scholarly journals The Effect of Turmeric Extract (Curcuma zedoria) on Antioxidant Enzymes Activity

2020 ◽  
Vol 5 (1) ◽  
pp. 31
Author(s):  
Chris Adhiyanto ◽  
Lucky Briliantina ◽  
Narila Mutia Nasir ◽  
Hari Hendarto ◽  
Flori R Sari ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Free Radicals ◽  
Monosodium Glutamate ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Turmeric Extract ◽  
Endogenous Antioxidants ◽  
Endogenous Antioxidant

Background: Indonesia is a country that has diversity of spices and types of cuisine. In the early 70s, the use of MSG (monosodium glutamate) as a flavor enhancer began to be popular in Indonesia. It replaces natural flavor such as sugar, salt or spices because its affordable price compared to natural flavor. MSG as food additive is added to foods that exceed limits and may cause a negative effect to the body. One of the effects is the increasing of free radicals that can lead to various degenerative diseases such as blood vessel disorders and others. White turmeric is a common spice that widely used by Indonesian. Several studies have reported the benefits of white turmeric as an antifungal, antimicrobial and so on. The objective of this research is to find the effects of white turmeric extract in improving endogenous antioxidants to suppress the free radical effects because of the excessive use of MSG.Materials and Method: We measured the activity of SOD, catalase and GSHreductase as endogenous antioxidants in mice treated with MSG and white turmeric. The subjects of this study used 24 Sprague Dawley male rats aged 2-6 months; weight 100-150 grams, which were randomly divided into 6 groups. The control group did not receive any treatment. The MSG group was given MSG (4800mg / kg/ day). Groups 3 and 4 were given MSG and white turmeric extract, respectively (100 mg / kg / day and 200 mg / kg / day). Groups 5 and 6 were given MSG and vitamin C respectively (250 mg / kg / day and 500 mg / kg/ day) after 14 days of treatment; the activity of enzyme measured by Abcam Enzyme Kit and detected by Spectrophotometer Thermo Scientific Multi-scan Go.Result: The administration of white turmeric extracts will increase SOD and Catalase activity, and reduce GSH-reductase activity.Conclusion: White turmeric extract will help the work of endogenous antioxidant enzymes in counteracting free radicals produced from MSG metabolism.International Journal of Human and Health Sciences Vol. 05 No. 01 January’21 Page: 31-34

THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 39
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p>Key words: MSG, curcuma, endometrium</p>

scholarly journals Mangosten Skin Extracts Decrease The Activity of The Gamma-Glutamyltransferase (α-GT) Serum On The Cigarette Exposure

2017 ◽  
Vol 3 (1) ◽  
pp. 29
Author(s):  
Loo Hariyanto Raharjo ◽  
Hwa Tjeen Anna Lewi Santoso
Keyword(s):  
Free Radicals ◽  
Cigarette Smoke ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Treatment Groups ◽  
Post Test ◽  
Increased Activity

Exposure to cigarette smoke can cause oxidative stress, which characterized by increased activity of g-GT serum. The skins of the mangosteen (Garcinia mangostana L.) contain anthocyanins pigments, which can act as antioxidants to reduce free radicals. This study mainly aims to investigate the effect of mangosteen skin extracts to decreased activity of g-GT serum in rats exposed to cigarette smoke. This study was true experimental designs with randomized post-test only control group design. This study used thirty male rats were dividing into six treatment groups. Rats exposed to cigarette smoke, which came from two cigarettes, for 4 weeks lead to an increase free radicals in the body. The increased activity of g-GT serum to damp the free radicals. Mangosteen skin extracts can reduce free radicals so that the activity of g-GT serum decreased.The conclusion of this research is the mangosteen skin extracts can decrease the activity of the g-GT serum.

scholarly journals THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 38
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p><strong>Keywords</strong>: MSG, curcuma, endometrium</p>

scholarly journals Histological and Antioxidant Effect of different phone waves on the liver and kidney of male rats

2019 ◽  
Vol 10 (4) ◽  
pp. 3554-3559
Author(s):  
Nuha Sh. Ali ◽  
Haidar Alsaedi
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Cell Phone ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Ranges ◽  
Total Antioxidant ◽  
Liver And Kidney

There is tremendous concern about the potential harmful effects of cell phone microwaves. The results are contradictory. However, the effects of these waves on the body have been reported. In this study, the effect of cellular microwaves on sperm parameters and total antioxidant capacity in relation to the duration of exposure and frequency of these waves were studied. This experimental study was conducted on male Sprague Dawley rats (150-200 g). The animals were randomly assigned to three groups (n = 18). Group 1 Natural control rat were fed to standard laboratory feeding Animals for 14 days. Group 2 animals were exposed to cell phone receiver stimulated waves (915 MHz frequency) for 14 days, and Group 3 animals were exposed to simulated waves of a cell phone antenna (950 MHz frequency) for 14 days. The results indicated that the total antioxidant capacity in all exposure groups decreased significantly compared with the control group (P <0.05). Increased exposure time within two weeks caused a statistically significant decrease in sperm motility and mobility (P <0.05).Effect of phone waves on liver and kidney of male Sprague Dawley rat. The study of pathological anatomy revealed a clear and appropriate deviation. These results found blood enzymes such as amino-aspartase transferase (AST), amino amines (ALT), and creatinine levels. The results showed no significant changes in both liver and kidney physiological functions. Malondialdehyde level (MDA) and total antioxidant contents were performed. The level of glutathione (GST), superoxide dismutase (SOD) was in normal ranges. Exposure to cell phone waves can reduce sperm movement and mobility in rats. These waves can also reduce the total antioxidant capacity in rats and lead to oxidative stress.

scholarly journals Hypoglicemic and Antioxidant Activities in Extracts Star Anise (Illcium Verum Hook.f) on Wistar Rat (Rattus norvegicus) Diabetes

2020 ◽  
Vol 4 (1) ◽  
pp. 34-43
Author(s):  
Anna Maria Dewajanthi ◽  
Agus Limanto ◽  
Clarita Clarita ◽  
Audrey Fidelia
Keyword(s):  
Diabetes Mellitus ◽  
Free Radicals ◽  
Blood Sugar ◽  
Positive Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group ◽  
Star Anise ◽  
Endogenous Antioxidants ◽  
Sugar Levels

Diabetes mellitus is a disease that is quite common among the people. The causes of diabetes mellitus vary depending on the classification of this group of diseases. If diabetes mellitus is not handLed properly and correctly, there will be chronic hyperglycemia that causes oxidative stress, so patients will complain of some complications from diabetes mellitus that is actually caused by free radicals. Star anise (Illicium Verum) is one of the plants that are often used, one of which is as a spice in food. The star anise  has several compounds, one of which is flavonoid which is as an easily oxidized compound (antioxidant), so it is often used as a traditional medicine to treat many diseases. In several studies in vitro this star anise plant has also been proven to have antioxidant levels such as flavonoids and phenolic which are high enough so that it can be used as a source of exogenous antioxidants that can help endogenous antioxidants such as glutathione (GSH) to reduce free radicals that enter and remain keep body cells in normal condition. The study was carried out experimentally and conducted on rats in diabetes mellitus to see the effect of giving star anise extracts on glucose levels and blood levels of endogenous antioxidants (glutathione). Male rats with 2-3 months of age as many as 24 animals were divided into 6 groups, namely the negative control group (mice only given distilled water), diabetic rats without treatment group (positive control), diabetic rats treated with rootbose (positive control), and diabetic rats by treating the extract of star anise of 50, 100, and 150 mg/kg body weight of rats. This research was conducted for 7 days. GSH levels were measured on days 0 and 7. To analyze the differences in the average data of glucose and GSH, statistics were used. Results of the Wilcoxon test showed that there were significant differences (with p < 0.05) in blood sugar levels between the control group and the diabetic rats treated with star anise extract group. There was a significant decrease in blood sugar levels in diabetic rats given an extract of star anise. The greatest decrease in blood sugar levels occurred in the group of diabetic rats who were given an extract of star anise at 150 mg/kg bw. It is also known that there are difference in GSH levels between the control group and the diabetic rat group with the treatment of extract of star anise, but statistically the difference is not significant. GSH levels increased 0.03 µM/mL by administering an extract of star anise at 50 mg/kg bw.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

scholarly journals Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

2020 ◽  
Vol 19 (2) ◽  
pp. 296-303
Author(s):  
Eni Widayati ◽  
Taufiqurrachman Nasihun ◽  
Azizah Hikma Savitri ◽  
Nurina Tyagita
Keyword(s):  
Protein Expression ◽  
Caspase 3 ◽  
Medical Science ◽  
Liver Cells ◽  
Male Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Uvb Irradiation ◽  
The Difference

Objective: The effect of Pimpinela alpina Molk (PaM) on decrease in Bax and Caspase-3 protein expression in liver cells apoptosis have been proven. However, the difference result between 7 and 15 days treatment duration of PaM need to be confirmed. This study aimed to confirm that treatment of PaM during 15 days is more effective decreasing Bax and Caspase-3 protein expression in liver cells following UVB irradiation. Methods: In the post test only control group design, 35 Sprague Dawley male rats, 300 gram body weight were divided into two arms, consisting of three groups respectively. First arm comprise Neg-7, PaM7-100, and PaM7-150. Second arm comprise Neg-15, PaM15-100, and PaM15-150. Nor-G was added as normal control neither exposed to UVB nor PaM treatment. In negative group was only radiated to UVB and PaM groups were exposed to UVB and treatment with 100, and 150 mg PaM per oral for 7 and 15 days respectively. At day 8 (first arm) and 16 (second arm), liver organ was taken and Bax and Caspase-3 protein expression assessed by Immunohistochemical staining method. Result: Post Hoc LSD analysis indicated that Bax and Caspase-3 protein expression in PaM15-100 and PaM15-150 was significant lower compared to that of Nor-G, PaM7-100, and PaM7-150, p < 0.05. Conclusion: Ttreatment of PaM with doses 100 and 150 mg for 15 days was better in decreasing Bax and Caspase-3 protein expression of liver cells following UVB irradiation. Bangladesh Journal of Medical Science Vol.19(2) 2020 p.296-303

scholarly journals Peculiarities of Evaluation of Biological Effects of the Influence of Electromagnetic Radiation in Conditions of Reduced Temperature (Experiment)

2021 ◽  
Vol 6 (2) ◽  
pp. 227-235
Author(s):  
O. Litovchenko ◽  
◽  
I. Perova ◽  
Keyword(s):  
Electromagnetic Radiation ◽  
Negative Impact ◽  
Biological Effects ◽  
The Body ◽  
Combined Effect ◽  
Male Rats ◽  
Control Group ◽  
Prevention Measures ◽  
Immunological Parameters ◽  
Reduced Temperature

In modern conditions there is a high need to improve methods for establishing the nature of the combined influence of factors using modern approaches for further development of prevention measures against the negative impact of factors on the body. The purpose of the study was to investigate the combined effect of electromagnetic radiation and low temperatures with subsequent determination of the level of contribution of each factor in the formation of biological effects within the experiment by mathematical analysis of data using the method of artificial intelligence. Materials and methods. The subchronic experiment (30 days) was performed on male rats. The animals were divided into groups: combined exposure to electromagnetic radiation (70 kHz, 600 V/m) and reduced temperature (40C), isolated exposure to reduced temperature, isolated exposure to electromagnetic radiation and a control group. The establishment of biological effects was performed at stages 5, 15 and 30 days of the experiment. Changes in the body were assessed by physiological, biochemical and immunological parameters of the blood (30 indicators). Data processing was performed using computational intelligence methods (Neuro-fuzzy system). Results and discussion. The combined effect of the factors was manifested by the following biological effects: intensification of peroxidation processes with simultaneous moderate suppression of antioxidant protection, intensification of lipid metabolism was characterized by dyslipoproteinemia, manifesting in: increase in triglycerides, cholesterol and its fractions. Changes of the immune system were decrease in the ability of neutrophils to phagocytosis at different stages of the experiment, imbalance of the activation of the system of reducing the concentration of C4 at the background of increased intensity of IgM and IgG synthesis. The method of determining the intensity of the factors, developed on the basis of factor and cluster analysis, allowed to determine that during the experiment the ratios between the factors changed, but the greatest contribution was made by reduced temperature by both biochemical parameters (63%) and immunological (53%) at the same time, the level of electromagnetic radiation contribution was 37% and 47%, respectively. Thus, the reduced temperature was the leading factor in the combined effect, which added 60% to the overall biological effect throughout the study, against the effect of electromagnetic radiation for which the level of contribution was only 40%. By means of the factor analysis informative indicators, making it possible to establish the characteristics of biological effects, and therefore leading indicators in formation of biological effects were the increase in concentration of DC with simultaneous decrease in concentration of SH-groups, increase in VLDL and suppression of oxygen-dependent metabolism of neutrophil-test. Conclusion. Thus, the tested mathematical approach allowed to determine the leading role of each of the studied factors under the conditions of their combined influence in different systems, which allows to predict probable appropriate reactions of the whole organism and gives a reasonable approach to the development of preventive measures

Protective effects of dietary omega-3 fatty acid supplementation on organophosphate poisoning

2017 ◽  
Vol 34 (2) ◽  
pp. 69-82 ◽  
Author(s):  
Bahattin Avci ◽  
S. Sirri Bilge ◽  
Gokhan Arslan ◽  
Omer Alici ◽  
Ozge Darakci ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
The Body ◽  
Control Group ◽  
Organophosphate Poisoning ◽  
Protective Effects ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Omega 3 Fatty Acid ◽  
Locomotor Activities

In this study, we aimed to study the possible preventive effect of docosahexaenoic acid (DHA), a dietary omega-3 fatty acid, on toxicity caused by chlorpyrifos (CPF). Six groups of Sprague Dawley rats (200–250 g) consisting of equal numbers of males and females (n = 8) were assigned to study. The rats were orally given for 5 days. The control group was administered pure olive oil, which was the vehicle for CPF. The CPF challenge groups were administered oral physiological saline, pure olive oil, or DHA (50, 100 and 400 mg/kg dosages) for 5 days. The animals were weighed on the sixth day and then administered CPF (279 mg/kg, subcutaneously). The rats were weighed again 24 h following CPF administration. The body temperatures and locomotor activities of the rats were also measured. Blood samples, brain and liver tissues were collected for biochemical, histopathological and immunohistochemical examinations. A comparison with the control group demonstrated that CPF administration increased malondialdehyde (MDA) levels in blood, brain and liver, while it reduced catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations ( p < 0.05–0.001). Advanced oxidation protein products (AOPPs) increased only in the brain ( p < 0.001). DHA reduced these changes in MDA and AOPP values ( p < 0.05–0.001), while it increased CAT, SOD and GPx concentrations ( p < 0.05–0.001). Similarly, DHA prevented the decreases in body weight, body temperature and locomotor activities caused by CPF at 100 mg/kg and 400 mg/kg dosages ( p < 0.05–0.001). Similar to the physiological and biochemical changes, the histopathological damage scores, which increased with CPF ( p < 0.05–0.01), decreased at all three dosages of DHA ( p < 0.05–0.01). Our findings suggest that DHA, by supporting the antioxidant mechanism, reduces toxicity caused by CPF.

Effects of Lead Nitrate Induced Histological Changes in Liver of Male Sprague Dawley Rats

2021 ◽  
Vol 15 (11) ◽  
pp. 2948-2950
Author(s):  
Sumaira Abbasi ◽  
Mushtaq Ahmad ◽  
Kaukab Anjum ◽  
Amaidah Mir ◽  
Ayesha Irfan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Lead Nitrate ◽  
Normal Diet ◽  
The Body ◽  
Central Vein ◽  
Sprague Dawley ◽  
Toxic Agent ◽  
Sprague Dawley Rats ◽  
Group B

Lead is a highly toxic agent and a potent risk factor for various diseases as its quantity in an environment is increasing day by day. Aim: To observe and analyze the lead nitrate induced histomorphological changes in the liver of Sprague Dawley rats. Study Design: Experimental Study. Methodology: Animals of group A (control) were fed on normal diet but the animals of group B were given 50mg/kg of lead nitrate dissolved in 10ml of distilled water through oral gavage for 14 days daily. SPSS version 22 was used for data analysis. All the quantitative data was expressed as means ± SD. One Way ANOVA followed by Post Hoc Tukey test was applied. Results: Degenerative effects were noted. The number of Inflammatory and Kupfffer cells is increased with decreased in the body weight. Steatosis and central vein congestion were also present. Conclusion: It was concluded that degenerative effects histologically may be due to oxidative stress produced by formation of free radicals and denaturation of proteins by lead nitrate. Keywords: Lead, Liver, Central Vein Congestion, Oxidative Stress and Free Radicals.

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