scholarly journals Morphological changes of the Urediniospore of Puccinia kuehnii germ tube in function of temperature

2017 ◽  
Vol 3 (1) ◽  
pp. 19
Author(s):  
Claudinei Antonio Minchio ◽  
Lucas Henrique Fantin ◽  
Karla Braga de Oliveira ◽  
José A Rocha ◽  
Marcelo Giovanetti Canteri
Keyword(s):  
Orthogonal Polynomials ◽  
Factorial Design ◽  
Analysis Of Variance ◽  
Incubation Time ◽  
Germ Tube ◽  
Morphological Changes ◽  
Influence Of Temperature ◽  
Puccinia Kuehnii ◽  
Incubation Temperatures ◽  
Germ Tubes

The aim of this study was to evaluate changes in the morfology and length of the germ tube of Puccinia kuehnii, under the influence of temperature and incubation time. The development of the germ tube was asssesed for 24 hours. The concentration of uredospores was 2x105 esporos.ml-1 calibrated by a Neubauer chamber and plated in a 0.1 ml aliquot in water – agar (1.5%), placed in BOD and regulated at temperatures of 10, 20 and 30 °C. Readings were performed after 1, 3, 6, 12, 18 and 24 hours, with 5 replications. At the end of each period, germination was stopped by adding 0.1 ml of lactophenol. The length and morphology of 10 germinated spores per plate were evaluated with the help of a Motic Images MCCamera – Plus 2.0 ML software. Data were subjected to analysis of variance in a 3x6 factorial design with an interaction in orthogonal polynomials. The model estimated that a temperature of 20 °C, at 24 hours of incubation, was required to reach the maximum length. Germ tubes morphological changes were observed at incubation temperatures of 10, 20 and 30 °C showing zigzag, rectilinear and branched forms, respectively.

scholarly journals Assessing the Heamatology of Uda Rams Fed Graded Levels of Processed Cassava (Manihot esculentus L.) Peels

2021 ◽  
pp. 30-37
Author(s):  
M. M. Mika’ilu ◽  
A. A. Kwaido ◽  
S. A. Maigandi ◽  
I. M. Ribah ◽  
K. M. Aljameel ◽  
...  
Keyword(s):  
Factorial Design ◽  
Analysis Of Variance ◽  
Science And Technology ◽  
Processing Method ◽  
State University ◽  
Normal Range ◽  
Significant Difference ◽  
Feeding Trials ◽  
Cassava Peels ◽  
Hematological Values

The experiment was carried out at Kebbi State University of Science and Technology, Aliero using thirty two (32) yearlings Uda Rams in two feeding trials ran concurrently. Sixteen (16) rams were used in each experiment with four treatments replicated four times in a completely randomized factorial design (2 × 4). The animal represents the replicates while the processing method (drying and ensiling) and the level of inclusion represents the treatments respectively. The level of inclusion are 0, 10, 20 and 30% dried cassava peels (DCP) and ensiled cassava peels (ECP) respectively. Data were collected in each trial on hematological characteristics. Data generated was subjected to analysis of variance and least significant difference (LSD) was used to separate the means. Hematological values of rams fed DCP were within the normal range while those fed ECP were below the normal range. The results shows significant difference (P<0.05) between dried and ensiled method of processing in terms of haemoglobin, MCH, WBC and MCV. Rams fed dried cassava peels had lower haemoglobin and PCV compared to normal range. It was concluded that there was no significant difference between rams fed dried cassava peels and those fed ensiled cassava peels at 30% level of inclusion.

Heat Treatment Map of Al2Ca-added AlSi11MnMg Pressure-Cast Alloy Plotted by Full Factorial Design of Experiment and Analysis of Variance

2019 ◽  
Vol 61 (7-8) ◽  
pp. 511-516
Author(s):  
Gil-Yong Yeom ◽  
G. Eisaabadi B. ◽  
Shae K. Kim ◽  
Soong-Keun Hyun ◽  
Kyeong Suk Sim ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Factorial Design ◽  
Analysis Of Variance ◽  
Design Of Experiment ◽  
Cast Alloy ◽  
Full Factorial Design ◽  
Full Factorial

scholarly journals Optimization of Chitinase Production by Trichoderma virens in Solid State Fermentation Using Response Surface Methodology

2019 ◽  
Vol 967 ◽  
pp. 132-142
Author(s):  
Rachmawaty ◽  
Pagarra Halifah ◽  
Hartati ◽  
Zulkifli Maulana ◽  
Madihah Md. Salleh
Keyword(s):  
Factorial Design ◽  
Response Surface ◽  
Incubation Time ◽  
Physical Factor ◽  
Chitinase Activity ◽  
Optimal Value ◽  
Chitinase Production ◽  
Independent Variable ◽  
Screening Factor ◽  
Substrate Ph

Physical factor for chitinase production by Trichodermavirens was first carried out using screening factor of 2-level factorial. The design was employed by selecting incubation time, temperature, moisture substrate, pH, inoculums size and concentration ammonium sulphate as a model factors. The result of 2-level factorial design experiment showed thal all three independent variable have significant effect on chitinase production. The physical factor was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of three variables including various incubation time, temperature and moisture substrate were created using Design Expert® Software, Version 6.0.4 The design consist of 20 experiments, which include 6 replicate at center points. The optimal value for each variable are incubation time sixth days, temperature 27.83°C and moisture substrate 54% with predicted chitinase activity of 0.48738 U/g of dry substrate. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.48864 U/g of dry substrate, which is similar to the predicted value. The obtained value of the chitinase production was 0.48738 U/g IDS, which was 1.2 fold higher than that of the 2-level factorial design (0.261 U/gds)

The individual and combined influence of temperature, time, pH and COD concentration on the biodegradation activities of selected bacterial strains grown on raw Baker's yeast effluent

1994 ◽  
Vol 30 (12) ◽  
pp. 97-106
Author(s):  
M. Van Der Merwe ◽  
T.J. Britz
Keyword(s):  
Fatty Acid ◽  
Environmental Factors ◽  
Factorial Design ◽  
Incubation Time ◽  
Volatile Fatty Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Bacterial Strains ◽  
Data Set ◽  
Intermediary Metabolites

Five bacterial strains (Chryseomonas luteola, Fusobacterium mortiferum, Enterobacter agglomerans,Klebsiella oxytoca and an unidentified Gram-negative rod) were grown on raw baker's yeast effluent to assess the influence of environmental factors on biodegradation processes. A 3×4×3 factorial design was used to determine the effects of time, pH and COD concentration, at four different temperatures. Total volatile fatty acid production was chosen as the most representative indicator of biodegradation. Results showed that the strains differed greatly in their ability to produce anaerobic digester intermediary metabolites, under defined environmental conditions. The study showed that the degradation of the complex compounds in baker's yeast effluent could be enhanced by changing environmental factors. The most positive responses were obtained at the higher COD concentrations (30 g l−1), the higher pH values (6.0), after 24 to 48h incubation time and at the higher temperatures (35°C). The most positive effect (+355.00) was found forChryseomonas luteola at a 48h incubation time, COD concentration of 30 g l−1, pH of 6.0 and temperature of 35°C. The volatile fatty acid yields obtained by the strains differ from the statistical indications, but provide a valuable reference of the actual concentrations obtained during the experimental study. The factorial design represented the effects of environmental changes, while the quantitative TVFA data set gave experimental data. This study showed that the manipulation of various environmental factors in biologically controlled systems, such as anaerobic digesters, could further enhance the biodegradation efficiency of the microbial population in the raw effluent.

Ascospore germination, growth in culture, and imperfect spore formation in Cookeina sulcipes and Phillipsia crispata

1975 ◽  
Vol 53 (1) ◽  
pp. 56-61 ◽  
Author(s):  
J. W. Paden
Keyword(s):  
Germ Tube ◽  
Outer Wall ◽  
Wall Layer ◽  
Spore Formation ◽  
Spore Surface ◽  
No Formation ◽  
Ascospore Germination ◽  
Germ Tubes

Ascospores of Cookeina sulcipes germinate by one of two modes: (1) by the production of blastoconidia on sympodially proliferating conidiogenous cells which may arise from any point on the spore surface, and (2) by a thick polar germ tube. No ascospores were seen to germinate both ways. The conidiogenous cells are occasionally modified into narrow hyphae. The blastoconidia germinate readily but are evidently very short-lived. Ascospores of Phillipsia crispata germinate by two polar germ tubes; there is no formation of blastoconidia. In both species the inner ascospore wall separated from an outer wall layer during germination. In culture both C. sulcipes and P. crispata form arthroconidia. The arthroconidia are uninucleate; they germinate readily and reproduce the species when transferred to fresh plates.

Carbon dioxide induces endotrophic germ tube formation in Candida albicans

1990 ◽  
Vol 36 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Ruth C. Mock ◽  
Jordan H. Pollack ◽  
Tadayo Hashimoto
Keyword(s):  
Carbon Dioxide ◽  
Candida Albicans ◽  
Sodium Bicarbonate ◽  
Key Words ◽  
Germ Tube ◽  
Tube Formation ◽  
Chemical Inducers ◽  
Tube Emergence ◽  
Ph Range ◽  
Germ Tubes

Candida albicans formed germ tubes when exposed to air containing 5 to 15% carbon dioxide (CO2). The CO2-mediated germ tube formation occurred optimally at 37 °C in a pH range of 5.5 to 6.5. No germ tubes were produced at 25 °C, even when the optimal concentration of CO2 (10%) was present in the environment. The requirement of CO2 for germ tube formation could be partially substituted by sodium bicarbonate but not by N2. Carbon dioxide was required to be present throughout the entire course of germ tube emergence suggesting that its role is not limited to an initial triggering of morphogenic change. We suggest that carbon dioxide may be a common effector responsible for the germ tube promoting activity of certain chemical inducers for C. albicans. Key words: Candida albican germ tubes, CO2-induced germ tube formation, endotrophic germ tube formation.

Sign in / Sign up

Export Citation Format

Share Document