scholarly journals Seed priming effects on germination and first growth of the medicinal plant Achillea millefolium L.

2020 ◽  
pp. 20-23
Author(s):  
Panagiotis Kanatas ◽  
Vyronas Dellaportas ◽  
Ioanna Kakabouki ◽  
Panayiota Papastylianou

This study evaluated the effects of seed priming on germination and growth of A. millefolium by means of laboratory and greenhouse experiments conducted during 2018 in the Agricultural University of Athens. Treatments were GA3 (400 and 800 ppm), potassium nitrate (2% and 4%), polyethylene-glycol (soaking for 12 and 24h) besides an untreated control. Experiment in Petri dishes revealed that GA3 at 400 ppm, potassium nitrate (at concentration 2 and 4%) and PEG significantly increased germination percentage of A. millefolium, while germination rate was also significantly improved as a result of all seed priming techniques. In addition, due to the soil experiment, seedling emergence was significantly increased by GA3 at 400 ppm, potassium nitrate (at both concentrations) and PEG compared with the untreated seeds. Dry biomass of the young seedlings was significantly enhanced by means of GA3 (at 400 and 800 ppm), KNO3 (4%) and PEG for 24 h, indicating the potential effect of seed priming on first growth as well. The results of the present study revealed the significant positive effects of seed priming on A. millefolium seed germination, seedling emergence and early growth.

2011 ◽  
Vol 3 (4) ◽  
pp. 113-116 ◽  
Author(s):  
Hamdollah ESKANDARI ◽  
Kamyar KAZEMI

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.


Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.


Botany ◽  
2018 ◽  
Vol 96 (5) ◽  
pp. 319-328 ◽  
Author(s):  
Jeremi Kołodziejek ◽  
Jacek Patykowski ◽  
Mateusz Wala

The germination requirements of four taxonomically related taxa of Dianthus (D. arenarius L. subsp. borussicus Vierh., D. carthusianorum L., D. gratianopolitanus Vill., and D. deltoides L.) were studied under controlled conditions in a laboratory. A set of experiments were conducted to evaluate seed germination responses to storage period and cold stratification, different hydrogen ion concentrations (pH), potassium nitrate ([Formula: see text]), temperatures, and light conditions. Experiments were also performed to study the effects of sowing depth and water supply on seedling emergence. There were no differences in germination between seeds incubated under light or dark conditions for any of the taxa we studied. Germination percentages increased significantly with increases in temperature. The taxa of Dianthus we studied showed similar responses to sand burial, i.e., seeds placed on or near the soil surface had maximum emergence, and emergence declined with increasing depth of seed burial. In the studied taxa, germination was reduced by declining osmotic potentials. Cold stratification increased the germination rate, but did not affect final germination percentage of Dianthus seeds. A higher concentration of nutritious solutions (KNO3) negatively affected the germination percentage. The results show that seeds of the studied taxa are potentially germinable in the species’ habitat at any time between April and October.


HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 912D-912
Author(s):  
Ahmet Korkmaz ◽  
Wallace Pill

Achievement of head size uniformity at final harvest reduces loss and increases profitability for the hydroponic lettuce grower. Shoot fresh weight of `Cortina' lettuce (Lactuca sativa L.) at 7 or 21 days after planting (DAP) was inversely proportional to the number of days required for seedling emergence, and was greater for raw than for pelleted seeds. Head fresh weight at final harvest (61 DAP) was directly proportional to seedling length at 21 DAP, but raw and pelleted seeds produced equal head weights. Thus, initial seed (seedling) vigor differences were maintained to final harvest. Osmotic seed priming (–1.5 MPa KH2PO4, 20 h, dark) led to increased germination rate at 15, 25, and 35C; had no effect on germination synchrony; and increased germination percentage only at 35C. Covering raw or pelleted seeds sown in depressions of the phenolic foam trays with fine (No. 5) vermiculite compared to leaving the seeds uncovered, and soaking the trays in hydroponic solution rather than water, increased seedling shoot fresh weights. Seeds sown on their first day of germination or primed seeds gave greater seedling shoot fresh weights than pelleted seeds. However, the more uniform seedling shoot fresh weights from germinated seeds than from primed seeds was associated with more rapid and synchronous seedling emergence.


Author(s):  
Hadi Pirasteh-Anosheh ◽  
Seyedeh-Elahe Hashemi

Salinity as a major constrain of plant productivity affects growth and development at any stage of growth cycle. Under saline conditions, rapid and uniform germination of plants would be necessary to achieve enhanced quality and potential yield. In such situation, seed priming could be an important approach to increase plant germination, growth and consequently yield. Priming is defined as seed presowing in solutions that allow them to imbibe water to improve the first stage of germination; however, this prevents radical protrusion through seed coat. Seed priming improves establishment in many plant species, and include hydro-priming, osmo-priming, halo-priming, thermo-priming and hormo-priming. Overall, accelerated biochemical and physiological process such cell division and starch hydrolysis, induced α-amylase activity, greater germination rate, less Na+ and higher K+ accumulation and induced antioxidative system might be some mechanisms for salinity tolerance in primed plants. Since shortage of water availability due to osmotic stress is the first phase in salt stress, so one of the major mechanisms for improved germination and growth in primed seeds is accelerated water imbibition under saline conditions. In this paper, different seed priming strategies are examined and comprised in different plants growing in saline conditions. Since different types of seed priming have positive effects on germination, emergence, growth, yield as well as biochemical traits and quality of plants, it seems that seed priming could be promising approach for improved salinity tolerance in future world with changed climate. More researches on detail of seed priming for each agent is needed for each plant.


2015 ◽  
Vol 43 (1) ◽  
pp. 153-158 ◽  
Author(s):  
Faruk TOKLU

An experiment was conducted under laboratory and field conditions in order to evaluate the effects of different priming treatments, specifically KNO3 (1%), KCl (2%), KH2PO4 (1%), ZnSO4 (0.05%), PEG-6000 (20%), IBA (100 ppm), Mannitol (4%), GA3 (100 ppm) and distilled water, on seed germination properties and several agro-morphological plant characteristics of red lentil. Seeds not primed were used as a control. GA3 treatment increased shoot length. The control (non-primed seeds) treatment resulted in increased seedling root number and length. Distilled water, ZnSO4 and control treatments increased germination rate and percentage. In the pot experiments, GA3 treatment increased plant height and seedling emergence rate, whereas KCl treatment improved the number of nodules, as well as root and shoot dry weight when compared to the control. ZnSO4 treatment increased yield components and grain yield in field conditions. The results of this study showed that ZnSO4, GA3 and PEG-6000 seed priming treatments may be useful tools due to their positive effects on germination rate, germination percentage, yield component and grain yield of lentil.


PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.


2018 ◽  
Vol 36 ◽  
Author(s):  
M. REZVANI ◽  
S.A. SADATIAN ◽  
H. NIKKHAHKOUCHAKSARAEI

ABSTRACT: Our knowledge about seed dormancy breaking and environmental factors affecting seed germination of greater bur-parsley (Turgenia latifolia) is restricted. This study has addressed some seed dormancy breaking techniques, including different concentrations of gibberellic acid (GA3) and potassium nitrate (KNO3), leaching duration, physical scarification as well as some environmental factors effective on seed germination such as salt and drought stresses, pH and seed planting depth. Seed germination was promoted with lower concentrations of KNO3 (0.01 to 0.02 g L-1), while higher concentrations reduced germination percentage. Seed dormancy was declined by low concentrations of GA3 up to 100 ppm. Seeds of greater bur-parsley germinated in a range of pH from 3 to 7. With enhancement of drought and salt stresses, seed germination decreased. Also, there was no seed germination in a high level of stresses. Seedling emergence reduced as planting depth increased. Use of GA3, KNO3, leaching and physical scarification had a positive effect on seed dormancy breaking of greater bur-parsley. The information from the study increases our knowledge about seed dormancy breaking techniques, response of germination to drought and salt stresses and also determination of distribution regions of greater bur-parsley in the future.


HortScience ◽  
1994 ◽  
Vol 29 (8) ◽  
pp. 868-870 ◽  
Author(s):  
Amy M. Fay ◽  
Mark A. Bennett ◽  
Steven M. Still

Low-vigor seeds of black-eyed Susan (Rudbeckia fulgida Ait.) primed in aerated -1.3 MPa KNO3 for 7 days at 30C in darkness had double the total germination percentage at 30C and one-half the mean time of germination as nonprimed seeds. Priming the seeds in polyethylene glycol rather than KNO3 generally resulted in lower total germination percentage and longer mean time of germination. Osmotic priming increased total germination percentage and germination rate of seeds germinated at 21.9 to 32.2C, but the priming benefit on total germination percentage was greater at ≤27.6C. Total germination percentage of primed and nonprimed seeds was highest at 28.8 to 32.2C.


HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553b-553
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum

Various osmotic solutions and solid carriers have been used to prime (controlled hydration followed by drying) seeds. Broccoli (Brassica oleracea L., var. italica, cvs. Brigadier and Earlidawn) seeds were primed in solutions of polyethylene glycol, potassium nitrate (KNO3), and mannitol or calcium silicate (Micro-ccl E) and vermiculite to determine which treatment provided the greatest enhancement of germination. Germination percentage and rate as well as head yield were determined in a series of laboratory, glasshouse, and field experiments over a three year period. Water potentials (Y) ranged from -0.8 to -2.6 MPa at 20°C for both osmotic solutions and carriers. The duration of the priming treatments were 3, 7, or 10 days. All priming treatments, except KNO3 and mannitol, consistently reduced the mean time to germination in the laboratory, reduced the mean time to field emergence, increased final stands, but did not affect the mean time to harvest. However, solid phase was more effective than osmotic priming at each Ψ tested. The best solid priming mixture was 1.0:0.8:1.8 (seed:carrier:water) for 7 days at 20°C using Micro-cel E. This treatment was measured to have a Y = -1.2 MPa, and other types of priming at the same Y were less effective. The Y threshold below which no priming effect was obtained was Ψ= -2.4 MPa. Priming response was closely associated with, but not entirely dependent on, the Ψ of the treatment.


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