PSXV-13 The activity of thiol cathepsins in seminal plasma of stallions with different percentages of viable sperm after freezing-thawing

The aim of this work was to study the activity of cathepsins B, L and H in the seminal plasma of stallions with normal (>25%, n = 11) and low (< 25%, n = 13) percentage of viable sperm after freezing-thawing. Sperm of 24 Arabian stallions aged from 5 to 20 years (12.1 ± 4.8 years on average) was collected during the breeding season (February-May). The activity of cathepsins in spermoplasm was studied by the spectrofluorometric method (System 3 Scanning Spectrofluorometry, Optical technology devices, inc. Elmstord, New York, 10523) by Barrett&Kirschke. The viability of the sperm was determined after its freezing-thawing. Sperm smears were eosin stained and live:dead-ratio was examined using an Olympus BX41 phase contrast microscope (Olympus Corporation, Japan). The significance of differences in the studied groups was determined using the Mann-Whitney U-test. There were no significant differences in the activity of catepsins B and L in the spermoplasm of stallions with normal and low percentages of viable spermatozoa after freezing-thawing. It was found that in the group of stallions with a normal percentage of viable spermatozoa after freezing-thawing, the activity of cathepsin H in the spermoplasm was significantly higher than in the group with a low percentage of viable spermatozoa (P = 0.0219). Free radicals formed during freezing-thawing of sperm can damage cell membranes, leading to loss of sperm viability. Thiol cathepsins are involved in the degradation of oxidatively modified proteins and, apparently, it is cathepsin H that is most actively involved in this process in the sperm of stallions. We assume that the low activity of cathepsin H in the seminal plasma of stallions with a low percentage of viable spermatozoa indicates a high involvement of this enzyme in the protection of sperm membranes from oxidative damage. The research was supported by the Russian Science Foundation grant No. 20-16-00101.

Cytologgy screening of Nigerian ecotype goats for reproductive potentialities

X-chromatin (drumstick) screening evaluated through the identification of the drumstick appendages of neutrophilis was carried on Nigerian goat breeds. 150 weaner bucks and 150 weaner does were used for the study. Some of the animals exhibited aberrant x-chromatin while some showed normal percentage of incidence of the drumstick. Both genotype and sex differences had significant effect (P>0.05) on the x-chromatin in genotype III (RSG * WAD), Genotype III design should not be used for reproductive purpose except on the ground of fattening for slaughter

IPEX Syndrome with Normal FOXP3 Protein Expression in Treg Cells in an Infant Presenting with Intractable Diarrhea as a Single Symptom

IPEX (immune dysregulation-polyendocrinopathy-enteropathy-X-linked) syndrome is a rare, potentially fatal multisystem disorder caused by mutations in the FOXP3 gene. This can lead to quantitative or functional deficiency of regulatory T cells (Treg), thereby affecting their immune-suppressive actions which can in turn cause autoimmune and inflammatory disorders. We describe an infant with IPEX syndrome with unremarkable maternal family history whose only presentations were severe diarrhea and malnutrition. The patient had a normal percentage of Treg cells and FOXP3 protein expression, but further testing revealed a hemizygous missense mutation in the FOXP3 gene. IPEX syndrome should be considered in young children even if severe intractable diarrhea is the only symptom with no other autoimmune manifestations. Sequencing of the FOXP3 gene should always be considered for accurate diagnosis to look for mutations even in the face of normal FOXP3 protein expression in the Treg cell.

The Influence of Nutrition on the Percentage of Fat Tissue among Students of University of Physical Education in Krakow

Introduction: Adequate nutrition is one of the most important environmental factors that have an influence on human development and maintaining good health condition. It consists in meeting the demand of an organism for energy and all the necessary nutrients completely. Material and methods: The survey was conducted in 2014. It covered 93 second year Physiotherapy and Physical Education students (48 females and 45 males) in Bronisław Czech University of Physical Education in Kraków. The average age of the surveyed students was 21. The Tanita scales were applied to define the value of BMI and to calculate the percentage of fat tissue in the organism. Results: The research results have revealed that the respondents represent an average and good manner of nutrition. The majority of the subjects (73.1%) had a normal percentage of fat tissue. While examining the relations between the way of nutrition and fat percentage, it was found that 50% of females who fed themselves well had a low FAT%. Only 13.7% of males representing an average interval of nutrition manner were found to have a high percentage of fat tissue. Among the majority of the respondents (79.6%) the BMI level was within the norm. Overweight was more frequent among males than females, whereas underweight vice versa. Conclusions: The majority of the surveyed students feed themselves in an average manner. The manner of nutrition had a significant influence on body fat only among females. The majority of respondents’ BMI level was within the norm.

Histological study of the influence of antidiabetic plant collection on pancreatic tissue in rats with diabetes mellitus, which is induced by dexamethasone

Diabetes mellitus is a global problem today, because there is a sharp increase the number of patients in the world each year. Severe complications of diabetes are very dangerous because it can lead to disability of patients and high mortality. The aim of our work was to study the protective effect of antidiabetic plant collection on the pancreas in rats with diabetes type 2, caused by the introduction of dexamethasone. The research materials were pancreatic tissue of rats with experimental diabetes that received the decoction (1:10) of antidiabetic plant collection (witch includes Equiseti arvensis herba, Sambuci flores, Inulae rhizomata et radices, Hyperici herba, Tiliae flores, Polygoni avicularis herba, Myrtilli folium, Urticae folia) in treatment and preventive mode. The model of diabetes mellitus was induced by administration of dexamethasone 0.125 ml/kg for 15 days. Decoction of antidiabetic plant collection in doses 6 ml/kg, 9 ml/kg and 12 ml/kg reduces diabetogenic properties of dexamethasone when administered simultaneously. The insular tissue increases absorbance pancreatic islets, to some extent restored the normal percentage distribution of the islets in size, histologically visualized the increase more high-grade cells. The study found that antidiabetic plant collection reduces destructive-dystrophic changes of insular apparatus in rats with diabetes type 2. Antidiabetic effect of the studied plant collection has dose-dependent nature. The most effective conditional-effective doses are 9 and 12 ml/kg.

Aberrant Peripheral Immune Function in a Good Syndrome Patient

Good’s syndrome (GS) is often accompanied by recurrent respiratory infections and chronic diarrhea. The main purpose was to evaluate the peripheral immune status of a GS patient after thymoma resection. Twenty healthy volunteers were recruited as healthy controls (HCs). Flow cytometry was applied to determine the proportions of circuiting CD4+ T cells, CD8+ T cells, γδT cells, and regulatory T (Treg) cells in our GS patient. We also examined the proliferation capability of ex vivo CD4+ T cells and detected the levels of cytokines interferon- (IFN-) γ and interleukin-17A secreted by ex vivo immune cells from this GS patient. Compared with healthy control subjects, this GS patient had fewer B cells, an inverted ratio of CD4+/CD8+ cells, and more Treg cells in his peripheral blood. Additionally, the patient’s Vδ2 T cell levels were significantly decreased despite having a normal percentage of γδT cells. Ex vivo peripheral CD4+ T cells from the patient showed insufficient proliferation and division potential as well as excessive expression of PD-1. Moreover, IFN-γ was predominantly derived from CD8+ T cells in this GS patient, rather than from CD4+ T cells and γδT cells. This GS patient had impaired T and B cell immunological alternations and cytokine disruptions after thymectomy. Detailed research should focus on therapies that can adjust the immune status in such patients for a better outcome.

The Effect of Consumption of Dried Spinach (Spinacia oleracea) on Level of Hemoglobin and Uric Acid for Sample of Women.

This study been conducted and applied in Alrashed Health Center/Mahmodia Sector/ kerigh Sector/Ministry of Health/Baghdad City, and conducted on samples of women who are suffering from iron deficiency (Hemoglobin%) in blood for the year 2013. Fifty women been selected (married, unmarried), their ages ranging between 19-40 years old, they been given dried grinding spinach tablet. The dose been given was 3 tablet/day/4-6 weeks, after taken the percentage of Hemoglobin and Uric acid for all studied samples before and after dried spinach tablet given. It was appeared from samples analysis primarily and statically for 50 women, that 4% upon them only suffering high Iron deficiency (8.0-9.9 mg/100ml), 38% upon them suffering from middle iron deficiency (9.0-9.9 mgl/100ml), 46% upon them who suffering from little blood Iron deficiency (10.0-10.9 mgl100ml), and only 12% % upon them who suffering from slight blood Iron deficiency (11.0-11.9 mg/100ml). Also it was appeared that none of the women suffering from high level of Uric acid in their blood.The results of the experiment declare a significant difference due to the dose of tablets dried Spinach (3 tablet/day/ 4-6 weeks), which increase the percentage of Hemoglobin in the blood by 1-1.5 degree/ week and reached the optimum percentage 12 degree and up in 4-6 weeks, with no acidity, and the undesirable test which using the medicine (Ferro-Folic tablets). Although of the above, taking of Spanish tablets doesn't effect on much increasing the percentage of Uric acid, but in small percentage and all of it lower than the normal percentage which is (7.3 mg/100ml).From all of the above we can conclude, that we are able to manufacture the grinded dried Spinach tablet and to be used as medicine to treat Iron deficiency cases and to be given to pregnant women especially in the first three months of pregnancy, due to the disability of taken the chemical medicine, due to the probable distortion happen for the fetus, and also to the individuals who are suffering from Hemolytic anemia. So, for all the above facts, this type of studies and researches should be supported and encouraged the National medicine products against the imported chemical medicine.

Patterns of Ministry of Clergy Married to Clergy in the Church of England

This article argues that for good practice, well-being and fruitful ministry, decisions by and about clergy married to clergy (CMC) in the Church of England require a clear quantitative picture of their ministry, and offers such a picture in early 2013 drawn primarily from published data, compared with national Church of England statistics. Over 26 per cent more clergy dyads were found than previously thought, with many active in ministry. A wide variety of ministry patterns were identified, including a higher than normal percentage in non-parochial roles, supporting previous research noting high levels of boundary enmeshment and absorptiveness. Considerable gender inequality prevailed in shared parochial settings in spite of women having been ordained priest for nearly 20 years, with very few wives holding more senior positions than their husbands, while female CMC are more likely to be dignitaries than other ordained women.

Ameliorating effects and mechanisms of electroacupuncture on gastric dysrhythmia, delayed emptying, and impaired accommodation in diabetic rats

The aim of this study was to investigate the effects and mechanisms of electroacupuncture (EA) on gastric accommodation, gastric dysrhythmia, and gastric emptying (GE) in streptozotocin (STZ)-induced diabetic rats. Five experiments were performed in five groups of STZ-induced diabetic rats to study the effects of EA at ST-36 (Zusanli) on gastric slow-wave dysrhythmia, delayed GE and intestinal transit, impaired gastric accommodation, and the mechanisms of EA involving the autonomic and opioidergic pathways. We found the following: 1) EA improved gastric dysrhythmia in the diabetic rats. The normal percentage of slow waves was 55.4 ± 2.9% at baseline and significantly increased to 69.2 ± 2.2% with EA ( P = 0.01); this effect was blocked by naloxone. 2) EA resulted in a 21.4% increase in GE and 18.2% increase in small intestinal transit in the diabetic rats. 3) EA restored diabetes-induced impairment in gastric accommodation. Gastric accommodation was 0.98 ± 0.13 ml with sham EA and significantly increased to 1.21 ± 0.15 ml with EA ( P = 0.01), and this effect was blocked by naloxone. 4) EA increased vagal activity assessed by the spectral analysis of the heart rate variability. We concluded that EA at ST-36 improves gastric dysrhythmia, delayed GE and intestinal transit, and impaired accommodation in STZ-induced diabetic rats, and the improvement seems to be mainly mediated via the vagal pathway. EA may have a promising therapeutic potential for diabetic gastroparesis.