scholarly journals Characterization of the Nigerian Population of the reniform nematode, Rotylenchulus reniformis based on the Analysis of the 28S ribonucleic DNA

2020 ◽  
Keyword(s):  
Reniform Nematode ◽  
Rotylenchulus Reniformis ◽  
Nigerian Population

scholarly journals Quantitative Trait Loci Associated with Rotylenchulus reniformis Host Suitability in Soybean

2020 ◽  
Vol 110 (9) ◽  
pp. 1511-1521
Author(s):  
Juliet Wilkes ◽  
Christopher Saski ◽  
Mariola Klepadlo ◽  
Benjamin Fallen ◽  
Paula Agudelo
Keyword(s):  
Quantitative Trait Loci ◽  
Qtl Analysis ◽  
Quantitative Trait ◽  
The United States ◽  
Host Suitability ◽  
Reniform Nematode ◽  
Rotylenchulus Reniformis ◽  
Chromosome 18 ◽  
Nematode Reproduction ◽  
Trait Loci

Reniform nematode (Rotylenchulus reniformis) is a yield-limiting pathogen of soybean (Glycine max) in the southeastern region of the United States. A population of 250 recombinant inbred lines (RIL) (F2:8) developed from a cross between reniform nematode resistant soybean cultivar Forrest and susceptible cultivar Williams 82 was utilized to identify regions associated with host suitability. A genetic linkage map was constructed using single-nucleotide polymorphism markers generated by genotyping-by-sequencing. The phenotype was measured in the RIL population and resistance was characterized using normalized and transformed nematode reproduction indices in an optimal univariate cluster analysis. Quantitative trait loci (QTL) analysis using normalized phenotype scores identified two QTLs on each arm of chromosome 18 (rrn-1 and rrn-2). The same QTL analysis performed with log10(x) transformed phenotype data also identified two QTLs: one on chromosome 18 overlapping the same region in the other analysis (rrn-1), and one on chromosome 11 (rrn-3). While rrn-1 and rrn-3 have been reported associated with reduced reproduction of reniform nematode, this is the first report of the rrn-2 region associated with host suitability to reniform nematode. The resistant parent allele at rrn-2 showed an inverse relationship with the resistance phenotype, correlating with an increase in nematode reproduction or host suitability. Several candidate genes within these regions corresponded with host plant defense systems. Interestingly, a characteristic pathogen resistance gene with a leucine-rich repeat was discovered within rrn-2. These genetic markers can be used by soybean breeders in marker-assisted selection to develop lines with resistance to reniform nematode.

scholarly journals First Report of the Reniform Nematode (Rotylenchulus reniformis) from Soybean in Paraguay

Plant Disease ◽  
2018 ◽  
Vol 102 (10) ◽  
pp. 2043-2043
Author(s):  
H. D. Lopez-Nicora ◽  
L. M. Pedrozo ◽  
C. Grabowski Ocampos ◽  
A. L. Orrego Fuente ◽  
E. Hahn Villalba ◽  
...  
Keyword(s):  
Reniform Nematode ◽  
Rotylenchulus Reniformis ◽  
First Report

scholarly journals Genetic Variability of Rotylenchulus reniformis

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Megan Leach ◽  
Paula Agudelo ◽  
Amy Lawton-Rauh
Keyword(s):  
United States ◽  
Genetic Variation ◽  
Southeastern United States ◽  
Genetic Connectivity ◽  
Group Method ◽  
Reniform Nematode ◽  
Rotylenchulus Reniformis ◽  
Pair Group ◽  
High Level ◽  
The Impact

Rotylenchulus reniformis, reniform nematode, is a polyphagous pest commonly found parasitizing cotton in the southeastern United States. We developed and optimized 10 polymorphic microsatellite loci found in reniform nematode and tested them on 160 individual reniform nematodes to determine informative genetic variation of isolates from the southeastern United States, Colombia, Japan, and from the species Rotylenchulus parvus. No significant gametic disequilibrium was detected between any pair of loci, and most loci were not in expected Hardy-Weinberg proportions. A positive FIS coefficient was observed at all 10 loci, suggesting a high level of inbreeding at these loci. Most isolate locations exhibited significant genotypic differentiation and moderate to very high genetic differentiation based on FST analysis. The most consistently differentiated isolates were found reproducing parthenogenetically in Japan. These isolates were also found to represent the most basal locality in this study based on unweighted pair group method with arithmetic mean (UPGMA) clustering analysis and were distinct from other localities based on STRUCTURE V 2.3 analysis. These results support previous reports suggesting that the parthenogenetically reproducing isolates from Japan are another species. Taken together, our results can serve as the foundation for more extensive characterization of population structure and genetic variation among isolates of R. reniformis variants to help discern the impact of alternative processes on genetic connectivity and differentiation in the genetically undercharacterized reniform nematode.

scholarly journals Conventional PCR Detection and Real-Time PCR Quantification of Reniform Nematodes

Plant Disease ◽  
2012 ◽  
Vol 96 (12) ◽  
pp. 1757-1762 ◽  
Author(s):  
Ronald J. Sayler ◽  
Courtney Walker ◽  
Fiona Goggin ◽  
Paula Agudelo ◽  
Terrence Kirkpatrick
Keyword(s):  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Its Region ◽  
Taqman Probe ◽  
Reniform Nematode ◽  
Rotylenchulus Reniformis ◽  
Conventional Pcr ◽  
Diagnostic Assays ◽  
Reniform Nematodes

Reniform nematode (Rotylenchulus reniformis) is a relatively recent introduction into the continental United States that can cause major yield losses on a variety of important crops including cotton and soybeans. DNA sequences from the internal transcribed spacer (ITS) region of this nematode were used to design primers for conventional and real-time PCR, as well as a TaqMan probe. These primers amplified DNA of reniform nematode isolates from a wide geographic range but did not detect genetically related species or other pathogenic nematodes found in production fields including Meloidogyne incognita and Heterodera glycines. Both SYBR green and TaqMan assays reliably quantified as little as 100 fg of reniform nematode DNA, and could be used to quantify as few as five reniform nematodes. An inexpensive and rapid DNA extraction protocol for high throughput diagnostic assays is described.

scholarly journals Descriptive characterization of a nigerian heterogeneous rabbit population - factors affecting litter traits.

2010 ◽  
Vol 18 (2) ◽  
pp. 111
Author(s):  
S.O. Oseni ◽  
B.A. Ajayi
Keyword(s):  
Litter Size ◽  
Reproductive Traits ◽  
Daily Weight Gain ◽  
Average Weight ◽  
Font Size ◽  
Factors Affecting ◽  
Litter Traits ◽  
Nigerian Population ◽  
Size At Birth

<span style="font-size: small;"><span style="font-size: 13px; line-height: 14px;">Thi study evaluated the effect of female body weight at conception (FWC), season of mating (SM), type of mating (TM) and litter size at birth (LSB) on the litter performance in a heterogeneous Nigerian population of rabbits. Data on 116 litters from 10 bucks and 48 does across three parities were analysed. Reproductive traits evaluated included LSB, number born alive (NBA), litter sizes and weights at 7, 14 and 21 d post-kindling, pre-weaning survival rate (SR) and daily weight gain of kits from kindling to weaning (DWG). A fi xed linear model that included FWC (light and heavy does), TM (homospermic and heterospermic matings), LSB classes (low, medium and large) and SM (rainy or dry) was used. Results showed that heavier does at concenption showed higher performance for LSB and NBA (P&lt;0.05) than lighter does. Average weight of kits at kindling and at 28 d, as well as kit SR and DWG, were signifi cantly higher in low-sized litters, when compared with intermediate- and large-sized litters (P&lt;0.05). LSB, NBA and litter size at weaning were higher in litters produced by heterospermically mated does compared with homospermically mated does. Season of mating affected LSB and NBA (P&lt;0.05), being signifi cantly larger the litters in the rainy season.</span></span>

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