scholarly journals A Comparison of Osteoblast Cell Proliferation and Osteocalcin Expression in Cuttlefish Bone and Bovine Bone Xenograft

2019 ◽  
Vol 3 (2) ◽  
pp. 75 ◽  
Author(s):  
Komang Agung Irianto ◽  
Ameria Pribadi ◽  
Ilham Abdullah Irsyam ◽  
Yudhistira Pradnyan Kloping ◽  
Oen Sindrawati
Keyword(s):  
Cell Proliferation ◽  
Sem Analysis ◽  
Bovine Bone ◽  
Osteoblast Cell ◽  
X Ray Diffraction ◽  
X Ray ◽  
Good Potential ◽  
Fouriertransform Infrared Spectroscopy ◽  
And Control ◽  
Made In

Background: Cuttlefish bone Xenograft, calcium phosphate (CaP)-based biomaterial graft, offers an alternative and has been accepted for osteoconductive and probable osteo-inductive attributes. This study aims to compare the bone healing potential between the bovine-derived (BHA) and cuttlefish bone-derived (CHA).Materials and Methods: The study compared osteoblast cell proliferation of 27 New Zealand rabbits in 2.5 mm bone defect made in the femoral bone. The samples were divided into three groups, which were control, BHA and CHA group. The chemical and physical characteristics of BHA and CHA were determined for the content of hydroxyapatite by FourierTransform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD), then tested by Scanning Electron Microscopy (SEM) to evaluate the porosity. In the end of the second week, histopathologic and immunohistochemistry examinations were performed to evaluate the amount of osteoblast and osteocalcin expression.Results: The FTIR, XRD and SEM analysis showed both BHA and CHA samples were hydroxyapatite according to Joint Committee on Powder Diffraction Standards (JCPDS). The CHA was significantly higher (297.22±19.772) compared to BHA (258.22±30.926) and control (131.67±34.213). Osteocalcin expression in CHA (7.82±2.230) compared to BHA (6.09±3.724) and control (4.07±3.606), was not significant (p>0.05). Conclusion: CHA group has the highest osteoblast cell proliferation and osteocalcin expression, meaning has a good potential as future source of bone graft.Keywords: cuttlefish bone, bovine, bone graft, osteoblast cell

scholarly journals Changes in mandible due to osteoporosis

2007 ◽  
Vol 54 (1) ◽  
pp. 16-27 ◽  
Author(s):  
Srdjan Postic
Keyword(s):  
Calcium Phosphate ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Mandibular Bone ◽  
X Ray ◽  
Noticeable Increase ◽  
Edentulous Mandible ◽  
Bone Structures ◽  
Inorganic Constituents ◽  
And Control

Introduction: Osteoporosis may occur in bone tissue as a result of ageing. Aim: The aim of this study was to investigate remodeling of old human edentulous mandibular bone due to osteoporosis. Materials and Methods: Osseous surfaces of one edentulous and one dentate mandible were extracted from a cadaver. This study included 5 methods of analysis of bone structures in experimental and control bony samples of human mandibular bones. Absolute densities of samples were determined. SEM analysis and X-ray diffraction analysis of spectra were used to assess bony layers. Results: Minimum density 1.9658 g/cm3 of edentulous ridge site was detected. SEM micrographs indicated reductions of trabecular and lamellar width. Significant differences (p<0.01) in cancellous site width were recorded in experimental basal specimens. Noticeable increase of porosity was observed in 6.35% of edentulous ridge site and 14.06% of experimental basal site. There was a significant correlation between oxygen presence (p=0.046) eroded and defect bone surfaces (p<0.001). Complexes of hydroxyapatite, calcium-phosphate, tetracalcium-pyrophosphate and amorphous calcium-phosphate were inorganic constituents considerable mineral modifications of edentulous ridge site. Conclusion. Based on the results of the present study, various levels of degradation of cortical matrix and cancellous layers of edentulous mandible may be observed as a result of osteoporosis. .

Development of Ternary Concrete Utilizing Agricultural Waste Material

2022 ◽  
Author(s):  
Sunita Kumari ◽  
Dhirendra Singhal ◽  
Rinku Walia ◽  
Ajay Rathee
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Agricultural Waste ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Concrete Mix ◽  
Maize Cob

Abstract The present project proposes to utilize rice husk and maize cob husk ash in the cement to mitigate the adverse impact of cement on environment and to enhance the disposal of waste in a sustainable manner. Ternary concrete / MR concrete was prepared by using rise husk and maize cob ash with cement. For the present project, five concrete mixes MR-0 (Control mix), MR-1 (Rice husk ash 10% and MR-2.5%), MR-2 (Rice husk ash 10% and MR-5%), MR-3 (Rice husk ash 10% and MR-2.5%), MR-4 (Rice husk ash 10% and MR-2.5%) were prepared. M35 concrete mix was designed as per IS 10262:2009 for low slump values 0-25mm. The purpose is to find the optimum replacement level of cement in M35 grade ternary concrete for I – Shaped paver blocks.In order to study the effects of these additions, micro-structural and structural properties test of concretes have been conducted. The crystalline properties of control mix and modified concrete are analyzed by Fourier Transform Infrared Spectroscope (FTIR), Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD). The results indicated that 10% Rice husk ash and 5% maize cob ash replaced with cement produce a desirable quality of ternary concrete mix having good compressive strength. The results of SEM analysis indicated that the morphology of both concrete were different, showing porous structure at 7 days age and become unsymmetrical with the addition of ashes. After 28 day age, the control mix contained more quantity of ettringite and became denser than ternary concrete. XRD analysis revealed the presence of portlandite in large quantity in controlled mix concrete while MR concrete had the partially hydrated particle of alite.

scholarly journals Extraction of γ-Alumina from Low-Cost Kaolin

Resources ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 63
Author(s):  
Khalil Ibrahim ◽  
Mohammad Moumani ◽  
Salsabeela Mohammad
Keyword(s):  
Low Cost ◽  
Morphological Properties ◽  
X Ray Diffraction ◽  
X Ray ◽  
Aluminum Metal ◽  
Alumina Particles ◽  
Two Stages ◽  
Method Of Extraction ◽  
Microcrystalline Powder ◽  
Made In

A combined process is proposed for the utilization of local kaolin to produce alumina particles. The applied process is made in two stages: calcination at 700 °C with sodium chloride and leaching with sulfuric followed by hydrochloric acids. The optimal extraction efficiency can be obtained when the conditions are as follows: leaching temperature is at 140 °C, leaching time is 3 h 45 min and concentration of sulfuric acid is 40 wt.%. The results show that the purity of alumina reaches 79.28%, which is suitable for the production of aluminum metal. It is evident that this method of extraction of alumina from the kaolin ash is practical and feasible. The structural and morphological properties of the calcined microcrystalline powder was characterized by X-ray diffraction and scanning electron microscope (SEM).

scholarly journals Controlled sample environment for studying solid–gas interactions by in situ powder X-ray diffraction

2021 ◽  
Vol 54 (1) ◽  
pp. 371-375
Author(s):  
Paul Monceyron Røren ◽  
Kristoffer W. B. Hunvik ◽  
Vegard Josvanger ◽  
Ole Tore Buseth ◽  
Jon Otto Fossum
Keyword(s):  
Applied Pressure ◽  
Copper Plate ◽  
Temperature Control System ◽  
X Ray Diffraction ◽  
X Ray ◽  
Custom Made ◽  
Quartz Capillary ◽  
Gas Interactions ◽  
And Control

A sample cell for powder X-ray diffraction studies with in situ applied pressure and control of temperature is demonstrated. The cell is based on a previously reported design and consists of a glass or quartz capillary glued into a Swagelok weld gland; this configuration can hold up to 100 bar (1 bar = 100 kPa). The cell is placed in contact with a copper plate for control of temperature between −30 and 200°C. This is achieved by Peltier elements, heat cartridges and a refrigerated circulating bath. This work mainly focuses on the temperature control system. Commissioning tests were performed in a custom-made small/wide-angle X-ray diffractometer at the Norwegian University of Science and Technology. The system is easily portable to synchrotron facilities.

scholarly journals Effect of Re and Tm-site on morphology structure and optical band gap of ReTmO3 (Re: La, Ce, Nd, Gd, Dy, Y and Tm: Fe, Cr) prepared by sol-gel method

2018 ◽  
Vol 64 (4) ◽  
pp. 381
Author(s):  
Muhammad Tufiq Jamil ◽  
Javed Ahmad ◽  
Syed Hamad Bukhari ◽  
Murtaza Saleem
Keyword(s):  
Band Gap ◽  
Sol Gel ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
Orthorhombic Crystal ◽  
X Ray ◽  
Average Grain Size ◽  
Auto Combustion ◽  
Uv Visible

Rare earth nano sized pollycrystalline orthoferrites and orthocromites ReT mO3 (Re = La, Nd, Gd, Dy, Y and T m = Fe, Cr) have been synthesized by sol-gel auto combustion citrate method. The samples have been characterized by means of X-ray diffraction (XRD), scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), and UV-visible spectroscopy. The samples are single phase as confirmed by XRD analysis and correspond to the orthorhombic crystal symmetry with space group pbnm. Debye Scherer formula and Williamson Hall analysis have been used to calculate the average grain size which is consistent with that of determined from SEM analysis and varied between 25-75 nm. The elemental compositions of all samples have been checked by EDX analysis. Different crystallographic parameters are calculated with strong structural correlation among Re and Tm sites. The optical energy band gap has been calculated by using Tauc relation estimated to be in the range of 1.77 - 1.87 eV and 2.77 - 3.14 eV, for ReFeO3 and ReCrO3, respectively.

Standardisation of X-Ray Powder Diffraction Methods

2004 ◽  
Vol 443-444 ◽  
pp. 31-34
Author(s):  
Giovanni Berti ◽  
Rob Delhez ◽  
S. Norval ◽  
B. Peplinski ◽  
E. Tolle ◽  
...  
Keyword(s):  
Technical Committee ◽  
Non Destructive Testing ◽  
X Ray Diffraction ◽  
Destructive Testing ◽  
X Ray ◽  
Standard Documents ◽  
And Control ◽  
Non Destructive ◽  
Research Centres

This paper outlines the standardisation process for the XRPD method that is currently being considered by a Working Group (WG10) of Technical Committee 138 "Non-destructive Testing" of the European Committee for Standardisation CEN. Several Standard Documents are on the verge of being released. These documents concern the general principles of (X-ray) diffraction, its terminology, and the basic procedures applied. Another document concerns the instruments used and it offers procedures to characterise and control the performance of an X-ray diffractometer properly. It is intended to issue Standard Documents on specific methods, e.g. determination of residual stresses. In fact work is in progress on this subject. The Standard Documents can be used by industry, government organisations, and research centres with activities related to safety, health and the environment, as well as for educational purposes.

Hierarchical Nanostructured ZnO-CuO Nanocomposite and its Photocatalytic Activity

2015 ◽  
Vol 35 ◽  
pp. 21-26 ◽  
Author(s):  
Susmita Das ◽  
Vimal Chandra Srivastava
Keyword(s):  
Photocatalytic Activity ◽  
Pure Copper ◽  
Ultra Violet ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ultra Violet Radiation ◽  
One Step ◽  
Uv Visible

Metal oxide nanocomposite (ZnO-CuO) was successfully synthesized by one step homogeneous coprecipitation method and further characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron micrograph (SEM), X-ray diffraction analysis (XRD) and UV-visible diffuse reflectance spectra. XRD analysis exhibited presence of pure copper oxide and zinc oxide within the nanocomposite. SEM analysis indicated that the ZnO-CuO nanocomposite was consisted of flower shaped ZnO along with leaf shaped CuO. Photocatalytic activity of nanocomposite was evaluated in terms of degradation of methylene blue (MB) dye solution under ultra-violet radiation. Results showed that the photocatalytic efficiency of ZnO-CuO nanocomposite was higher than its individual pure oxides (ZnO or CuO).

Using Ultrathin-Film, Modulated Composites to Control the Reaction Mechanism of Ternary Compound Formation

1991 ◽  
Vol 238 ◽  
Author(s):  
Loreli Fister ◽  
David C. Johnson
Keyword(s):  
Ternary Compound ◽  
Reaction Pathway ◽  
Experimental Parameter ◽  
Ultrathin Film ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Compound Formation ◽  
One Dimensional ◽  
X Ray ◽  
Made In

ABSTRACTA predictable, controllable approach to the synthesis of ternary compounds through known intermediates is presented. Thin and ultrathin film superlattices were made in the Mo-Se, Cu-Se and Mo-Cu systems. Differential scanning calorimetry, low- and high-angle x-ray diffraction were used to assess the interdiffusion and nucleation reactions between elemental layers in these one-dimensional crystals. The experimental parameter modulation distance was used to influence the interfacial reactions. The results from each binary system were then used to predict the reaction pathway in the synthesis of a ternary compound, Cu2Mo6Se8. Superlattices with two different lengthscales were investigated. In the first, only one intermediate, MoSe2 which typically crystallizes at ∼200'C, is observed prior to the crystallization of Cu2Mo6Se8. In the second, no crystalline intermediates are observed below 6000 C.

Wear Behavior of TiB2/GNPs and B4C/GNPs Reinforced AA6061 Matrix Composites

2021 ◽  
Vol 143 (11) ◽  
Author(s):  
Safa Polat ◽  
Yavuz Sun ◽  
Engin C¸evik
Keyword(s):  
Wear Behavior ◽  
Sem Analysis ◽  
Matrix Composites ◽  
X Ray Diffraction ◽  
Pressure Infiltration ◽  
X Ray ◽  
Wear Rates ◽  
Graphene Nanoparticles ◽  
Infiltration Method ◽  
Scanning Electron

Abstract In this study, it was aimed to investigate the effects of reinforcements used for improving the thermal properties of AA6061 alloy on wear resistance. For this purpose, AA6061 matrix composites were produced by pressure infiltration method using ceramic microparticles (TiB2 and B4C) and graphene nanoparticles (GNPs). The produced composites were first characterized by porosity measurement, X-ray diffraction (XRD), and scanning electron microscopy (SEM) analysis. Then, the wear behavior was examined under three different loads (20–40–60 N) with the reciprocating ball on the flat method in a dry environment. Specific wear-rates were calculated according to the Archard principle by measuring the depth and width of the traces after tests with a profilometer. Wearing mechanisms were determined with the help of optical and microstructure images. According to the obtained results, it was found that B4C + GNPs reinforced samples were more resistant to abrasion at low loads, but TiB2 + GNPs reinforced samples were higher at higher loads.

Antibacterial Activity of Nickel -Doped ZnO\MWCNTs hybrid Prepared by Sol-Gel technique

2021 ◽  
Author(s):  
Selma M.H. AL-Jawad ◽  
Zahraa S. Shakir ◽  
Duha S. Ahmed
Keyword(s):  
Antibacterial Activity ◽  
Sol Gel ◽  
Inhibition Zone ◽  
Sem Analysis ◽  
Morphological Properties ◽  
X Ray Diffraction ◽  
Zno Nps ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Doped Zno

ZnO/MWCNTs hybrid and doped with different concentration of Nickel element prepared by using Sol-gel been technique reported. All samples were prepared and characterized by X-Ray Diffraction Analysis (XRD), Energy Dispersive X-ray Spectroscopy (EDS), Fourier-Transform Infrared Spectroscopy (FTIR), Field-Emission Scanning Electron Microscopy (FE-SEM), and UV-Vis spectroscopy have been identified the structural, optical and morphological properties. X-ray diffraction showed the polycrystalline nature with hexagonal wutzite structure of hybrid and doped with Nickel. The crystalline size of the hybrid nanostructure was increasing from 23.73 nm to 34.59 nm. Besides, the UV-Vis spectroscopy showed a significant decrease in the band gap values from 2.97 eV to 2.01 eV. Whereas the FE-SEM analysis confirm the formation spherical shapes of ZnO NPs deposited on cylindrical tubes representing the MWCNTs. The antibacterial activity reveals that the inhibition zone of Ni doped-ZnO/MWCNTs hybrid was 28.5 mm, 26.5 mm toward E. coli and S. aureus bacteria, respectively.

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