Near-field scanning optical microscopy local luminescence studies of rhodamine dye

Open Physics ◽  
2010 ◽  
Vol 8 (3) ◽  
Author(s):  
Petr Klapetek ◽  
Juraj Bujdák ◽  
Jiří Buršík

AbstractThis article presents results of near-field scanning optical microscope measurement of local luminescence of rhodamine 3B intercalated in montmorillonite samples. We focus on how local topography affects both the excitation and luminescence signals and resulting optical artifacts. The Finite Difference in Time Domain method (FDTD) is used to model the electromagnetic field distribution of the full tip-sample geometry including far-field radiation. Even complex problems like localized luminescence can be simulated computationally using FDTD and these simulations can be used to separate the luminescence signal from topographic artifacts.

Author(s):  
E. Betzig ◽  
M. Isaacson ◽  
H. Barshatzky ◽  
K. Lin ◽  
A. Lewis

The concept of near field scanning optical microscopy was first described more than thirty years ago1 almost two decades before the validity of the technique was verified experimentally for electromagnetic radiation of 3cm wavelength.2 The extension of the method to the visible region of the spectrum took another decade since it required the development of micropositioning and aperture fabrication on a scale five orders of magnitude smaller than that used for the microwave experiments. Since initial reports on near field optical imaging8-6, there has been a growing effort by ourselves6 and other groups7 to extend the technology and develop the near field scanning optical microscope (NSOM) into a useful tool to complement conventional (i.e., far field) scanning optical microscopy (SOM), scanning electron microscopy (SEM) and scanning tunneling microscopy. In the context of this symposium on “Microscopy Without Lenses”, NSOM can be thought of as an addition to the exploding field of scanned tip microscopy although we did not originally conceive it as such.


Author(s):  
E. Betzig ◽  
A. Harootunian ◽  
M. Isaacson ◽  
A. Lewis

In general, conventional methods of optical imaging are limited in spatial resolution by either the wavelength of the radiation used or by the aberrations of the optical elements. This is true whether one uses a scanning probe or a fixed beam method. The reason for the wavelength limit of resolution is due to the far field methods of producing or detecting the radiation. If one resorts to restricting our probes to the near field optical region, then the possibility exists of obtaining spatial resolutions more than an order of magnitude smaller than the optical wavelength of the radiation used. In this paper, we will describe the principles underlying such "near field" imaging and present some preliminary results from a near field scanning optical microscope (NS0M) that uses visible radiation and is capable of resolutions comparable to an SEM. The advantage of such a technique is the possibility of completely nondestructive imaging in air at spatial resolutions of about 50nm.


Author(s):  
J. Hwang ◽  
E. Betzig ◽  
M. Edidin

Results from several different methods for probing the lateral organization of cell surface membranes indicate that these membranes are patchy, divided into domains. The data suggest that on average these domains are 0.1-1 μm across and that they persist for 10’s to 1000’s of seconds. At least some domains in this size range, when labeled by fluorescent proteins or lipids ought to be detectable by conventional, far-field, fluorescence microscopy. However, though some images are consistent with a domain structure for membranes, most far-field images of fluorescent cell surfaces lack the detail necessary to define domains.We have used near-field scanning optical microscopy, NSOM, of fluorescent-labeled cells to visualize membrane patchiness on the nanometer scale. This method yields images with resolutions of 50 nm or less. In our near-field microscope the labeled sample is illuminated by a optical fiber probe, with an aperture of 50-80nm. The probe is scanned over the cell surface at a distance of ˜ 10 nm from the surface. Only surface fluorescence is excited by the scanned probe.


2006 ◽  
Vol 129 (1) ◽  
pp. 37-43 ◽  
Author(s):  
E. X. Jin ◽  
X. Xu

Concentrating light into a nanometer domain is needed for optically based materials processing at the nanoscale. Conventional nanometer-sized apertures suffer from low light transmission, therefore poor near-field radiation. It has been suggested that ridge apertures in various shapes can provide enhanced transmission while maintaining the subwavelength optical resolution. In this work, the near-field radiation from an H-shaped ridge nanoaperture fabricated in an aluminum thin film is experimentally characterized using near-field scanning optical microscopy. With the incident light polarized along the direction across the gap in the H aperture, the H aperture is capable of providing an optical spot of about 106nm by 80nm in full-width half-maximum size, which is comparable to its gap size and substantially smaller than those obtained from the square and rectangular apertures of the same opening area. Finite different time domain simulations are used to explain the experimental results. Variations between the spot sizes obtained from a 3×3 array of H apertures are about 4–6%. The consistency and reliability of the near-field radiation from the H apertures show their potential as an efficient near-field light source for materials processing at the nanoscale.


1995 ◽  
Vol 3 (8) ◽  
pp. 3-4
Author(s):  
Sheldon Schultz

In the past few years the field of near-field scanning optical microscopy (NSOM) has developed rapidly with applications spanning all the physical sciences. A key goal of this form of microscopy is to obtain resolution at levels well beyond those possible with the usual far-field optics. In contrast to far-field optics, which is bounded by the well known limits imposed by diffraction, near-field optics has no “in principle” fundamental lower limit in lateral size, at least down to atomic dimensions, although in practice, signal-to-noise considerations may restrict the application of NSOM to a few nanometers.


Author(s):  
Eric X. Jin ◽  
Liang Wang ◽  
Xianfan Xu

Nanoscale ridge aperture antenna as a nanoscale high transmission optical device is demonstrated. High transfer efficiency and confined radiation are achieved simultaneously in the near field compared with regularly-shaped apertures. The radiation enhancement is attributed to the fundamental electromagnetic field propagating in the TE10 mode concentrated in the gap between the ridges. The transfer efficiency is further enhanced through plasmon excitation and resonance. This paper reports spectroscopic measurements of radiative transfer through bowtie shape ridge aperture antennas. Resonance in these aperture antennas and its relation with the aperture geometry are investigated. The near-field radiation through the bowtie aperture and the regular nanoaperture is also mapped with near-field scanning optical microscopy. It is revealed that plasmon excitation and resonance contribute to the radiation enhancement through the ridge aperture antennas.


1997 ◽  
Vol 474 ◽  
Author(s):  
E. B. McDaniel ◽  
J. W. P. Hsu

ABSTRACTWe incorporate a polarization modulation technique in a near-field scanning optical microscope (NSOM) for quantitative polarimetry studies at the nanometer scale. Using this technique, we map out stress-induced birefringence associated with submicron defects at the fusion boundaries of SiTiO3 bicrystals. The strain fields surrounding these defects are larger than the defect sizes and show complex spiral shapes that break the reflection symmetry of the bicrystal boundary.


Sign in / Sign up

Export Citation Format

Share Document