A Major Challenges Advanced Underground Drainage Monitoring and Automatic Rescue System using Internet of Things

In this project, An Advanced Underground Drainage Monitoring And Automatic Rescue System using Internet of Things is proposed to protect the sanitation workers form health issues. In India sewage can be cleaned from manholes and drains are a difficult and risky task for anyone, but these people/workers are forced to do these jobs just to earn for their family economy. In order to overcome this issues, a new device is proposed to monitor the human health while entering into the sewage and provide the health parameters in the real time to the officials outside or the control room. Particularly, blood pressure of sanitation workers and toxic gas level of drainage is monitored using this new proposed rescue system. The main component of this system is Arduino controller. There are three types of sensors such as Toxic gas sensor, Methane gas sensor and Ultrasonic sensor used for the proposed system. Water level indicator is used when drainage is full to find from ultrasonic sensor, SMS will be send through GSM technology. Methane Gas and Toxic gas sensor level is high, Buzzer will be ON at the same time location will be share automatically with the help of GPS. The received sensor details display on LCD at receiver with help of GSM. The performance of proposed system is compared with other existing system and shown to be more effective in terms of protect the sanitation workers from health issues.

Tornádo 2021 v České republice – psychosociální pomoc IZS v zasažených oblastech

The tornado hit the territory of several municipalities in South Moravia in the evening on Thursday, June 24, 2021. Although it was a relatively small area, the extent of the damage was extraordinary and of unprecedented proportions. Many people found themselves in direct danger of themselves and their loved ones. In psychology, we call such an event potentially traumatic, and we know from experience that it can have serious effects on the human psyche. The article aims to present how the system of psychosocial crisis intervention and psychological first aid provided by the Integrated Rescue System of the Czech Republic worked. This psychosocial assistance in the acute phase of a disaster builds the core of psychosocial help. There is a link to other organizations, which have their function mainly in the stabilization phase, in the medium and long-term care of the affected persons. We also consider it necessary to repeat the principles of psychosocial assistance in emergencies.

Safety Approaches of Population Protection During Air Shows in the Czech Republic

In recent years, organizing air shows is a very popular attraction. The proof is the high number of visitors. Examples are the NATO Days in Ostrava, where the number of visitors is around 200,000, or the CIAF in Hradec Králové, which will be attended by 40,000 visitors. The aim of the articles is to approach and analyse the approaches that ensure the safety of people during these events in the Czech Republic. Several legal regulations dealing with this issue were analysed and their content is focused on the obligations of the rescue system during air events. The contribution of the article can be information that can help ensure the safety of air shows abroad.

Population Variability Generated during Rescue Process and Passaging of Recombinant Mumps Viruses

Recombinant mumps viruses (MuVs) based on established vaccine strains represent attractive vector candidates as they have known track records for high efficacy and the viral genome does not integrate in the host cells. We developed a rescue system based on the consensus sequence of the L-Zagreb vaccine and generated seven different recombinant MuVs by (a) insertion of one or two additional transcription units (ATUs), (b) lengthening of a noncoding region to the extent that the longest noncoding region in MuV genome is created, or (c) replacement of original L-Zagreb sequences with sequences rich in CG and AT dinucleotides. All viruses were successfully rescued and faithfully matched sequences of input plasmids. In primary rescued stocks, low percentages of heterogeneous positions were found (maximum 0.12%) and substitutions were predominantly obtained in minor variants, with maximally four substitutions seen in consensus. ATUs did not accumulate more mutations than the natural MuV genes. Six substitutions characteristic for recombinant viruses generated in our system were defined, as they repetitively occurred during rescue processes. In subsequent passaging of primary rescue stocks in Vero cells, different inconsistencies within quasispecies structures were observed. In order to assure that unwanted mutations did not emerge and accumulate, sub-consensus variability should be closely monitored. As we show for Pro408Leu mutation in L gene and a stop codon in one of ATUs, positively selected variants can rise to frequencies over 85% in only few passages.

Evaluation of Emergency Rescue Efficiency in Coal Mines Based on Co-entropy

By decomposing the coal mine emergency rescue organization and coal mine emergency rescue work, the synergy entropy function is constructed by combining the synergy class with the entropy theory, and the synergy efficiency assessment model of coal mine emergency rescue is established. Analyze the synergistic relationship between departments in the rescue organization and between departments and functional units, construct the synergistic influence matrix, and obtain the synergistic status of the coal mine emergency rescue system by calculating the synergistic entropy, synergistic efficiency and synergistic degree. The quantitative analysis of the emergency rescue system in coal mines is achieved by analyzing the situation of the rescue work in coal mines and evaluating the rescue organizations at each tier based on the established evaluation model.

Construction of an Influenza D Virus with an Eight-Segmented Genome

Influenza D virus (IDV) may cause the bovine respiratory disease complex, which is the most common and costly disease affecting the cattle industry. Previously, we revealed that eight segments could be actively packaged in its single virion, suggesting that IDV with the seven-segmented genome shows an agnostic genome packaging mechanism. Herein, we engineered an eight-segmented recombinant IDV in which the NS1 or NS2 genes were separated from NS segment into independent segments (NS1 or NS2 segments, respectively), leading to monocistronic translation of each NS protein. We constructed two plasmids: one for the viral RNA (vRNA)-synthesis of the NS1 segment with a silent mutation at the splicing acceptor site, which controls NS2 transcription in the NS segment; and another for the RNA synthesis of the NS2 segment, with deletion of the intron in the NS segment. These plasmids and six other vRNA-synthesis plasmids were used to fabricate an infectious eight-segmented IDV via reverse genetics. This system enables analysis of the functions of NS1 or NS2. We tested the requirement of the N-terminal overlapping region (NOR) in these proteins for viral infectivity. We rescued a virus with NOR-deleted NS2 protein, which displayed a growth rate equivalent to that of the eight-segmented virus with intact NS2. Thus, the NOR may not influence viral growth. In contrast, a virus with NOR-deleted NS1 protein could not be rescued. These results indicate that the eight-segmented rescue system of IDV may provide an alternative method to analyze viral proteins at the molecular level.

Comprehensive Analysis of Housing Estate Infrastructure in Relation to the Passability of Firefighting Equipment

The article focuses on the assessment and evaluation of the passability in densely populated parts of cities with multi-storey housing estates, in terms of the operation of the integrated rescue system (IRS) in the Czech Republic. The aim of the research is to minimize the arrival times to conduct the intervention as efficiently as possible. The presented problem is caused by unsystematic development of housing estates and the emergence of secondary problems in the form of inability to reach the place of intervention by the larger IRS vehicles. The vision presented in this document presents a systematic approach to improve the serviceability of individual blocks of flats. The main aim is to ensure passability, even for the largest equipment, such as fire engine ladders. Detailed mapping of the selected sites by drones, construction of their digital model, and subsequent virtual verification of the passability by specific vehicle models on identified access roads was performed. The results obtained by this procedure can then be implemented in the navigation of the fire safety forces and facilitate their arrival at the site of intervention. At the end, specific ways are presented in which the whole system can be modified to be able to intuitively change and choose individual access routes in real time, based on the current situation in the area.

A DREaMR system to simplify combining mutations with rescue transgenes in Aedes aegypti

In most experimental animals, it is challenging to combine mutations and rescue transgenes and to use bipartite systems to assess gene expression. To circumvent the difficulties in combining multiple genetic elements, we developed the DREaMR ( Drug-on, REporter, Mutant, Rescue) system. Using Drosophila white as the initial model, we demonstrated that introduction of a single insertion by CRISPR/Cas9 created a null mutation, a tagged rescue construct, which could be induced with doxycycline, and which allowed assessment of protein expression. To create a DREaMR in an organism in which combining multiple genetic elements is more problematic than in Drosophila, we tested the mosquito, Aedes aegypti—the insect vector for dengue, yellow fever, Zika and other viral diseases. We generated a DREaMR allele in the kh gene, which permitted us to induce expression of the rescue construct, and detect expression of Kh. Thus, this system avoids the need to perform genetic crosses to introduce an inducible rescue transgene in a mutant background, or to combine driver and reporter lines to examine expression of the targeted protein. We propose that DREaMR provides a system that can be applied to additional mosquito vectors as well as other organisms in which CRISPR/Cas9 is effective.