scholarly journals Uji Aktivitas Antibakteri Propionibacterium acnes Ekstrak Etanol dan Fraksi Daun Karuk (Piper sarmetosum Roxb. Ex. Hunter) serta Analisis KLT Bioautografi

1970 ◽  
Vol 1 (1) ◽  
pp. 9-15
Author(s):  
Anisa Dwi Nuraeni ◽  
Yani Lukmayani ◽  
Reza Abdul Kodir
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Water Fraction ◽  
Liquid Liquid Extraction ◽  
Tlc Bioautography ◽  
Ethanol Extracts

Abstract. Karuk (Piper sarmetosum Roxb. Ex. Hunter) is a plant that is considered to have antibacterial activity. Acne is a problem in the skin caused by the bacteria Propionibacterium acnes. This research’s aimed to determine the potential antibacterial activity of ethanol extracts and karuk leaf’s fractions, and can find out the class of chemical compounds contained from the ethanol extract and the fraction of karuk leaves which have antibacterial activity. Karuk leaf was extracted by maceration method using 96% ethanol solvent, followed by fractionation using the liquid-liquid extraction method with n-hexane, ethyl acetate, and water solvents. Antibacterial activity test of ethanol extract and fraction with a concentration of 2%, 4%, 6%, and 8% using the well diffusion agar method. To identify the class of compounds that have antibacterial activity, the TLC Bioautography method is used. From the results of research that has been done shows that ethanol extract and n-hexane fraction can inhibit the growth of Propionibacterium acnes with the highest inhibitory diameter of 10.11mm in the extract, and in the n-hexane fraction of 10.93 mm. in the ethyl acetate fraction and the water fraction there is no inhibitory zone formed so that it cannot provide antibacterial activity. The TLC Bioautography results did not show any inhibitory zones and there was no change in color when given a spotting viewer. So that compounds cannot be identified that have antibacterial activity Abstrak. Karuk (Piper sarmetosum  Roxb. Ex. Hunter) merupakan tanaman yang diketahui memiliki aktivitas sebagai antibakteri. Jerawat merupakan permasalahan pada kulit disebabkan adanya bakteri Propionibacterium acnes. Penelitian ini bertujuan untuk menentukan potensi aktivitas antibakteri dari ekstrak etanol dan fraksi daun karuk, serta dapat mengetahui golongan senyawa kimia yang terkandung dari hasil ekstrak etanol dan fraksi daun karuk yang memiliki aktivitas antibakteri. Daun karuk diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%, dilanjutkan dengan fraksinasi menggunakan metode ekstraksi cair-cair dengan pelarut n-heksan, etil asetat, dan air. Uji aktivitas antibakteri ekstrak etanol dan fraksi dengan konsentrasi 2%, 4%, 6%, dan 8% menggunakan metode difusi sumuran agar. Untuk mengetahui golongan senyawa yang memiliki aktivitas sebagai antibakteri digunakan metode KLT Bioautografi. Dari hasil penelitian yang telah dilakukan menunjukan bahwa ekstrak etanol dan fraksi n-heksan dapat menghambat pertumbuhan Propionibacterium acnes dengan diameter hambat tertinggi 10,11mm pada ekstrak, dan pada fraksi n-heksan 10,93 mm.  pada fraksi etil asetat dan fraksi air tidak terbentuk adanya zona hambat sehingga tidak dapat memberikan aktivitas sebagai antibakteri. Hasil KLT Bioautografi tidak menunjukan adanya zona hambat serta tidak terdapat perubahan warna ketika diberikan penampak bercak. Sehingga tidak dapat teridentifikasi senyawa yang memiliki aktivitas antibakteri.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

scholarly journals Antibacterial Activity of Extract and Fraction From Shitake Mushroom (Lentinula edodes) Against Acne Bacteria

2018 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Ika Kurnia Sukmawati ◽  
Ari Yuniarto ◽  
Widhya Alighita ◽  
Ade Zam-zam J
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Lentinus Edodes ◽  
Shiitake Mushrooms ◽  
And Staphylococcus Aureus

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

2019 ◽  
pp. 8-14
Author(s):  
Risa Supringrum ◽  
Siti Jubaidah
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Hexane Fraction ◽  
High Dose ◽  
Water Fraction ◽  
Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

scholarly journals Antiproliferation activity of God's crown fruit (Phaleria macrocarpa) extract and fractions against MCM-B2 breast cancer cells

2020 ◽  
Author(s):  
Ratna Puspita ◽  
Hasim ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Water Fraction ◽  
Activity Test ◽  
Phaleria Macrocarpa

Breast cancer is a disease caused by abnormal cell proliferation in the breast. God’s crown fruit (Phaleria macrocarpa) and its seed have potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids, alkaloids, polyphenols, and tannins. The sample of God’s crown fruit was obtained by extraction and fractionation using the maceration method. Cytotoxicity of extracts and fractions was determined using the Brine Shrimp Lethality Test method. The antiproliferation activity test of God’s crown fruit against MCM-B2 was performed using the hemacytometer method. The God’s crown fruit sample consists of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. Lethal concentration 50 (LC50) values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72, 147.55, 405.81, and 149.55 ppm, respectively. The concentration of the test sample was directly used for the antiproliferation activity test on MCM-B2 cells. God’s crown fruit can act as an antiproliferation of MCM-B2. The smallest concentration of those samples has inhibited MCM-B2 cell proliferation which is 3.5 ppm crude ethanol extract lower than 100 ppm doxorubicin. The maximum percentage of the antiproliferation activity of crude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm) by 31.2%. This is due to the fact that crude ethanol extract has a lot of complex polar phytochemical content. The crude ethanol extract compounds inhibit MCM-B2 cell proliferation synergistically.

scholarly journals ANTIBACTERIAL ACTIVITY OFMALACCA FRUIT (PHYLLANTUS EMBLICA L.)ETHANOLIC EXTRACT AND FRACTIONAGAINSTBACILLUS CEREUS FNCC0057 ANDSHIGELLA DYSENTERIAE ATCC13313

2017 ◽  
Vol 10 (14) ◽  
pp. 8
Author(s):  
Tiana Milanda ◽  
Arif Satria Wira Kusuma ◽  
Kuganesh Shanmuganathan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Water Fraction ◽  
Dried Fruit

Diarrhea is a symptom of a disease, which is characterized by increased frequency of defecation (more than three times a day) with a more fluid of feces. In Ayurvedic medicine, the fruit of Malacca (Phyllanthus emblica L.) is often used as an antibacterial and antiviral against various infectious diseases. This study aims to determine:(i) the antibacterial  activities of ethanol extract and its fraction of Malacca fruit, and (ii) determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against diarrhea-causing bacteria, such as Bacillus cereus FNCC0057 and Shigella dysenteriae ATCC13313. The study was  conducted through several processes: (i) the determination of the plant and  preparation dried fruit, (ii) extraction of dried fruit, fractionated extract, antibacterial activity test of ethanol extract and its fractions, (iii) determination MIC and MBC from the most active fractions and (iv) phytochemical screening of fraction. The results showed that ethanol extract, water fraction and ethyl acetate  fraction of Malacca fruit has antibacterial activities against B. cereus FNCC0057 and S. dysenteriae ATCC13313, where the greatest activity was shown by the fraction of ethyl acetate. This fraction has MIC and MBC values of 0.187 - 0.375% (w/v) and and 0.09375 - 0.1875% (w/v) against B. cereus FNCC0057 and S. dysenteriae ATCC13313. The antibacterial activity of this fraction was probably derived from alkaloid, flavonoid, tannin, quinone, saponin, monoterpene or sesquiterpene compounds. 

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