Characterization and identification of compounds in neem (Azadirachta indica) seed oil

The seed oil of Azadrichta indica was obtained by Soxhlet extraction method. The maximum oil yield using petroleum ether was found 18.4% at a temperature of 70oC and a minimum yield of 13.5% at 30oC while at the same operating condition ethyl acetate and methanol resulted a maximum yield of 17.0% and 16.5% and a minimum of 12.0% and 13.0% respectively. So petroleum ether is a good solvent to afford high yield in this work. The oil was analyzed by GC-MS and result in the identification of the compounds (palmitoleic acid methyl ester, palmitic acid methyl ester, linoleic acid methyl ester, oleic acid methyl ester, stearic acid methyl ester, etc.) was obtained. In the present study various physical and chemical characteristics have been also studied. The results also indicated that the oil extracted from neem seed is the most acidic and non edible.

Micropropagation and Screening of Phytocompounds Present among in vitro Raised and Wild Plants of Rauvolfia serpentine

Rauvolfia serpentina, commonly known as Sarpgandha, is a plant well-known for being utilized for several medicinal purposes. Unrestricted collection from natural stands and overexploitation for medicinal and traditional purposes have rendered it endangered, hence there is necessary requirement for the development of cultivation protocols for mass propagation and sustained utilization of the plant. In the present study, in vitro culture of the apical parts of R. serpentina on MS (Murashige and Skoog) medium enriched with NAA resulted in development of callus, whereas multiple shoot regeneration along with callus development was achieved on medium combination MS + NAA + Kn and MS + NAA + BAP. MS + 4µM NAA + 4µM BAP was found to be most optimum media composition for regeneration of shoots and callus. Among different media combinations utilized for in vitro rooting, a maximum of 82.6 % explants developed in vitro roots on ½ MS + 12µM NAA. Gas chromatography-mass spectrometry (GC-MS) analysis of methanolic extract prepared from leaves of wild and micropropagated plants of R. serpentina revealed the presence of 38 and 48 phytocompounds, respectively. 9, 12-Octadecadienoic acid, Methyl linoleate, Methyl stearate, Hexadecanoic acid, methyl ester, Linoleic acid, Ergost-4, 7, and 22-trien-3.alpha.-ol were some of the major compounds found to be present in the leaves of wild plants, and Linoleic acid, methyl ester, Cis-Linoleic Acid Methyl Ester, Methyl elaidate, hexadecanoic acid, and methyl ester were major compounds found to be present in in vitro raised plants. Many of the compounds detected have been known to possess 1 or more biological or pharmacological activities.

ANALISIS KOMPONEN ASAM LEMAK DAN MINERAL (Ca, Mg, Fe, Zn) IKAN KAKAP PUTIH (Lates calcalifer)

ABSTRACT The analysis of fatty acid component and mineral composition (Ca, Mg, Fe, Zn) of white snapper fish (Lates calclifer) had been done. Flesh of white snapper fish (Lates calclifer) was extracted with petroleum benzene and obtained 6.06% of oil. Oil fish was transesterified by using BF3 – methanol 20%. The result of transesterification was analysed by using and GC-MC obtained nine fatty acid component, those were myristic acid methyl ester 5.94%; pentadecanoic acid methyl ester 1,58%; palmitoleic acid methyl ester 8.73; palmi acid methyl ester 17.69; Margarate acid methyl ester 1.96%; linoleic acid methyl ester 1.56%; oleic acid methyl ester 15.83%; streaic acid methyl ester 10.41%; aracidonic acid methyl ester 5.26%. the result of minerals composition analysis used atom absorption spectrophotometer (ASS) showed that the content of calcium (Ca), Magnesium (Mg), ferrum (Fe), zinc (Zn), were 51,1 mg/kg; 574 mg/kg; 65 mg/kg; and 10,8 mg/kg, respectively. Oil of white snapper fish (Lates calcalifer) contains esensial fatty acid that is benefit to humans body. In the another hand, it also contains mineral content which is enough that it is goof to be consumend by the people.

Influence of Oryzanols Concentrate on the Oxidation of Methyl Ester Linoleic Acid and Study of Their Oxidation Products

Oryzanols are frequently found in rice bran oil but almost completely removed in the neutralization step when the oil is chemically refined. In this way, oryzanols can be recovered from the soapstocks to generate a concentrate. Thereby, they could be used as antioxidants in lipids for specific purposes. In the present work the antioxidant power of oryzanols concentrate (33% purity) was studied together with pure oryzanols and butylhydroxytoulene (BHT). Methyl esters were prepared from regular sunflower oil without antioxidants to which the antioxidants before mentioned were added in an effective concentration of 3x10-3 M. The samples were oxidized in a heating block with oxygen flow and the hydroperoxides of linoleic acid methyl ester were analyzed. It was observed that all antioxidants were able to protect the sunflower oil methyl esters from oxidation with respect to methyl esters without antioxidants. Oryzanols presented a notoriously lower protection ability compared to BHT. However, the formation of the linoleic acid methyl ester hydroperoxides and their proportion, for the same oxidation stage, did not show differences between the antioxidants used. Therefore, the oxidation kinetics were similar between the different antioxidants studied.

New oxaphenalene derivative from marine-derived Streptomyces griseorubens sp. ASMR4

During our search for novel bioactive compounds from extremophilic actinomycetes, the new Streptomyces griseorubens sp. ASMR4 was isolated from a soft coral collected in the Red Sea at the Hurghada coast, Egypt, and characterized taxonomically. It was fermented on large scale using a modified solid rice medium as the first example for actinomycetes so far. Work-up and purification of the strain extract using different chromatographic techniques afforded the new oxaphenalene derivative, 8-hydroxy-2-(2-hydroxypropyl)-7-acetyl-1-oxaphenalene (1a), together with seven known metabolites: ferulic acid (2), glycerol linoleate, linoleic acid methyl ester, (3R,4R)-3,4-dihydroxy-3-methylpentan-2-one/(3S,4R)-3,4-dihydroxy-3-methylpentan-2-one, anthranilic acid, phenylacetic acid, and benzoic acid. The chemical structure of the new compound (1a) was confirmed by extensive 1D and 2D NMR spectroscopy, high-resolution electron impact mass measurements, and by comparison with literature data. The antimicrobial activity of the strain extract and compounds 1a and 2 were studied using a panel of pathogenic microorganisms. The in vitro cytotoxicity of the bacterial extract was studied against the human cervix carcinoma cell line (KB-3-1) and its multi-drug-resistant subclone (KB-V1).

Repellent and Insecticidal Activities of Melia azedarach L. against Cotton Leafworm, Spodoptera littoralis (Boisd.)

A crude acetone extract and oil of ripe fruits from Melia azedarach L. were evaluated against the 2nd and 4th instar larvae of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Both oil and extract exhibited highly significant growth inhibition at all concentrations tested, while the oil of M. azedarach recorded higher insecticidal activity against both instars than the crude extract. GC-MS analysis of the oil revealed the presence of linoleic acid methyl ester, oleic acid methyl ester, and free oleic acid as the main components in addition to hexadecanol, palmitic acid, methyl esters of stearic acid and myristic acid. Fatty acids and their esters were not only the main constituents of essential oil from the ripe fruits of M. azedarach, but also mainly responsible for the insecticidal and growth inhibition activity against S. littoralis