Water orientation and dynamics in the closed and open influenza B virus M2 proton channels

The influenza B M2 protein forms a water-filled tetrameric channel to conduct protons across the lipid membrane. To understand how channel water mediates proton transport, we have investigated the water orientation and dynamics using solid-state NMR spectroscopy and molecular dynamics (MD) simulations. 13C-detected water 1H NMR relaxation times indicate that water has faster rotational motion in the low-pH open channel than in the high-pH closed channel. Despite this faster dynamics, the open-channel water shows higher orientational order, as manifested by larger motionally-averaged 1H chemical shift anisotropies. MD simulations indicate that this order is induced by the cationic proton-selective histidine at low pH. Furthermore, the water network has fewer hydrogen-bonding bottlenecks in the open state than in the closed state. Thus, faster dynamics and higher orientational order of water molecules in the open channel establish the water network structure that is necessary for proton hopping.

Restoration of the disturbed natural hydrological regime of the fish-breeding channel Kazachy Yerik within the framework of fishery reclamation

Intensive economic use of small rivers and channel water bodies leads to their pollution and siltation. The anthropogenic impact on aquatic ecosystems negatively affects the state of the fish industry, which leads to a reduction in the spawning fund, deterioration of the conditions for reproduction and survival of juveniles. Violation of the existing conditions for the habitat and reproduction of aquatic organisms leads to a decrease in the productivity of water bodies and the loss of their fishery value. The inflow from the catchment area of solid and liquid runoff containing organic matter, oil products and other impurities of anthropogenic and technogenic origin leads to irreversible entry, siltation and shallowing of small rivers and channel water bodies. The restoration of the disturbed hydrological regime of water bodies by means of reclamation works contributes to the restoration of natural spawning grounds for commercial fish species, an increase in yield and productivity.

Filtration reliability and safety of earth-fill dam

The study of the reliability and safety of the constructed earth-fill dams and the comparison with their design and calculated data makes it possible to improve the structures and methods of the calculation substantiation of these structures. This work aims to study the filtration reliability and safety of the earth-fill dam of the Channel water reservoir of the Tuyamuyun hydroelectric complex (THC) on the Amu Darya River, which was put into exploitation in 1984. Field studies were carried out according to the traditional method using results of control and measuring equipment (CME) embedded in the body of the dam. The water levels of the upper and lower reaches, piezometers, and drainage water flow were measured. The maximum water levels upstream of 130.00 were observed in July-August and November 2017, and the minimum of 117.50 at the end of March. The water levels downstream depend on the value of the discharge through the hydrosystem. The maximum level downstream for 2017 was 112.55 m (01.06.2017) with a flow rate of 2000 m3/s. The minimum level downstream of 109.15 m was observed on November 29, 2017, when the discharge into the downstream through the hydroelectric complex was 260 m3/s. A tendency to an increase in the level of the bottom downstream was found. Filling and depletion graphs of the Channel water reservoir have been built, from which it is found that they reached 2.00 m/day, and 1.60 m/day, respectively. This is 4 and 1.6 times more than the standard 0.5 m/day and 1.0 m/day. Of the 53 piezometers, 34 are working conditions; the rest do not work, require flushing. Graphs of water level changes in piezometers show that they change with an average 15-20 day delay in the water level in the Channel water reservoir. In general, the natural depression curve is below the design one. The maximum filtration flow rate was 63.3 l/s at a water level in the upper pool of 129.00.

Studies on Tropics of Fish Along Upper Tungabhadra Channel, Ballari District, Karnataka

Gut analysis is the tool to understand the feeding patterns of fishes and is an important aspect of fisheries management. It also provides the basis for understanding trophic interactions in aquatic food webs and to investigate the most frequently consumed prey or to determine the relative importance of different food types to fish nutrition. In the present study the gut content analysis was performed in Garra, Gobi, Notopterus and Tilapia fishes collected from Tungabhadra upper irrigation channel at Ballari, Karnataka.Bacillariophyceae showed maximum number in all the four fish species. Over all it showed 40% followed by Detritus (30%), Chlorophyceae (17%), Cyanophyceae(7%) and Zooplankton (6%). Among fishes Garrashowed maximum food items (2272) followed by Glossogobiusgiuris(1538), Notopterusnotopterus (996) and Oreochromismossambicus (769). The relative abundance of food items in the guts also revealed the Garragotylastenorhynchus Oreochromismossambicus. The variation is due to availability of foodorganisms during the study period and anthropogenic influence on channel water.

The seasonal changes of the chemical composition of water from the Kola Nuclear Power Plant discharge channel.

In this paper, the change in the chemical composition of water in the discharge channel in the spring and autumnperiodswas studied. An increase in the concentration of iron, alkali metals and a decrease in alkaline earth and non-ferrous metals in the autumn period compared to the spring period was revealed. It can be explained by changes in the discharge channel water temperature and acidity and also the presence of living organisms.

Optimizing Parallel Pumping Station Operations in an Open-Channel Water Transfer System Using an Efficient Hybrid Algorithm

This article presents a methodology for optimizing the operation of parallel pumping stations in an open-channel water transfer system. A mathematical model was established for the minimum power with constraints on water level, flow rate and pump unit performance, and related factors. In the objective function, energy consumption of relevant equipment or facilities, such as main pump units, power transmission and transformation equipment, and auxiliary equipment, was considered comprehensively. The model was decomposed to two layers for solving. In the first layer, by using discharge distribution ratio as a variable, the flow rate and water level of the two water channels could be determined by employing the dichotomy approach (DA), and were calculated according to the principle of energy conservation, considering energy loss caused by hydraulic leakage and evaporation losses. In the second layer, the number of running pumps and the flow rate of a single pump were obtained by simulated annealing–particle swarm optimization (SA–PSO). The hybrid of the two algorithms is called the dichotomy approach–simulated annealing–particle swarm optimization (DA–SA–PSO). To verify the efficiency and validity of DA–SA–PSO, SA–PSO is also applied to determine discharge distribution ratio. The results indicate that the computation time using DA–SA–PSO is 1/30 of that using double-layer SA–PSO (dSA–PSO). Compared with the original plan, the optimal solution could result in power savings of 14–35%. Thus, the DA–SA–PSO is highly efficient for optimizing system operation in real time.