like X-rays and few are as convenient or give as reproducible results. Experiments with Saccharomyces cerevisiae reported in this paper show, however, that the near-ultraviolet radiation emitted by fluorescent sunlamps gives linear dose-response relations, as reproducible results as ionizing radiations, and map distances which correlate highly with those obtained by using 60Co gamma rays. It is suggested that this convenient recombinogen may be a suitable low-cost substitute for ionizing radiations in fine structure mapping.

Response of newt larvae to X-irradiation

1970 ◽  
Vol 48 (5) ◽  
pp. 1017-1021
Author(s):  
G. B. Friedmann ◽  
H. M. McCurdy ◽  
F. T. Algard
Keyword(s):  
Life Span ◽  
Dose Response ◽  
Progenitor Cell ◽  
X Rays ◽  
Cell Type ◽  
Taricha Torosa ◽  
Age Dependent ◽  
X Irradiation ◽  
Complete Metamorphosis

Graded doses of from 200 to 2000 rad of x-rays were administered to premetamorphic Taricha torosa larvae of various ages. General stunting, specific malformation, pigmentary damage, and a very shortened life-span occurred at all doses. There is an age-dependent dose response; some animals exposed to 200 and 600 rad survived to complete metamorphosis. Evidence is presented supporting a view that postmetamorphic epidermal melanocytes may be derived largely by division of an unpigmented progenitor cell type.

scholarly journals Differential Effects of Alpha-Particle Radiation and X-Irradiation on Genes Associated with Apoptosis

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Vinita Chauhan ◽  
Matthew Howland ◽  
Jeremy Chen ◽  
Barbara Kutzner ◽  
Ruth C. Wilkins
Keyword(s):  
Gene Expression ◽  
Flow Cytometry ◽  
Cell Death ◽  
X Rays ◽  
Particle Radiation ◽  
Monocytic Cells ◽  
Differential Effects ◽  
X Irradiation ◽  
Apoptotic Genes ◽  
Higher Doses

This study examined differential effects of alpha-(α-) particle radiation and X-rays on apoptosis and associated changes in gene expression. Human monocytic cells were exposed toα-particle radiation and X-rays from 0 to 1.5 Gy. Four days postexposure, cell death was measured by flow cytometry and 84 genes related to apoptosis were analyzed using real-time PCR. On average, 33% of the cells were apoptotic at 1.5 Gy ofα-particle radiation. Transcript profiling showed statistical expression of 15 genes at all three doses tested. Cells exposed to X-rays were <5% apoptotic at~1.5 Gy and induced less than a 2-fold expression in 6 apoptotic genes at the higher doses of radiation. Among these 6 genes, Fas and TNF-αwere common to theα-irradiated cells. This data suggests thatα-particle radiation initiates cell death by TNF-αand Fas activation and through intermediate signalling mediators that are distinct from X-irradiated cells.

scholarly journals ROLE OF DNA SEQUENCES IN GENETIC RECOMBINATION IN THE ISO-1-CYTOCHROME c GENE OF YEAST. I. DISCREPANCIES BETWEEN PHYSICAL DISTANCES AND GENETIC DISTANCES DETERMINED BY FIVE MAPPING PROCEDURES

Genetics ◽  
1975 ◽  
Vol 79 (3) ◽  
pp. 397-418
Author(s):  
Carol W Moore ◽  
Fred Sherman
Keyword(s):  
Ultraviolet Light ◽  
Dna Sequences ◽  
Meiotic Recombination ◽  
Genetic Recombination ◽  
Genetic Distances ◽  
Nucleotide Sequences ◽  
X Rays ◽  
Recombination Rates ◽  
Near Ultraviolet ◽  
X Irradiation

ABSTRACT Recombination rates have been examined in two-point crosses of various defined cyc1 mutants using five mapping methods. Nucleotide sequences of mutant codons were identified in previous studies from alterations in functional iso-1-cytochromes c produced by intragenic revertants. Heteroallelic diploids were analyzed for rates of mitotic recombination that occurred spontaneously and that were induced with X-rays, ultraviolet light and the near-ultraviolet light emitted by sunlamps, as well as rates of meiotic recombination that occur after sporulation. Frequencies of both mitotic and meiotic recombination do not necessarily correspond with physical distances separating altered nucleotides. The most extreme discrepancy involved two adjacent intervals of thirteen base pairs which differed approximately thirtyfold in their spontaneous and X-ray-induced recombination rates. Marked disproportions between genetic and physical distances appear to be due to the interaction of the two nucleotide sequences in the heteroallelic combination and not to the sequences of the mutant codons alone. Recombination values that were obtained by all five methods could not be used to establish the correct order of mutant sites. Relationships of the recombination rates for the various pairwise crosses are different after mitosis from those after meiosis, suggesting that these two recombinational processes are to some extent different in their dependence on particular nucleotide configurations. On the other hand, the relationships of the rates induced by UV-, sunlamp- and X-irradiation were identical or very similar. In addition to the intrinsic properties of the alleles affecting frequencies of mitotic and meiotic recombination rates, two- to threefold variations in recombination rates could be attributed to genetic backgrounds.

Eds Of Radioactive Particles By Self-Induced Fluorescence

1990 ◽  
Vol 48 (2) ◽  
pp. 238-239
Author(s):  
Gregory L. Finch ◽  
Richard G. Cuddihy
Keyword(s):  
Electron Beam Irradiation ◽  
Nuclear Reactor ◽  
X Rays ◽  
Reactor Accident ◽  
Internal Radiation ◽  
X Ray ◽  
External Sources ◽  
X Irradiation ◽  
Available Information ◽  
Individual Particles

The elemental composition of individual particles is commonly measured by using energydispersive spectroscopic microanalysis (EDS) of samples excited with electron beam irradiation. Similarly, several investigators have characterized particles by using external monochromatic X-irradiation rather than electrons. However, there is little available information describing measurements of particulate characteristic X rays produced not from external sources of radiation, but rather from internal radiation contained within the particle itself. Here, we describe the low-energy (< 20 KeV) characteristic X-ray spectra produced by internal radiation self-excitation of two general types of particulate samples; individual radioactive particles produced during the Chernobyl nuclear reactor accident and radioactive fused aluminosilicate particles (FAP). In addition, we compare these spectra with those generated by conventional EDS.Approximately thirty radioactive particle samples from the Chernobyl accident were on a sample of wood that was near the reactor when the accident occurred. Individual particles still on the wood were microdissected from the bulk matrix after bulk autoradiography.

The Reaction of Thiol Compounds with the Vitamin-K Dependent Clotting Factors

1969 ◽  
Vol 21 (03) ◽  
pp. 573-579 ◽  
Author(s):  
P Fantl
Keyword(s):  
Prothrombin Time ◽  
Vitamin K ◽  
Anaerobic Conditions ◽  
Clotting Factors ◽  
Thiol Compounds ◽  
Aerobic Conditions ◽  
One Stage ◽  
Factor Ii ◽  
Ph Dependent ◽  
The One

SummaryTreatment of human and dog oxalated plasma with 0.2 to 1.0 × 10−1 M 2.3-dithiopropanol (BAL) or dithiothreitol (DTT) at 2–4° C for 30 min results in the reduction of the vitamin-K dependent clotting factors II, VII, IX and X to the respective-SH derivatives. The reaction is pH dependent. Under aerobic conditions the delayed one stage prothrombin time can be partly reversed. Under anaerobic conditions a gradual prolongation of the one stage prothrombin time occurs without reversal.In very diluted plasma treated with the dithiols, prothrombin can be converted into thrombin if serum as source of active factors VII and X is added. In contrast SH factors VII, IX and X are inactive in the specific tests. Reoxidation to active factors II, VII, IX and X takes place during adsorption and elution of the SH derivatives. The experiments have indicated that not only factor II but also factors VII, IX and X have active-S-S-centres.

Sources of Variability in Dose Response Platelet Aggregometry

1985 ◽  
Vol 53 (02) ◽  
pp. 219-220 ◽  
Author(s):  
M V Vickers ◽  
S G Thompson
Keyword(s):  
Dose Response ◽  
Maximum Velocity ◽  
Adenosine Diphosphate ◽  
Method Error ◽  
Platelet Aggregability ◽  
Differential Effects ◽  
Platelet Aggregometry ◽  
Simultaneous Comparison ◽  
Aggregation Parameters ◽  
Very High

SummaryAn experiment designed to assess the components of variability of a number of measures of platelet aggregability showed that the ADP ED50 (the estimated dose of adenosine diphosphate at which primary aggregation occurs at half its maximum velocity) had the least method error of any of the parameters measured, but that none had a very high between-person component of variability. A simultaneous comparison of a syringe technique and a free-flowing technique for venepuncture revealed no differential effects on the aggregation parameters measured. An enforced increase in the stirring speed in the aggregometer led to an experiment which showed that such a change did not apparently affect the ED50s.

scholarly journals THE INTERCELLULAR DISTRIBUTION OF MUTATIONS INDUCED IN OOCYTES OF DROSOPHILA MELANOGASTER BY CHEMICAL AND PHYSICAL MUTAGENS

Genetics ◽  
1979 ◽  
Vol 92 (1) ◽  
pp. 151-160
Author(s):  
H Traut
Keyword(s):  
Drosophila Melanogaster ◽  
Mitomycin C ◽  
Mutation Frequency ◽  
Lethal Mutation ◽  
X Rays ◽  
Mutagenic Treatment ◽  
X Chromosomes ◽  
Recessive Lethal ◽  
Lethal Mutations ◽  
X Irradiation

ABSTRACT When females of Drosophila melanogaster are treated with chemical or physical mutagens, not only in one but also in both of the two homologous X chromosomes of a given oocyte, a recessive sex-linked lethal mutation may be induced. A method is described that discriminates between such "single" and "double mutations." A theory is developed to show how a comparison between the expected and the observed frequency of double mutations yields an indication of the intercellular distribution (random or nonrandom) of recessive lethal mutations induced by mutagenic agents in oocytes and, consequently, of the distribution (homogeneous or nonhomogeneous) of those agents.—Three agents were tested: FUdR (12.5, 50.0 and 81.0,μg/ml), mitomycin C (130.0 μg/ml) and X rays (2000 R, 150 kV). After FUdR feeding, no increase in the mutation frequency usually observed in D. melanogaster without mutagenic treatment was obtained (u=0.13%, namely three single mutations among 2332 chromosomes tested). After mitomycin C feeding, 104. single and three double mutations were obtained. All of the 50 mutations observed after X irradiation were single mutations. The results obtained in the mitomycin C and radiation experiments favor the assumption of a random intercellular distribution of recessive lethal mutations induced by these two agents in oocytes of D. melanogaster. Reasons are discussed why for other types of mutagenic agents nonrandom distributions may be observed with our technique.

Performance of aerobic granular sludge at variable circulation rate in anaerobic–aerobic conditions

2014 ◽  
Vol 69 (11) ◽  
pp. 2252-2257 ◽  
Author(s):  
Hasnida Harun ◽  
Aznah Nor Anuar ◽  
Zaini Ujang ◽  
Noor Hasyimah Rosman ◽  
Inawati Othman
Keyword(s):  
Municipal Wastewater ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
Soy Sauce ◽  
Volume Index ◽  
Aerobic Granular Sludge ◽  
Circulation Rate ◽  
Anaerobic Conditions ◽  
Aerobic Conditions ◽  
Mixed Liquor

Aerobic granular sludge (AGS) has been applied to treat a broad range of industrial and municipal wastewater. AGS can be developed in a sequencing batch reactor (SBR) with alternating anaerobic–aerobic conditions. To provide anaerobic conditions, the mixed liquor is allowed to circulate in the reactor without air supply. The circulation flow rate of mixed liquor in anaerobic condition is the most important parameter of operation in the anaerobic-AGS processes. Therefore, this study investigates the effect of circulation rate on the performance of the SBR with AGS. Two identical reactors namely R1 and R2 were operated using fermented soy sauce wastewater at circulation rate of 14.4 and 36.0 l/h, respectively. During the anaerobic conditions, the wastewater was pumped out from the upper part of the reactor and circulated back into the bottom of the reactor for 230 min. A compact and dense AGS was observed in both reactors with a similar diameter of 2.0 mm in average, although different circulation rates were adopted. The best reactor performance was achieved in R2 with chemical oxygen demand removal rate of 89%, 90% total phosphorus removal, 79% ammonia removal, 10.1 g/l of mixed liquor suspended solids and a sludge volume index of 25 ml/g.

Differential Effects of X-Rays and High-Energy 56Fe Ions on Human Mesenchymal Stem Cells

2009 ◽  
Vol 73 (3) ◽  
pp. 869-877 ◽  
Author(s):  
Kyle Kurpinski ◽  
Deok-Jin Jang ◽  
Sanchita Bhattacharya ◽  
Bjorn Rydberg ◽  
Julia Chu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
High Energy ◽  
X Rays ◽  
Differential Effects
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