Decolorization of Remazol Brilliant Violet 5R and Procion Red MX-5B by Trichoderma Species

Industrial wastewater including dye waste disposal, has been released in a massive amount and is difficult to degrade, especially synthetic dyes. In this study, 10 different types of fungi were isolated from a decayed wood in UTM forest and were labelled as S1-S10. Two dyes were chosen for this study, which were Procion Red MX-5B (PRMX5B) and Remazol Brilliant Violet 5R (RBV5R). These fungi were screened for their ability to decolor both dyes and further tested for their ability to decolor the dyes in liquid medium under several parameters; carbon and nitrogen sources, initial pH value, temperature, and agitation. S1 decolorized PRMX5B efficiently with the addition of glucose (45%), ammonium nitrate (61%), pH 3 (69%), temperature 37°C (49%), and agitation 100 rpm (69%), whereas S2 decolorized efficiently with the addition of glucose (60%), ammonium nitrate (49%), pH 3 (70%), temperature 37°C (46%), and agitation 100 rpm (74%). S1 demonstrated efficient decolorization of RBV5R with the addition of glucose (80%), ammonium nitrate (62%), pH 3, temperature 37°C (75%), and agitation 100 rpm (90%), whereas S2 demonstrated efficient decolorization with the addition of glucose (52%), ammonium nitrate (67%), pH 3, temperature 37°C (75%), and agitation 100 rpm (71%).The percentage of decolorization of dyes was measured by using a UV-Vis spectrophotometer. These fungi were then identified using the 18sr RNA method. Based on macroscopic and microscopic characteristics and a polygenetic tree, fungi S1 belong to Trichoderma koningiopsis and fungi S2 belong to Trichoderma atroviride.

Adsorption of Remazol Brilliant Violet 5R (RBV-5R) and Remazol Brilliant Blue R (RBBR) from Aqueous Solution by Using Agriculture Waste

The ability of agricultural waste materials to remove synthetic dyes such as Remazol Brilliant Violet 5R (RBV-5R) and Remazol Brilliant Blue R (RBBR) from aqueous solutions was investigated. Dyes are a major source of water contamination that not only cause significant damage to water bodies but also have a negative effect on human health due to their high toxicity and carcinogenic nature. Agricultural wastes are renewable adsorbents because they are readily available and inexpensive, and they can also be used instead of conventional activated carbon. As a result, the removal of RBV-5R and RBBR from dye solutions by adsorption onto treated adsorbent was investigated in this review. The two best adsorbents out of ten were selected via a screening process with RBBR as the test dye. The key adsorbents in this analysis were coconut shells and mango seeds, which had the highest removal rate as compared to others. The experiment was continued with the chosen adsorbent to see how different initial dye concentrations, adsorbent dosage, contact time, pH, and particle size affected dye adsorption. The results show that different parameters have different effects on the removal rate and adsorption potential of the adsorbent. The adsorption of dye from aqueous solution onto adsorbent was investigated using Fourier transform infrared spectroscopy (FTIR) to investigate the functional groups of the adsorbent before and after the adsorption operation, and it was discovered that the functional group affected the effectiveness or removal rate as well as the adsorption capability of adsorbents. According to the findings, 5 gram mango seeds can extract 85.54 percent of RBV-5R with adsorption power of 1.26 mg/g. For 21 hours, coconut shells removed 74.39 percent of RBBR with an adsorption capacity of 8.01 mg/g. The findings indicated that these agricultural wastes could be useful as an alternative adsorbent for removing dye from aqueous solutions.

Biodecolorization of Azo Dye Mixture (Remazol Brilliant Violet 5r and Reactive Red 120) by Indigenous Bacterial Consortium Obtained From Dye Contaminated Soil

Discharge of the untreated wastewater containing dyestuff into the surrounding aquatic environment is of significant environmental concern. These dying effluents not only change the color of water bodies but also has many unfavorable conditions and release toxic by-products, which are mutagenic, carcinogenic, and hazardous to different life forms. The present study investigated the biodegradation and removal of dye mixture (Remazol Brilliant violet 5R and Reactive Red 120) using a new bacterial consortium isolated from dye contaminated soil. Among the total 15 isolates screened, the two most efficient bacterial species (SS07 and SS09) were selected and identified as Enterobacter cloacae (MT573884) and Achromobacter pulmonis (MT573885) through biochemical assays and 16S rRNA gene sequencing. The removal efficiency of dye mixture by Enterobacter cloacae and Achromobacter pulmonis at an initial concentration of 100 mg L− 1 was 82.78 and 84.96%, discretely. The bacterial consortium was developed using selected isolates, and the optimum conditions for the removal of dyes were investigated by studying the effects of pH, temperature, carbon and nitrogen sources, dye concentration, and inoculum size. The maximum decolorization efficiency was achieved at pH, 7; temperature, 37°C; dye concentration, 100 ppm; and initial inoculum concentration, 0.5 ml, respectively. Mannitol and Ammonium sulfate was identified as the most suitable carbon and nitrogen sources for better bacterial growth and decolorization. The maximum removal efficiency of 91.3% achieved at the optimal conditions after 72 h of incubation. Decolorization of azo dyestuff by the developed microbial consortia conforms to the zero-order reaction kinetics model. Consortia of Enterobacter cloacae and Achromobacter pulmonis was established as an effective decolorizer for the Remazol Brilliant violet 5R and Reactive Red 120 dye mixture with > 90% color removal.

Functionalized Stink Bean Pod (Parkia speciosa) Powder for Adsorption of Reactive Dye

The potential of agricultural waste materials for removing synthetic dye, Remazol Brilliant Blue R (RBBR) and Remazol Brilliant Violet 5R (RBV5) from aqueous solution were investigated. Water pollution is one of the major environmental issues; dyes have contributed enormous damage to water bodies and greatly impact human health due to high toxicity and carcinogenic by nature. Agricultural wastes are sustainable adsorbents and generally low cost, which has the potential to replace traditional activated carbon. Therefore, this study investigated the removal of RBBR and RBV5 dye from the aqueous solution by adding adsorbent through adsorption. Stink bean pod (Parkia speciosa) was selected as the main adsorbent of this study. The experiment was carried out to study the effect of different initial dye concentration, adsorbent dosage, contact time, and pH level of dye solution. The results have shown that different parameters can affect the removal rate and adsorption capacity of adsorbents in different ways. The adsorption of dye from aqueous solution onto adsorbent was assessed using Fourier Transform Infrared Spectroscopy (FTIR) to determine the functional groups of adsorbent before and after the adsorption process. The FTIR results show that the functional group would affect the removal rate and adsorption capacity of adsorbents. The present study indicates that 83.35% of RBBR with the adsorption capacity of 1.23 mg/g was removed by a 3 g stink bean pod. In comparison, 80.07% of RBV5 with the adsorption capacity of 1.22 mg/g was removed by a 3 g stink bean pod. Design-Expert software was used to generate the equation, which represents the behavior for the adsorption of RBBR and RBV5 dye. The results proved that stink bean pod was promising material as an alternative adsorbent for the removal of dye from aqueous solution.