scholarly journals Pengaruh Sitokinin Eksogen dan Sukrosa terhadap Produksi Biomassa dan Alkaloid Canthinone di dalam Kultur Suspensi Sel Pasak Bumi (Eurycoma longifolia Jack.)

2012 ◽  
Vol 12 (2) ◽  
pp. 143
Author(s):  
Luthfi Aziz Mahmud Siregar
Keyword(s):  
Cell Suspension ◽  
Sucrose Concentration ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Eurycoma Longifolia ◽  
Eurycoma Longifolia Jack

The effect of addition cytokinins and modification of sucrose concentration on growth and alkaloid canthinoneproduction in cell suspension cultures of Eurycoma longifilia Jack were studied. The additions of cytokines, BAand kinetin, show effect on the production of biomass and alkaloid in cell suspension of E. longifilia Jack. Theoptimum totals of two-alkaloids were obtained on addition 4.44 μM BAP and without kinetin, respectively. Theaddition of 4.44 μM BA (6-benzyladenine) into TAM medium stimulated increased total of 9-hydroxycanthine-6-one,but decreased total of 9-methoxycanthin-6-one. While the addition of 2.32 - 9.29 μM kinetin (6-furfurylaminopurine)into TAM medium decreased total of two alkaloids (from 0.582 mg to 0.461 - 0.257 mg per 25 ml medium). Whensucrose concentration in TAM medium was increased from 3% to 5%, production of biomass would increase from0.374 g to 0.585 g dry weight per 25 ml medium. While total of two-alkaloids increase from 0.328 mg to 0.441 mgper 25 ml medium when concentration of sucrose in TAM medium was increased from 3% to 4% sucrose.

A Comparison of Various Growth Parameters of Cell Suspension Cultures to Determine Phytotoxicity of Xenobiotics

Weed Science ◽  
1984 ◽  
Vol 32 (2) ◽  
pp. 235-242 ◽  
Author(s):  
David G. Davis ◽  
Rosa L. Stolzenberg ◽  
Joan A. Dusky
Keyword(s):  
Ethylene Oxide ◽  
Cell Suspension ◽  
Culture Medium ◽  
Growth Parameters ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Weight Changes ◽  
Advantages And Disadvantages ◽  
Cell Volumes

An assessment was made of various parameters to measure growth of soybean [Glycine max (L.) Merr. ‘Wilkin’] and einkorn (Triticum monococcum L.) cell suspension cultures to establish convenient methods of screening the effects of chemicals. Methods assessed were settled cell volumes, packed cell volumes, absorbance at 525 nm of sonicated aliquots, dry weights (of aliquots or entire flask contents), and electrical conductivity and pH of the culture medium. Settled cell volumes, conductivity, and dry-weight changes were the most useful of the methods tested for determining the phytotoxicity of a nonionic linear alcohol ethylene oxide detergent (an adduct of 1-dodecanol containing eight ethylene oxide units) and the methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid}. Because 3 to 4 weeks were required to assess whether the cultures could grow out of the initial inhibition by the detergent or herbicide, none of the methods was rapid. Advantages and disadvantages of the various methods and their relative values for screening compounds are described.

scholarly journals Dicentrine Production in Callus and Cell Suspension Cultures of Stephania Venosa

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tharita Kitisripanya ◽  
Jukrapun Komaikul ◽  
Nirachara Tawinkan ◽  
Chuennapha Atsawinkowit ◽  
Waraporn Putalun
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Alternative Source ◽  
Plant Materials ◽  
Stem Segments

The highest dicentrine content (19.5 ± 0.3 mg/g dry weight) from callus culture of Stephania venosa was achieved from stem segments cultured on MS medium supplemented with TDZ 0.5 mg/L and NAA 1.0 mg/L. Cell suspension cultures were established from callus cultured on MS liquid medium with the same plant growth regulators. Dicentrine production from S. venosa cell suspension cultures was obtained in the range of 15–26 mg/g dry weight. Elicitation in cell suspension cultures by chitosan (50 mg/L) and salicylic acid (2 mg/L) for 6 days significantly increased dicentrine content. Our findings indicate that callus and cell suspension cultures of S. venosa can produce high levels of dicentrine as an alternative source of plant materials.

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Fuyuan Jing ◽  
Ling Zhang ◽  
Meiya Li ◽  
Yueli Tang ◽  
Yuliang Wang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Cell Suspension ◽  
Biosynthetic Pathway ◽  
Gene Expression Analysis ◽  
Artemisia Annua ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Expression Of Genes ◽  
Artemisinin Content

AbstractArtemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 µM) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 µM ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.

scholarly journals Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

2001 ◽  
Vol 36 (9) ◽  
pp. 1085-1092 ◽  
Author(s):  
Marcia Ometto de Mello ◽  
Antônio Francisco de Campos Amaral ◽  
Murilo Melo
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Sucrose Synthase ◽  
Sucrose Concentration ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Curcuma Zedoaria ◽  
Metabolizing Enzymes ◽  
Sucrose Metabolizing Enzymes ◽  
Bauhinia Forficata

The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.

Callus and cell suspension culture of bush bean (Phaseolus vulgaris)

1970 ◽  
Vol 48 (6) ◽  
pp. 1119-1130 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Cell Number ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Coconut Milk ◽  
Mineral Salts ◽  
Bush Bean

A solid medium was developed for callus cultures originating from explants of root, hypocotyl, and cotyledon of seedlings of bush bean, and a liquid medium was developed for the growth of cell suspension cultures derived from the callus cultures. Some unsatisfactory media are recorded. Concentrations of mineral salts for cell suspension cultures are lower than for callus cultures. Both coconut milk and other organic substances are required for maximum growth. With cell suspensions the effect of deproteinized coconut milk is the same as that of raw coconut milk but, with callus cultures, deproteinized coconut milk gives greater yield. There are no obvious differences in yield of callus derived from root, hypocotyl, or cotyledon. Few differences in yield were obtained between cell suspension cultures from root, hypocotyl, and cotyledon but those from root gave the highest yield in dry weight. However, in the same medium, cells from the three origins are very similar in form and appearance. Some effects of different media on cell form and clumping are described. The yield in suspension culture is very high. Increase in cell number, fresh weight, and dry weight is about 100-fold in 12 days involving about six to seven divisions per cell.

Enhanced anthraquinone production in presence of silver nitrate in suspension culture of Gynochthodes umbellata (L.) Razafim. & B. Bremer (Rubiaceae), a traditional medicinal plant mentioned in Hortus Malabaricus

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Gangaprasad A
Keyword(s):  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Ms Medium ◽  
Fresh Weight ◽  
Friable Callus

Silver nitrate (AgNO3) enhanced production of anthraquinone was standardized in cell suspension cultures of Gynochthodes umbellata, a plant mentioned in the Hortus Malabaricus. The present research investigates the effect of silver nitrate, an abiotic elicitor on production of anthraquinone in in vitro cell suspension cultures of G. umbellata. Friable callus culture was established using in vitro derived leaf segment obtained from the nodal explant culture maintained in Murashige and Skoog (MS) medium containing 2 mg/l benzyl amino purine (BAP) and 3% sucrose. The in vitro derived leaf segments (0.5cm2) were cultured on MS medium containing 1 mg/l 2,4-D and 2% glucose for the production of friable callus. After 30 days of culture, uniform yellow friable callus was inoculated into MS liquid medium containing 1 mg/l 2,4-D and 2 % glucose for raising suspension culture. Uniform cell suspension was transferred to same media constituents and treated with different concentrations of AgNO3 on 25th day of culture. Fresh weight, dry weight and accumulation of anthraquinone content was studied and found that AgNO3 caused a marginal increase in biomass and anthraquinone based on the concentration and duration of AgNO3 treatment. A maximum fresh weight (19.48 g/fwt) dry weight (1.92g/dwt) and highest amount of anthraquinone content (48.62 mg/gdwt) were recorded in MS medium supplemented with 1 mg/l 2,4-D, 2%glucose and 3.5µM AgNO3 after 72 hrs of incubation.

scholarly journals Production of Honokiol and Magnolol in Suspension Cultures of Magnolia Dealbata Zucc

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Fabiola Domínguez ◽  
Marco Chávez ◽  
María Luisa Garduño-Ramírez ◽  
Víctor M. Chávez-ávila ◽  
Martín Mata ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Dry Weight ◽  
Growth Cycle ◽  
Suspension Cultures ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Magnolia Dealbata

Honokiol and magnolol, important anxiolytic and anti-cancer agents, have been produced in cell-suspension cultures of the endangered Mexican plant Magnolia dealbata Zucc. In vitro cultures of the plant were established, and the accumulation of honokiol and magnolol in callus and cell-suspension cultures was measured. Leaf samples were the best explants for callus establishment and metabolite production, and Murashige and Skoog medium supplemented with 1 mg/L 2, 4-dichlorophenoxyacetic acid and 1 mg/L kinetin yielded 2.3 mg/g of honokiol and 5.9 mg/g of magnolol. Bacterial and fungal contamination was inhibited with a multiple-step tissue sterilization procedure. Oxidation was inhibited with 1 g/L activated charcoal. Cell-suspension batch cultures derived from friable callus obtained from leaves of this species were grown for 30 days in shaker flasks containing Murashige and Skoog medium. Throughout the growth cycle, honokiol and magnolol levels, fresh and dry weight, and sucrose uptake were determined. The effects of carbon source concentration on biomass accumulation and the synthesis of bioactive compounds were studied. By using 3 mL of inocula supplemented with 3% (w/v) sucrose, maximum yields of honokiol (8.1 mg/g) and magnolol (13.4 mg/g) were obtained after 25 days. These yields were 300% and 382%, respectively, of the yields of honokiol and magnolol obtained from field-grown plants.

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