Analisis Kandungan Asam Lemak Mudah Terbang dengan Penambahan Buah Sirih dalam Ransum Ruminansia (In-Vitro)

Further socialization is needed regarding the importance of paying attention to the basic nutritional needs of cattle. The use of local plants that are often used by the community, such as betel fruit, is a worthy study to be explored. However, people still do not know the other benefits contained in betel fruit. In order to obtain optimal results, the study was carried out with in vitro experiments. Data processing was performed using factorial completely randomized design with three replications. The treatment factors were differentiated based on the length of the betel fruit immersion process in ice water and the length of storage time. The analysis showed that cold storage of grass with betel fruit was successful in increasing the Volattile Fatty Acid component (P> 0.01). The habits of the people of West Papua in utilizing local plants, especially those with tannin, have been scientifically proven to be useful. Cold storage of betel fruit with the addition of grass as ration for cattle has a very significant effect on the levels of iC4 (as iso butyrate), nC4 (as n butyrate), iC5 (as iso valerate), and nC5 (as n valerate). In addition, the Papuan people's habit of adding local plants, especially betel fruit as additional feed has been scientifically proven to be useful. Keywords: Betel Fruit, Ruminants, Grass, In Vitro, VFA

ANALISIS KOMPONEN ASAM LEMAK DAN MINERAL (Ca, Mg, Fe, Zn) IKAN KAKAP PUTIH (Lates calcalifer)

ABSTRACT The analysis of fatty acid component and mineral composition (Ca, Mg, Fe, Zn) of white snapper fish (Lates calclifer) had been done. Flesh of white snapper fish (Lates calclifer) was extracted with petroleum benzene and obtained 6.06% of oil. Oil fish was transesterified by using BF3 – methanol 20%. The result of transesterification was analysed by using and GC-MC obtained nine fatty acid component, those were myristic acid methyl ester 5.94%; pentadecanoic acid methyl ester 1,58%; palmitoleic acid methyl ester 8.73; palmi acid methyl ester 17.69; Margarate acid methyl ester 1.96%; linoleic acid methyl ester 1.56%; oleic acid methyl ester 15.83%; streaic acid methyl ester 10.41%; aracidonic acid methyl ester 5.26%. the result of minerals composition analysis used atom absorption spectrophotometer (ASS) showed that the content of calcium (Ca), Magnesium (Mg), ferrum (Fe), zinc (Zn), were 51,1 mg/kg; 574 mg/kg; 65 mg/kg; and 10,8 mg/kg, respectively. Oil of white snapper fish (Lates calcalifer) contains esensial fatty acid that is benefit to humans body. In the another hand, it also contains mineral content which is enough that it is goof to be consumend by the people.

Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria

Salipiger mucescens gen. nov., sp. nov. is a moderately halophilic, exopolysaccharide-producing, Gram-negative rod isolated from a hypersaline habitat in Murcia in south-eastern Spain. The bacterium is chemoheterotrophic and strictly aerobic (i.e. unable to grow under anaerobic conditions either by fermentation or by nitrate or fumarate respiration). It does not synthesize bacteriochlorophyll a. Catalase and phosphatase are positive. It does not produce acids from carbohydrates. It cannot grow with carbohydrates or amino acids as sole sources of carbon and energy. It grows best at 9–10 % w/v NaCl and requires the presence of Na+ but not Mg2+ or K+, although they do stimulate its growth somewhat when present. Its major fatty-acid component is 18 : 1ω7c (78·0 %). The predominant respiratory lipoquinone found in strain A3T is ubiquinone with ten isoprene units. The G+C content is 64·5 mol%. Phylogenetic analyses strongly indicate that this strain forms a distinct line within a clade containing the genus Roseivivax in the subclass α-Proteobacteria. The similarity value with Roseivivax halodurans and Roseivivax halotolerans is 94 %. In the light of the polyphasic evidence gathered in this study it is proposed that the isolate be classified as representing a new genus and species, Salipiger mucescens gen. nov., sp. nov. The proposed type strain is strain A3T (=CECT 5855T=LMG 22090T=DSM 16094T).

Partial hydrogenation of edible oils: Synthesis and verification of the nickel catalyst

Developed in the late 1800s as a butter substitute, margarine is made with vegetable oils. Margarine was created by a scientist from Provence (France) Hippolyte Mege-Mouries, in response to an offer by the Emperor Louis Napoleon III. To formulate his entry, Mege-Mouries used margaric acid, a fatty acid component isolated in 1813 by Michael Chevreul and named because of the lustrous pearly drops that reminded him of the Greek word for pearl - margarites.

Evidence and characterization of a gene cluster required for the production of viscosin, a lipopeptide biosurfactant, by a strain ofPseudomonas fluorescens

The genetic control of viscosin production was examined in a strain of Pseudomonas fluorescens (PfA7B) that causes broccoli head rot. Viscosin is a potent lipopeptide biosurfactant that enables the bacteria to come into intimate contact with the difficult-to-wet waxy heads of broccoli. Tn5 mutagenesis completely disrupted viscosin production as shown by HPLC analysis of the mutagenized cell lysates. The Vis–mutants retained their pectolytic capability and were able to decay potato slices. On broccoli, however, the Vis–mutants caused decay of wounded florets, but the decay failed to spread to adjacent nonwounded florets as had occurred with the wild-type PfA7B. Triparental matings of the Vis–mutants with their corresponding wild-type clones and the helper Escherichia coli HB101 carrying the mobilization plasmid pPK2013 resulted in three stable viscosin-producing transconjugants that caused typical decay of broccoli tissue. Linkage maps of clones and protein profiles showed that a 25-kb chromosomal DNA region of PfA7B affected the production of three high molecular mass proteins required for viscosin synthesis. These proteins, approximately 218, 215, and 137 kDa in size, likely compose a synthetase complex that assembles the nine amino acid peptide of viscosin and subsequently attaches this to the hydrophobic fatty acid component of the molecule. A probe made from this DNA region hybridized with DNA fragments of other phytopathogenic pseudomonads to varying degrees.Key words: virulence factor, head rot, broccoli.

The nature of Covalently bound fatty acids in wool fibres

The wool fibre contains a fatty acid component which can only be liberated from the fibre by treatment with alcoholic alkali solutions. The major fatty acid from this component has been isolated in quantity and purified. Using GC/MS and NMR, the fatty acid was identified as 18-methyleicosanoic acid. The results obtained from transesterification experiments suggest that the fatty acid is covalently bound to the fibre protein by an ester linkage.