COVID-19 Serology Control Panel Using the Dried-Tube Specimen Method

The dried-tube specimen (DTS) procedure was used to develop the COVID-19 serology control panel (CSCP). The DTS offers the benefit of shipping materials without a cold chain, allowing for greater access without deterioration of material integrity. Samples in the panel were sourced from COVID-19 convalescent persons from March to May 2020. The immunoglobulin subtypes (total Ig, IgM, and IgG) and their respective reactivity to severe acute respiratory syndrome coronavirus 2 nucleocapsid, spike, and receptor-binding domain antigens of the samples were delineated and compared with the WHO International Standard to elucidate the exact binding antibody units of each CSCP sample and ensure the CSCP provides adequate reactivity for different types of serological test platforms. We distribute the CSCP as a kit with five coded tubes to laboratories around the world to be used to compare test kits for external quality assurance, for harmonizing laboratory testing, and for use as training materials for laboratory workers.

Biomechanical Exposure to Upper Extremity Musculoskeletal Disorder Risk Factors in Hospital Laboratories

Exposure to ergonomic risk factors has been reported for laboratory workers over decades. However, these exposures are not well characterized with respect to the type of laboratory or work organization. This study compared biomechanical exposure to upper extremity (UE) postures and hand activity levels (HALs) in general hospital laboratories by job, work, and laboratory type. The study used observational data gathered using a revised version of the Posture, Activity, Tools, and Handling (PATH) method, generating frequencies of categorized exposures. Eighteen workers were observed in 11 job titles (seven laboratories) in a single hospital by two investigators over a 7 month period. A taxonomy was constructed to categorize the extent to which the laboratory operations were automated. Overall, there were markedly high exposures to postural strain for the distal UE, especially wrist/forearm deviation (73% of observations), gross grasp (71%), and pinch grip (49%). For the HAL categories, 61% of the observations were in the moderate range (3.3–<6.7). Shoulders and elbows tended to remain in the neutral postural range. Posture frequencies were similar among the job categories studied and laboratory types. HAL was higher when the hand was in a pinch grip. Manual operations represented a higher proportion of work time than semi-automated or automated operations. Biomechanical exposure can be documented more extensively and diversely when using the revised PATH approach along with the taxonomy, with respect to exposure variables, such as the type of job, work, or organization in the industry including the hospital laboratories.

Assessing usability of QIAreach QuantiFERON® platform in a high Tuberculosis prevalence low-resource setting

BackgroundWe aimed to assess the usability of QIAreach QuantiFERON® platform in a high Tuberculosis prevalence low-resource setting. Assay usability was assessed across 6 laboratories in Zambia.MethodsUsability of QIAreach QuantiFERON and training needs for assay implementation were assessed across three domains: effectiveness, efficiency, and user satisfaction. Nine laboratory workers participated in the study. For each task, metrics on success (pass, fail, pass with hints), total task time, and ease of use rating (five-point Likert scale) were collected.ResultsOverall tasks completion rate was from 89–100%. 1/9 participants, could not understand software instructions. Average time from set up to results ranged from 22 to 40 min. Users with experience performing the QFT-plus assay completed the test faster than users without experience, 26 min versus 35 min. Two participants had difficulty loading the sample on QIAreach eStick. Two participants could not adjust the pipette to the required volume of 150 µL. Two participants did not mix the test sample properly. One participant transferred the sample buffer twice and added insufficient plasma to the sample processing tube. Two participants added the test sample to eStick sample port many times. One participant added wrong information in the software. User satisfaction ranged from 2 to 5.ConclusionThe QIAreach QFT assay is suitable to be implemented in remote areas with limited infrastructure. Further studies are needed to establish assay's performances as well as the feasibility of introducing this new assay at larger scale to improve TB control in regions with limited infrastructure.

Corrigendum: Brucellosis – Laboratory workers’ nightmare come true: A case study

No abstract available.

Use of The WISN Method to Assess the Health Workforce Requirements for the High-volume Clinical Biochemical Laboratories

Background: The clinical laboratory services, as an essential part of health care, require appropriate staff capacity to assure satisfaction and improve outcomes for both patients and clinical staff. This study aimed to apply the Workload Indicators of Staffing Need (WISN) method for estimating required laboratory staff requirements for the high-volume clinical biochemical laboratories. Methods: In 2019, we applied the WISN method in all 13 laboratories within the Center for Medical Biochemistry of the University Clinical Centre of Serbia (CMB UCCS). A review of annual routinely collected statistics, laboratory processes observations, and structured interviews with lab staff helped identify their health service and additional activities and duration of these activities. The study outcomes were WISN based staff requirements, WISN ratio and difference, and a recommendation on the new staffing standards for two priority laboratory workers (medical biochemists and medical laboratory technicians).Results: Medical biochemists' and laboratory technicians' annual available working time in 2019 was 1508 and 1347 working hours, respectively, for the workload of 1,848,889 samples. In general, the staff has four health service, eight support, and 15 additional individual activities. Health service activities per sample can take from 1.2 to 12.6 min. Medical biochemists and medical laboratory technicians spend almost 70% and more than 80% of their available working time, undertaking health service activities. The WISN method revealed laboratory workforce shortages in the CMB (i.e., current 40 medical biochemists and 180 medical laboratory technicians as opposed to required 48 medical biochemists and 206 medical laboratory technicians). Workforce maldistribution regarding the laboratory workload contributes to a moderate-high workload pressure of medical biochemists in five and medical laboratory technicians in nine organizational units.Conclusions: The WISN method showed mainly a laboratory workforce shortages and workload pressure in the CMB UCCS. WISN is a simple, easy-to-use method that can help decision-makers and policymakers prioritize the recruitment and equitable allocation of laboratory workers, optimize their utilization, and develop normative guidelines in the field of clinical laboratory diagnostics. WISN estimates require periodic reviews.

Analysis of Factors Affecting Waiting Time for Results of Clinical Laboratory Examinations at Medan Haji General Hospital

The purpose of this study was to analyze the factors that affect the waiting time for clinical laboratory examinations at RSU Haji Medan in 2021. This study is a mixed method. Using a total sampling of 17 people, namely all laboratory workers, the main informants in this study were clinical pathology doctors, room coordinators, clinical laboratory analysts at Haji Medan General Hospital (RSU) totaling 3 people and patients totaling 3 people. 1 additional informant, namely the Management section at RSU Haji Medan. The quantitative results are that there is a relationship between the qualifications of laboratory personnel having a value of p = 0.000, at the facility p = 0.044, there is no relationship between the transportation of specimens, the value of p = 0.099, there is a relationship between pre-analytical, analytical and pre-analytical problems. post analytic p value = 0.022, there is no electrical stability relationship p value = 0.235. Based on the results of research according to information and informants about the qualifications of officers running smoothly. have to send the sample to a private laboratory. Based on these results, it was concluded that the waiting time for laboratory results at RSU Haji has reached the standard that is in accordance with the minimum service standards at the Haji General Hospital in Medan City.

Hygienic Assessment of Risks of Thermal Balance Disruption in Medical Laboratory Workers Using Personal Protective Equipment for Biohazards

Introduction: Specifics of activities of medical personnel in different types of laboratories can potentially create working conditions that violate occupational safety and health regulations. Objective: The study aimed to assess health risks of thermal balance disruption in PCR laboratory staff wearing personal protective equipment for biohazards in the context of the COVID-19 pandemic. Materials and methods: The study was conducted in winter 2020–2021 in PCR laboratories of the Volgograd Region. It included measurements of laboratory microclimate parameters, such as relative humidity, air temperature and velocity, used to estimate the heat load index. An observation cohort of 31 female medical laboratory workers aged 32.48 ± 1.45 years with a three to nine months experience of work in the PCR laboratory was formed to monitor the thermal state at the start and end of the work shift by measuring skin temperature at five points, sublingual temperature, and heat sensation. The results of measurements were used to estimate the mean skin temperature and heart rate while the dynamics of work ability was assessed by the results of contact tremorometry. Results: We established a significant increase in all measured values. The mean skin temperature at the end of the shift reached 33.85 ± 0.24 °C, thus exceeding the upper limit of the permissible value. The heart rate and heat sensation parameters approached the upper values of the maximum permissible thermal state of a person. Contact tremorometry results indicated a decrease in the performance by the end of the work shift. The findings gave evidence of tension of thermoregulatory reactions and the risk of thermal balance disruption posed by the use of a specific type of personal protective equipment in the PCR laboratory. Conclusion: The research results provide strong support for the conclusion that the use of PPE for biohazards poses a risk of thermal balance disruption in medical laboratory personnel. The severity of stress of thermoregulatory reactions depends on technical and design characteristics of the PPE used. The necessity of a physiological and hygienic substantiation of acceptable duration of work of medical workers wearing various types of PPE for biohazards in PCR laboratories justifies the importance of further studies.

Serological Evidence of Human Infection with Coxiella burnetii after Occupational Exposure to Aborting Cattle

Cattle are broadly deemed a source of Coxiella burnetii; however, evidence reinforcing their role in human infection is scarce. Most published human Q fever outbreaks relate to exposure to small ruminants, notably goats. Anti-phase II C. burnetii IgG and IgM were measured by indirect fluorescent antibody tests in 27 farm and veterinary diagnostic laboratory workers to ascertain whether occupational exposure to cattle aborting due to C. burnetii was the probable source of exposure. Four serological profiles were identified on the basis of anti-phase II IgG and IgM titres. Profile 1, characterised by high IgM levels and concurrent, lower IgG titres (3/27; 11.1%); Profile 2, with both isotypes with IgG titres higher than IgM (2/27; 7.4%); Profile 3 with only IgG phase II (5/27; 18.5%); and Profile 4, in which neither IgM nor IgG were detected (17/27; 63.0%). Profiles 1 and 2 are suggestive of recent C. burnetii exposure, most likely 2.5–4.5 months before testing and, hence, during the window of exposure to the bovine abortions. Profile 3 suggested C. burnetii exposure that most likely predated the window of exposure to aborting cattle, while Profile 4 represented seronegative individuals and, hence, likely uninfected. This study formally linked human Q fever to exposure to C. burnetii infected cattle as a specific occupational hazard for farm and laboratory workers handling bovine aborted material.

Fighting COVID-19: The Medical Laboratory Involvement

The coronavirus disease-19 (COVID-19) virus has infected many people across the globe. The health system particularly medical laboratory has been overwhelmed by the pandemic, and many health professionals including medical laboratory professionals have lost their lives during the fight against the virus. Medical laboratory science is the bedrock of medical practice and the role of medical laboratory science in containing the COVID-19 pandemic cannot be overemphasized as they are also behind the testing of clinical specimens from infected and any recovered patients. As disease detectives, Medical laboratory scientists and other medical laboratory professionals’ role in the fight against the COVID-19 pandemic include; diagnosis, monitoring, development of vaccines, testing protocols, testing kits, offering advice to the guide government policy on containment of the virus.: Various methods and techniques such as virological cell culture, genomic sequencing, amplification, polymerase chain reaction (PCR) /gene Xpert systems, immunological testing, biosensors and rapid diagnostic techniques (RDTs) have been employed towards discovery, testing and epidemiology since the onset of COVID-19. The medical laboratory workers and other health workers are so visible at the COVID-19 frontline and are being recognized and applauded for the role played in the recovery of patients affected with the virus. The medical laboratory component is very germane in the COVID-19 vaccine research and vaccination so as to provide pre- and post-vaccination laboratory data.