ALPHA-2-MACROGLOBULIN AND THE QUESTION OF A PROTEIN CARRIER FOR INSULIN IN HUMAN SERUM

1965 ◽  
Vol 43 (6) ◽  
pp. 885-891 ◽  
Author(s):  
Herbert Geerling ◽  
Otakar V. Sirek
Keyword(s):  
Blood Serum ◽  
Binding Capacity ◽  
Specific Protein ◽  
Test Tube ◽  
Radioactive Material ◽  
Constant Amount ◽  
Agar Gel ◽  
Protein Carrier ◽  
Normal Individuals ◽  
Specific Insulin

Blood serum from presumably normal individuals was investigated for its insulin-I131 binding capacity by Immunoelectrophoresis and subsequent radioautography, by immunoprecipitation in the test tube, and by preparative ultracentrifugation.Binding of radioactive insulin to alpha-2-macrogiobulin was demonstrated by immunoelectrophoresis, but the fact that radioactivity was present also in a number of other protein precipitates weakened the argument for a specific insulin carrier in blood. Nonspecific trapping of radioactive material may have occurred in the course of formation of protein precipitates in agar gel because immunoprecipitates produced in the test tube with the same polyvalent antiserum did not contain appreciable amounts of radioactivity.Results of experiments in the preparative ultracentrifuge indicated that neither multiple dilutions nor addition of pure human alpha-2-macroglobulin to serum produced measurable differences in the distribution of radioactivity following incubation and spinning with a constant amount of insulin-I131.The results of these studies do not lend support to the concept of alpha-2-macroglobulin being a specific protein carrier of insulin in blood.

THYROXINE BINDING PROTEINS OF HUMAN SERUM AT PHYSIOLOGICAL pH

1969 ◽  
Vol 62 (2) ◽  
pp. 217-233 ◽  
Author(s):  
Amirav Gordon ◽  
Theodore Coutsoftides
Keyword(s):  
Binding Capacity ◽  
Specific Protein ◽  
Agar Gel ◽  
Binding Characteristics ◽  
Protein Carriers ◽  
Relative Binding ◽  
Maximal Binding ◽  
Tris Maleate ◽  
Agar Gel Electrophoresis ◽  
Capacity Data

ABSTRACT The binding characteristics of human TBG, TBPA and TBA of T4 and T3 were studied by agar gel electrophoresis in a variety of buffers. The highest maximal binding capacities of TBG and TBPA were found in Hank's balanced salt solution at pH 7.4. Phosphate and tris maleate ions at this pH resulted in the reduction of the thyroxine maximal binding capacity of TBG and TBPA. It was also shown that pH, ionic constitution and molarity of the system affect the distribution of tracer T4125I among the specific protein carriers. Thyroxine binding inhibitors were shown to affect TBPA, TBA and TBG at a physiological pH like the effect described at other pH levels. The relative binding potency of TBPA for thyroxine:DNP:salicylate:penicillin was in a ratio of 670:250:10:1, respectively. An attempt to deal with the binding capacity data by the Scatchard technique is described.

A Different Way for Detecting Anaemia Disease using Bioelectronics Circuit

2021 ◽  
Vol 16 ◽  
pp. 1-5
Author(s):  
Manthan S Manavadaria
Keyword(s):  
Blood Serum ◽  
Everyday Life ◽  
Human Blood ◽  
Human Platelet ◽  
Clinical Science ◽  
Human Blood Serum ◽  
Testing Technique ◽  
Normal Individuals ◽  
Similar Information ◽  
Entire World

All in all, clinical science characterizes anaemia disease as less tally of a red platelet. This illness is exceptionally successive in India and a few pieces of the entire world. This isn't straightforwardly influencing illness however the maker and initiator of numerous other blood-related issues. Biomedical has effectively built up a testing technique for the recognizable proof of such infection and sorted out the strategy for computing the quantity of check or scope of red platelets in human bodies. In view of a correlation of such information with ordinary human blood attributes specialists can distinguish the level of the pallor and its connected stage. This requires time like conventional blood revealing just as the precision of testing philosophy. With the help of this article, another method of recognizing similar information inside a brief timeframe is introduced. By processing the ordinary human platelet check with anaemia influenced blood through the bioelectronics circuit, it will be useful to sort out the presence of the illness. Rather than utilizing genuine blood, a substance blend comparable to human blood serum has been thought of and for sickness, synthetic creation has been modified. This may change results for genuine human blood however then circuit changes may assist us with improving the outcomes. The sugar meter and heart meter are as of now utilized in everyday life by normal individuals without the assistance of specialists. These commonsense outcomes may lead such instrument makers for building up the gadget for sickliness identification.

New reaction for recognition of pregnancy and gender of the intrauterine fetus

1926 ◽  
Vol 22 (1) ◽  
pp. 106
Author(s):  
V. Gruzdev
Keyword(s):  
Sodium Chloride ◽  
Blood Serum ◽  
Test Tube ◽  
And Gender ◽  
Made In

Sellheim (Zentr. F. Gyn., 1925, no. 32) the trace describes this reaction, which he calls the Lttge-Mertz-Berger reaction: 1 cubic meter is poured into a test tube. sant. the blood serum of a woman in whom pregnancy is suspected, or the sex of the fetus of which they want to find out, then add here a certain amount (the amount of this S. does not indicate) extracts from the placenta or testicles, mixed with a solution of sodium chloride (in what proportion - S. also does not indicates, but only says that this extract can be obtained ready-made, in ampoules, from the Wolff company in Bielefeld).

scholarly journals Serum epidemiological survey and risk factors investigation for lentivirus in goats from Sisal Region, Bahia, Brazil

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Danielle Nobre Santos Pinheiro ◽  
Joselito Nunes Costa ◽  
Thiago Sampaio de Souza ◽  
Vanderlan Warlington Souza Santos ◽  
Dalva Alana Azevedo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Blood Serum ◽  
Epidemiological Survey ◽  
Control Measures ◽  
Breeding Systems ◽  
Agar Gel ◽  
Pattern Type ◽  
Significant Difference ◽  
Males And Females

ABSTRACT: This study aimed to carry out a serum epidemiological survey of goat arthritis encephalitis in the sisal region of Bahia, Brazil, and to evaluate risk factors. We evaluated 831 samples of goat blood serum among males and females older than six months, from 49 farms distributed among the municipalities of Araci, Cansanção, Conceição do Coité, Itiúba, Monte Santo, Nordestina, Queimadas, Santa Luz, São Domingos and Valente. An epidemiological questionnaire for the analysis of possible risk factors was applied. All sera were tested for immune-agar gel. The seroprevalence obtained in surveyed herds was 1.56% (13/831). There was significant difference (p<0.05) for animal racial pattern, type of farming and breeding systems. However, when considering herds with predominantly dairy breeds (Saanen and Alpine Pardo), the seropositivity in animals from Valente, Conceição do Coité and São Domingos amounted to 5.06% (12/237). In these municipalities, from 14 properties analyzed, five (38.5%) had at least one positive-testing animal. This result is extremely worrying when one considers that few control measures are adopted by farmers to prevent the goat arthritis encephalitis in the most important dairy region of Bahia state.

scholarly journals ON THE MODE OF ACTION OF SULFANILAMIDE IN EXPERIMENTAL STREPTOCOCCUS EMPYEMA

1937 ◽  
Vol 66 (5) ◽  
pp. 535-548 ◽  
Author(s):  
Frederick P. Gay ◽  
Ada R. Clark
Keyword(s):  
Blood Serum ◽  
Mode Of Action ◽  
Direct Evidence ◽  
Test Tube ◽  
Visceral Pleura ◽  
Cell Reaction ◽  
Animal Body ◽  
Natural Defense ◽  
Repeated Doses ◽  
Pleural Exudate

Sulfanilamide prevents the evolution of an invariably fatal streptococcus empyema in rabbits when it is given repeatedly and in sufficient doses subcutaneously. Complete sterilization of the inoculated cavity occurs on approximately the 2nd day. The serum, defibrinated blood and artificial pleural exudate of similarly treated animals inhibits the growth of the same streptococcus in the test tube but even repeated doses of such treated blood serum fail to sterilize the culture. The coccal chains grown in such drugged serum are elongated and present pleomorphic and metachromatic organisms and may give rise to colonies that are at first less predominantly mucoid in appearance. Such organisms have, however, lost little if any of their virulence. Cooperation on the part of locally derived clasmatocytes is apparently required in complete sterilization of the animal body. This conclusion is reached not only by a process of exclusion from comparison with the test tube results, but through the direct histological demonstration of a precocious and increasing mobilization of clasmatocytes in the parietal and visceral pleura of treated animals. In other words, sulfanilamide apparently produces a bacteriostasis sufficiently marked to protect the accumulated leucocytes and to allow the natural defense macrophages to accumulate. There is direct evidence that the drug does not in itself stimulate the mobilization of the macrophages. There is no evidence that the cell reaction which finally accounts for disposal of the organisms is other than local.

scholarly journals The "Normandy" variant of von Willebrand disease: characterization of a point mutation in the von Willebrand factor gene

Blood ◽  
1991 ◽  
Vol 77 (9) ◽  
pp. 1937-1941 ◽  
Author(s):  
C Gaucher ◽  
S Jorieux ◽  
B Mercier ◽  
D Oufkir ◽  
C Mazurier
Keyword(s):  
Amino Acid ◽  
Point Mutation ◽  
Von Willebrand Factor ◽  
Binding Capacity ◽  
Von Willebrand Disease ◽  
Normal Individuals ◽  
New Variant ◽  
Functional Defect ◽  
Von Willebrand ◽  
Willebrand Factor

Abstract We previously reported a functional defect of von Willebrand factor (vWF) in a new variant of von Willebrand disease (vWD) tentatively named vWD “Normandy.” The present work has attempted to characterize the molecular abnormality of this vWF that fails to bind factor VIII (FVIII). The immunopurified vWF from normal and patient's plasma were digested by trypsin and the resulting peptides were compared. The electrophoresis of ““vWF Normandy” showed a shift in the band corresponding to a polypeptide from amino acid 1 to 272. Consequently, we performed the molecular analysis of the portion of the vWF gene of this patient encoding this amino acid sequence. Exons 18–24 were amplified by the use of polymerase chain reaction and their nucleotide sequences corresponding to 1.8 kb were determined. Our analysis showed a point mutation C to T at codon 791, resulting in the substitution of Methionine for Threonine at position 28 of the mature vWF subunit. Because this nucleotide substitution destroyed a Mae II restriction site, this mutation was conveniently sought in various individual DNAs. The patterns obtained were consistent with the homozygous and heterozygous state of this mutation in the patient and in her son, respectively, and with its absence in 28 normal individuals. We conclude that Threonine at position 28 in plasma vWF may be crucial for the conformation and FVIII-binding capacity of its cystine-rich N- terminal domain.

Heparin Induced Release of Low Affinity Platelet Factor Four

1979 ◽  
Author(s):  
A.K. Rao ◽  
P. James ◽  
S. Niewiarowski
Keyword(s):  
Important Implication ◽  
Specific Protein ◽  
Final Concentration ◽  
Antigenic Determinants ◽  
Platelet Factor ◽  
Normal Individuals ◽  
The Mean ◽  
Minute Sample

Heparin has been reported to effect platelet function. We studied the effect of commercial beef lung heparin on the release of a platelet specific protein, the low affinity platelet factor 4(LA-PF4) in vitro and vivo. LA-PF4 shares Common antigenic determinants with B thromboglobulin, LA-PF4 was measured in platelet poor plasma (PPP) by radioimmunoassay. The mean levels of LA-PF4 in PPP from citrated blood and citrated blood with added heparin (final concentration 1.5 unit/ml) were 78.3±18.3 and 242.5±117.1 ng/ml respectively. (Mean ± SD, p <0.01). Addition of heparin to blood collected in a mixture of E EDTA, prostaglandin El, and theophylline did not result in an increase in LA-PF4 in PPP. A single intravenous bolus of heparin was administered to two normal individuals and the levels of LA-PF4 measured before and at 15,30 and 180 minutes later. An increase in the levels of LA-PF4 in the PPP from a baseline of 66.7 and 30.2 ng/ml/106 platelets to 166.7 and 167.4 ng/ml/106 platelets respectively were noted in 30 minute sample. There was no increase in LA-PF4 at 15 minutes. At 180 minutes levels were at baseline. It is concluded that heparin stimulates release of LA-PF4 from platelets both in vitro and in vivo. LA-PF4 has antiheparin action and is being investigated as a marker for thrombosis. Its release by heparin may, therefore, have an important implication.

Albumin as marker for susceptibility to metal ions in metal-on-metal hip prosthesis patients

2016 ◽  
Vol 36 (4) ◽  
pp. 319-327 ◽  
Author(s):  
F Facchin ◽  
S Catalani ◽  
E Bianconi ◽  
D De Pasquale ◽  
S Stea ◽  
...  
Keyword(s):  
Health Status ◽  
Blood Serum ◽  
Whole Blood ◽  
Metal Binding ◽  
Hip Prosthesis ◽  
Binding Capacity ◽  
Gene Mutations ◽  
Individual Susceptibility ◽  
Metal On Metal ◽  
Serum And Urine

Metal-on-metal (MoM) hip prostheses are known to release chromium and cobalt (Co), which negatively affect the health status, leading to prosthesis explant. Albumin (ALB) is the main serum protein-binding divalent transition metals. Its binding capacity can be affected by gene mutations or modification of the protein N-terminal region, giving the ischaemia-modified albumin (IMA). This study evaluated ALB, at gene and protein level, as marker of individual susceptibility to Co in MoM patients, to understand whether it could be responsible for the different management of this ion. Co was measured in whole blood, serum and urine of 40 MoM patients. A mutational screening of ALB was performed to detect links between mutations and metal binding. Finally, serum concentration of total ALB and IMA were measured. Serum total ALB concentration was in the normal range for all patients. None of the subjects presented mutations in the investigated gene. Whole blood, serum and urine Co did not correlate with serum total ALB or IMA, although IMA was above the normal limit in most subjects. The individual susceptibility is very important for patients’ health status. Despite the limited results of this study, we provide indications on possible future investigations on the toxicological response to Co.

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