Performance of long-chain mid-chan diol based temperature and productivity proxies at test: a five-years sediment trap record from the Mauritanian upwelling

Long-chain mid-chain diol (shortly diol) based proxies obtain increasing interest to reconstruct past upper ocean temperature and productivity. Here we evaluate performance of the sea surface temperature proxies; long chain diol index (LDI), the diol saturation index (DSI) and the diol chain-length index (DCI), productivity/upwelling intensity proxies: the two diol indices DIR (Rampen et al., 2008) and DIW (Willmott et al., 2010) and the combined diol index (CDI), as well as the nutrient diol index (NDI) as proxy for phosphate and nitrate levels. This evaluation is based on comparison of the diols in sediment trap samples from the upwelling region off NW Africa collected at 1.28 km water depth with daily satellite derived sea surface temperatures (SSTSAT), subsurface temperatures, productivity, the plankton composition from the trap location, monthly PO43− and NO3− concentrations, wind speed and wind direction from the nearby Nouadhibou airport. The diol based SST reconstructions are also compared the long chain alkenones based UK’37 proxy reconstructions (SSTUK). At the trap site, most diol proxies lag wind speed (phase φ = 30 days) and can be related to upwelling. Correlation with the abundance of upwelling species and wind speed is best for the DCI, DSI and NDI whereas the DI and CDI perform comparatively poorly. The nutrient proxy NDI shows no significant correlation to monthly PO43− and NO3− concentrations in the upper waters and a negative correlation with wind-induced upwelling (r2 = 0.28, φ = 32 days) as well as the abundance of upwelling species (r2 = 0.38; Table 4). It is suggested that this proxy reflects upwelling intensity rather than upper ocean nutrient concentrations. At the trap site, SSTSAT lags wind speed forced upwelling by about 4 months (φ = 129 d). The LDI based SST (SSTLDI) correlate poorly (r2 = 0.17) to SSTSAT which we attribute to variability in 1,13 diol abundance unrelated to SST such as productivity. The SSTUK correlates best with SSTSAT (r2 = 0.60). Also amplitude and absolute values agree very well and the flux corrected SSTUK time series average equals the SSTSAT annual average.

Monitoring Chilecomadia valdiviana (Lepidoptera: Cossidae) Using Sex Pheromone-Baited Traps in Apple Orchards in Chile

Chilecomadia valdiviana (Philippi) (Lepidoptera: Cossidae) is a native xylophagous pest in apple orchards in Chile. A series of experiments evaluated the efficacy of trap type, sex pheromone (Z7,Z10-16:Ald) dose, and trap location in the apple tree canopy on trap catch of male adults. Bucket traps (6 L), with and without roof and cross vane spacers, together with bucket traps (20 L) without roof and spacers, showed higher catches among the four types of traps evaluated. In a second experiment, the UNI-trap and Delta trap showed higher catches than Multipher, wing, and bucket traps (6 L). Male catches were not affected by height when tested at 0, 1.5, and 3 m in the canopy. A 300 µg dose of Z7,Z10-16:Ald showed higher catch than the control treatment. This dose allowed monitoring of male flight of C. valdiviana for at least five weeks in apple orchards in Chile. Based on relative trap costs, we propose the use of 6 L bucket traps for male mass trapping, while Delta traps can be used for monitoring of male flight. We found that male flight of C. valdiviana occurred mainly from mid-August to late November, reaching its maximum in mid-September.

Prevalence of Salmonella enterica in flies on a Diversified Cattle and Fresh Produce Farm Across Two Growing Seasons

Flies are a vector for spreading foodborne pathogens pertinent to fresh produce, such as Shiga-toxigenic Escherichia coli and Salmonella; however, most studies focus on concentrated animal feeding operations, which do not reflect low-density animal farming practices that often adjoin fruit and vegetable acreage. In this study, we determined the prevalence of Salmonella in flies collected biweekly on an integrated animal and produce operation over two growing seasons. Eleven pooled samples, out of 889, tested positive for Salmonella. Flies from the Calliphoridae, Muscidae, Sarcophagidae, and Tachinidae families were associated with Salmonella carriage, but fly family was not a significant factor for isolation of Salmonella (p=0.303). Fly species were a significant factor (p=0.026), with five Pentacricia aldrichii pools testing positive for Salmonella. With the exception of single specimen isolation, prevalence ranged from 2.2 to 15.2%. With the exception of the Tachinidae family, these results reflect a strong association of flies that are commonly associated with feces or are pests of animals. Trap location was not significantly associated with isolation of Salmonella-positive flies (p=0.236). Overall, the population of flies was not as abundant as studies conducted with produce grown in close proximity to concentrated animal feeding operations, indicating a reduced risk of transmission; however, similar to these studies, fly families that are commonly isolated from fecal and decaying matter were most frequently associated with Salmonella isolation. Further work is warranted to elucidate the foodborne pathogen transmission rates to produce and subsequent survival over time.

Impact of Horizontal Edge–Interior and Vertical Canopy–Understory Gradients on the Abundance and Diversity of Bark and Woodboring Beetles in Survey Traps

Semiochemical-baited intercept traps are important tools used to collect information about the presence/absence and population dynamics of forest insects. The performance of these tools is influenced by trap location along both horizontal edge–interior and vertical understory–canopy gradients. Consequently, the development of survey and detection programs requires both the development of effective traps and semiochemical lures but also deployment protocols to guide their use. We used field trapping experiments to examine the impact of both horizontal edge–interior and vertical understory–canopy gradients and their interactions with the species richness and abundance of Buprestidae, Cerambycidae and Curculionidae. Both gradients had significant effects on the diversity and abundance of all three families collected in traps and the pattern of gradient effects differed between the two experiments. In the first experiment, traps were deployed along transects involving large (>100 m) forest gaps and in the second experiment traps transected small (ca. 15 m) forest gaps. These results were consistent with the idea that gradient effects on the abundance and diversity of these three families of forest Coleoptera are context dependent. The results of this study suggest that monitoring programs for bark and woodboring beetles should deploy traps at multiple locations along both vertical understory–canopy and horizontal edge–interior gradients.

Defining the baseline for river restoration: comparing carabid beetle diversity of natural and human-impacted riparian habitats

Near-natural rivers and riparian ecosystems can represent biodiversity hotspots harbouring many highly specialised, rare and endangered species. During the past centuries, these habitats have been heavily degraded by anthropogenic use, and therefore river restoration is one of the most striking fields of action that is legally defined by the European Union Water Framework Directive. Successful restoration depends on realistic and specified targets that should be defined beforehand and founded on status quo surveys. We present a comparison of carabid beetle communities in riparian habitats of natural and managed river sites of the Mulde River in the Biosphere Reserve Middle Elbe. This endeavour is part of a unique multi-level revitalisation project. Pitfall trapping in 2016 and 2017 yielded 111 carabid species with many species of conservation concern in natural and managed habitats. However, Simpson diversity and functional diversity were lower in the latter. Both habitats harboured specific species assemblages with characteristic indicator species. Additionally, the trap location on slip-off slopes or cut banks was a significant driver of species composition. Our results indicate high ecological development potentials for the Mulde River, but restoration should consider differences between slip-off slopes and cut-off banks. We postulate that future restoration will foster population increases as well as a wider distribution of rare and endangered riparian habitat specialists.

ANALISIS FAKTOR LINGKUNGAN TERHADAP DISTRIBUSI JENIS TIKUS YANG TERKONFIRMASI SEBAGAI RESERVOIR LEPTOSPIROSIS DI TIGA KABUPATEN DI PROVINSI SUMATERA SELATAN

The role of rats as a potential reservoir of the disease remains negative impacts on the routine life, safety, welfare and economic communities. Rattus tanezumi, R. norvegicus, R. exulans, R. hoffmani, R. argentiventer and Maxomys bartelsi have been confirmed as leptospirosis reservoirs in Indonesia. Some rat species suspected as leptospirosis reservoirs were reported in Lahat, Banyuasin and Ogan Komering Ilir (OKI) districts. It puts those three districts on high risk of leptospirosis transmission in the community. The aim of the study was to identify the environmental factors associated with the distribution of the leptospirosis-confirmed rats in the three districts of South Sumatera Province. Data used in this study were obtained from surveillance of the Specific Research on Vector and Reservoir Diseases Special Research" (Rikhus Vektora) conducted in 2015. A total of 308 captured rats was tested for leptospirosis using MAT and PCR methods. The environmental variables were recorded during the surveillance. Data were analysed using chi-square. The laboratory results demonstrated that 44 of 308 rats captured in the three districts were positive for leptospirosis. In addition, there was a significant correlation between the distribution of the positive leptospirosis-confirmed rats with ecosystem variables, trap location, altitudes and temperature. However, they were not correlated to the weather variables and pH. Unfortunately, the topographic variables could not be analysed because all rats were found in the lowlands. The early warning of leptospirosis transmission should be addressed to the three districts due to the presence of the positive leptospirosis-confirmed rats in those areas.

Trap Location and Premises Condition Influences on Aedes aegypti (Diptera: Culicidae) Catches Using Biogents Sentinel Traps During a ‘Rear and Release’ Program: Implications for Designing Surveillance Programs

As the incidence of arboviral diseases such as dengue, Zika, chikungunya, and yellow fever increases globally, controlling their primary vector, Aedes aegypti (L.) (Diptera: Culicidae), is of greater importance than ever before. Mosquito control programs rely heavily on effective adult surveillance to ensure methodological efficacy. The Biogents Sentinel (BGS) trap is the gold standard for surveilling adult Aedes mosquitoes and is commonly deployed worldwide, including during modern ‘rear and release’ programs. Despite its extensive use, few studies have directly assessed environmental characteristics that affect BGS trap catches, let alone how these influences change during ‘rear and release’ programs. We assessed male and female Ae. aegypti spatial stability, as well as premises condition and trap location influences on BGS trap catches, as part of Debug Innisfail ‘rear and release’ program in northern Australia. We found similar trends in spatial stability of male and female mosquitoes at both weekly and monthly resolutions. From surveillance in locations where no males were released, reduced catches were found at premises that contained somewhat damaged houses and unscreened properties. In addition, when traps were located in areas that were unsheltered, more than 10 m from commonly used sitting areas or more visually complex catches were also negatively affected. In locations where males were released, we found that traps in treatment sites, relative to control sites, displayed increased catches in heavily shaded premises and were inconsistently influenced by differences in house sets and building materials. Such findings have valuable implications for a range of Ae. aegypti surveillance programs.