The Development of a Technique for Sensitizing Cells of Various Origins for Biotechnological Purposes

The functional activity stimulation of cell cultures was tested in MDBK cell culture, photobacteria AliivibriofischeriandHalobacteriumhalobium. Theaim of the investigation was to increase the ”yield” of the cells using an environmentallysafe stimulant and membrane-tropic agent that isalso safe for the experimenter. Ultrasonicwaves were used.Experimental ultrasonic exposure varied within the following limits: time from 1 to 300 sec, SATA-intensity of 0.01–2.0 W/cm2, generation frequency of 0.88 or 2.64 MHz, standing or traveling wave. The modulation frequency range was within 0.1–150 Hz. The devices used were: UST-1-01F, UST-5 and UST1.02C. The modulating generators were G3–112 and CP–110.Stimulation of MDBK cell growth was initiated by US-intensity of 0.03–0.05 W/cm2 , with an exposure of 5–30 sec.Exposure to ultrasound with an intensity of 0.2–0.4 W/cm2 (for 3 min) had a stimulating effect on bioluminescence and was associated with an increase in the growth rate ofA. fischeri. The findings indicated that 0.4 W/cm2ultrasonic intensity and modulation frequencies from 0.25 to 0.7 Hz can stimulate the growth of archaea.It was revealed that the maximum proliferation index in all cases of stimulant application was noted in cultures with minimal initial proliferative activity in the control.The authors expect thatthese results on the possibilities of acoustic continuous and modulated waves can be applied for biotechnological purposes to develop a new biotechnological method. Keywords: cell culture, ultrasound, proliferation, stimulation

Pulse density modulation control-based multi-load handling three leg inverter for cooking applications

Purpose Induction heating applications aided by power electronic control have become very attractive in the recent past. For cooking applications, power electronics circuits are very suitable to feed power to multi loads with an appropriate control technique. The purpose of this paper is to develop a three leg inverter to feed power to three loads simultaneously and independently. Design/methodology/approach Pulse density modulation control technique is used to control the output power independently with constant switching frequency. Findings Multi-load handling converter with independent power control is achieved with reduced number of switching devices (two switches/per load) with simple control strategy. Originality/value The proposed system is simulated in MATLAB/Simulink, and the thermal analysis is carried out in COMSOL multi-physics software. The hardware realisation is performed for a 1 kW prototype with 20 kHz switching frequency and 10 kHz pulse density modulation frequency. PIC16F877A microcontroller is used to validate the experimental results for various values of control signals (DPDM). The simulation and experimental results are in good agreement and validates the developed system.

Theoretical Analysis of Coupled Modified Hindmarsh-rose Model Under Transcranial Magnetic-acoustic Electrical Stimulation

Transcranial magnetic-acoustic electrical stimulation (TMAES) is a new technology with ultrasonic waves and a static magnetic field to generate an electric current in nerve tissues to modulate neuronal firing activities. The existing neuron models only simulate a single neuron, and there are few studies on coupled neurons models about TMAES. Most of the neurons in the cerebral cortex are not isolated but are coupled to each other. It is necessary to study the information transmission of coupled neurons. The types of neuron coupled synapses include electrical synapse and chemical synapse. A neuron model without considering chemical synapses is not comprehensive. Here, we modified the Hindmarsh-Rose (HR) model to simulate the smallest nervous system—two neurons coupled electrical synapses and chemical synapses under TMAES. And the environmental variables describing the synaptic coupling between two neurons and the nonlinearity of the nervous system are also taken into account. The firing behavior of the nervous system can be modulated by changing the intensity or the modulation frequency. The results show that within a certain range of parameters, the discharge frequency of coupled neurons could be increased by altering the modulation frequency, and intensity of stimulation, modulating the excitability of neurons, reducing the response time of chemical postsynaptic neurons, and accelerating the information transferring. Moreover, the discharge frequency of neurons was selective to stimulus parameters. These results demonstrate the possible theoretical regulatory mechanism of the neurons' firing frequency characteristics by TMAES. The study establishes the foundation for large-scale neural network modeling and can be taken as the theoretical basis for TMAES experimental and clinical application.

Research on the Dual Modulation of All-Fiber Optic Current Sensor

Acousto-optic modulator (AOM) and electro-optical modulator (EOM) are applied to realize the all-fiber current sensor with a pulsed light source. The pulsed light is realized by amplitude modulation with AOM. The reflected interferometer current sensor is constructed by the mirror and phase modulation with EOM to improve the anti-interference ability. A correlation demodulation algorithm is applied for data processing. The influence of the modulation frequency and duty cycle of AOM on the optical system is determined by modeling and experiment. The duty cycle is the main factor affecting the normalized scale factor of the system. The modulation frequency mainly affects the output amplitude of the correlation demodulation and the system signal-to-noise ratio. The frequency multiplication factor links AOM and EOM, primarily affecting the ratio error. When the frequency multiplication factor is equal to the duty cycle of AOM and it is an integer multiple of 0.1, the ratio error of the system is less than 1.8% and the sensitivity and the resolution of AFOCS are 0.01063 mV/mA and 3 mA, respectively. The measurement range of AFOCS is from 11 mA to 196.62 A, which is excellent enough to meet the practical requirements for microcurrent measurement.

DETERMINATION OF THE ACOUSTO-OPTICAL DEFLECTOR PARAMETERS FOR A LASER-RADIATION ROCKET GUIDANCE SYSTEM

The work is devoted to the development of an acousto-optic deflector for a laser-beam guidance system (LLSN) of missiles. LLSN is used in semiautomatic portable missile systems to destroy hostile targets of various types. An analysis of the methods for constructing such systems has shown that the most promising devices with pulse-code modulation using semiconductor pulsed lasers. The article provides a diagram and describes the principle of operation of the LLSN with pulse-code modulation. A problematic issue in the implementation of such a system is the development of a device for deflecting a laser beam, through which the missile is guided to a target. Scanning mechanical devices that are currently in use have a complex design, significant dimensions and weight, and limited performance. The article proposes to use an acousto-optic deflector to deflect the laser beam within the information field of the guidance system, which is devoid of these disadvantages, since it replaces the mechanical scanning device with an electronic one. The purpose of the article is to determine the main parameters of the acousto-optical deflector. The article discusses the principle of operation of an acousto-optic deflector. It is noted that glasses based on germanium chalcogenides, in particular, glass with the composition Ge2.17As39.13S58.70, have especially low values of acoustic losses (α <1 dB / cm). The largest deflection angle of the laser beam will be observed with Bragg diffraction. Relationships are given that can be used to determine the main characteristics of the deflector: the angle of deflection of the laser beam, the modulation frequency of the acoustic wave, resolution, speed, and others. When using the above ratios for the typical parameters of the existing guidance system, the values of the indicated characteristics are calculated.

Soliton solutions for Hirota–Maxwell–Bloch system and its nonlocal form

This paper studies the Hirota–Maxwell–Bloch (H–MB) system and its nonlocal form. Based on the Darboux Transformations (DTs), for H–MB system, we present general double breathers, what is more, we take appropriate modulation frequency and position parameters to investigate the generative mechanism of rogue wave sequences and different periodic breather sequences. For nonlocal Hirota–Maxwell–Bloch (NH–MB) system, we discuss symmetry preserving and broken soliton solutions under zero background. Besides, we present nine combinations of dark and antidark soliton solutions under continuous waves background when PT-symmetry is broken.

Timescale of degradation-driven protein level fluctuation in the yeast Saccharomyces cerevisiae

Generating robust, predictable perturbations in cellular protein levels will advance our understanding of protein function and enable control of physiological outcomes in biotechnology applications. Previous studies have shown that controlling RNA transcription achieves perturbations in protein levels over a timescale of several hours. Here, we demonstrate the potential for harnessing the protein degradation machinery to achieve robust, rapid control of a specific protein level in the yeast Saccharomyces cerevisiae. Using a light-driven protein degradation machinery and red fluorescent proteins as reporters, we show that under constant transcriptional induction, repeated triangular fluctuations in protein levels can be generated by controlling the protein degradation rate. Consistent with previous results using transcriptional control, we observed a continuous decrease in the magnitude of fluctuations as the modulation frequency increased, indicating low-pass filtering of input perturbation. However, compared to hour-scale fluctuations observed using transcriptional control, modulating the protein degradation rate enabled five to ten minute-scale fluctuations. Our study demonstrates the potential for repeated control of protein levels by controlling protein degradation rate, at timescales much shorter than that achieved by transcriptional control.

Cortical Processing of Binaural Cues as Shown by EEG Responses to Random-Chord Stereograms

Spatial hearing facilitates the perceptual organization of complex soundscapes into accurate mental representations of sound sources in the environment. Yet, the role of binaural cues in auditory scene analysis (ASA) has received relatively little attention in recent neuroscientific studies employing novel, spectro-temporally complex stimuli. This may be because a stimulation paradigm that provides binaurally derived grouping cues of sufficient spectro-temporal complexity has not yet been established for neuroscientific ASA experiments. Random-chord stereograms (RCS) are a class of auditory stimuli that exploit spectro-temporal variations in the interaural envelope correlation of noise-like sounds with interaurally coherent fine structure; they evoke salient auditory percepts that emerge only under binaural listening. Here, our aim was to assess the usability of the RCS paradigm for indexing binaural processing in the human brain. To this end, we recorded EEG responses to RCS stimuli from 12 normal-hearing subjects. The stimuli consisted of an initial 3-s noise segment with interaurally uncorrelated envelopes, followed by another 3-s segment, where envelope correlation was modulated periodically according to the RCS paradigm. Modulations were applied either across the entire stimulus bandwidth (wideband stimuli) or in temporally shifting frequency bands (ripple stimulus). Event-related potentials and inter-trial phase coherence analyses of the EEG responses showed that the introduction of the 3- or 5-Hz wideband modulations produced a prominent change-onset complex and ongoing synchronized responses to the RCS modulations. In contrast, the ripple stimulus elicited a change-onset response but no response to ongoing RCS modulation. Frequency-domain analyses revealed increased spectral power at the fundamental frequency and the first harmonic of wideband RCS modulations. RCS stimulation yields robust EEG measures of binaurally driven auditory reorganization and has potential to provide a flexible stimulation paradigm suitable for isolating binaural effects in ASA experiments.