The EMT activator ZEB1 accelerates endosomal trafficking to establish a polarity axis in lung adenocarcinoma cells

Epithelial-to-mesenchymal transition (EMT) is a transcriptionally governed process by which cancer cells establish a front-rear polarity axis that facilitates motility and invasion. Dynamic assembly of focal adhesions and other actin-based cytoskeletal structures on the leading edge of motile cells requires precise spatial and temporal control of protein trafficking. Yet, the way in which EMT-activating transcriptional programs interface with vesicular trafficking networks that effect cell polarity change remains unclear. Here, by utilizing multiple approaches to assess vesicular transport dynamics through endocytic recycling and retrograde trafficking pathways in lung adenocarcinoma cells at distinct positions on the EMT spectrum, we find that the EMT-activating transcription factor ZEB1 accelerates endocytosis and intracellular trafficking of plasma membrane-bound proteins. ZEB1 drives turnover of the MET receptor tyrosine kinase by hastening receptor endocytosis and transport to the lysosomal compartment for degradation. ZEB1 relieves a plus-end-directed microtubule-dependent kinesin motor protein (KIF13A) and a clathrin-associated adaptor protein complex subunit (AP1S2) from microRNA-dependent silencing, thereby accelerating cargo transport through the endocytic recycling and retrograde vesicular pathways, respectively. Depletion of KIF13A or AP1S2 mitigates ZEB1-dependent focal adhesion dynamics, front-rear axis polarization, and cancer cell motility. Thus, ZEB1-dependent transcriptional networks govern vesicular trafficking dynamics to effect cell polarity change.

Transient Response of the Acid-base Diode for Polarity Change

An application of the so-called acid-base diode would be the sensitive detection of nonhydrogen cations in an acidic medium based on salt-effects. For diode purposes different connecting elements between the acidic and aqueous reservoirs of the diode were developed, namely a polyvinyl alcohol (PVA) hydrogel cylinder, and a polyvinyl butyral (PVB) membrane. During the measurement of the voltage – current characteristic (VCC) of the diode, it was found, that in the case of PVA gel cylinder an overshoot (a local maximum followed by a local minimum) appeared in the time vs. current curve, while the diode was switched between modes (open or closed), that is the direction of the applied voltage was reversed. The overshoot did not appear in PVB membrane.The existence of overshoots was studied by numerical simulations. The time response of the diode with different hypothetic connecting elements was investigated, when the diode was switched between modes via changing the polarity of applied voltage. We found that larger diffusion coefficients of hydrogen and hydroxide ions explain the appearance of overshoots. By examining the concentration and potential profiles a qualitative explanation of this phenomenon was given.

Employee Performance Evaluation Using Sentiment Analysis

Emotional analysis and data mining has become a hot topic in the field of data mining and natural language analysis as a solidly typed mining activity to analyze the concept of objects (i.e., emotion) expressed in the text. Emotional analysis is an important step in the recommendation process, because it allows you to separate the sense of the root context (e.g., positive or negative). In emotional analysis, the word-of-word (BOW) model is widely used in text classification, similar to how it is used in the modeling of a traditional theme. These two anti-emotional texts are considered very similar to the BOW representation. That is why, as a result of polarity change, machine learning methods often fail. We recommend combining a semantic analysis program with a separator to evaluate work results.

REARRANGEMENT OF THE CONFORMATIONAL STRUCTURE OF POLYAMPHOLYTES ON THE SURFACE OF A METAL NANOWIRE IN A TRANSVERSE MICROWAVE ELECTRIC FIELD

Molecular dynamics has been employed to study the rearrangement of the conformational structure of polyampholytes adsorbed on the surface of a gold nanowire with a periodic change in time of its polarity in the transverse direction at an ultrahigh frequency. The radial distributions of the atomic density of the polypeptide and its angular distributions on the nanowire surface have been calculated. At high temperatures, temporary fluctuations in the conformational structure of the adsorbed polyampholyte polypeptide were observed. In this case, for half the period of the nanowire polarity change, the macrochain conformation changed from dense enveloping of the nanowire to an elongated conformational structure along the dipole moment of the nanowire. At low temperature and the nanowire dipole moment, the swelling of the fringe of the adsorbed polyampholyte was observed with a displacement of most of its links to one side with respect to the plane perpendicular to the direction of the nanowire dipole moment and passing through its axis. At low temperature and high values of the nanowire dipole moment, the polyampholyte polypeptide was desorbed from the nanowire surface. An analytical model of conformational rearrangements of a polyampholyte Gaussian chain in the form of an external field perturbation theory is presented.

Critical role of the structure of the mineral-water interface in the zeta potential measured by streaming potential method

<p><span>In this study, zeta potential has been measured by using the streaming potential method for the intact sandstone in contact with CaCl<sub>2</sub> electrolytes. The experimental results show that a positive zeta potential has been observed for the first time for the intact Fontainebleau sandstone under high salinity of CaCl<sub>2</sub>, and its magnitude increases with increasing ionic strength. It cannot be explained by the Gouy-Chapman theory anticipating a constant potential for high salinities due to the collapse of the electrical double layer. Meanwhile, the brine effluents after the completion of the streaming potential measurements were collected and then pH and brine composition were analysed suggesting that those variations of pH and chemical composition are negligible and cannot explain the polarity change at high salinity. The anomalous positive potential of the intact Fontainebleau sandstone is due to that overcharge of calcium ions sorbed into the mineral surface, which is consistence with previous literature data.</span></p>

Resonant coupling of mode-1 and mode-2 internal waves by topography

<p>We consider the resonant coupling of mode-1 and mode-2 internal waves by topography. The mode-2 wave is generated by a mode-1 internal solitary wave encountering variable topography in the framework of a pair of coupled Korteweg-de Vries (KdV) equations.  Three cases (A) weak resonant coupling, (B) moderate resonant coupling, (C) strong resonant coupling, are examined using a three-layer fluid system with fixed total depth but different  layer thicknesses, and each case has two  different topographic slopes, gentle and steep, respectively.  The criterion for the strength of the resonant coupling is  the ratio of the  linear phase speeds c<sub>2 </sub>for mode-2 and c<sub>1</sub> for mode-1 waves.  This ratio c<sub>2</sub>/c<sub>1</sub> varies  from 0.42-0.48 (A), 0.58-0.72 (B), to 0.44-0.92 (C). The simulations using the coupled KdV model are compared with a KdV model for the evolution of a mode-1 wave alone. In case (A) a convex mode-2 wave of small amplitude is generated by a depression incident mode-1 wave and the feedback on mode-1 wave is negligible. In case (B) a concave mode-2 wave of  comparable amplitude to the incident mode-1 wave is formed from a depression incident mode-1 wave; strong feedback enhances the polarity change process of the mode-1 wave. In (C) a concave mode-2 wave of large wave amplitude with wave fission is produced by an elevation incident mode-1 wave; strong feedback from the mode-2 wave suppresses the fission of the mode-1 wave. In all cases, the amplitudes of the generated mode-2 waves are proportional to the topographic slope.</p>