scholarly journals Raman spectroscopy enables phenotyping and assessment of nutrition values of plants: a review

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
William Z. Payne ◽  
Dmitry Kurouski
Keyword(s):  
Raman Spectroscopy ◽  
Abiotic Stresses ◽  
Molecular Techniques ◽  
Plant Diseases ◽  
Label Free ◽  
Breeding Programs ◽  
Non Invasive ◽  
Nutrition Value ◽  
Non Destructive ◽  
Selection Of

AbstractOur civilization has to enhance food production to feed world’s expected population of 9.7 billion by 2050. These food demands can be met by implementation of innovative technologies in agriculture. This transformative agricultural concept, also known as digital farming, aims to maximize the crop yield without an increase in the field footprint while simultaneously minimizing environmental impact of farming. There is a growing body of evidence that Raman spectroscopy, a non-invasive, non-destructive, and laser-based analytical approach, can be used to: (i) detect plant diseases, (ii) abiotic stresses, and (iii) enable label-free phenotyping and digital selection of plants in breeding programs. In this review, we critically discuss the most recent reports on the use of Raman spectroscopy for confirmatory identification of plant species and their varieties, as well as Raman-based analysis of the nutrition value of seeds. We show that high selectivity and specificity of Raman makes this technique ideal for optical surveillance of fields, which can be used to improve agriculture around the world. We also discuss potential advances in synergetic use of RS and already established imaging and molecular techniques. This combinatorial approach can be used to reduce associated time and cost, as well as enhance the accuracy of diagnostics of biotic and abiotic stresses.

scholarly journals Non-Invasive Identification of Nutrient Components in Grain

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3124
Author(s):  
Charles Farber ◽  
A. S. M. Faridul Islam ◽  
Endang M. Septiningsih ◽  
Michael J. Thomson ◽  
Dmitry Kurouski
Keyword(s):  
Rice Variety ◽  
Nutrient Content ◽  
Plant Diseases ◽  
Variety Identification ◽  
Successful Implementation ◽  
Label Free ◽  
Accurate Identification ◽  
Analytical Technique ◽  
Non Invasive ◽  
Digital Farming

Digital farming is a modern agricultural concept that aims to maximize the crop yield while simultaneously minimizing the environmental impact of farming. Successful implementation of digital farming requires development of sensors to detect and identify diseases and abiotic stresses in plants, as well as to probe the nutrient content of seeds and identify plant varieties. Experimental evidence of the suitability of Raman spectroscopy (RS) for confirmatory diagnostics of plant diseases was previously provided by our team and other research groups. In this study, we investigate the potential use of RS as a label-free, non-invasive and non-destructive analytical technique for the fast and accurate identification of nutrient components in the grains from 15 different rice genotypes. We demonstrate that spectroscopic analysis of intact rice seeds provides the accurate rice variety identification in ~86% of samples. These results suggest that RS can be used for fully automated, fast and accurate identification of seeds nutrient components.

scholarly journals Nanoscale mapping of newly-synthesised phospholipid molecules in a biological cell using tip-enhanced Raman spectroscopy

2017 ◽  
Vol 53 (16) ◽  
pp. 2451-2454 ◽  
Author(s):  
Naresh Kumar ◽  
Marek M. Drozdz ◽  
Haibo Jiang ◽  
Daniela M. Santos ◽  
David J. Vaux
Keyword(s):  
Raman Spectroscopy ◽  
Biological Cell ◽  
Label Free ◽  
Tip Enhanced Raman Spectroscopy ◽  
First Time ◽  
Non Destructive

Non-destructive & label-free nanoscale mapping of newly-synthesised phospholipid molecules inside a biological cell is demonstrated using tip-enhanced Raman spectroscopy for the first time.

Alteration Features of Stones Applied in Underground Metro Stations

2010 ◽  
Vol 636-637 ◽  
pp. 1292-1299 ◽  
Author(s):  
Carlos Alves ◽  
Carlos Figueiredo ◽  
Paula Figueiredo ◽  
António Maurício ◽  
Luís Aires-Barros
Keyword(s):  
Field Study ◽  
Visual Inspection ◽  
Rock Type ◽  
Surface Irregularity ◽  
Non Invasive ◽  
Stone Materials ◽  
Complete Study ◽  
Stone Characteristics ◽  
Non Destructive ◽  
Selection Of

Several types of stones (igneous, sedimentary and metamorphic) are found applied in pavements, stairs and walls of the underground stations of the Lisbon Metropolitan System, Portugal. A field study based solely on non-destructive and non invasive visual inspection of these stations is presented, aiming to identify different transformations of applied stones and discuss its relation with the stone characteristics. This field study has pointed to relevant issues regarding durability of stone materials namely when used in pavements and stairs. The most striking aspect is the frequently marked variable behaviour of stones from a given rock type under the same use conditions, with heterogeneities and discontinuities that seem to cause irregularity from the early stages and further increase with time, having more accentuated consequences (in terms of intensity of surface irregularity) in some types of limestones. The observations collected could be relevant for the discussion of characteristics relevant for the quality control of these materials (namely at the quarry level), including the selection of stones for future substitutions. There is, therefore, the need for a more complete study comprising detailed petrographic studies and laboratory tests both from the applied stones and from the supplying quarries.

Beyond Death: Forensic Investigations of pre-Columbian Mummies from the Tarapacá Valley, Chile, Using Variable Pressure SEM and Raman Spectroscopy

2007 ◽  
Vol 15 (6) ◽  
pp. 6-11 ◽  
Author(s):  
S.V. Prikhodko ◽  
C. Fischer ◽  
R. Boytner ◽  
M. C. Lozada ◽  
M. Uribe ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Chemical Characterization ◽  
Quantitative Information ◽  
Mortuary Practices ◽  
Variable Pressure ◽  
Scientific Investigations ◽  
Non Invasive ◽  
Archaeological Materials ◽  
Non Destructive

Variable pressure scanning electron microscopy (VPSEM) coupled with other non-destructive analytical methods, such as energy dispersive (EDS) and Raman spectroscopy (RS) offers new capabilities for non-invasive imaging and chemical characterization of archaeological materials. This article underlines the application of VPSEM-EDS-RS on bioarchaeological specimens of pre-Columbian mummies from the Tarapacá Valley in northern Chile. The aim of the scientific investigations is to identify nonanatomical features and to provide qualitative and quantitative information at molecular levels, complementing the morphological record from studies in physical anthropology, in an effort to understand mortuary practices in the Tarapacá Valley and the effects of the burial environment in the preservation of mummified human remains.

Exploring the structure and formation mechanism of amyloid fibrils by Raman spectroscopy: a review

The Analyst ◽  
2015 ◽  
Vol 140 (15) ◽  
pp. 4967-4980 ◽  
Author(s):  
Dmitry Kurouski ◽  
Richard P. Van Duyne ◽  
Igor K. Lednev
Keyword(s):  
Raman Spectroscopy ◽  
Formation Mechanism ◽  
Structural Characterization ◽  
Amyloid Fibrils ◽  
Label Free ◽  
Amyloidogenic Proteins ◽  
Non Destructive

Applications of Raman spectroscopy, a label-free non-destructive technique, for the structural characterization of amyloidogenic proteins, prefibrilar oligomers, and mature fibrils.

scholarly journals Espectroscopia Raman e a Ciência do Patrimônio: aspectos gerais e panorama atual na América do Sul

2018 ◽  
Vol 15 (30) ◽  
pp. 344
Author(s):  
Dalva Lucia Araujo Faria
Keyword(s):  
Raman Spectroscopy ◽  
Cultural Heritage ◽  
South American ◽  
Research Groups ◽  
Chemical Methods ◽  
Non Invasive ◽  
Inelastic Light Scattering ◽  
Physico Chemical ◽  
American Research ◽  
Non Destructive

As últimas décadas tem presenciado um crescente aumento no uso de métodos físico-químicos de análise de bens culturais. Algumas dessas técnicas tem se mostrado particularmente úteis pelo caráter não invasivo e não destrutivo que apresentam e, dentre elas, a espectroscopia Raman apresenta posição de destaque. Neste artigo, essas características da técnica são detalhadas, bem como são apresentados os fundamentos teóricos do espalhamento inelástico de luz e discutidos alguns aspectos práticos de sua utilização. Finalmente, é apresentado um levantamento detalhado dos grupos de pesquisa da América do Sul, voltados ao estudo de bens culturais, que tem se utilizado dessa ferramenta analítica e em qual tipo de questionamentos vem sendo aplicada. Abstract: The last decades have witnessed an ever growing increase in the usage of physico-chemical methods to investigate cultural heritage problems. Some of these techniques are proving to be particularly useful in such a context, due to their non-invasive and non-destructive nature; among them, Raman spectroscopy occupies a prominent position. In this paper, such characteristics of the technique are detailed and the physical basis of inelastic light scattering are presented, together with some practical aspects of its applications. Finally, it is here offered a detailed survey in the literature on the South American research groups devoted to the investigation of cultural heritage issues who are using Raman spectroscopy, and the type of interrogation carried on. 

scholarly journals High-Throughput Phenotyping Approach for Screening Major Carotenoids of Tomato by Handheld Raman Spectroscopy Using Chemometric Methods

Sensors ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 3723 ◽  
Author(s):  
Hacer Akpolat ◽  
Mark Barineau ◽  
Keith A. Jackson ◽  
Mehmet Z. Akpolat ◽  
David M. Francis ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Performance ◽  
Prediction Models ◽  
Least Squares Regression ◽  
Family Farms ◽  
Non Invasive ◽  
Uv Visible ◽  
The Ohio State University ◽  
Non Destructive ◽  
Β Carotene

Our objective was to develop a rapid technique for the non-invasive profiling and quantification of major tomato carotenoids using handheld Raman spectroscopy combined with pattern recognition techniques. A total of 106 samples with varying carotenoid profiles were provided by the Ohio State University Tomato Breeding and Genetics program and Lipman Family Farms (Naples, FL, USA). Non-destructive measurement from the surface of tomatoes was performed by a handheld Raman spectrometer equipped with a 1064 nm excitation laser, and data analysis was performed using soft independent modelling of class analogy (SIMCA)), artificial neural network (ANN), and partial least squares regression (PLSR) for classification and quantification purposes. High-performance liquid chromatography (HPLC) and UV/visible spectrophotometry were used for profiling and quantification of major carotenoids. Seven groups were identified based on their carotenoid profile, and supervised classification by SIMCA and ANN clustered samples with 93% and 100% accuracy based on a validation test data, respectively. All-trans-lycopene and β-carotene levels were measured with a UV-visible spectrophotometer, and prediction models were developed using PLSR and ANN. Regression models developed with Raman spectra provided excellent prediction performance by ANN (rpre = 0.9, SEP = 1.1 mg/100 g) and PLSR (rpre = 0.87, SEP = 2.4 mg/100 g) for non-invasive determination of all-trans-lycopene in fruits. Although the number of samples were limited for β-carotene quantification, PLSR modeling showed promising results (rcv = 0.99, SECV = 0.28 mg/100 g). Non-destructive evaluation of tomato carotenoids can be useful for tomato breeders as a simple and rapid tool for developing new varieties with novel profiles and for separating orange varieties with distinct carotenoids (high in β-carotene and high in cis-lycopene).

scholarly journals Label-free Raman monitoring of extracellular matrix formation in three-dimensional polymeric scaffolds

2013 ◽  
Vol 10 (86) ◽  
pp. 20130464 ◽  
Author(s):  
Aliz Kunstar ◽  
Anne M. Leferink ◽  
Paul I. Okagbare ◽  
Michael D. Morris ◽  
Blake J. Roessler ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Raman Spectroscopy ◽  
Three Dimensional ◽  
Raman Microspectroscopy ◽  
Collagen Content ◽  
Cartilage Tissue ◽  
Fibre Optic ◽  
Label Free ◽  
Tissue Quality ◽  
Non Invasive

Monitoring extracellular matrix (ECM) components is one of the key methods used to determine tissue quality in three-dimensional scaffolds for regenerative medicine and clinical purposes. Raman spectroscopy can be used for non-invasive sensing of cellular and ECM biochemistry. We have investigated the use of conventional (confocal and semiconfocal) Raman microspectroscopy and fibre-optic Raman spectroscopy for in vitro monitoring of ECM formation in three-dimensional poly(ethylene oxide terephthalate)–poly(butylene terephthalate) (PEOT/PBT) scaffolds. Chondrocyte-seeded PEOT/PBT scaffolds were analysed for ECM formation by Raman microspectroscopy, biochemical analysis, histology and scanning electron microscopy. ECM deposition in these scaffolds was successfully detected by biochemical and histological analysis and by label-free non-destructive Raman microspectroscopy. In the spectra collected by the conventional Raman set-ups, the Raman bands at 937 and at 1062 cm −1 which, respectively, correspond to collagen and sulfated glycosaminoglycans could be used as Raman markers for ECM formation in scaffolds. Collagen synthesis was found to be different in single chondrocyte-seeded scaffolds when compared with microaggregate-seeded samples. Normalized band-area ratios for collagen content of single cell-seeded samples gradually decreased during a 21-day culture period, whereas collagen content of the microaggregate-seeded samples significantly increased during this period. Moreover, a fibre-optic Raman set-up allowed for the collection of Raman spectra from multiple pores inside scaffolds in parallel. These fibre-optic measurements could give a representative average of the ECM Raman signal present in tissue-engineered constructs. Results in this study provide proof-of-principle that Raman microspectroscopy is a promising non-invasive tool to monitor ECM production and remodelling in three-dimensional porous cartilage tissue-engineered constructs.

Archaeological investigations (archaeometry)

2018 ◽  
Vol 3 (9) ◽  
Author(s):  
Anastasia Rousaki ◽  
Luc Moens ◽  
Peter Vandenabeele
Keyword(s):  
Raman Spectroscopy ◽  
Organic Materials ◽  
Research Area ◽  
Fundamental Interest ◽  
Non Invasive ◽  
Wide Range ◽  
Research Questions ◽  
Non Destructive ◽  
Speed Of Analysis ◽  
Straightforward Interpretation

Abstract Archaeometry is the research area on the edge between humanities and natural sciences: it uses and optimises methods from chemistry, spectroscopy, physics, biology, etc. to help answering research questions from humanities. In general, these objects are investigated for several reasons. Besides the fundamental interest to know about the materials that were used in the past, the study of artefacts can support their preservation, either by helping to select optimal storage or display conditions, either by investigating decay pathways and suggesting solutions. Other reasons for art analysis include provenance studies, dating the artefact or identifying forgeries. Since several years, Raman spectroscopy is increasingly applied for the investigation of objects of art or archaeology. The technique is well-appreciated for the limited (or even absent) sample preparation, the relative straightforward interpretation of the spectra (by fingerprinting - comparing them against a database of reference pigments) and its speed of analysis. Moreover, the small spectral footprint – allowing to record a molecular spectrum of particles down to 1 µm, the typical size of pigment grains – is certainly a positive property of the technique. Raman spectroscopy can be considered as rather versatile, as inorganic as well as organic materials can be studied, and as the technique can gather information on crystalline as well as on non-crystalline phases. As a consequence, Raman spectroscopy can be used to study antique objects and twentieth-century synthetic (organic) materials – illustrating the wide range of applications. Finally, the technique is as non-destructive, provided the laser power is kept sufficiently low not to damage the artwork. In literature, the terms “non-invasive” and “non-destructive” are used, where the first term means that no sampling is involved, and the latter term indicates that no sample is taken or that during analysis the sample is not consumed (destroyed) and remains available for further analysis.

scholarly journals Raman Spectroscopy Enables Non-Invasive Identification of Mycotoxins p. Fusarium of Winter Wheat Seeds

Photonics ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 587
Author(s):  
Maksim N. Moskovskiy ◽  
Aleksey V. Sibirev ◽  
Anatoly A. Gulyaev ◽  
Stanislav A. Gerasimenko ◽  
Sergey I. Borzenko ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Winter Wheat ◽  
Accurate Identification ◽  
Non Invasive ◽  
Visual Identification ◽  
Seed Analysis ◽  
Spectroscopic Signatures ◽  
Non Destructive ◽  
Wheat Seeds

Identification of specific mycotoxins p. Fusarium contained in infected winter wheat seeds can be achieved by visually recognizing their distinctive phenotypic species. The visual identification (ID) of species is subjective and usually requires significant taxonomic knowledge. Methods for the determination of various types of mycotoxins of the p. Fusarium are laborious and require the use of chemical invasive research methods. In this research, we investigate the possibility of using Raman spectroscopy (RS) as a tag-free, non-invasive and non-destructive analytical method for the rapid and accurate identification of p. Fusarium. Varieties of the r. Fusarium can produce mycotoxins that directly affect the DNA, RNA and chemical structure of infected seeds. Analysis of spectra by RS methods and chemometric analysis allows the identification of healthy, infected and contaminated seeds of winter wheat with varieties of mycotoxins p. Fusarium. Raman seed analysis provides accurate identification of p. Fusarium in 96% of samples. In addition, we present data on the identification of carbohydrates, proteins, fiber and other nutrients contaminated with p. Fusarium seeds obtained using spectroscopic signatures. These results demonstrate that RS enables rapid, accurate and non-invasive screening of seed phytosanitary status.

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