scholarly journals Karakterisasi sekuen DNA gen mtCO-1 hama penggerek pucuk (Hypsipyla sp.) pada tanaman mahoni (Swietenia macrophylla King) di Kabupaten Minahasa dan Kota Tomohon Provinsi Sulawesi Utara

2020 ◽  
Vol 17 (1) ◽  
pp. 53
Author(s):  
Jusuf Manueke ◽  
Jefry Sembiring ◽  
Dantje Tarore
Keyword(s):  
Cluster Analysis ◽  
Genetic Differences ◽  
Morphometric Character ◽  
Swietenia Macrophylla ◽  
North Sulawesi ◽  
Tree Construction ◽  
Pcr Method ◽  
Descriptive Method

<p><em> Hypsiphyla</em> sp. include the Order Lepidoptera, Family Pyralidae. These insects is one of the important pests on mahogany plants (<em>Swietenia macrophylla </em>King.).  This pest has been found to attack <em>S. macrophylla</em> plants in North Sulawesi, especially in the area of mahogany plant centers namely North Minahasa Regency and Tomohon City. The aim of the study was to determine the CO1 gen profile of mahogany shoots borer (<em>Hypsipyla</em> sp.) on mahogany plant (<em>Swietenia macrophylla</em>) in Minahasa Regency and Tomohon City, North Sulawersi Province. The method used is descriptive method, consists of extraction or purification of total double strands DNA (dsDNA), amplification of CO1 gene by PCR method, visualization of PCR results with atomatic electrophoresis qiagen, and sequencing. The sequenced data that is the construction of the phylogeny tree are described according to clusters which give the morphometric character of each sample. The results of the analysis of genetic differences between <em>Hypsipyla</em> sp. origin of North Minahasa and Tomohon namely 3.8%–4.4%. <em>Hypsipyla</em> sp. origin of North Minahasa and Tomohon with <em>H. grandella</em> namely 9.0%–9.3%. Based on the results of this analysis it can be seen that the kinship of <em>Hypsipyla</em> sp. in North Minahasa Regency and Tomohon City it is closer to <em>H. robusta </em>than <em>H. grandella</em>. Results of cluster analysis of <em>Hypsipyla</em> sp. in North Minahasa Regency and Tomohon City, North Sulawesi Province compared to <em>H. robusta</em> and <em>H. grandella</em> in the Gen Bank mapped in the phylogeny tree construction indicate that <em>Hypsipyla</em> sp. in North Mihasa Regency and Tomohon City, North Sulawesi Province already exists or forms a separate clade with <em>H. robusta</em> and <em>H. grandella</em> clades in Bank Gen.</p>

scholarly journals PROFIL PERIKANAN TUNA DAN CAKALANG DI INDONESIA

2019 ◽  
Vol 4 (1) ◽  
pp. 23
Author(s):  
Maulana Firdaus
Keyword(s):  
Statistical Data ◽  
Secondary Data ◽  
Fishing Ground ◽  
Skipjack Tuna ◽  
North Sulawesi ◽  
Long Line ◽  
West Sumatra ◽  
Arafura Sea ◽  
Scientific Report ◽  
Descriptive Method

Indonesia memegang peranan penting dalam perikanan Tuna, Tongkol dan Cakalang di dunia. Indonesia telah memasok lebih dari 16% produksi Tuna, Tongkol dan cakalang dunia. Tuna dan cakalang memiliki peranan penting bagi sektor perikanan tangkap di Indonesia sehingga pengetahuan tentang profil perikanan Tuna dan cakalang menjadi sangat penting untuk diketahui. Kajian yang dilakukan pada tahun 2017 ini bertujuan untuk mendeskripsikan profil perikanan Tuna dan cakalang di Indonesia yang akan dikemukakan berdasarkan penelurusan data sekunder berupa data statistik, laporan penelitian dan publikasi ilmiah terkait perikanan Tuna dan cakalang di Indonesia yang dianalisis secara deskriptif. Hasil kajian menunjukkan bahwa perikanan Tuna dan cakalang di Indonesia terdiri jenis yaitu industri dan artisanal. Daerah penyebaran ikan Tuna dan  cakalang meliputi  Laut Banda, Laut Maluku, Laut Flores, Laut Sulawesi, Laut Hindia, Laut Halmahera, perairan utara Aceh, barat Sumatera, selatan Jawa, utara Sulawesi, Teluk Tomini,  Teluk  Cendrawasih  dan  Laut  Arafura. Produksi Tuna dan cakalang terus meningkat sejak tahun 2000 sampai dengan tahun 2015. Peningkatan produksi Tuna dan cakalang menunjukkan bahwa tingginya tingkat permintaan terhadap kedua komoditas tersebut. Alat tangkap yang digunakan untuk menangkap Tuna dan cakalang sangat beragam yang dapat dikelompokkan menjadi 6 jenis, yaitu rawai Tuna (Tuna long line), rawai hanyut selain rawai Tuna (drift longline other than Tuna long line), rawai tetap (set long line), huhate (skipjack pole and line), pancing tonda (troll line) dan pancing yang lain (other pole and line)Title: The Profile of Tuna and Cakalang Fishery in IndonesiaIndonesia plays an important role in Tuna and Skipjack fisheries in the world due to its supply of  more than 16% of the world’s Tuna and skipjack production. Since they have been being a vital commodity in capture fisheries in Indonesia, it is important to have knowledge of Tuna and skipjack fisheries. This study was conducted in 2017 and it aims to describe the profile of Tuna and skipjack in Indonesia that built upon secondary data. The data were collected from statistical data, scientific report and publication related to Tuna and skipjack fisheries in Indonesia and they were analyzed using descriptive method. The results suggest that Tuna and skipjack fisheries in Indonesia consist of industrial and artisanal types. The fishing ground of Tuna and skipjack covering Banda Sea, Maluku Sea, Flores Sea, Sulawesi Sea, Indian Ocean, Halmahera Sea, Northern Aceh Sea, West Sumatra, South Java, North Sulawesi, Tomini Bay, Cendrawasih Bay and Arafura Sea. The production of Tuna and skipjack Tuna continues to increase particularly during 2000 to 2015. The increased number of Tuna and skipjack production indicates the high demand of these two commodities. There are various fishing gear to catch Tuna and Skipjack, which can be grouped into 6 types, namely Tuna long line, drift longline other than Tuna long line, set long line, huhate (skipjack pole and line), troll line and other fishing rods (other pole and line)

scholarly journals ANALISIS TINGKAT PENDAPATAN MASYARAKAT NELAYAN SOMA PAJEKO DI KECAMATAN SALIBABU KABUPATEN KEPULAUAN TALAUD SULAWESI UTARA

2018 ◽  
Vol 6 (11) ◽  
Author(s):  
Siska Salatan ◽  
Victoria E.N. Manoppo ◽  
Suria Darwisito
Keyword(s):  
Secondary Data ◽  
Income Level ◽  
The Philippines ◽  
Economic Life ◽  
Purse Seine ◽  
Net Income ◽  
Community Group ◽  
North Sulawesi ◽  
Descriptive Method ◽  
Spatial Plan

AbstractTalaud Islands Regency includes maritime territory, 94.6% of which is waters directly adjacent to the Philippines and holds a variety of high economic living resources. In North Sulawesi Provincial Regulation Numbered 1/2017, article 12 concerning Zoning Plan of Coastal Zone and Small Islands of North Sulawesi Province 2017-2037, Talaud Islands Regency is designated as Integrated Marine and Fisheries Center Location of Talaud. It is located in Salibabu district, where Regional Regulation of Spatial Plan Numbered 1/2014 concerning Spatial Plan of Talaud Islands Regency for 2014-2034, Salibabu District is prioritized for Fishing Fisheries Regional Development and Management since its unutilized fisheries potential is still about 23,104 tons/year. In 2017, Salibabu district occupies the highest order for total fisheries production in Talaud Islands District. It was 702.3 tons or 11.79% of that in Talaud Islands Regency, 46.23% of which were gained from purse seines. Majority of the purse seine fishermen are those whose main livelihoods are fishermen and do not have other jobs. Fishing community is, in general, a relatively lagging community group socially, economically and culturally when compared with other community groups. The purse seine fishermen community in Salibabu district, in fact, still has mean income far below the minimum wage of Kabupaten Talaud, IDR. 1,500,000, -. The purse seine fisherman community in Salibabu district is still chained with poverty and backwardness. Therefore, there are needs for external intervention as an incentive to empower them in order to get out of the situation. This study used descriptive method, a method of studying the recent status of human groups, an object, a set of condition, a system of thinking or a class of events. Data collection consisted of primary and secondary data. It was done through in-depth interviews, filling questionnaires and literature studies. Respondents were purse seine fishermen communities in Salibabu district. Data analysis used descriptive method to know the socio-economic life of the communities and income formula to analyze the income level of fishermen purse seine. Results showed that mean net income in Salibabu district was approximately IDR 86,784,000/year for the purse seine owner and IDR.10,608,840/year for fishing crews, respectively, with main catches of skipjack tuna, mackerels, and scads. Keywords: Purse seine fisherman, Salibabu District and Income level of Fisherman AbstrakKabupaten Kepulauan Talaud termasuk wilayah bahari dimana 94,6% wilayahnya adalah perairan yang berbatasan langsung dengan negara Philipina dan memiliki sumberdaya hayati yang bernilai ekonomis tinggi. Dalam Peraturan Daerah Provinsi Sulawesi Utara No. 1 tahun 2017 pasal 12 tentang Rencana Zonasi Wilayah Pesisir dan Pulau-Pulau Kecil Provinsi Sulawesi Utara tahun 2017-2037, Kabupaten Kepulauan Talaud ditetapkan sebagai lokasi Sentra Kelautan dan Perikanan Terpadu. Lokasi tersebut terletak di Kecamatan Salibabu, dimana pada Perda RTRW (Rencana Tata Ruang Wilayah) No. 1 Tahun 2014 tentang Rencana Tata Ruang Wilayah Kab. Kepulauan Talaud Tahun 2014-2034, Kecamatan Salibabu diprioritaskan untuk Pengembangan dan Pengelolaan Kawasan Perikanan Tangkap karena potensi perikanan yang belum termanfaatkan ± 23.104 Ton/Tahun. Tahun 2017, Kecamatan Salibabu menempati urutan tertinggi untuk total produksi perikanan di Kabupaten Kepulauan Talaud. Total produksi perikanan Kecamatan Salibabu sebesar 702,3 ton atau 11,79 % dari keseluruhan produksi perikanan di Kabupaten Talaud. Dari total produksi tesebut 46,23 merupakan hasil produksi usaha soma pajeko. Mayoritas nelayan soma pajeko merupakan nelayan penuh yang mata pencaharian utamanya sebagai nelayan dan tidak memiliki pekerjaan lain. Masyarakat nelayan pada umumnya merupakan kelompok masyarakat yang relatif tertinggal secara sosial, ekonomi dan budaya bila dibandingkan dengan kelompok masyarakat lainnya. Demikian juga berlaku untuk masyarakat nelayan soma pajeko di Kecamatan Salibabu dimana penghasilan nelayan dibawah upah minimum Kabupaten kepulauan Talaud sebesar Rp. 1.500.000,-. Masyarkat nelayan soma pajeko di Kecamatan Salibabu masih terbelenggu oleh kemiskinan. Oleh karena itu dengan maksud bisa keluar dari kondisi tersebut perlu ada intervensi ekternal sebagai suatu dorongan untuk memberdayakan mereka.Penelitian ini menggunakan metode deskriptif, yaitu suatu metode dalam meneliti sekelompok manusia, suatu objek, suatu set kondisi, suatu system pemikiran ataupun suatu kelas peristiwa pada masa sekarang. Jenis data yang dikumpulkan terdiri dari data primer dan data sekunder. Pengumpulan data dilakukan melalui wawancara secara mendalam terhadap responden, pengisian kuesioner dan studi kepustakaan. Responden terdiri dari masyarakat nelayan soma pajeko di Kecamatan Salibabu. Analisis data menggunakan metode deskriptif untuk mengetahui kehidupan sosial ekonomi masyarakat dan analisis mengunakan rumus pendapatan digunakan untuk menganalisis tingkat pendapatan masyarakat nelayan soma pajeko.Hasil penelitian menunjukkan bahwa pendapatan bersih juragan soma pajeko di Kecamatan Salibabu rata- rata berkisar Rp86.784.000,-/tahun dan Rp. 10.608.840,-/tahun untuk pendapatan bersih masanae dengan hasil tangkapan utama berupa ikan Cakalang, Ikan Layang dan Ikan Tongkol.Kata kunci: Nelayan Soma Pajeko , Kecamatan Salibabu dan Tingkat Pendapatan

scholarly journals The Uses of Cempaka Wood (Magnolia spp) by the Community in Rumoong Atas Village North Sulawesi, Indonesia and Its Environmental Impact

2021 ◽  
Vol 317 ◽  
pp. 01098
Author(s):  
Irawan Arif ◽  
Kismartini ◽  
Hidayat Jafron Wasiq ◽  
Abdulah Lutfy ◽  
Suryaningsih Rahma ◽  
...  
Keyword(s):  
Environmental Impact ◽  
Natural Forests ◽  
Field Surveys ◽  
The Public ◽  
North Sulawesi ◽  
Critical Areas ◽  
Environmental Friendly ◽  
The People ◽  
The Village ◽  
Descriptive Method

The uses of cempaka wood to satisfy the demand in constructing houses and household furniture is considered as environmental-friendly doings which can reinforce the efforts of preventing global warming. Cempaka is commonly used by the people in North Sulawesi. This study aims to discover the use of cempaka wood in Rumoong Atas Village, North Sulawesi and its environmental impact. The methods employed are descriptive method based on the interview results from the participants in Rumoong Atas Village and field surveys. The findings indicate that there are various usages of cempaka wood in the village. The villagers prefer cempaka woods because of hereditary uses and the level of cultural historical attachment, excellent timber quality and texture, and the availability. These diverse functions contain implications for abundant existence of cempaka plants around Rumoong Atas Village. The survival of cempaka plants must be maintained and developed continuously because of their varied functions in Rumoong Atas area. The enhancement of these plants by the public of Rumoong Atas is expected to preserve the existence of natural forests in North Sulawesi which have been declining in both quality and quantity and deliver significant influence towards additional width of critical areas.

scholarly journals Karakteristik Fisiologi Daun Aren Varietas Akel Toumuung

Buletin Palma ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 49
Author(s):  
NURHAINI MASHUD ◽  
FARIDA OCTAVIA
Keyword(s):  
Chlorophyll Content ◽  
Mechanical Damage ◽  
Coconut Palm ◽  
Font Size ◽  
Fresh Weight ◽  
Total Chlorophyll Content ◽  
North Sulawesi ◽  
Descriptive Method ◽  
Palm Leaf ◽  
Physiological Characters

<p><span style="font-size: medium;">ABSTRAK </span></p><p>Apabila ditinjau dari karakter fisiologi, tanaman aren memiliki karakter yang sangat berbeda dengan kelapa atau pinang  walaupun ketiga tanaman ini termasuk famili palma. Daun sebagai organ fotosintetik memiliki bermacam-macam  pigmen aseptor elektron yang mendukung proses fotosintesis, antara lain klorofil. Selain itu, dalam daun terdapat stomata yang berfungsi sebagai alat adaptasi tanaman terhadap cekaman kekeringan, dan trikoma yang berfungsi  sebagai pelindung dari kerusakan mekanis yang telah terbukti pada tanaman kelapa. Penelitian tentang karakter  fisiologis daun tanaman aren varietas Akel Toumuung dilakukan dengan tujuan mempelajari karakter-karakter fisiologis  daun, yaitu  klorofil, stomata dan trikoma. Penelitian dilakukan di Desa Kumlembuai, Pinaras dan Walian,  Kotamadya  Tomohon, Provinsi Sulawesi Utara pada bulan Juni 2012, menggunakan metode deskriptif. Untuk analisis karakteristik  fisiologis, contoh  daun diambil pada daun nomor 14, kemudian dianalisis di laboratorium Ekofisiologi, Balai Penelitian  Tanaman Palma. Hasil penelitian menunjukkan bahwa kandungan klorofil total daun tanaman aren varietas Akel  Toumuung di Kumelembuai, Pinaras dan  Walian  berturut-turut adalah 1,89 mg/g berat segar, 2,18 mg/g berat segar  dan 1,85 mg/g berat segar klorofil a; 1,38 mg/g berat segar, klorofil a 1,80 mg/g berat segar, dan 1,04 mg/g berat segar;  serta klorofil b 0,69 mg/g berat segar, 0,82 mg/g berat segar  dan  0,67 mg/g berat segar. Jumlah stomata/mm2 berturut-turut adalah 24,78, 29,22 dan 29,11. Jumlah trikoma berturut-turut adalah 2,89, 5,67 dan 4,56. Kandungan klorofil dan  stomata daun aren kurang dari tanaman kelapa, tetapi jumlah trikomanya lebih banyak dari tanaman kelapa. Karakter fisiologis tanaman aren ini menggambarkan ciri tanaman yang tumbuh pada lahan yang ternaungi.</p><p>Kata kunci: Karakter fisiologi, klorofil, stomata, trikoma, daun aren.</p><p> </p><p><strong>Physiology Characteristic of Toumuung Sugar Palm Leaf</strong></p><p><span style="font-size: medium;">ABSTRACT </span></p><p>In term of physiology character, sugar palm has a very different character with coconut or arecanut although all three  plants including in palm family. Leaves as organ of photosynthetic have an assortment of electron acceptors pigment  that supports the process of photosynthesis, among others chlorophyll. In addition, there are stomata in the leaves,  which serves as a means of adaptation tool of plants to drought stress, and trichomes that serves as a protection from  mechanical damage that has been proven in the coconut palm. Research on the physiological characters of tall sugar  palm leaves was done to study the physiological characters of leaves, namely chlorophyll, stomata and trichomes. The  study was conducted in the villages of Kumelembuai, Pinaras and Walian, the Municipality of Tomohon, North Sulawesi Province in June 2012, using descriptive method. For the analysis of the physiological characteristics, leaf  samples were taken on leaf number 14, then analyzed in the Ecophysiology laboratory, Palma Research Institute. The  results showed that total chlorophyll, content of sugar palm in Kumelembuai, Pinaras and Walian was 1.89 mg/g fresh  weight, 2.18 mg/g fresh weight and 1.85 mg/g fresh weight; chlorophyll a is 1,38 mg/g fresh weight, 1,81 mg/g fresh  weight  dan 1,04 mg/g fresh weight; chlorophyll b is 0,69 mg/g fresh weight, 0,82 mg/g fresh weight and 0,67 mg/g  fresh weight respectively. Number of stomata/mm2 is 24.78, 29.22 and 29.11, respectively. Number of trichomes/mm2  were 2,89, 5,67 dan 4,56, respectively. Chlorophyll content and  the amount of  leaves stomata of sugar palm less than  coconut palm, but the amount of trichomes more than coconut palm. This sugar palm physiological characters  describe the plants that grow in the shaded area.</p><p>Keywords: Character physiology, chlorophyll, stomata, trichomes, palm leaf.</p>

scholarly journals ANALISIS PERBANDINGAN KONTRIBUSI DAN EFEKTIVITAS PAJAK BAHAN BAKAR KENDARAAN BERMOTOR DI PROVINSI GORONTALO DAN PROVINSI SULAWESI UTARA

2019 ◽  
Vol 14 (4) ◽  
Author(s):  
Miswati Dalonto ◽  
Jullie J. Sondakh ◽  
Steven J. Tangkuman
Keyword(s):  
Local Government ◽  
The Other ◽  
Government Revenue ◽  
North Sulawesi ◽  
Fuel Taxes ◽  
Qualitative Descriptive ◽  
Descriptive Method

The contribution and the effectiveness of motorcycle fuel taxas is one of the tools that can measure how far the contribution of motorcycle fuel taxes towards original local government revenue and also how far the achievements level of tax collaction by local government in regards of motorcycle fuel taxes because of tax incomes is very crucial to build a district. This study aims to (1) Determine how big the contribution motorcycle fuel taxes toword Original government revenue in Gorontalo Province and North Sulawesi Province (2) To Determine how big the effectiveness motorcycle fuel taxes in Gorontalo Province and North Sulawesi Province (3) How the comparison of contribution and the effectiveness of motorcycle fuel taxes in Gorontalo Province and North Sulawesi Province. This study is using qualitative descriptive method. The result of this study is showing that the motorcycle fuel taxes in Gorontalo Province is stil lack of giving contribution because of the result shows that the average of percentage is 19,29%, and on the other side, North Sulawesi Province also shows a lack of giving contribution which is 19,75% in percentage, and effectiveness motorcycle fuel taxes in Gorontalo Province and North Sulawesi Province is included as very effective criteria by showing a result of criteria level is more than 100%.

scholarly journals Malaria Detection using Polymerase Chain Reaction (PCR) Method in Pregnant Women’s Saliva on Several Hospitals in North Sulawesi Province

2016 ◽  
Author(s):  
Dewanto Khrisnamurti
Keyword(s):  
Pregnant Women ◽  
First Trimester ◽  
Cross Sectional Study ◽  
Malaria In Pregnancy ◽  
Cross Sectional ◽  
North Sulawesi ◽  
Blood Smears ◽  
Pcr Method ◽  
Polymerase Chain ◽  
In Pregnancy

Objective: To detect malaria by PCR examination of saliva in pregnant women and to obtain the incidence of malaria and the type of plasmodium causing malaria in pregnant women at various hospital in North Sulawesi. Method: A descriptive cross sectional study in pregnant women during antenatal care at the Department of Obstetrics and Gynecology Faculty of Medicine University of Sam Ratulangi/Prof. Dr. R.D. Kandou General Hospital in Manado, R.W. Monginsidi Hospital in Manado, Bethesda Hospital in Tomohon, and Datoe Binangkang Hospital in Kotamobagu, from 1 April until 31 May 2008. Result: There were 43 pregnant women clinically diagnosed with malaria, 23 (53.49%) by PCR examination of saliva and 20 (46.59%) by blood smears. From 23 cases of malaria in pregnancy detected by PCR, there were 18 diagnosed as tropical malaria, 3 tertian malaria, and 2 mixed malaria. Conclusions: The incidence of malaria in pregnancy at various hospital in North Sulawesi using PCR methods for saliva examination from April 1 - May 31 2008 is 53.49%. In this study malaria are mostly caused by Plasmodium falciparum with the largest incidence in primigravida, in the first trimester. [Indones J Obstet Gynecol 2012; 36-1:14-9] Keywords: malaria, PCR, pregnancy, saliva

scholarly journals Discrimination and Genetic Diversity of Cephalotaxus Accessions Using AFLP Markers

2000 ◽  
Vol 125 (4) ◽  
pp. 404-412 ◽  
Author(s):  
Donglin Zhang ◽  
Michael A. Dirr ◽  
Robert A. Price
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Shade Tolerance ◽  
Fragment Length Polymorphism ◽  
Genetic Differences ◽  
Aflp Markers ◽  
Base Pairs ◽  
Aflp Data ◽  
Hybrid Species ◽  
Sun And Shade

Cephalotaxus Sieb. and Zucc. (plum yew) species and cultivars have become popular because of their sun and shade tolerance, resistance to deer browsing, disease and insect tolerance, and cold and heat adaptability. Unfortunately, the nomenclature and classification in the literature and nursery trade are confusing due to their extreme similarity in morphology. In this study, amplified fragment-length polymorphism (AFLP) markers were used to discriminate taxa and evaluate genetic differences among 90 Cephalotaxus accessions. A total of 403 useful markers between 75 and 500 base pairs (bps) was generated from three primer-pair combinations. Cluster analysis showed that the 90 accessions can be classified as four species, C. oliveri Mast., C. fortunei Hooker, C. harringtonia (Forbes) Koch., and C. ×sinensis (a hybrid species); four varieties, C. fortunei var. alpina Li, C. harringtonia var. koreana (Nakai) Rehd., C. harringtonia var. nana (Nakai) Hornibr., and C. harringtonia var. wilsoniana (Hayata) Kitamura; and eight cultivars. Suggested names are provided for mislabeled or misidentified taxa. The Cephalotaxus AFLP data serve as a guide to researchers and growers for identification and genetic differences of a taxon, and a model to establish a cultivar library against which later introductions or problematic collections can be cross-referenced.

Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene)

2019 ◽  
Vol 967 ◽  
pp. 71-82
Author(s):  
Alexius Mege Revolson ◽  
Yermia Semuel Mokosuli ◽  
Jacqueline Jochebed Rayer Debby ◽  
Ellen Hetie Adil ◽  
Christny Rompas ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Gene ◽  
Wild Pigs ◽  
North Sulawesi ◽  
Extraction And Purification ◽  
Gh Gene ◽  
Pcr Method ◽  
Local Pigs ◽  
Key Words Growth Hormone ◽  
Relationship Of

Growth hormone regulates reproduction and growth in mammals. A study was conducted to obtain the characteristics of the GH gene, in local pigs in North Sulawesi. Pig samples were obtained from traditional farmers, from four districts in North Sulawesi. DNA extraction and purification, using pig pituitary tissue. Amplification of GH gene, performed by PCR method. Visualization of CO1 gene amplikon, performed by electrophoresis technique. Sequencing, conducted through the First BASE Singapore sequencing service. The results show that there is a variation of local pigs CO1 gene in North Sulawesi. Variations are also found in the amino acid sequence encoded by the GH gene. Knowledge of the characteristics of local pig gh gene, the basics of selection of local pigs superior to North Sulawesi. Key words : growth hormone gen, local pigs, Sulawesi Utara Abstrak Gen growth hormone meregulasi reproduksi dan pertumbuhan pada mamalia. Telah dilaksanakan penelitian yang bertujuan untuk mendapatkan karakteristik gen GH pada babi lokal di Sulawesi Utara. Sampel babi diperoleh dari peternak tradisonal dari empat kabupaten di Sulawesi Utara. Ekstraksi dan purifikasi DNA menggunakan jaringan hipofisis babi. Amplifikasi gen GH menggunakan metode PCR. Visualisasi amplikon gen CO1 dilakukan dengan teknik elektroforesis. Sekuensing dilakukan melalui jasa layanan sekuensing First BASE Singapura. Hasil penelitian menunjukkan bahwa terdapat variasi gen CO1 babi lokal di Sulawesi Utara. Variasi juga ditemukan pada urutan asam amino yang dikode oleh gen GH. Diketahuinya karakteristik gen GH babi lokal, menjadi dasar seleksi babi lokal unggul Sulawesi Utara.

Optimizing the use of the docks in Ocean Fishing Port Bitung, North Sulawesi, Indonesia

2016 ◽  
Vol 4 (1) ◽  
pp. 17
Author(s):  
Iwan Hakim ◽  
K.W.A. Masengi ◽  
Alfret Luasunaung
Keyword(s):  
Good Condition ◽  
Reclaimed Land ◽  
North Sulawesi ◽  
Literature Reviews ◽  
Operational Criteria ◽  
Descriptive Method ◽  
Bahasa Indonesia

Title (Bahasa Indonesia): Pengoptimalan penggunaan dermaga di Pelabuhan Perikanan Samudera Bitung, Sulawesi Utara, Indonesia. This study was aimed to determine the basic and functional facilities conditions in the ocean fishing port of Bitung and analyze the level of utilization of basic and functional facilities. This research used a descriptive method, in which data were collected through observation, interviews, documentation, and literature reviews. Results indicated that the basic facilities, such as port, reclaimed land, docks 1 and 2, and the complex, were in good condition. The functional facilities were also good, so that the the fishing port of Bitung has met the requirements of technical and operational criteria for fisheries activities. Tujuan penelitian ini adalah untuk mengetahui kondisi fasilitas dasar dan fungsional yang ada di Pelabuhan Perikanan Samudera (PPS) Bitung dan menganalisis tingkat pemanfaatan fasilitas dasar dan fungsional yang ada di PPS Bitung. Penelitian ini menggunakan metode deskriptif di mana data dikumpulkan melalui observasi, wawancara, dokumentasi, dan studi pustaka. Dari hasil penelitian ini menunjukkan, bahwa fasilitas pokok yang terdiri dari lahan pelabuhan, lahan reklamasi, dermaga 1 dan 2, serta jalan komplek dalam kondisi baik dan fasilitas fungsional di PPS Bitung juga dalam kondisi baik, sehingga PPS Bitung sudah sesuai dengan kriteria teknis dan operasional dalam kegiatan perikanan.

scholarly journals Religious and Cultural Harmonies in the Art of Masamper

2016 ◽  
Vol 1 (2) ◽  
pp. 259
Author(s):  
Abu Muslim
Keyword(s):  
Religious Studies ◽  
Interpretive Research ◽  
Cultural Engagement ◽  
The Public ◽  
Cultural Arts ◽  
North Sulawesi ◽  
Local Tradition ◽  
Qualitative Descriptive ◽  
Depth Interviews ◽  
Descriptive Method

<p><em>This paper tries to track a potential religious harmony through a local tradition namely the art of Masamper in Sangir community. This study uses a qualitative- descriptive method to reveal the religious aspect in Art Masamper by first doing a study observations of implementation art in society, to further explore the values of divinity contained in Masamper process, to keep watching the dialectic of art and religion that were rolling.</em> <em>As one of the cultural-religious studies, this study is not an experimental research seeking a rule, but an interpretive research that searches for meaning.</em> <em>So that any meaning contained in the overall aspect of Masamper (processes, and religious dialectic of culture) becomes the point of analysis obtained from the observation, in-depth interviews, and a review of literatures related to discourse analysis.</em> <em>The results showed that Masamper as one of the remains of the ancestral cultural arts in Sangir society, other than as a means of proselytism, it also becomes one of the adhesives socio-religious harmony in North Sulawesi. Masamper can dilute the differences in beliefs into a cultural engagement through art performances which invites the public to appreciate each other in the name of culture and mutual respect in the practice of religious beliefs</em><em>.</em><em></em></p>

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