scholarly journals Isolation and Characterization of Streptomycetes with Potential to Decompose Organic Compounds During Bioremediation of Arable Soil

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Ekenwosu Joseph Ugochukwu ◽  
Peter Ugochukwu Okorie
Keyword(s):  
Escherichia Coli ◽  
Organic Compounds ◽  
Culture Media ◽  
Arable Soil ◽  
Bacillus Species ◽  
Klebsiella Species ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Pseudomonas Species ◽  
Enterobacter Species

The study has an objective of isolating and characterizing suspected Streptomycetes with the potential to decompose organic compounds in arable soil. The isolates were grown on a culture media and a total of 61 slopes were inoculated and labeled using the following characterization tests: catalase test, gram staining, oxidase test, motility test, and oxidative-fermentative test. After characterization tests, data generated in the laboratory were analyzed and the study showed that isolates A2 (c, e), B2 (c), C1 (b), C2 (a), D1 (e) and D2 (d) were suspected to be Streptomycetes species. Isolates D1 (d) and D2 (b) were suspected to be Escherichia coli while isolates A1 (a), B2 (a, b) and D1 (a) were suspected to be Bacillus species. Isolates A1 (b), C1 (a), D1 (c), D2 (e) were suspected to be Pseudomonas species. Further identification showed that isolates A2 (d) and B1 (b) could be Enterobacter species while isolates A2 (a, b) were suspected to be Klebsiella species. The study tentatively identified Streptomycetes species; Escherichia coli; Bacillus species; Enterobacter species; Pseudomonas species and Klebsiella species. The suspected Streptomycetes identified were considered as potential organic matter decomposers in arable soil.

scholarly journals Antimicrobial Susceptibility Profile of Urinary Tract Pathogenic Infections in Diabetic Patients Attending a Health Facility in Kumasi, Ghana

2020 ◽  
pp. 37-45
Author(s):  
Maxwell Adu-Poku ◽  
Matthew Glover Addo
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Health Facility ◽  
Antimicrobial Susceptibility ◽  
Diabetic Patients ◽  
Klebsiella Species ◽  
E Coli ◽  
Antimicrobial Susceptibility Profile ◽  
Pseudomonas Species ◽  
Proteus Species

Aims: This study seeks to investigate the antimicrobial susceptibility profile of urinary tract pathogenic infections in diabetic patients attending a health facility in Kumasi, Ghana. Study Design: A total of 285 patients were recruited using Cochran’s formula at a prevalence of 26.4% for this study from patients attending the University Hospital from April 2018 to October 2018. Data were collected using a structured questionnaire. Methodology: Clean-catch midstream urine samples were screened for the presence of pathogenic bacteria and their antimicrobial susceptibility pattern using recommended culture methods. Results: Out of the 285 patients, 125 (43.9%) were diabetic with 90 (72%) being female and 35 (28%) male. There was no association between UTI’s and gender (P=0.5799) with diabetic patients recording higher bacteriuria compared to non-diabetics (P< 0.001). Isolates from 113 (39.4%) of the samples were identified and these included, Escherichia coli, Pseudomonas species, Klebsiella species, Proteus species and Staphylococcus aureus. The most predominant was E. coli 62 (54.9%) followed by S. aureus 24 (21.2%), Klebsiella species 14(12.4%), Pseudomonas species 12(10.6%) and Proteus species 1(0.9%). E. coli showed a high antimicrobial sensitivity rates against most of the tested antibiotics, gentamycin (90.3%), amikacin (98.4%), nalidixic (34%), cefotaxime (80.6%) and nitrofurantoin (93%). Proteus spp. on the other hand, showed a 100% sensitivity to all the antibiotics except tetracycline, amikacin and cefotaxime. It was observed that Escherichia coli was mostly resistant to tetracycline (96.8%), norfloxacin (69.4%) and cefotaxime (61.4%). Conclusion: S. aureus showed a higher level of resistance to tetracycline (100%). The prevalence of this study indicated that UTI among diabetic patients was relatively comparable with other studies. Amikacin and nitrofurantoin should be recommended as antimicrobials for the treatment of UTIs whilst the use of tetracycline, norfloxacin and cefotaxime should be discouraged.

scholarly journals Bacteriological Evaluation of Some Automated Teller Machine In Akure Metropolis

2019 ◽  
Vol 3 (1) ◽  
pp. 183-187
Author(s):  
Iyabo A. Simon-Oke
Keyword(s):  
Pathogenic Bacteria ◽  
Bacillus Species ◽  
Identification System ◽  
Staining Reaction ◽  
Bacterial Isolates ◽  
Klebsiella Species ◽  
Bacteria Growth ◽  
Gram Staining ◽  
Bacterial Contaminants ◽  
Strip Test

Background: Automated Teller Machines (ATMs) represent a good transient environment for development of pathogenic microbes. Investigation on the level of bacterial contaminants on keypads of ATMs was carried out in Akure, Nigeria. Methods: A total of sixty ATMs keypads were sampled in two major locations (main market and Federal University of Technology, campus) in Akure. Samples were collected from key-pad and screen parts of the ATM devices with sterile swab sticks and was cultured immediately on Nutrient agar, Manitol salt agar, and MacConkey agar mediums for Microbial examination. Standard bacteriological methods were employed in the analysis of the sample. Presumptive identification of bacterial isolates was by cellular morphology, Gram staining reaction, motility, catalase, coagulase test, oxidase strip test and MICROBACT Biochemical Identification system was used to identify the species of the oxidase negative Gram negative bacteria. Results: Out of 30 ATMs keypads examined in the two locations, 28 and 22 yeilded bacteria growth in Akure main Market and FUTA Campus respectively. The organisms isolated were Staphylococcus aureus, Klebsiella species, Escherichia coli, Pseudomonas and Bacillus species. All the bacterial isolates showed high resistance to Ampicillin but low resistance to Ciprofloxacin. Conclusion: The study confirmed the presence of pathogenic bacteria species on ATM key-pads with possible health implications in Akure, Nigeria. Daily and regular cleaning regimen of the key-pads with sanitizers, and public enlightenment on the hygienic usage of the keypads is necessary to reduce health risks to the users.

scholarly journals Frequency and Distribution of Gram Negative Bacteria among Hospital and Community Acquired UTI Patients

2015 ◽  
Vol 1 (2) ◽  
pp. 24-26
Author(s):  
Md. Badrul Islam ◽  
Md. Abdullah Yusuf ◽  
Md. Shahjahan Chowdhury ◽  
AFM Arshaedi Sattar ◽  
Samia Afrin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Gram Negative Bacteria ◽  
Klebsiella Species ◽  
Gram Negative ◽  
Pseudomonas Species ◽  
Community Patients

Background: Gram negative bacteria create a great problem during the treatment of urinary tract infection patients. Objective: This study was undertaken to determine the frequency and distribution of Gram negative bacteria among the UTI patients. Methodology: This cross sectional study was carried out in the Department of Microbiology at Sir Salimullah Medical College, Dhaka from June 2007 to May 2008 for a period of 1(one) year. All the patients presented with the clinically suspected UTI at any age with both sexes were selected as study population. Patients who were hospitalized for at least 2 days or more received different antibiotics were designated as hospital acquired UTI; on the other hand patients who were attended in OPD for the first time were considered as community acquired UTI patients. All urine samples were inoculated in Blood agar and MacConkeys agar media. Gram negative bacteria were isolated and identified by colony morphology, Gram staining and biochemical tests. Result: A total of 220 urine samples were collected from patients suspected to urinary tract infections of which 116 samples were from hospitalized patients and 104 samples were from community patients. Among 220 samples, 132(60.0%) Gram negative bacteria were isolated of which 88(66.7%) isolates were from hospitalized acquired UTI and 44(33.3%) bacteria were isolated from community acquired UTI patients. The difference was statistically significant (p=0.0001). In hospitalized patients out of 88(75.9%) isolated Gram negative bacteria, 67(76.1%) isolates were Escherichia coli, 10(11.4%) isolates were Klebsiella species, 5(5.7%) isolates were Proteus species and 6(6.8%) isolates were Pseudomonas species. Among the isolated bacteria 44(42.3%) bacterial isolates were from community patients of which 36(81.8%) isolates were Escherichia coli, 4(9.1 %) isolates were Klebsiella species, 2(4.5%) isolates were Proteus species and 2(4.5%) bacteria were Pseudomonas species. Conclusion: In the present study, it was observed that considerable numbers of Gram negative bacteria were detected from urinary tract infection cases.Bangladesh Journal of Infectious Diseases 2014;1(2):24-26

scholarly journals Identification and enumeration of microbial organisms in grey water in the Khayelitsha informal settlement, Western Cape, South Africa

2020 ◽  
Vol 20 (3) ◽  
pp. 1114-1126
Author(s):  
Qenehelo A. Leuta ◽  
James P. Odendaal ◽  
Arnelia N. Paulse
Keyword(s):  
Escherichia Coli ◽  
Informal Settlement ◽  
Most Probable Number ◽  
Bacillus Species ◽  
Western Cape ◽  
Gram Positive ◽  
Klebsiella Species ◽  
Grey Water ◽  
Probable Number ◽  
Common Misconception

Abstract Grey water, as opposed to blackwater, is generally assumed to be a safer and more acceptable wastewater source that could be considered for reuse. This is mainly due to a common misconception that its microbial load is lower compared to that of blackwater (domestic sewage). This study aimed to determine the presence of microbial contaminants, specifically pathogens, in stagnant grey water in the RR Section in Khayelitsha, Western Cape. The most probable number (MPN) technique was conducted to determine faecal coliform (FC) and Escherichia coli (EC) counts in the samples. The API 20E and the RapID ONE systems were used to identify possible pathogenic Gram-negative microorganisms, while the isolated Gram-positive microorganisms were identified using the BBL Crystal Gram-Positive (GP) Identification (ID) system. The highest respective FC and EC counts observed during this period were recorded as 2.8 × 107 microorganisms/100 mL (Site F, week 5). The RapID ONE and the API 20 identification systems identified mostly Escherichia coli, Klebsiella species and Enterobacter cloacae, amongst others, while the BBL Crystal-GP ID system identified mostly Corynebacterium and Bacillus species. The presence of these organisms raises health concerns for the community of RR Section.

scholarly journals Isolation, Screening and Methionine Production by Bacillus species isolated from Poultry Waste

2018 ◽  
pp. 95-101
Keyword(s):  
Escherichia Coli ◽  
Agar Medium ◽  
Bacillus Species ◽  
Poultry Waste ◽  
Bacillus Sp ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Solid Agar ◽  
Hemolysis Test ◽  
Oxidase Test

The current study was focused on isolating and identifying methionine producing bacteria from poultry waste. The bacteria were isolated from poultry waste and the ones which specifically produced methionine on solid agar medium, using a methionine requiring auxotroph (Escherichia coli) were kept for further analysis. Methionine assay was also carried out after fermentation to quantify methionine produced by each isolate. A total of 10 bacteria were isolated of which 2 were confirmed as active methionine producers after halo growth was observed on the solid agar medium. These 2 isolates which produced higher amount of methionine were ultimately selected for further investigation. The methionine producing isolates which were designated PW1 and PW3 were identified as Bacillus sp. respectively after biochemical tests were carried out. The test carried out include; gram staining, motility test, catalase test, citrate test, hemolysis test, indole test, penicillin sensitivity, oxidase test and crystal colony formation. Where PW3 (Bacillus sp.) produced greater amount of methionine (0.54 mg/l), while PW1 (Bacillus sp.) produced 0.20 mg/l after the completion of the methionine assay. Keywords: Keywords: Methionine production, Bacillus species, poultry waste.

The Influence of Glycosylation on the Catalytic and Fibrinolytic Properties of Pro-Urokinase

1992 ◽  
Vol 68 (05) ◽  
pp. 539-544 ◽  
Author(s):  
Catherine Lenich ◽  
Ralph Pannell ◽  
Jack Henkin ◽  
Victor Gurewich
Keyword(s):  
Escherichia Coli ◽  
Mammalian Cell ◽  
Human Gene ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Glycosylation Site ◽  
Clot Lysis ◽  
Cell Culture Media ◽  
E Coli ◽  
The Uk

SummaryWe previously found that human pro-UK expressed in Escherichia coli is more active in fibrinolysis than recombinant human pro-UK obtained from mammalian cell culture media. To determine whether this difference is related to the lack of glycosylation of the E. coli product, we compared the activity of E. coli-derived pro-UK [(-)pro-UK] with that of a glycosylated pro-UK [(+)pro-UK] and of a mutant of pro-UK missing the glycosylation site at Asn-302 [(-) (302) pro-UK]. The latter two pro-UKs were obtained by expression of the human gene in a mammalian cell. The nonglycosylated pro-UKs were activated by plasmin more efficiently (≈2-fold) and were more active in clot lysis (1.5-fold) than the (+)pro-UK. Similarly, the nonglycosylated two-chain derivatives (UKs) were more active against plasminogen and were more rapidly inactivated by plasma inhibitors than the (+)UK.These findings indicate that glycosylation at Asn-302 influences the activity of pro-UK/UK and could be the major factor responsible for the enhanced activity of E. coli-derived pro-UK.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

The size of extracellular vesicles secreted by different types of stem cells

2018 ◽  
pp. 38-42
Author(s):  
И.Б. Алчинова ◽  
М.В. Полякова ◽  
И.Н. Сабурина ◽  
М.Ю. Карганов
Keyword(s):  
Stem Cells ◽  
Extracellular Vesicles ◽  
Culture Media ◽  
Living Organism ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Study Results ◽  
Multipotent Mesenchymal Stem Cells ◽  
Rat Adipose Tissue ◽  
Almost All

Механизм терапевтического действия мультипотентных мезенхимных стволовых клеток (ММСК) на облученный организм в последнее время вызывает повышенный интерес исследователей. В качестве активного участника паракринного механизма реализации этого эффекта предлагают рассматривать внеклеточные везикулы, секретируемые практически всеми клетками живого организма. Цель работы: выделить и охарактеризовать внеклеточные везикулы, продуцируемые стволовыми клетками различной природы. Материалы и методы. Суспензии внеклеточных везикул, выделенных по модифицированному протоколу дифференциального центрифугирования из культуральных жидкостей от культур ММСК костного мозга человека 2-го пассажа и ММСК жировой ткани крысы 4-го пассажа, были проанализированы методом просвечивающей электронной микроскопии и методом анализа траекторий наночастиц. Результаты. Исследование показало наличие в обоих образцах микрочастиц размерами до и около 100 нм, однако процентное содержание частиц разных размеров в суспензии различалось для двух анализируемых типов клеток. Заключение. Полученные результаты могут свидетельствовать о специфике секреции, обусловленной клеточным типом. A mechanism of the therapeutic effect of multipotent mesenchymal stem cells (MMSC) on irradiated body has recently arisen much interest of researchers. Extracellular vesicles (EVs) secreted by almost all cells of a living organism were suggested to actively contribute to the paracrine mechanism of this effect. The aim of the study was isolation and characterization of extracellular vesicles produced by various types of stem cells. Materials and methods. Suspensions of EVs were isolated from culture media of passage 2 human bone marrow-derived MMSC and passage 4 rat adipose tissue-derived MMSC using a modified protocol of differential centrifugation and then studied using transmission electron microscopy and nanoparticle tracking analysis. Results. The study showed the presence of microparticles with a size of >100 nm in the examined samples. However, the percent content of particles with different sizes in the suspension was different in two analyzed types of cell culture. Conclusion. The study results might reflect a specificity of secretion determined by the cell type.

Sign in / Sign up

Export Citation Format

Share Document