[Invited] Electrochemical Impedance Sensor for Non-invasive Living Cell Monitoring toward CMOS Cell Culture Monitoring Platform

2021 ◽  
Author(s):  
Shigeyasu Uno
Keyword(s):  
Cell Culture ◽  
Living Cell ◽  
Electrochemical Impedance ◽  
Non Invasive ◽  
Cell Monitoring ◽  
Impedance Sensor ◽  
Monitoring Platform ◽  
Culture Monitoring

Non-invasive monitoring of living cell culture by lensless digital holography imaging

2011 ◽  
Vol 9 (3) ◽  
pp. 030901-30904 ◽  
Author(s):  
王云新 Yunxin Wang ◽  
王大勇 Dayong Wang ◽  
赵洁 Jie Zhao ◽  
杨怡殊 Yishu Yang ◽  
肖向茜 Xiangqian Xiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Living Cell ◽  
Digital Holography ◽  
Invasive Monitoring ◽  
Non Invasive

Non-invasive monitoring for living cell culture with lensless Fourier transform digital holography microscopy

2010 ◽  
Author(s):  
Yunxin Wang ◽  
Dayong Wang ◽  
Jie Zhao ◽  
Yan Li ◽  
Puhui Meng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fourier Transform ◽  
Living Cell ◽  
Digital Holography ◽  
Invasive Monitoring ◽  
Non Invasive

scholarly journals Tethered Lipid Membranes as a Nanoscale Arrangement Towards Non-Invasive Analysis of Acute Pancreatitis

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 755
Author(s):  
Rima Budvytyte ◽  
Akvile Milasiute ◽  
Dalius Vitkus ◽  
Kestutis Strupas ◽  
Aiste Gulla ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Lipid Membranes ◽  
Electrochemical Impedance ◽  
Membrane Integrity ◽  
Electrochemical Technique ◽  
Label Free ◽  
Non Invasive ◽  
Novel Approach ◽  
Analytical System ◽  
Shock Proteins

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

An electrochemical impedance sensor for the label-free ultrasensitive detection of interleukin-6 antigen

2013 ◽  
Vol 178 ◽  
pp. 310-315 ◽  
Author(s):  
Ting Yang ◽  
Shun Wang ◽  
Huile Jin ◽  
Weiwei Bao ◽  
Shaoming Huang ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Electrochemical Impedance ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Impedance Sensor

scholarly journals Pinhole microLED Array as Point Source Illumination for Miniaturized Lensless Cell Monitoring Systems

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 866 ◽  
Author(s):  
Shinta Mariana ◽  
Gregor Scholz ◽  
Feng Yu ◽  
Agus Budi Dharmawan ◽  
Iqbal Syamsu ◽  
...  
Keyword(s):  
Image Quality ◽  
Light Emitting Diode ◽  
Free Cell ◽  
Monitoring Systems ◽  
Label Free ◽  
Light Emitting ◽  
Non Invasive ◽  
Cell Monitoring ◽  
Blue Spectral Region ◽  
Led Arrays

Pinhole‐shaped light‐emitting diode (LED) arrays with dimension ranging from 100 μm down to 5 μm have been developed as point illumination sources. The proposed microLED arrays, which are based on gallium nitride (GaN) technology and emitting in the blue spectral region (λ = 465 nm), are integrated into a compact lensless holographic microscope for a non‐invasive, label‐free cell sensing and imaging. From the experimental results using single pinhole LEDs having a diameter of 90 μm, the reconstructed images display better resolution and enhanced image quality compared to those captured using a commercial surface‐mount device (SMD)‐based LED.

Impedance-based drug-resistance characterization of colon cancer cells through real-time cell culture monitoring

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121441
Author(s):  
Fuentes-Vélez Susana ◽  
Fagoonee Sharmila ◽  
Sanginario Alessandro ◽  
Gallo Valentina ◽  
Riganti Chiara ◽  
...  
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Real Time ◽  
Colon Cancer Cells ◽  
Culture Monitoring

Biodegradable Mg for Bone Implants: Corrosion and Osteoblast Culture Studies

2008 ◽  
Author(s):  
YeoHeung Yun ◽  
Zhongyun Dong ◽  
Dianer Yang ◽  
Vesselin Shanov ◽  
Zhigang Xu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Electrochemical Impedance ◽  
Culture Media ◽  
Corrosion Current ◽  
Visual Observation ◽  
Open Circuit ◽  
Biodegradable Implant ◽  
Cell Culture Media ◽  
X Ray ◽  
Implant Material

Corrosion and cell culture experiments were performed to evaluate magnesium (Mg) as a possible biodegradable implant material. The corrosion current and potential of a Mg disk were measured in different physiological solutions. The corrosion currents in cell culture media were found to be higher than in deionized water, which verifies that corrosion of Mg occurs faster in chloride solution. Weight loss, open-circuit potential, and electrochemical impedance spectroscopy measurements were also performed. The Mg specimens were also characterized using an environmental scanning electron microscope and energy-dispersive x-ray analysis (EDAX). The x-ray analysis showed that in the cell culture media a passive interfacial layer containing oxygen, chloride, phosphate, and potassium formed on the samples. U2OS cells were then co-cultured with a Mg specimen for up to one week. Based on visual observation, cell growth and function were not significantly altered by the presence of the corroding Mg sample. These initial results indicate that Mg may be suitable as a biodegradable implant material. Future work will develop small sensors to investigate interfacial biocompatibility of Mg implants.

scholarly journals A New Non-invasive Technique for Measuring 3D-Oxygen Gradients in Wells During Mammalian Cell Culture

2020 ◽  
Vol 8 ◽  
Author(s):  
Carlos J. Peniche Silva ◽  
Gregor Liebsch ◽  
Robert J. Meier ◽  
Martin S. Gutbrod ◽  
Elizabeth R. Balmayor ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Invasive Technique ◽  
Non Invasive ◽  
Oxygen Gradients
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