The effect of tapioca flour and milk powder on the quality of analog mushroom processed from surimi

Surimi is a myofibril protein concentrate that can be processed into various innovative analog products. Innovation surimi research into analog mushroom products was formulated with tapioca flour (12% & 15% ) and milk powder (2.5% & 5%) has been done. The surimi that was formulated using both ingredients and other material was molded and steamed to become a surimi jelly in mushroom shape. Observations of analog mushroom were made on proximate analysis, pH, texture profile, colour, and sensory evaluation. The results showed that the more tapioca concentrations added, will affect of the decrease of ash and protein content, texture profile value (springiness, cohesiveness), and sensory value (appearance, texture and taste), but increase of hardness, brightness (L* value) and yellowness (+b*) of analog mushrooms. While the addition of concentration milk powder has an effect increasing of fat content, brightness (L* value) and texture profile value (gumminess & chewingness). Mushroom analogs treated with 12% tapioca flour and 5% milk powder produce analog mushroom that have physical (lightness, L* value), texture profile value (hardness, springeness, cohesiveness, gummines and chewingness) and sensory properties (texture and taste) better compared to other treatments

Effect of conjugated protein composed of porcine myofibril and Protaetia brevitarsis protein on protein functionality

The replacement of conventional protein supply has been studied in the context of continuous population growth and the consequent environmental problems. Among protein substitutes, edible insects have gained relevance due to their high breeding efficiency. This study was conducted to estimate the effect of the partial replacement of porcine myofibril protein (pork protein; PP) with protein extracted from Protaetia brevitarsis (insect protein; IP) in protein-containing solutions and gels. The protein concentration was regulated at 30 and 1 mg/ml for gels and solutions, respectively, and the replacement percentages were increased by 20%. Gels containing IP showed a lower viscosity and thermal stability; the texture and surface of the gels were negatively impacted as the replacement percentage increased. Therefore, heating is not an appropriate method to induce the gelation of IP containing solutions. However, the replacement of PP with IP increased the foam capacity and emulsion capacity in solutions. In conclusion, we show that the partial replacement of PP with IP positively impacts on protein solutions but impairs their gelation.

Properties of heat induced blend gel of Peruvian squid (Dosidicus gigas) myofibril protein fortified with millet (Panicum miliaceum L.) flour

Weak gelation ability and simple nutrient composition of squid myofibril protein limits its further development in surimi production. Millet flour is rich in nutrients and has a good viscoelasticity after heating. Physicochemical properties of Peruvian squid myofibril protein based blend gel fortified with millet four were studied. Rheological test results showed that all blend gels exhibit solid-like characters. With the addition of millet flour, the composite modulus of all the samples showed a trend of decreasing first and then increasing. The minimum value appeared around 48oC. Moreover, it was found that gel strength, water holding capacity, hardness, gumminess and chewiness showed a trend of increasing at first and then decreasing. The blend gel with 4% millet flour addition exhibit good performance in those testing. The subsequent analysis of water distribution further explained and proved the mechanism. In summary, the blend gel of squid myofibril protein fortified with 4% of millet flour addition was considered the most suitable amount for enhancing the quality of blend gel.

NILAI ORGANOLEPTIK BAKSO IKAN LAYANG (Decapterus russelli), IKAN KUNIRAN (Upeneus moluccensis) DAN IKAN NILA (Oreochromis niloticus)

Bakso ikan merupakan produk berbahan dasar dari surimi yang terbuat dari lumatan daging ikan yang telah mengalami proses penghilangan tulang, dan sebagian komponen larut air dan lemak melalui pencucian dengan air, sehingga disebut sebagai konsentrat basah protein myofibril dari daging ikan layang, kuniran, dan nila. Tujuan penelitian adalah untuk mengetahui mutu bakso dari berbagai jenis daging ikan yang digunakan. Metode yang digunakan dalam penelitian ini adalah eksperimental. Hasil uji ogranoleptik menunjukkan bakso dengan kategori-kategori berikut, kenampakan terbaik pada ikan nila (8,2), bau terbaik dari ikan kuniran (7,8), rasa terbaik pada ikan layang (8,8) dan tekstur terbaik pada ikan layang (8,8). Kesimpulan dari ketiga jenis bahan baku yang digunakan ikan kuniran merupakan bahan baku terbaik dalam pembuatan bakso. Fish ball is a surimi-based product made from ground fish meat that has undergone a bone removal process as well as elimination of water and fat soluble components through washing with water. Hence, it is called wet concentrated myofibril protein mainly derived from mackerel scad, goldband goatfish, and tilapia meat. The objective of this research was to determine the quality of fish balls from various types of fish (mackerel scad, mac). Organoleptic tests showed that the best appearance, smell, taste and texture categories were found in tilapia (8.2), goatfish (7.8), mackerel scad (8.8), and mackerel scad (8.8) respectively. As a conclusion, of the three raw materials used in this research, goldband goatfish proved to be the best source of raw material for fish balls.

Early changes of energy metabolism, isoformic content and level of titin phosphorylation at diastolic dysfunction

Relevance. Diastolic dysfunction occurring at hypertension, obesity, diabetes, or treatment with doxorubicin tends to prevail in all patterns of chronic heart failure. Lack of effective therapy forces to look more into the metabolic processes in cardiomyocytes.Objective. Assess energy metabolism in cardiomyocytes and changes in titin, a giant myofibril protein that responsible for their elasticity.Material and Methods. The study model was cardiomyopathy occurring after the 4-week administration of doxorubicin (2 mg/kg weekly). Diastolic dysfunction was identified by echocardiography and catheterization with the simultaneous measurement of pressure and volume of the left ventricle (LV).Results. The levels of adenine nucleotides and phosphocreatine in the heart of animals treated with doxorubicin differed little from the normal values, but lactate levels were increased manifold. A 50% increase in the level of titin phosphorylation was detected, which correlated (r = 0,94) with a nearly twofold increase in the share of a more elastic N2BA-isoform of this protein.Conclusion. This form of diastolic dysfunction involves the activation of anaerobic metabolism and increased stretching of myofibrils facilitating LV filling.