miR156-mediated changes in leaf composition lead to altered photosynthetic traits during vegetative phase change

SummaryPlant morphology and physiology change with growth and development. Some of these changes are due to change in plant size and some are the result of genetically programmed developmental transitions. In this study we investigate the role of the developmental transition, vegetative phase change (VPC), on morphological and photosynthetic changes.We used overexpression of miR156, the master regulator of VPC, to modulate the timing of VPC in Populus tremula x alba, Zea mays and Arabidopsis thaliana to determine its role in trait variation independent of changes in size and overall age.Here we find that juvenile and adult leaves in all three species photosynthesize at different rates and that these differences are due to phase-dependent changes in specific leaf area (SLA) and leaf N but not photosynthetic biochemistry. Further, we found juvenile leaves with high SLA were associated with better photosynthetic performance at low light levels.This study establishes a role for VPC in leaf composition and photosynthetic performance across diverse species and environments. Variation in leaf traits due to VPC are likely to provide distinct benefits under specific environments and, as a result, selection on the timing of this transition could be a mechanism for environmental adaptation.

Soil and foliar zinc biofortification of broccolini: effects on plant growth and mineral accumulation

Millions of people have Zn-deficient diets, so Zn-biofortified crops could prevent such deficiency. The aim of this study was to evaluate the use of agronomic Zn biofortification of broccolini – a new hybrid crop variety derived from a cross between kalian cabbage and broccoli. Plants were grown in pots using a Zn deficient soil. Four fertiliser treatments were tested: (1) control; (2) soil application of 5 mg ZnSO4•7H2O kg–1 soil; (3) foliar application at the early flowering stage of 0.5% (w/v) ZnSO4•7H2O; (4) combined soil and foliar treatments. Florets were harvested in four sequential harvests. There was a decrease in both growth and leaf composition of Zn, Ca, Fe and Mg. Soil Zn application increased floret production. There were increases in the Zn concentration stem+leaves and florets of 12- and 2.5-fold in foliar and soil+foliar treatments respectively. PA:Zn molar ratios decreased under both foliar and soil+foliar treatments. Boiling reduced Zn concentration by 40%, along with a decrease of other mineral nutrients. A soil+foliar treatment can increase both plant growth and Zn concentration in broccolini, and boiled 100 g portion of biofortified florets fertilised at rates in this study would deliver ~49 mg Zn, a 46% increase than in the non-biofortified broccolini.

Biofertilizers in horticultural crops

Bovine manure biofertilizers can exert physical, chemical, and biological effects on the soil and the phytoprotective, physiological, and productive actions in horticultural crops. The literature review was performed to compile information on the production, uses, and effects of bovine manure biofertilizers on soil properties and plants. Biofertilizers are produced by organic components, minerals, and inoculants. Among the organic sources, the bovine manure is the most used. The fermentation of the bovine manure in water constitutes the Vairo biofertilizer, the most employed in Brazilian horticulture. With the chemical enrichment of this preparation came the Supermagro, Agrobio, and Agrobom biofertilizers. In the soil, it can act in the improvement of soil fertility, physical properties, and in the diversity and abundance of the biota. Also, it can act in control of pests and diseases, leaf composition concerning macro and micronutrients, and in the production and post-harvest quality of horticultural crops. Therefore, this review describes the preparation, chemical composition, and utilization of bovine manure biofertilizers in both soil and plants, offering perspectives of research on the formulations, application, and effects of these inputs on horticultural species.

Sunspots are Correlated with Foliar pH in Grapevines

Foliar pH is a specific multifaceted parameter that is sensitive to a deficit in soil water and to temperature variations. It also represents a tool that can be used to rapidly phenotype the symbiosis induced in several crops by bio-fertilizers containing Arbuscular Mycorrhizal Fungi. Yearly decreases in foliar pH, which dropped from 3.73 in 2015 to 3.15 in 2017 and then stabilized at around 3.13, have been observed in an experimental vineyard near Torino (Italy) in six grapevine cultivars. In this paper, these curious, original results have been paired with the average sunspots of the 24th sun cycle, proximal to its endpoint. The paired values were highly correlated (r 0.95 P< 0.01), with close parabolic patterns. A lowering in foliar pH has been correlated with a modification of the leaf composition, as characterized by the higher hydration and reinforced wall. An increase in the circulating acidity of the plants has been hypothesized to interfere in a diminution in the general predisposition to block parasite attacks. From this perspective, the retrieval of several historic outbreaks and the long-term systematic monitoring of mud and Erwinia amylovora frequencies have suggested that the hypothesis that links the solar minima with dysfunctions of the plant-pest relationships cannot always be rejected. Cosmic influences pertaining to UV variations are poorly understood in plant physiopathology. Foliar pH appears to be a rapid and simple tool to unveil high-level mechanisms. It is this simple parameter that physiologists and geneticists, but also agronomists, are asked to consider.

FOLIAR COMPOSITION OF Eucalyptus urograndis IN TWO SITES FERTILIZED WITH N, P AND K IN SOUTHERN BRAZIL

The leaf composition has been used as a tool in establishing the nutritional diagnosis and evaluation of the effectiveness of the use of nutrients. This knowledge is still scarce in subtropical conditions of cultivation. In this sense, the study aims to evaluate the effect of N, P and K fertilization on leaf concentration of Eucalyptus urograndis. The experiments were installed in the Oxisols of the sandy-loam texture, in the counties of Jaguariaíva and Ventania, state of Paraná. In the N and P experiments, the following doses: 0; 30; 60; 120 and 240 kg ha-1 of N and P2O5; and 0; 35; 70; 140 and 280 kg ha-1 K2O, split in four applications: during planting and side dressing at 3, 9 and 12 months of age. The response was evaluated according to the nutritional status of the plants at 12, 24 and 36 months, by foliar concentration. It also evaluated levels of K deficiency plant through visual and chemical analysis. The application of N and P did not result in alteration in the leaf contents of the same in all evaluated periods and in the two regions, with a slight decrease in the concentrations with time. A different effect occurred with K at the Jaguariaíva site, which altered its foliar concentration at the 24th and 36th month, and increased foliar concentrations with time. These effects demonstrate that foliar diagnosis, through foliar concentrations, may present limitations of interpretation and should not be evaluated separately for fertilization recommendation.

Effects of water salinity and organomineral fertilization on leaf composition and production in Passiflora edulis

ABSTRACT An experiment was undertaken in Remígio County, Paraíba State, Brazil, from July 2013 to May 2014, in order to evaluate the effects of saline water irrigation, bovine biofertilizer, and potassium type on soil salinity, leaf macronutrient composition, and production of yellow passion fruit cv. BRS Gigante Amarelo. Treatments were distributed in randomized blocks, arranged in a 2 × 2 × 2 factorial design, with reference to electrical conductivity of the water (0.35 and 4.00 dS m-1), soil with and without bovine biofertilizer, and application of potassium chloride as a conventional treatment (KCl) and in an organic polymer-coated form, supplied monthly. Bovine biofertilizer was diluted in non-saline water (proportion, 50%) and applied via water at a volume of 6 L plant-1 one day before transplanting, and then every 90 days. The combination of saline water with bovine biofertilizer raised soil salinity to a similar proportion when comparing saline water and conventional potassium chloride with saline water and polymer-coated potassium chloride. The increase in water saline concentrations associated with both types of potassium chloride and with bovine biofertilizer elevated soil salinity from non-saline to saline. On starting to flower, plants of cv. BRS Gigante Amarelo were deficient in macronutrients other than nitrogen and potassium, but nonetheless produced fruits of an adequate mass for the consumer market.

Effects of nitrogen fertilization and nitrification inhibitor product on vegetative growth, production and oil quality in ‘Arbequina’ hedgerow and ‘Picual’ vase-trained orchards

Two experiments were carried out in olive orchards in the center of Spain over a three-year period. In this cold and dry area, growers traditionally apply large amounts of N with no experimental knowledge. An ‘Arbequina’ hedgerow and ‘Picual’ vase orchards were fertilized with two N-doses applied to the soil in spring with or without the nitrification inhibitor (DMPP). Vegetative growth, fruit and oil characteristics were evaluated. These variables were affected by the N-treatment during the 3rd year. The lowest N-application increased vegetative growth, while when N-leaf composition was higher than 2%, fruit dry weight, oil content and oil phenol content were reduced. ‘Picual’ did not respond to N-applications. The effect of DMPP on growth or production was not consistent and a lower phenolic content was obtained for ‘Arbequina’. Our results demonstrated that in this dry land, N-fertilization is not always necessary and oil quality can be negatively affected with high doses.