Nanocellulose Production Using Ionic Liquids with Enzymatic Pretreatment

Nanocellulose has gained increasing attention during the past decade, which is related to its unique properties and wide application. In this paper, nanocellulose samples were produced via hydrolysis with ionic liquids (1-ethyl-3-methylimidazole acetate (EmimOAc) and 1-allyl-3-methylimidazolium chloride (AmimCl)) from microcrystalline celluloses (Avicel and Whatman) subjected to enzymatic pretreatment. The obtained material was characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM), and thermogravimetric analysis (TG). The results showed that the nanocellulose had a regular and spherical structure with diameters of 30–40 nm and exhibited lower crystallinity and thermal stability than the material obtained after hydrolysis with Trichoderma reesei enzymes. However, the enzyme-pretreated Avicel had a particle size of about 200 nm and a cellulose II structure. A two-step process involving enzyme pretreatment and hydrolysis with ionic liquids resulted in the production of nanocellulose. Moreover, the particle size of nanocellulose and its structure depend on the ionic liquid used.

Insight into the Evolution of the Cellulose Microstructure Through the Enzyme Pretreatment Method

In this work, the changes of properties and microstructure of cellulose (bleached hardwood kraft pulp (BHKP)) subjected to different enzyme pretreatment times (0–10 h) were explored for further fibrillation. The various properties of the pretreated cellulose gradually decrease with the elapse of time relative to the pristine material, such as yield, water retention value, aspect ratio and degree of polymerization, etc. Enzyme pretreatment can promote the peeling of fibrils and loosen the amorphous areas of cellulose identified by Scanning Electron Microscope (SEM) and X-ray diffraction (XRD). A thorough investigation of the relation between pretreatment and evolution of inter-/intra-molecular H-bonds in cellulose was conducted including content and cleave sequence of H-bonds by Fourier transform infrared spectroscopy (FTIR), second derivative analysis and generalized two-dimensional correlation spectroscopy (2DCOS). The intermolecular H-bonds with the most significant decrease in content was cleaved first relative to the intramolecular H-bonds. These discoveries provide theoretical support to more effective pretreatment method for commercial production of fibrils from cellulosic fibers.

Effect of Pectinolytic Enzyme Pretreatment on the Clarification of Cranberry Juice by Ultrafiltration

Cranberries, mainly processed as juice, have garnered interest over the past decade due to their high content of phytochemical compounds related to promising health benefits. To meet consumer expectations, a juice clarification step is usually incorporated to remove suspended solids. The use of pectinolytic enzyme and membrane processes are commonly applied to the production of clarified juices, but no studies have been done on cranberry juice. In this study, the effects of 60 (D60) and 120 min (D120) of depectinization by pectinolytic enzymes coupled to clarification by ultrafiltration (UF) (membrane molecular weight cut-off (MWCO) of 50, 100 and 500 kDa) was evaluated on the filtration performance, membrane fouling and cranberry juice composition. Compared to fresh juice, depectinization for 60 and 120 min reduced the UF duration by 16.7 and 20 min, respectively. The best filtration performance, in terms of permeate fluxes, was obtained with the 500 kDa MWCO UF membrane despite the highest total flux decline (41.5 to 57.6%). The fouling layer at the membrane surface was composed of polyphenols and anthocyanins. Compared to fresh juice, anthocyanin decreased (44% and 58% for D60 and D120, respectively) in depectinized juices whereas proanthocyanidin (PAC) content increased by 16%. In view of the industrial application, a 60 min depectinization coupled to clarification by a 500 kDa UF membrane could be viewed as a good compromise between the enhancement of filtration performance and the loss of polyphenols and their fouling at the membrane surface.

ENHANCEMENT OF CAFFEINE RECOVERY FROM ROASTED COFFEE POWDER BY ENZYME CELLULASE PRETREATMENT, HOT-WATER EXTRACTION AND CO2-ADDING

The efficiency of caffeine recovery during extraction with highpressure hot-water solvent was investigated to find out the conditions for maximum caffeine recovery in roasted coffee powder. In addition, to support the extraction process with high-pressure hot-water solvent, cellulase enzyme pretreatment of roasted coffee powder is also applied and has demonstrated the supporting role of the enzyme in improving caffeine extraction efficiency. CO2 can participate in the extraction process with the role of changing the pH of the solvent at the high temperature and obtained positive results. In the presence of the enzyme cellulase at avalue of 8 UI/g, the pretreatment time was 90 minutes; the extraction process performed at 110oC for temperature and 10 min for the extraction time, the efficiency of caffeine recovery can reach 96,36 ±2,56%.

Effect of nanofibrillated cellulose made from enzyme-pretreated bamboo pulp on paper strength

The applicability of bleached bamboo kraft pulp (Ba-BKP) was explored as a raw material for the manufacture of nanofibrillated cellulose (EN-NFC) made of enzyme-pretreated pulps and the effects of the EN-NFC on enhancing paper strength. The Ba-BKP was pretreated using an endo-glucanase enzyme at 50 °C and pH 6, after which the EN-NFC was made by micro-grinding. Bleached hardwood kraft pulp (Hw-BKP) was used as a control, and the non-enzymatic refining pretreatment of BKPs was compared with the enzyme pretreatment. The EN-NFC was incorporated into handsheets, and the sheet strengths were measured. The physical properties of the NFC made from the Ba-BKP were similar to those made from the Hw-BKP. The NFC prepared following enzyme pretreatment were smaller and more uniform than those pretreated with refining. The EN-NFC made from the Ba-BKP was effective at enhancing tensile index by 52.7%, and burst index by 210.2% when 2% of EN-NFC was added in the furnish, and those of handsheets containing the EN-NFC made from Hw-BKP showed the similar improvement. Therefore, Ba-BKP can be used as a raw material for the manufacture of EN-NFC that confers similar physical properties and strength enhancement to paper as those made from Hw-BKP.

Biobleaching: An eco-friendly approach to reduce chemical consumption and pollutants generation

The pulp and paper industry is known to be a large contributor to environmental pollution due to the huge consumption of chemicals and energy. Several chemicals including H2SO4, Cl2, ClO2, NaOH, and H2O2 are used during the bleaching process. These chemicals react with lignin and carbohydrates to generate a substantial amount of pollutants in bleach effluents. Environmental pressure has compelled the pulp and paper industry to reduce pollutant generation from the bleaching section. Enzymes have emerged as simple, economical, and eco-friendly alternatives for bleaching of pulp. The pretreatment of pulp with enzymes is termed as biobleaching or pre-bleaching. Different microbial enzymes such as xylanases, pectinases, laccases, manganese peroxidases (MnP), and lignin peroxidases are used for biobleaching. Xylanases depolymerize the hemicelluloses precipitated on pulp fiber surfaces and improves the efficiency of bleaching chemicals. Xylanase treatment also increases the pulp fibrillation and reduces the beating time of the pulp. Pectinases hydrolyze pectin available in the pulp fibers and improve the papermaking process. Laccase treatment is found more effective along with mediator molecules (as a laccase-mediator system). Biobleaching of pulp results in the superior quality of pulp along with lower consumption of chlorine-based chemicals and lower generation of adsorbable organic halidesadsorbable organic halides (AOX. An enzyme pretreatment reduces the kappa number of pulp and improves ISO brightness significantly. Better physical strength properties and pulp viscosity have also been observed during biobleaching of pulp.