Photo-Crosslinkable Double-Network Hyaluronic Acid Based Hydrogel Dressing

2020 ◽  
Vol 982 ◽  
pp. 59-66
Author(s):  
Yu Long Ding ◽  
Hong Bo Zhang ◽  
Rui Xue Yin ◽  
Wen Jun Zhang
Keyword(s):  
Hyaluronic Acid ◽  
Biomedical Applications ◽  
Degradation Rate ◽  
In Vitro Degradation ◽  
Double Network ◽  
Biological Potential ◽  
Human Dermis ◽  
Enzymatic Degradability ◽  
Hdf Cells

Hyaluronic acid (HA)-based hydrogels are widely used in biomedical applications due to their excellent biocompatibility and enzymatic degradability. In this paper a photo-crosslinking double-network hyaluronic acid-based hydrogel dressing was proposed. Hyaluronic acid can be UV-crosslinked by modification with methacrylic anhydride (HA-MA) and disulfide-crosslinked by modification with 3,3'-dithiobis (propionylhydrazide) (DTP) (HA-SH). The mixings of these two materials at different ratios were produced. All the samples can be quickly gelled at 365 nm for 10 s. The rheological tests show that the storage modulus (G') of the double network (HA-SH/HA-MA) hydrogel is increased with the increase of HA-SH content. The HA-SH/HA-MA hydrogel has porous structure, high swelling ratio and Controlled degradation rate. In vitro degradation tests show that the ratio of HA-SH/HA-MA ratio was 9:1 (S9M1) in 100 U/ml hyaluronidase (Hase) degraded by 89.91±2.26% at 11d. The cytocompatibility of HA-SH/HA-MA hydrogels was proved by Live/Dead stainings and CCK-8 assays in the human dermis fibroblasts (HDF) cells test. All these results highlight the biological potential of the HA-SH/HA-MA hydrogels for DFU intervention.

Covalent Grafting of Poly(l-lactide) to Tune the In Vitro Degradation Rate

2007 ◽  
Vol 8 (8) ◽  
pp. 2492-2496 ◽  
Author(s):  
Martina Källrot ◽  
Ulrica Edlund ◽  
Ann-Christine Albertsson
Keyword(s):  
Degradation Rate ◽  
In Vitro Degradation ◽  
Covalent Grafting

Degradation Control of Collagen by Epigallocatechin-3-O-Gallate

2007 ◽  
Vol 342-343 ◽  
pp. 781-784 ◽  
Author(s):  
Han Hee Cho ◽  
Kazuaki Matsumura ◽  
Naoki Nakajima ◽  
Dong Wook Han ◽  
Sadami Tsutsumi ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Enzymatic Degradation ◽  
Egcg Treatment ◽  
Fibrous Protein ◽  
Gel Fraction ◽  
Tissue Culture Plate ◽  
Enzymatic Degradability ◽  
The Stability ◽  
Proliferation Assays

Stabilization of the fibrous protein collagen is important in biomedical applications. This study investigated the efficacy of degradation control of collagen using (-)-epigallocatechin-3-Ogallate (EGCG). EGCG treatment of collagen in solid state was carried out and collagen sponges produced were characterized by measuring the physicochemical properties such as gel fraction, the enzymatic degradability and cytocompatibility. According to gel fraction, EGCG-treated sponges showed the increase of insolubility compared to intact sponges. It showed that EGCG played a role in a crosslinker of collagen. Through in vitro enzymatic degradation test, EGCG-treated collagen sponges showed significant enhancement of resistance to collagenase in comparison with 25 mM EDC-treated collagen sponges. Also, cell proliferation assays showed that 40 mM EGCG-treated collagen sponges exhibited similar cytocompatibility properties compared with tissue culture plate. In summary, EGCG treatment of collagen sponges increased the stability of collagen. Therefore, crosslinking of collagen based scaffold with EGCG imparted more desirable properties, making it more applicable for use as a scaffold in tissue engineering applications.

scholarly journals Development of Nontoxic Biodegradable Polyurethanes Based on Polyhydroxyalkanoate and L-lysine Diisocyanate with Improved Mechanical Properties as New Elastomers Scaffolds

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1927 ◽  
Author(s):  
Cai Wang ◽  
Jiapeng Xie ◽  
Xuan Xiao ◽  
Shaojun Chen ◽  
Yiping Wang
Keyword(s):  
Tissue Regeneration ◽  
Biomedical Applications ◽  
Poly Ethylene Glycol ◽  
In Vitro Degradation ◽  
Elongation At Break ◽  
Molar Ratios ◽  
Soft Tissue Regeneration ◽  
Biodegradable Polyurethane ◽  
Poly Ethylene

A nontoxic and biodegradable polyurethane was prepared, characterized, and evaluated for biomedical applications. Stretchable, biodegradable, and biocompatible polyurethanes (LPH) based on L-lysine diisocyanate (LDI) with poly(ethylene glycol) (PEG) and polyhydroxyalkanoates(PHA) of different molar ratios were synthesized. The chemical and physical characteristics of the LPH films are tunable, enabling the design of mechanically performance, hydrophilic, and biodegradable behavior. The LPH films have a Young’s modulus, tensile strength, and elongation at break in the range of 3.07–25.61 MPa, 1.01–9.49 MPa, and 102–998%, respectively. The LPH films demonstrate different responses to a change of temperature from 4 to 37 °C, with the swelling ratio for the same sample at equilibrium varying from 184% to 151%. In vitro degradation tests show the same LPH film has completely different degradation morphologies in pH of 3, 7.4, and 11 phosphate buffered solution (PBS). In vitro cell tests show feasibility that some of the LPH films are suitable for culturing rat bone marrow stem cells (rBMSCs), for future soft-tissue regeneration. The results demonstrate the feasibility of the LPH scaffolds for many biomedical applications.

Silk Fibroin/Hyaluronic Acid Blend Film with Good Water Stability and Cytocompatibility

2013 ◽  
Vol 377 ◽  
pp. 209-214
Author(s):  
Ling Shuang Wang ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Cell Proliferation ◽  
Hyaluronic Acid ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Water Resistance ◽  
High Water ◽  
Casting Method ◽  
Blend Ratio ◽  
Blend Films

Stimulating cell proliferation is a challenge in the field of silk fibroin-based biomaterials. In this study, silk fibroin/hyaluronic acid blend films were prepared by a casting method using carbodiimide as a cross-linking agent. Carbodiimide induced silk fibroin to form Silk I crystal structure which was not affected by the presence of hyaluronic acid. The films showed high water resistance. In vitro, the performance of these films was assessed by seeding L929 cells. The results indicated that the silk fibroin/hyaluronic acid blend films with the blend ratio of 80/20 and 60/40 promoted cell proliferation compared with the pure silk fibroin or hyaluronic acid film. These results suggest that silk fibroin/hyaluronic acid blend films are water stable and cytocompatible materials which are expected to be useful in biomedical applications.

scholarly journals IN VITRO DEGRADATION OF POLYDIOXANONE (PDO) LIFTING THREADS IN HYALURONIC ACID (HA).

2019 ◽  
Vol 10 (1) ◽  
pp. 1-13
Author(s):  
Dubraska Suárez ◽  
Gladys J Velazco de Maldonado ◽  
Reynaldo Ortiz ◽  
Victor Garcia-Guevara ◽  
Blanca Miller-Kobisher
Keyword(s):  
Hyaluronic Acid ◽  
Key Words ◽  
Structural Changes ◽  
Hydrolytic Degradation ◽  
Central Core ◽  
In Vitro Degradation ◽  
Central Column ◽  
Rate Of Hydrolysis

Background: Recently, some clinicians have proposed implanting PDO threads imbibed in hyaluronic acid (HA). However, this is controversial since PDO sutures are hydrophilic and the presence of HA could increase the rate of hydrolysis. Aim: To demonstrate the degradation of PDO lifting threads in HA through ultramicroscopy. Materials and methods: Three, one cm long, segments of 23G PDO threads, where immersed in 1.5 ml non-crosslinked hyaluronic acid in previously labeled, sterile microcentrifuge tubes. These were observed by ultramicroscopy at 4x and 10x after 24, 48 and 72 hours. Results: Microphotographs taken after 24 hours already show structural changes in the fibers, presenting an increase in interlaminar spaces and dilution of violet pigmentation. At 48 hours, degradation continues. PDO hygroscopy is observed as aqueous content between the peripheral layers and the central core of the thread. Some fibers show breakage, and there is an increase in interlaminar and interfibrillar spaces. At 72 hours, as the pigment is released, larger empty spaces are observed in the central column of the thread, and there is disorganization of the peripheral fibrils with fraying all along the fiber. Conclusions: Hyaluronic acid induces rapid biodegradation of the PDO thread by hydrolysis beginning 24 hours after contact of the thread with the biomaterial. We hypothesize that non-crosslinked hyaluronic acid is a powerful catalyzing agent for hydrolytic degradation of the PDO thread, since this thread is highly hydrophilic. Thus, we suggest that clinically embedding PDO threads with HA will only accelerate biodegradation of the suture. Key words: Lifting threads, polydioxanone, hyaluronic acid, biodegradation, PDO hydrolysis

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