scholarly journals Deposition of Apatite on Carbon Nanofibers in Simulated Body Fluid

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Seiichi Taruta ◽  
Kazuki Kidokoro ◽  
Tomohiko Yamakami ◽  
Tomohiro Yamaguchi ◽  
Kunio Kitajima ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Simulated Body Fluid ◽  
Carbon Nanofibers ◽  
Body Fluid ◽  
Hydroxyl Groups ◽  
Ion Concentrations ◽  
Inorganic Ion ◽  
Nucleation Sites ◽  
Concentrated Sulfuric Acid ◽  
Critical Nuclei

Carbon nanofibers (CNFs) were soaked in 1.5 simulated body fluid (1.5 SBF) in which inorganic ion concentrations are 1.5 times as high as those in the standard SBF. The influence of the CNFs content in 1.5 SBF and pretreatment of the CNFs on the biomimetical deposition of apatite were investigated. The spherical bone-like apatite particles were deposited on the pristine CNFs soaked in 1.5 SBF. Amount of deposited apatite per a unit of CNFs increased with a decrease in the CNFs content in 1.5 SBF, and it decreased markedly when the CNFs were pretreated with concentrated sulfuric acid/nitric acid (3 : 1 v/v) mixture for longer periods. Such results suggest that too many nucleation sites of apatite, which were functional groups, such as carboxyl and hydroxyl groups, existed on the CNFs in the 1.5 SBF, and most embryos formed on the sites could not grow to critical nuclei and furthermore did not grow to apatite.

Apatite Deposition on Polypeptides Derived from Plants in a Solution Mimicking Body Fluid

2006 ◽  
Vol 309-311 ◽  
pp. 671-674
Author(s):  
Toshiki Miyazaki ◽  
Chikara Ohtsuki ◽  
Shinichi Ogata ◽  
Masahiro Ashizuka
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Mechanical Performance ◽  
Extracellular Fluid ◽  
Organic Polymer ◽  
Carboxyl Groups ◽  
Organic Substrates ◽  
Ion Concentrations ◽  
Inorganic Ion ◽  
Apatite Deposition

Organic-inorganic hybrids composed of organic polymer and apatite is quite attractive as novel bone-repairing materials since it has mechanical performance analogous to those of natural bone as well as bone-bonding ability, i.e. bioactivity. To fabricate such an apatite-polymer hybrid, biomimetic process has been recently paid much attention. In this process, bone-like apatite is deposited on the surfaces of organic substrates in simulated body fluid (SBF, Kokubo solution) having ion concentrations analogous to those of human extracellular fluid or more concentrated solutions. Previous studies showed that the apatite deposition is triggered by a catalytic effect of carboxyl groups (COOH) on the surfaces of the organic substrates. In this study, we examined apatite deposition on natural polypeptides derived from crops in a biomimetic solution. We selected gluten derived from wheat and zein derived from corn. Both of gluten and zein formed bone-like apatite on their surfaces in a solution that has inorganic ion concentrations 1.5 times those of simulated body fluid, when they were treated with 1 mol/L calcium chloride solution. High content of acidic amino acids such as glutamic acid and aspartic acid in gluten and zein would give large amount of carboxyl groups effective for the apatite nucleation.

Effect of Concentrated Sulfuric Acid-Etching on Apatite-Forming Ability of Alkaline-Treated Titanium

2005 ◽  
Vol 284-286 ◽  
pp. 525-530 ◽  
Author(s):  
Seiji Ban ◽  
Hiroshi Kono ◽  
Y. Iwaya ◽  
Akihiko Yuda ◽  
Yuichi Izumi
Keyword(s):  
Sulfuric Acid ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Pure Titanium ◽  
Sodium Titanate ◽  
Alkaline Treatment ◽  
Acid Etching ◽  
Commercially Pure Titanium ◽  
Commercially Pure ◽  
Concentrated Sulfuric Acid

Concentrated H2SO4 acid was applied to pretreatment for the alkaline treatment of commercially pure titanium, and the effect of acid-etching on apatite-forming ability of alkaline-treated titanium in a simulated body fluid (SBF) was investigated. Characterization analysis revealed that the concentrated H2SO4 etching formed much amount of sodium titanate, resulting a large amount of formation of apatite in SBF. It is confirmed that the etching in concentrated H2SO4 enhance apatite-forming ability of alkaline-treated titanium.

scholarly journals Evaluation of Photocatalytic and Protein Adsorption Properties of Anodized Titanium Plate Immersed in Simulated Body Fluid

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Ryoji Sawada ◽  
Yuya Katou ◽  
Hirofumi Shibata ◽  
Max Katayama ◽  
Toru Nonami
Keyword(s):  
Sulfuric Acid ◽  
Simulated Body Fluid ◽  
Oxide Film ◽  
Protein Adsorption ◽  
Titanium Oxide ◽  
Body Fluid ◽  
Titanium Plate ◽  
Titanium Oxide Film ◽  
Adsorption Ability ◽  
Anodized Titanium

Titanium-based materials are widely used for implant treatments such as artificial dental roots. Surface treatment has the potential to improve not only the biocompatibility but also the chemical and mechanical durability of the surface without changing the mechanical properties of the metal. A relatively thick titanium oxide film can be formed by the anodic oxidation method. Phosphoric acid or sulfuric acid electrolytic solution has previously been used for anodic oxidation. Such anodized films have excellent film hardness, abrasion resistance, and adhesion. In this study, titanium plate was anodized using an aqueous solution of sulfuric acid in which titanium oxide powder was suspended. A 2800-nm-thick titanium oxide film was formed, which was thicker than that obtained using phosphoric acid electrolyte. The titanium plate was immersed in simulated body fluid for 1 day to evaluate the photocatalytic activity and protein adsorption ability, and a homogeneous crack-free hydroxyapatite layer was formed. This titanium plate showed high methylene blue bleaching capacity. The adsorption ability of the acidic protein of the anodized titanium plate subjected to the above treatment was high. This suggests that this titanium plate has antimicrobial properties and protein adsorption ability. Thus, we report that a titanium plate, anodized with a sulfuric acid aqueous electrolyte solution containing suspended TiO2 powder and immersed in simulated body fluid, might behave as an antibacterial and highly biocompatible implant material.

Comparison of Apatite Formation on Polyamide Films Containing Carboxyl and Sulfonic Groups in a Solution Mimicking Body Fluid

2006 ◽  
Vol 309-311 ◽  
pp. 477-480
Author(s):  
Chikara Ohtsuki ◽  
Takahiro Kawai ◽  
Masanobu Kamitakahara ◽  
Masao Tanihara ◽  
Toshiki Miyazaki ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Crystal Growth ◽  
Simulated Body Fluid ◽  
Calcium Ions ◽  
Body Fluid ◽  
Functional Group ◽  
Carboxyl Groups ◽  
Apatite Formation ◽  
Ion Concentrations ◽  
Polyamide Film

Apatite formation on polyamide films containing either carboxyl or sulfonic groups was compared in 1.5SBF, whose ion concentrations are 1.5 times those of a simulated body fluid (SBF). The sulfonic groups induced the apatite nucleation earlier than the carboxyl groups. In contrast, the rate of crystal growth depended not on the kind of functional group, but on the degree of supersaturation of the surrounding solution. The more ready association of sulfonic groups with calcium ions may lead to earlier apatite nucleation than that of carboxyl groups. Adhesive strength of the apatite layer to polyamide film containing sulfonic groups was significantly lower than that with carboxyl groups depending on the chemical interactions as well as on the mechanical properties of the polyamide film.

Development of Bioactive Apatite Nuclei-Precipitated Ti-12Ta-9Nb-6Zr-3V-O Alloy

2019 ◽  
Vol 829 ◽  
pp. 125-130
Author(s):  
Takeshi Yabutsuka ◽  
Yasutaka Kidokoro ◽  
Shigeomi Takai ◽  
Takeshi Yao
Keyword(s):  
Titanium Alloy ◽  
Sulfuric Acid ◽  
Simulated Body Fluid ◽  
Shape Memory Alloys ◽  
Shape Memory ◽  
Body Fluid ◽  
Physiological Condition ◽  
Roughened Surface ◽  
Conventional Titanium Alloy ◽  
Hydroxyapatite Formation

Ti-12Ta-9Nb-6Zr-3V-O alloy, one of the shape-memory alloys with lower Young’s modulus in comparison with conventional titanium alloy, was treated with sulfuric acid to form roughened surface on the substrate. In order to impart hydroxyapatite formation ability to the Ti-12Ta-9Nb-6Zr-3V-O alloy, apatite nuclei (AN) were precipitated on the roughened surface using simulated body fluid (SBF) adjusted at higher pH than physiological condition. By this treatment, AN-precipitated Ti-12Ta-9Nb-6Zr-3V-O alloy was obtained. The AN-precipitated Ti-12Ta-9Nb-6Zr-3V-O alloy showed high hydroxyapatite formation ability in physiological SBF.

Microstructure and In Vitro Bioactivities of HA/Ti Composites Prepared by Hot Isostatic Pressing

2007 ◽  
Vol 336-338 ◽  
pp. 1715-1717
Author(s):  
Hua Ke ◽  
Yu Zhou ◽  
De Chang Jia ◽  
Cong Qin Ning
Keyword(s):  
Simulated Body Fluid ◽  
Human Plasma ◽  
Body Fluid ◽  
Ph Value ◽  
Hot Isostatic Pressing ◽  
Biological Properties ◽  
Isostatic Pressing ◽  
Ion Concentrations

Hydroxyapatite (HA)/titanium (Ti) composites were successfully fabricated by hot isostatic pressing at 850°C. The microstructure of 7T2HB (70Ti+20HA+10Bioglass, Vol. %) composites were systematically investigated. The main constituents of the composites are hydroxyapatite and titanium. A simulated body fluid (SBF) with the same ion concentrations as those of human plasma and pH value of 7.4 is selected to evaluate the in vitro biological properties of the composites. After 2-week immersion in SBF, the bioactive apatite formed a very dense film on the surface of the composites. The dissolution of CaO and Ca-P compounds in SBF improves the growth of apatite on the surface of the composites.

Mineralization of phosphorylated cellulose/ sodium alginate sponges as biomaterials for bone tissue engineering

2021 ◽  
Author(s):  
li Zha ◽  
Yahui Zheng ◽  
Jianfei Che ◽  
Yinghong Xiao
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Sodium Alginate ◽  
Body Fluid ◽  
Hydroxyl Groups ◽  
Cellulose Sponge

In recent years cellulose sponge is attractive for bone tissue materials in tissue engineering, however cellulose with hydroxyl groups shows weak apatite nucleation ability in simulated body fluid (SBF). In...

Functionalized Copolymers and Their Composites with Polylactide And Hydroxyapatite

1998 ◽  
Vol 550 ◽  
Author(s):  
S. Jin ◽  
K. E. Gonsalves
Keyword(s):  
Simulated Body Fluid ◽  
Functional Groups ◽  
Body Fluid ◽  
Inorganic Composites ◽  
Nucleation Sites ◽  
Vinylphosphonic Acid

AbstractSynthetic copolymers poly(E-caprolactone-co-vinylphosphonic acid) (P(MDOVPA) and poly(E-caprolactone-co-dimethylvinylphosphoester) (P(MDOVPE)) were used to prepare composites with polylactide (PLac) and hydroxyapatite (HAp). The P(MDOVPA) was used as filler in PLac films, as it has pendant functional groups P(O)(OH)2, providing nucleation sites for the deposition of HAp in simulated body fluid. HAp growth on PLac-P(MDOVPA) film was observed by XRD. The incorporation of hydrophilic P(MDOVPE) into PLac increased the hydrophilicity of the blend. Synthetic HAp was also used to make multi-layered, alternating organic-inorganic composites with porous PLac-P(MDOVPE) blends.

Biomimetic Process in Metals

2006 ◽  
Vol 509 ◽  
pp. 211-0 ◽  
Author(s):  
Marco A. Lopez-Heredia ◽  
Dora A. Cortés-Hernández ◽  
José C. Escobedo-Bocardo ◽  
A. Medina Ramírez
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Base Alloy ◽  
Ionic Concentration ◽  
Apatite Layer ◽  
Human Blood Plasma ◽  
Ion Concentrations ◽  
Heat Treated ◽  
Cobalt Base Alloy ◽  
Cobalt Base

A titanium base alloy and a cobalt base alloy have been subjected to a biomimetic process. Samples of titanium and cobalt alloys have been immersed in 10M and 5M NaOH solutions, respectively, then the samples have been heat treated and finally, immersed in either a simulated body fluid (SBF) with ionic concentration close to human blood plasma or in a simulated body fluid with an ionic concentration 1.5 times that of the SBF (1.5SBF) for a period of 21 days. An apatite layer has been observed after the immersion of the samples in SBF for both Ti and Co base alloys. The apatite layer observed on the Ti alloy samples is more homogeneous and thicker than that observed on the Co alloy samples. However, the apatite layer on both samples is not continuous and homogeneous along the surface. The layer of apatite formed is thicker on samples immersed in 1.5SBF. This is attributed to the higher ion concentrations, mainly of calcium and phosphorus. The Ca/P ratios measured in the apatite layers are close to that of bone.

Sign in / Sign up

Export Citation Format

Share Document