scholarly journals In vitro and In vivo Antifungal Activity of Culture Filtrates and Organic Extracts of Penicillium sp. and Gliocladium spp. against Botrytis cinerea

2017 ◽  
Vol 8 (12) ◽  
Author(s):  
Hassine M ◽  
Jabnoun Khiareddine H ◽  
Aydi Ben Abdallah R ◽  
Daami Remadi M
Keyword(s):  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Penicillium Sp ◽  
Culture Filtrates ◽  
Organic Extracts ◽  
In Vivo Antifungal Activity

In vitro and in vivo antifungal activity and preliminary mechanism of cembratrien-diols against Botrytis cinerea

2020 ◽  
Vol 154 ◽  
pp. 112745
Author(s):  
Qiong Yang ◽  
Jiao Wang ◽  
Peng Zhang ◽  
Shengnan Xie ◽  
Xiaolong Yuan ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
In Vivo Antifungal Activity

scholarly journals In Vitro and In Vivo Antifungal Activity of AmBisome Compared to Conventional Amphotericin B and Fluconazole against Candida auris

2021 ◽  
Author(s):  
Janet Herrada ◽  
Ahmed Gamal ◽  
Lisa Long ◽  
Sonia P. Sanchez ◽  
Thomas S. McCormick ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Kidney Tissue ◽  
Treated Group ◽  
Untreated Group ◽  
Candida Auris ◽  
Fungal Burden ◽  
In Vivo Antifungal Activity

Antifungal activity of AmBisome against Candida auris was determined in vitro and in vivo. AmBisome showed MIC50 and MIC90 values of 1 and 2 μg/mL, respectively. Unlike conventional amphotericin B, significant in vivo efficacy was observed in the AmBisome 7.5 mg/kg -treated group in survival and reduction of kidney tissue fungal burden compared to the untreated group. Our data shows that AmBisome shows significant antifungal activity against C. auris in vitro as well as in vivo.

IN VITRO AND IN VIVO ANTIFUNGAL ACTIVITY OF EIGHT MEDICINAL PLANTS AGAINST CITRUS SOUR ROT AGENT GEOTRICHUM CANDIDUM

2015 ◽  
pp. 1563-1570 ◽  
Author(s):  
Idriss Talibi ◽  
Latifa Askarne ◽  
Hassan Boubaker ◽  
El Hassane Boudyach ◽  
Abdellah Ait Ben Oumar
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Geotrichum Candidum ◽  
Sour Rot ◽  
Citrus Sour Rot ◽  
In Vivo Antifungal Activity

scholarly journals In vitro and in vivo antifungal activity of natural inhibitors against Penicillium expansum

2013 ◽  
Vol 33 ◽  
pp. 40-46 ◽  
Author(s):  
Claudia Fieira ◽  
Francieli Oliveira ◽  
Rubens Perez Calegari ◽  
Alessandra Machado ◽  
Alexandre Rodrigo Coelho
Keyword(s):  
Antifungal Activity ◽  
Penicillium Expansum ◽  
Natural Inhibitors ◽  
In Vivo Antifungal Activity

scholarly journals Evaluation of the In Vitro and In Vivo Antifungal Activity of APX001A/APX001 Against Candida auris

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S471-S472
Author(s):  
Emily Larkin ◽  
Lisa Long ◽  
Christopher Hager ◽  
Karen Joy Shaw ◽  
Mahmoud Ghannoum
Keyword(s):  
Antifungal Activity ◽  
Candida Auris ◽  
In Vivo Antifungal Activity

scholarly journals Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9626
Author(s):  
Huiyu Hou ◽  
Xueying Zhang ◽  
Te Zhao ◽  
Lin Zhou
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Botrytis Cinerea ◽  
Spore Germination ◽  
Gray Mold ◽  
Origanum Vulgare ◽  
Mycelium Growth ◽  
Main Components

Background Botrytis cinerea causes serious gray mold disease in many plants. This pathogen has developed resistance to many fungicides. Thus, it has become necessary to look for new safe yet effective compounds against B. cinerea. Methods Essential oils (EOs) from 17 plant species were assayed against B. cinerea, of which Origanum vulgare essential oil (OVEO) showed strong antifungal activity, and accordingly its main components were detected by GC/MS. Further study was conducted on the effects of OVEO, carvacrol and thymol in vitro on mycelium growth and spore germination, mycelium morphology, leakages of cytoplasmic contents, mitochondrial injury and accumulation of reactive oxygen species (ROS) of B. cinerea. The control efficacies of OVEO, carvacrol and thymol on tomato gray mold were evaluated in vivo. Results Of all the 17 plant EOs tested, Cinnamomum cassia, Litsea cubeba var. formosana and O. vulgare EOs had the best inhibitory effect on B. cinerea, with 0.5 mg/mL completely inhibiting the mycelium growth of B. cinerea. Twenty-one different compounds of OVEO were identified by gas chromatography–mass spectrometry, and the main chemical components were carvacrol (89.98%), β-caryophyllene (3.34%), thymol (2.39%), α-humulene (1.38%) and 1-methyl-2-propan-2-ylbenzene isopropyl benzene (1.36%). In vitro experiment showed EC50 values of OVEO, carvacrol and thymol were 140.04, 9.09 and 21.32 μg/mL, respectively. Carvacrol and thymol completely inhibited the spore germination of B. cinerea at the concentration of 300 μg/mL while the inhibition rate of OVEO was 80.03%. EC50 of carvacrol and thymol have significantly (P < 0.05) reduced the fresh and dry weight of mycelia. The collapse and damage on B. cinerea mycelia treated with 40 μg/mL of carvacrol and thymol was examined by scanning electron microscope (SEM). Through extracellular conductivity test and fluorescence microscope observation, it was found that carvacrol and thymol led to increase the permeability of target cells, the destruction of mitochondrial membrane and ROS accumulation. In vivo conditions, 1000 μg/mL carvacrol had the best protective and therapeutic effects on tomato gray mold (77.98% and 28.04%, respectively), and the protective effect was significantly higher than that of 400 μg/mL pyrimethanil (43.15%). While the therapeutic and protective effects of 1,000 μg/mL OVEO and thymol were comparable to chemical control. Conclusions OVEO showed moderate antifungal activity, whereas its main components carvacrol and thymol have great application potential as natural fungicides or lead compounds for commercial fungicides in preventing and controlling plant diseases caused by B. cinerea.

Biological control of Botrytis cinerea using the antagonistic and endophytic Burkholderia cepacia Cs5 for vine plantlet protection

2011 ◽  
Vol 57 (11) ◽  
pp. 896-901 ◽  
Author(s):  
Olfa Kilani-Feki ◽  
Samir Jaoua
Keyword(s):  
Biological Control ◽  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Burkholderia Cepacia ◽  
Bacterial Biomass ◽  
Endophytic Bacterium ◽  
Bacterial Suspension ◽  
Self Defense

Antifungal activity of the Burkholderia cepacia Cs5 was tested in vitro and in vivo for the control of Botrytis cinerea . Bacterial biomass was significantly improved by the amendment of ZnSO4, Mo7(NH4)6O24, and mannitol to the NBY medium; consequently, the amount of the secreted fungicides was increased. The quantification of B. cinerea inhibition, in liquid and solid conditions, showed an important sensitivity of this fungus to the strain Cs5 fungicides. Microscopic monitoring impact of these fungicides on mycelium structure showed an important increase in their diameter and ramifications in the presence of 0.75% supernatant. For the in vivo application of the strain Cs5, Vitis vinifera plantlets were inoculated with a Cs5 bacterial suspension, then with B. cinerea spores. The plantlets protection was total and durable when these two inoculations were made 3 weeks apart, which is the time for the endophytic bacterium to colonize the plantlets up to the top leaves. This protection is due to Cs5 antagonism and the elicitation of the plantlets self-defense via the root overgrowth.

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