Detection of Lung Cancer Cells in Solutions Using a Terahertz Chemical Microscope

Cancer genome analysis has recently attracted attention for personalized cancer treatment. In this treatment, evaluation of the ratio of cancer cells in a specimen tissue is essential for the precise analysis of the genome. Conventionally, the evaluation takes at least two days and depends on the skill of the pathologist. In our group, a terahertz chemical microscope (TCM) was developed to easily and quickly measure the number of cancer cells in a solution. In this study, an antibody was immobilized on a sensing plate using an avidin-biotin reaction to immobilize it for high density and to improve antibody alignment. In addition, as the detected terahertz signals vary depending on the sensitivity of the sensing plate, the sensitivity was evaluated using pH measurement. The result of the cancer cell detection was corrected using the result of pH measurement. These results indicate that a TCM is expected to be an excellent candidate for liquid biopsies in cancer diagnosis.

Development of an optical pH measurement system based on colorimetric effect

<p>This paper proposed an optical pH measurement system developed to measure the changes of pH levels based on colorimetric reactions with phenol red reagent. The optical sensing was achieved through the implementation of a pair of light emitting diode (LED) and photodiode. The detection mechanism was based on different absorbance of light intensity at pH values of 2.36, 8.32, 9.08 and 12.83. The data processing method was carried out using LabVIEW software and interfaced with NI USB DAQ 6008. The measured voltage showed a good correlation in relation to the pH level with R2 equals to 0.9624. This relationship was used as a calibration curve in the final system testing. The final measurement of pH showed a good agreement between the actual and measured values with an error of less than 5%, thus indicating the reliability of the proposed system.</p>

How Ph.D. Students Start a Research

In education pedagogy, its necessary to prioritize tasks and jobs. Learn operating the reactor or required apparatus but most important first. Regarding measuring techniques (PSD measurement, pH measurement, XRD, SEM/EDS), learn more basic and fundamental techniques first followed by complimentary techniques. Regarding mineral carbonation, operation of reactor is a basic need followed by particle size measurement using Malvern Mastersizer and powders samples XRD analysis and identifying the phases and verifying those phases through TGA analysis. This article illustrates author personal research of 4 initial 4 months duration. Month wise progress is presented in this article in an interesting way.

Unified pH measurement v1

The purpose of this document is to present technical guidance of measuring pHabsH2O difference (ΔpHabsH2O value) between two solutions by differential potentiometry in a cell with two compartments connected by a salt bridge filled with an ionic liquid.