Wheat is one of the most important cereals grown in the world, and in Brazil, increasing national production is still a challenge. Nitrogen (N) supply can favor grain yield and the physiological quality of wheat seeds. However, the definition of adequate N rate and fertilization timing must consider genotype, cultivation environment, and initial seed vigor level. The aim of this work was to evaluate the effect of initial seed vigor and the combinations of rates and timings of N application on grain yield and seed physiological quality of wheat cultivars under different edaphoclimatic conditions. The experiment was carried out in Londrina and Ponta Grossa, state of Paraná, in a randomized block design in a 2 × 2 × 7 factorial scheme, with four replicates. Treatments consisted of two seed vigor levels (vigorous and non-vigorous seeds), two wheat cultivars (BRS Gralha-Azul and BRS Sabiá) and seven combinations of fertilization timings and N rates (kg ha-1) (control-0N; 20N at sowing and 60N at tillering; 40N at sowing; 80N at sowing; 40N at sowing and 40N at tillering; 40N at tillering; 80N at tillering). Number of emerged seedlings, vegetation index, shoot dry matter, number of fertile spikes m-2 and grain yield were evaluated. Additionally, the physiological potential of seeds produced in Londrina was evaluated by the testes of first count, germination, seedling emergence in sand and emergence speed index. The climatic conditions during the experiment, in both cultivation environments, were similar to average historical records, with some periods of water deficit. The highest grain yield was obtained with the BRS Gralha-Azul in Londrina, and with BRS Sabiá in Ponta Grossa. The use of vigorous seeds favored the stand establishment and the response of plants to N fertilization. The treatments 40N + 40N, and 40N + 0N favored the majority of evaluated variables. Both cultivars showed potential for the production of high physiological potential seeds in Londrina environment. Inadequate N supply impairs wheat grain yield and seed production.