(1) Background: Evaluation of the quality and reliability of the frailty syndrome videos available on YouTube platform was the aim of this study. (2) Methods: The observational study included 75 videos retrieved by searching seven terms related to frailty syndrome on YouTube. The quality and reliability of the videos were measured using three different tools: quality criteria for consumer health information (DISCERN), the Global Quality Score (GQS), and the Journal of American Medical Association (JAMA). The video content was categorized according to the following characteristics: video provider, duration, view count, average daily views of the video, average daily views of a channel, channel subscribers, number of days since upload date, likes, dislikes, comments, the external webpages linked to the videos. (3) Results: The videos had a mean duration of 375 s and an average number of views of 1114. The quality of 17 videos assessed in the study was found to be high, 48—intermediate, and 10—low. The high-quality videos had the longest duration, the highest number of views, and points for the DISCERN score. The physician uploaders had the highest mean DISCERN and mean GQS scores, the highest number of views, and the longest duration but the hospital channels had the highest JAMA score. (4) Conclusions: YouTube can be a valuable source of medical information for patients and caregivers. The quality of videos mostly depends on the authorship and the source of video providers—physicians, academic, and health care-related organizations provide the best quality content based on professional medical knowledge.