The Succession of the Gut Microbiota in Insects: A Dynamic Alteration of the Gut Microbiota During the Whole Life Cycle of Honey Bees (Apis cerana)

The Asian honey bee Apis cerana is a valuable biological resource insect that plays an important role in the ecological environment and agricultural economy. The composition of the gut microbiota has a great influence on the health and development of the host. However, studies on the insect gut microbiota are rarely reported, especially studies on the dynamic succession of the insect gut microbiota. Therefore, this study used high-throughput sequencing technology to sequence the gut microbiota of A. cerana at different developmental stages (0 days post emergence (0 dpe), 1 dpe, 3 dpe, 7 dpe, 12 dpe, 19 dpe, 25 dpe, 30 dpe, and 35 dpe). The results of this study indicated that the diversity of the gut microbiota varied significantly at different developmental stages (ACE, P = 0.045; Chao1, P = 0.031; Shannon, P = 0.0019; Simpson, P = 0.041). In addition, at the phylum and genus taxonomic levels, the dominant constituents in the gut microbiota changed significantly at different developmental stages. Our results also suggest that environmental exposure in the early stages of development has the greatest impact on the gut microbiota. The results of this study reveal the general rule of gut microbiota succession in the A. cerana life cycle. This study not only deepens our understanding of the colonization pattern of the gut microbiota in workers but also provides more comprehensive information for exploring the colonization of the gut microbiota in insects and other animals.

Nosema ceranae - a new threat to honey bees (Apis mellifera)?

Back in 1900 already, the Microsporidium Nosema apis was described inApis mellifera. Thereby the Nosemosis remains without symptoms in the beehive to a certain degree. Studies indicate that infected bees have a shortened lifespan, due to a series of changes in physiological parameters. The consequence of these changes are diarrheal symptoms and the spread of infectious spores in thehive. There is also a seasonal infection course observed, which has its peak in spring time (April, May). Colloquially, the Nosemosis is therefore also known as spring shrinking craze. More recently, a new Nosema species in the European honey bee has been described, where a host-switch from the Asian honey bee A. cerana to A. mellifera has occurred. N. ceranae is blamed for colony losses in the south of Spain, many general colony losses during wintertime in Europe and has also a contribution to the Colony Collapse Disorder (CCD) in the U.S. It seems likely that the original Nosema species (N. apis) is displaced more and more by N. ceranae for unknown reasons. Within the EU project „BEE DOC“, monitoring studies on colonies in southern Germany, Switzerland, southern France, Sweden and Finland were performed. Although the high prevalence of N. ceranae could be confirmed, no increased colony mortality due to Nosemosis was recorded. This was also observed by other colleagues and thus the „new threat“ is open to debate.

Physiological Analysis and Transcriptome Analysis of Asian Honey Bee (Apis cerana cerana) in Response to Sublethal Neonicotinoid Imidacloprid

Asian honey bee (Apis cerana) is the most important Chinese indigenous species, while its toxicological characteristic against neonicotinoids is poorly known. Here, we combined physiological experiments with a genome-wide transcriptome analysis to understand the molecular basis of genetic variation that responds to sublethal imidacloprid at different exposure durations in A. cerana. We found that LC5 dose of imidacloprid had a negative impact on climbing ability and sucrose responsiveness in A. cerana. When bees were fed with LC5 dose of imidacloprid, the enzyme activities of P450 and CarE were decreased, while the GSTs activity was not influenced by the pesticide exposure. The dynamic transcriptomic profiles of A. cerana workers exposed to LC5 dose of imidacloprid for 1 h, 8 h, and 16 h were obtained by high-throughput RNA-sequencing. We performed the expression patterns of differentially expressed genes (DEGs) through trend analysis, and conducted the gene ontology analysis and KEGG pathway enrichment analysis with DEGs in up- and down-regulated pattern profiles. We observed that more genes involved in metabolism, catalytic activity, and structural molecule activity are down-regulated; while more up-regulated genes were enriched in terms associated with response to stimulus, transporter activity, and signal transducer activity. Additionally, genes related to the phenylalanine metabolism pathway, FoxO signaling pathway, and mTOR signaling pathway as indicated in the KEGG analysis were significantly up-related in the exposed bees. Our findings provide a comprehensive understanding of Asian honey bee in response to neonicotinoids sublethal toxicity, and could be used to further investigate the complex molecular mechanisms in Asian honey bee under pesticide stress.

Varroa destructor: how does it harm Apis mellifera honey bees and what can be done about it?

Since its migration from the Asian honey bee (Apis cerana) to the European honey bee (Apis mellifera), the ectoparasitic mite Varroa destructor has emerged as a major issue for beekeeping worldwide. Due to a short history of coevolution, the host–parasite relationship between A. mellifera and V. destructor is unbalanced, with honey bees suffering infestation effects at the individual, colony and population levels. Several control solutions have been developed to tackle the colony and production losses due to Varroa, but the burden caused by the mite in combination with other biotic and abiotic factors continues to increase, weakening the beekeeping industry. In this synthetic review, we highlight the main advances made between 2015 and 2020 on V. destructor biology and its impact on the health of the honey bee, A. mellifera. We also describe the main control solutions that are currently available to fight the mite and place a special focus on new methodological developments, which point to integrated pest management strategies for the control of Varroa in honey bee colonies.

Varroa destructor: A Complex Parasite, Crippling Honey bees Worldwide

The parasitic mite, Varroa destructor, has shaken the beekeeping and pollination industries since its spread from its native host, the Asian honey bee (Apis cerana), to the naïve European honey bee (A. mellifera) used commercially for pollination and honey production around the globe. Varroa is the greatest threat to honey bee health. Worrying observations include increasing acaricide resistance in the varroa population and sinking economic treatment thresholds, suggesting that the mites or their vectored viruses are becoming more virulent. Highly infested weak colonies facilitate mite dispersal and disease transmission to stronger and healthier colonies. Here, we review recent developments in the biology, pathology and management of varroa, and integrate older knowledge that is less well known.

Similarities in the behaviour of dance followers among honey bee species suggest a conserved mechanism of dance communication

Group living organisms rely on intra-group communication to adjust individual and collective behavioural decisions. Complex communication systems are predominantly multimodal and combine modulatory and information bearing signals. The honey bee waggle dance, one of the most elaborate forms of communication in invertebrates, stimulates nestmates to search for food and communicates symbolic information about the location of the food source. Previous studies on the dance behaviour in diverse honey bee species demonstrated distinct differences in the combination of visual, auditory, olfactory, and tactile signals produced by the dancer. We now studied the behaviour of the receivers of the dance signals, the dance followers, to explore the significance of the different signals in the communication process. In particular, we ask whether there are differences in the behaviour of dance followers between the 3 major Asian honey bee species, A. florea, A. dorsata and A. cerana, and whether these might correlate with the differences in the signals produced by the dancing foragers. Our comparison demonstrates that the behaviour of the dance followers is highly conserved across all 3 species despite the differences in the dance signals. The highest number of followers was present lateral to the dancer throughout the waggle run, and the mean body orientation of the dance followers with respect to the waggle dancer was close to 90° throughout the run for all 3 species. These findings suggest that dance communication might be more conserved than implied by the differences in the signals produced by the dancer. Along with studies in A. mellifera, our results indicate that all honey bee species rely on tactile contacts between the dancer and follower to communicate spatial information. The cues and signals that differ between the species may be involved in attracting the followers towards the dancer in the different nest environments.

Biology and Management of Varroa destructor (Mesostigmata: Varroidae) in Apis mellifera (Hymenoptera: Apidae) Colonies

Varroa mite (Varroa destructor Anderson and Trueman) infestation of European honey bee (Apis mellifera L.) colonies has been a growing cause of international concern among beekeepers throughout the last 50 yr. Varroa destructor spread from the Asian honey bee (Apis cerana Fabricius [Hymenoptera: Apidae]) to A. mellifera populations in Europe in the 1970s, and subsequently traveled to the Americas. In addition to causing damage through feeding upon lipids of larval and adult bees, V. destructor also facilitates the spread of several viruses, with deformed wing virus being most prevalent. Several sampling methods have been developed for estimating infestation levels of A. mellifera colonies, and acaricide treatments have been implemented. However, overuse of synthetic acaricides in the past has led to widespread acaricide resistant V. destructor populations. The application of Integrated Pest Management (IPM) techniques is a more recent development in V. destructor control and is suggested to be more effective than only using pesticides, thereby posing fewer threats to A. mellifera colonies. When using IPM methods, informed management decisions are made based upon sampling, and cultural and mechanical controls are implemented prior to use of acaricide treatments. If acaricides are deemed necessary, they are rotated based on their mode of action, thus avoiding V. destructor resistance development.