Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes

The family Channichthyidae or “icefishes” (suborder Notothenioidei) represents the only vertebrates lacking hemoglobin (Hb) as adults. Several icefish species also do not express cardiac myoglobin (Mb). We address how levels of proteins involved in iron (Fe) processing (transport, sequestration, and export) vary among white- and red-blooded notothenioids, and whether absence of Hb and/or Mb in channichthyids is accompanied by expansion of contents of Fe-binding proteins to protect against unchaperoned Fe. Levels of transferrin (Tf), ferritin (Ft), ceruloplasmin (Cp), and non-heme Fe were quantified in plasma, serum, and/or nonhematopoietic tissues (cardiac ventricle, skeletal muscle, and liver) from species of white-blooded ( Chaenocephalus aceratus, Champsocephalus gunnari, Chionodraco rastrospinosus, Pseudochaenichthys georgianus) (the first two species not expressing Mb) and red-blooded ( Notothenia coriiceps, Gobionotothen gibberifrons) notothenioids. We also measured levels of ascorbate (Asc), a mediator of Fe uptake. While plasma concentrations of Tf and tissue levels of Asc are similar among species, concentrations of plasma Asc are lower in white-blooded species. Concentrations of Ft and non-heme Fe and activities of Cp are also generally reduced in icefishes compared with red-blooded notothenioids. The presence of cardiac Mb in some icefish species does not appear to influence levels of proteins involved in Fe processing. To address further the question of Fe sequestration within a physiological context, we account for well-characterized differences in blood volume and heart mass among white- and red-blooded notothenioids. We report that total contents of plasma Tf are greater, while ventricle non-heme Fe is at least at parity in white- vs. red-blooded species.

Preliminary report on fish diversity at the Prince Gustav Channel, the northern part of the James Ross Island, Antarctica

Notothenioid fish were collected in the coastal part of the James Ross Island (Antarctica) mainly during Austral summer in 2012. In this paper, we report the list of species records and their basic biometric measurements as a reaction to missing ichthyofaunal investigation of this particular area. Collected fishes belong to the families Nototheniidae (Trematomus bernacchii Boulenger, 1902; T. eulepidotus Regan, 1914; T. newnesi Boulenger, 1902; Gobionotothen gibberifrons (Lönnberg, 1905); Notothenia coriiceps Richardson, 1844) and Bathydraconidae (Gymnodraco acuticeps Boulenger, 1902).

The diet of post-breeding Antarctic shagsPhalacrocorax bransfieldensisat the Danco Coast, Antarctic Peninsula

The diet of post-breeding Antarctic shagsPhalacrocorax bransfieldensiswas investigated at four colonies at the Danco Coast, Antarctic Peninsula, by the analysis of 399 pellets (regurgitated casts) collected during February and March 1998 and 2000. Overall, demersal-benthic fish were the most frequent and important prey at all the colonies sampled, followed by octopods and gastropods. Amongst the fish,Notothenia coriicepsandGobionotothen gibberifronswere the main prey in all of the sampling sites in both seasons. The composition of the diet of post-breeding shags differed from that observed in the previous breeding season. Post-breeders preyed on the same fish species consumed by breeders, although in different proportions and on larger specimens. The information provided here differs from that reported for post-breeding individuals belonging to other shag species and also for post-breeding Antarctic shags. Our results, as well as the differences with previous studies, are discussed in relation to differences in prey availability among localities and to the use of alternative foraging grounds at the end of the breeding period.

Photoperiod and feeding behavior of the Antarctic fish Notothenia rossii (Perciformes: Nototheniidae) and functional morphology of chemical and visual sensory structures used in foraging

The influence of photoperiod on the ability of Notothenia rossii Richardson, 1844 to detect prey was studied experimentally. In 12L/12D there was the highest number of individuals stimulated visually and chemically while in 24D chemical perception was the same as that observed in 12L/12D, the latter being lower than in 24L. The ultrastructure of chemo- and photo-sensory structures, involved in the detection of food, was studied. The photoreceptor cells of the retina are single and double cones, and rods. Taste buds were concentrated in the central part of upper and lower lips. Their shape is even, but the size is variable. The olfactory rosette has a sensory and a non-sensory epithelium, being rich in different mucosubstances. The conclusion was that in different photoperiods there is a difference in the response to visual and chemical stimulation for prey detection and that all sensory systems are more stimulated in periods of more luminosity, mainly when there is an alternation between light and dark periods. Sensory organs are complex when compared to some other Antarctic fish such as Gobionotothen gibberifrons Lönnberg, 1905 and Ophthalmolycus amberensis Tomo, Marshoff & Torno, 1977 but similar to those of N. coriiceps Rchardson, 1844.

Short Note: A method for sexing the chicks of Antarctic shags

Several studies have been carried out during the last fifteen years on reproduction, population dynamics and diet of the Antarctic shag, Phalacrocorax bransfieldensis Murphy, at different localities in the South Shetland Islands (reviewed in Casaux & Barrera-Oro 2006). In both the colonies studied and in other colonies counted we observed that the number of breeding pairs was steadily decreasing (Casaux & Barrera-Oro 2006). Casaux & Baroni (2002) had earlier suggested that such a decreasing trend might be related, at least partially, to a marked decrease in the inshore populations of two fish prey species, the marbled notothen Notothenia rossii Richardson and the humphead notothen Gobionotothen gibberifrons Lönnberg (Barrera-Oro et al. 2000), which had been studied over a period of 19 years in coastal waters of the South Shetland Islands. Exactly how a reduction in prey availability affects the shag populations (e.g. migration of breeders to other colonies in the area or to new breeding areas, a decrease in the rate of recruitment, an increase in adult mortality, variation in the age at first breeding, etc) is not clear. To investigate this, we started a banding programme at Nelson Island, South Shetland Islands. We postulated that the processes might operate with different intensities on individuals of different sexes, so all individuals in each population studied needed to be sexed. This posed problems for chicks which have monomorphic plumage and no differences in vocalisations (Casaux & Baroni 2000), so that the normal methods for sexing in the field would not work. As most of the external morphological characters in the chicks of Antarctic shags have stabilized by 45–50 days old (Casaux 1998), Casaux & Baroni (2000) had suggested that the use of discriminant functions originally developed for adults could be an appropriate method to sex chicks more than 50 days old.

Fish in the diet of the Antarctic shag at four colonies on the Danco Coast, Antarctic Peninsula

The diet of breeding Antarctic shags (Phalacrocorax bransfieldensis) was investigated at four colonies on the Danco Coast, Antarctic Peninsula, by the analysis of 616 pellets (regurgitated casts) collected from December 1997 to February 1998. Overall, demersal-benthic fish were the most frequent and important prey at all the colonies, followed by octopods and gastropods. Amongst fish, Notothenia coriiceps was the main prey in all of the sampling sites, followed in similar importance by Gobionotothen gibberifrons at Cape Herschel, Primavera Island and Midas Island and in less importance by Harpagifer antarcticus at Py Point. There were marked differences among colonies in the size of the fish consumed. The largest and the smallest specimens were eaten by shags from Midas Island and Py Point respectively. This was mainly influenced by the number of specimens of the smallest fish species, H. antarcticus, consumed at Py Point. The differences in the diet composition may be related to the different foraging areas used by the shags. Results from this study differ from previous studies around the Antarctic Peninsula. The shags at the Danco Coast preyed markedly more intensively than those at the South Shetland Islands on G. gibberifrons. This finding reflects the low abundance of this fish species in inshore waters (< 100 m depth) at the South Shetland Islands and supports the use of the Antarctic shags to monitor trends in local populations of coastal fish species.

Interannual variability in the early growth rate and size of the Antarctic fish Gobionotothen gibberifrons (Lönnberg)

Gobionotothen gibberifrons <1 year old, of the 1977, 1980, 1981, 1985, 1986, 1990 and 1995 cohorts, were sampled using nets, in various periods during summer (December to February) at South Georgia. Growth in standard length (Ls) was estimated using the exponential model. Among the seven cohorts, average growth rate varied between 0.33–2.1% Lsd−1, and predicted Ls for mid-January varied between 21.6–29.1 mm. Average growth rate was inversely related to mean date of sampling but was not significantly related to mean Ls. Mean sea surface temperatures were available for the 1981–95 cohorts. Average growth rate and predicted Ls for mid-January were both negatively conelated with mean December–January sea temperature, but were not significantly related to mean weekly sea surface temperature during the sampling periods. Greater average growth rate and greater Ls in cooler summers may be partly due to a large-scale pattern of environmental variability, indicated by sea temperature, that governs the timing and magnitude of the production cycle and food availability.

Two distinct types of fatty acid-binding protein are expressed in heart ventricle of Antarctic teleost fishes

This report provides the first evidence for the existence of two distinct types of fatty acid-binding protein (FABP) in cardiac tissue of vertebrates. Four species of Antarctic teleost fish (Chaenocephalus aceratus, Cryodraco antarcticus, Gobionotothen gibberifrons and Notothenia coriiceps) exhibited two FABP mRNAs of 1.0 kb and 0.8 kb, which we have termed Hh-FABP and Had-FABP (isolated from eart tissue, with similarity to mammalian eart-type FABP or mammalian ipose-type FABP respectively). These FABP types appear to be products of distinct genes. Both FABP transcripts were abundant in cardiac and aerobic pectoral muscle. However, relative abundance of the two types varied distinctly among other tissues such as kidney, brain, spleen and white muscle. Neither FABP type was expressed in liver or intestine. The coding regions of Hh-FABP and Had-FABP cDNAs from the same species are only ~ 60% identical with one another. However, homologues of each FABP species, which exhibit > 98% identity to their respective types, were isolated from three other Antarctic teleosts. Phylogenetic analysis of aligned amino-acid sequences places Hh-FABP with other vertebrate heart-type FABPs, and Had with adipose/cutaneous FABPs. Expression of two distinct FABPs in cardiac tissue of Antarctic teleosts may be related to their ability to both utilize fatty acid as the primary metabolic fuel and to store lipid intracellularly.

The summer diet of demersal fish at the South Shetland Islands

The stomach contents of demersal fish in late January 1982 were analysed. Samples were taken at 100, 300 and 500 m depth south of Elephant Island, Bransfield Strait and north of Livingston Island, and at 800 m to the east of Smith Island. Fifty four taxa of fish belonging to 11 families were collected. The diets of 2101 fish representing 38 taxa were examined. These were classified into three categories, fish feeders, krill feeders and benthos feeders. Fish prey species fed on krill and/or benthos. Krill was a major dietary component for 32 (84.2%) out of 38 taxa. Gobionotothen gibberifrons was distributed at all 10 stations (100–800 m in depth) and its diet comprised krill and benthos. The present findings verify the importance of krill in the Antarctic marine ecosystem and indicate that krill is consumed by benthic fish at greater depths than previously reported.